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Extended BGK Boltzmann for Dense Gases

Communications in Computational Physics 2013年第3期13卷 629-648页

作者： Saikishan Suryanarayanan shiwani singh Santosh Ansumali Engineering Mechanics Unit Jawaharlal Nehru Centre for Advanced Scientific ResearchJakkurBangalore 560064India

An alternate BGK type formulation of the Enskog equation has been recently proposed[1].It was shown that the new model has a valid H-theorem and correct thermal *** propose Lattice Boltzmann(LB)formulation of this new EnskogBGK *** molecular nature of the model is verified in case of shear flow by comparing the predicted normal stress behavior by the current model with the prediction of molecular dynamics *** extend the model for multiphase flow by incorporating attractive part as Vlasov type *** validate multiphase formulation,the results of 3D simulations of a condensing bubble in a periodic box are presented.

关键词： Lattice Boltzmann method multiphase flow Enskog equation

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Energy Conserving Lattice Boltzmann Models for Incompressible Flow Simulations

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Communications in Computational Physics 2013年第3期13卷 603-613页

作者： shiwani singh Siddharth Krithivasan Iliya V.Karlin Sauro Succi Santosh Ansumali Engineering Mechanics Unit Jawaharlal Nehru Centre for Advanced Scientific ResearchJakkurBangalore 560064India Aerothermochemistry and Combustion Systems Lab ETH Zurich8092 ZurichSwitzerland Energy Technology Research Group School of Engineering SciencesUniversity of SouthamptonSouthamptonSO171BJUK Istituto Applicazioni Calcolo“Mauro Picone” C.N.R.Via dei Taurini1900185RomeItaly

In this paper,we highlight the benefits resulting from imposing energyconserving equilibria in entropic lattice Boltzmann models for isothermal *** advantages are documented through a series of numerical simulations,s... 详细信息

关键词： Lattice Boltzmann method

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Expression of TTV-ORF2 Protein for Detection of Anti-TTV IgG Antibodies in Human Sera

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Advances in Infectious Diseases 2013年第3期3卷 223-229页

作者： shiwani singh Akanksha singh Dhananjay singh Mankotia Kalpana Luthra Mohammad Irshad Clinical Biochemistry Division Department of Laboratory Medicine All India Institute of Medical Sciences New Delhi India

The present study describes the cloning and expression of ORF-2 region of TTV genome and the use of expressed peptide in developing immunoassay for detection of anti-TTV antibodies in serum. Presence of TTV-DNA in serum was detected by PCR amplifying N-22 region of ORF-1 of TTV genome. This was followed by genotyping of TTV by RFLP using N-22 amplicon. Using genotype-1 positive serum as the source of TTV, the ORF-2 region was amplified by PCR and subsequently cloned and expressed in pET-19b vector. The expressed protein, identified as 20 kDa protein on SDS-PAGE gel, was purified by affinity chromatography and then used as antigen to develop western blot assay for detection of anti-TTV antibodies in serum. Analysis of sera for anti-TTV antibodies and their comparison with presence of TTV-DNA, produced encouraging results. There was a good relation between presence of anti-TTV and TTV-DNA in these sera samples. Anti-TTV antibodies could be detected in all TTV-DNA positive sera irrespective of the presence of TTV-genotype. This investigation demonstrates that ORF-2 peptide may be used in developing immunoassay for identification of TTV infection.

关键词： TTV ORF-2 Anti TTV-IgG Expression N-22

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Torque teno virus:Its prevalence and isotypes in North India

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World Journal of Gastroenterology 2008年第39期14卷 6044-6051页

作者： Mohammad Irshad shiwani singh Khushboo Irshad Sanjay Kumar Agarwal Yogendra Kumar Joshi Clinical Biochemistry Division Department of Laboratory MedicineAll India Institute of Medical SciencesNew Delhi-110029India Department of Biochemistry All India Institute of Medical SciencesNew Delhi-110029India Department of Nephrology All India Institute of Medical SciencesNew Delhi-110029India Department of Gastroenterology and Human Nutrition All India Institute of Medical SciencesNew Delhi-110029India

AIM: To investigate the prevalence and genotype dis-tribution of Torque teno virus (TTV) in patients with different liver diseases and chronic renal failure treated at a referral hospital in North India. METHODS: Whereas prevalence of TTV was based on amplification of conserved region of ORF2 of TTV genome,the genotyping of TTV was carried out using restriction fragment length polymorphism (RFLP) procedure on the N22 region of ORF1. RESULTS: TTV-DNA was detected in 137 of 513 (26.7%) patients with liver diseases and 38 of 65 (58.5%) patients with chronic renal failure. TTV was also detected in 27% of healthy controls. The sequence analysis of the PCR product from 10 randomly selected cases failed to show a significant sequence divergence when compared with that of the TRM1 isolate of TTV genotype 1. The results of genotyping in 55 randomly selected patients showed the presence of genotype 1 (G1) in 53 (96.4%) and genotype 2 (G2) in 2 cases (3.6%),respectively. Other genotypes were not identified in this patient subgroup,suggesting that G1 is predominant in this area. The results of genotyping by RFLP were also supported by phylogenetic tree analysis,where G1 was found to be the major genotype. CONCLUSION: These results indicate that TTV is moderately present in Indian patients,with G1 to be the major genotype in North India. The pathogenicity and etiological role of TTV in different diseases is still a question mark and warrant further studies.

关键词：病毒感染基因型多态性印度

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