检索结果-南通市图书馆

维普期刊数据库

A core-shell structured COYID-19 mRNA vaccine with favorable biodistribution pattern and promising immunity

在线全文

学校读者我要写书评

暂无评论

Signal Transduction and Targeted Therapy 2021年第6期6卷 1920-1929页

作者： Ren Yang Yao Deng Baoying Huang Lei Huang Ang lin Yuhua li Wenling Wang Jingjing liu Shuaiyao Lu Zhenzhen Zhan Yufei Wang Ruhan A Wen Wang Peihua Niu li Zhao shiqiang li Xiaopin Ma Luyao Zhang Yujian Zhang Weiguo Yao Xingjie liang Jincun Zhao Zhongmin liu Xiaozhong Peng Hangwen li Wenjie Tan NHC Key Laboratory of Biosafety National Institute for Viral Disease Control and PreventionChinese Center for Disease Control and PreventionBeijingChina Stemirna Therapeutics ShanghaiChina National Institute for Food and Drug Control BeijingChina National Kunming High-level Primate Research Center Institute of Medical BiologyChinese Academy of Medical Sciences and Peking Union Medical CollegeYunnanChina Shanghai East Hospital Tongji UniversityShanghaiChina CAS Center for Excellence in Nanoscience CAS Key Laboratory for Biomedical Effects of Nanomaterials and NanosafetyChinese Academy of Sciences and National Center for Nanoscience and Technology of ChinaBeijingChina University of Chinese Academy of Sciences BeijingChina State Key Laboratory of Respiratory Disease National Clinical Research Center for Respiratory DiseaseGuangzhou Institute of Respiratory HealthThe First Affiliated Hospital of Guangzhou Medical UniversityGuangzhouGuangdongChina Center for Biosafety Mega-Science Chinese Academy of SciencesWuhanHubeiChina

Although inoculation of COVID-19 vaccines has rolled out globally,there is still a critical need for safe and effective vaccines to ensure fair and equitable supply for all countries.Here,we report on the development of a highly efficacious mRNA vaccine,SW0123 that is composed of sequence-modified mRNA encoding the full-length SARS-CoV-2 Spike protein packaged in core-shell structured lipopolyplex(LPP)nanoparticles.SWOT 23 is easy to produce using a large-scale microfluidics-based apparatus.The unique core-shell structured nanoparticle facilitates vaccine uptake and demonstrates a high colloidal stability,and a desirable biodistribution pattern with low liver targeting effect upon intramuscular administration.Extensive evaluations in mice and nonhuman primates revealed strong immunogenicity of SW0123,represented by induction of Th1-polarized T cell responses and high levels of antibodies that were capable of neutralizing not only the wild-type SARS-CoV-2,but also a panel of variants including D614G and N501Y variants.In addition,SW0123 conferred effective protection in both mice and non-human primates upon SARS-CoV-2 challenge.Taken together,SW0123 is a promising vaccine candidate that holds prospects for further evaluation in humans.

关键词： vaccine pattern immunity

Micheliolide exerts effects in myeloproliferative neoplasms through inhibiting STAT3/5 phosphorylation via covalent binding to STAT3/5 proteins

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Blood Science 2023年第4期5卷 258-268页

作者： Huijun Huang Jinqin liu lin Yang Yiru Yan Meng Chen Bing li Zefeng Xu Tiejun Qin shiqiang Qu liang Wang Gang Huang Yue Chen Zhijian Xiao State Key Laboratory of Experimental Hematology National Clinical Research Center for Blood DiseasesHaihe Laboratory of Cell EcosystemInstitute of Hematology&Blood Diseases HospitalChinese Academy of Medical Sciences&Peking Union Medical CollegeTianjin 300020China MDS and MPN Centre Institute of Hematology and Blood Diseases HospitalChinese Academy of Medical Sciences&Peking Union Medical CollegeTianjinChina State Key Laboratory of Medicinal Chemical Biology College of ChemistryNankai University94 Weijin RoadTianjinChina Department of Cell System&Anatomy the University of Texas Health Science Center at San AntonioSan AntonioTX 78229USA Mays Cancer Center Joe R.&Teresa Lozano Long School of Medicinethe University of Texas Health Science Center at San AntonioSan AntonioTX 78229USA Hematologic Pathology Center Institute of Hematology and Blood Diseases HospitalChinese Academy of Medical Sciences&Peking Union Medical CollegeTianjinChina.

Ruxolitinib is a cornerstone of management for some subsets of myeloproliferative neoplasms(MPNs);however,a considerable number of patients respond suboptimally.Here,we evaluated the efficacy of micheliolide(MCL),a natural guaianolide sesquiterpene lactone,alone or in combination with ruxolitinib in samples from patients with MPNs,JAK2V617F-mutated MPN cell lines,and a Jak2V617F knock-in mouse model.MCL effectively suppressed colony formation of hematopoietic progenitors in samples from patients with MPNs and inhibited cell growth and survival of MPN cell lines in vitro.Co-treatment with MCL and ruxolitinib resulted in greater inhibitory effects compared with treatment with ruxolitinib alone.Moreover,dimethylaminomicheliolide(DMAMCL),an orally available derivative of MCL,significantly increased the efficacy of ruxolitinib in reducing splenomegaly and cytokine production in Jak2V617F knock-in mice without evident effects on normal hematopoiesis.Importantly,MCL could target the Jak2V617F clone and reduce mutant allele burden in vivo.Mechanistically,MCL can form a stable covalent bond with cysteine residues of STAT3/5 to suppress their phosphorylation,thus inhibiting JAK/STAT signaling.Overall,these findings suggest that MCL is a promising drug in combination with ruxolitinib in the setting of suboptimal response to ruxolitinib.

关键词： Micheliolide Myeloproliferative neoplasms Ruxolitinib STAT3/5 Suboptimal response

Uniform ordered mesoporous ZnCo2O4 nanospheres for super-sensitive enzyme-free H2O2 biosensing and glucose biofuel cell applications

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Nano Research 2017年第7期10卷 2482-2494页

作者： shiqiang Cui li li Yaping Ding Jiangjiang Zhang QingshengWu Zongqian Hu School of Materials Science and Engineering College of Sciences Shanghai University Shanghai 200444 China State Key Laboratory of Pollution Control and Resource Reuse Tongji University Shanghai 200444 China Beijing Institute of Radiation Medicine Beijing 100850 China

Uniform, ordered mesoporous ZnCo2O4 （meso-ZnCo2O4） nanospheres were successfully synthesized using a sacrificing template method. The meso-ZnCo2O4 nanospheres were used for the first time for H2O2 biosensing and in glucose biofuel cells （GBFCs） as an enzyme mimic. The meso-ZnCo2O4 nanospheres not only exhibited excellent catalytic performance in the H2O2 sensor, achieving a high sensitivity （658.92 μA.mM-1.cm-2） and low detection limit （0.3 nM at signal-to-noise ratio （S/N） = 3）, but also performed as an excellent cathode material in GBFCs, resulting in an open circuit voltage of 0.83 V, maximum power density of 0.32 mW.cm-2, and limiting current density of 1.32 mA.cm-2. The preeminent catalytic abilities to H2O2 and glucose may be associated with the large specific surface area of the mesoporous structure in addition to the intrinsic catalytic activity of ZnCo2O4. These significant findings provide a successful basis for developing methods for the supersensitive detection of H2O2 and enriching catalytic materials for biofuel cells.

关键词： mesoporous ZnCo2O4,H2O2 biosensing,glucose biofuel cells

临床医生走进局部解剖学课堂的教学实践及反馈

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

中国高等医学教育 2024年第2期 70-71页

作者：孙琰琰张士强李治华邵金平帖红艳李鸣臧卫东曹靖郑州大学基础医学院河南郑州450001

基础解剖和临床应用密切联系。低年级医学生学习解剖普遍感到枯燥和抽象。郑州大学人体解剖学系借鉴国外的授课模式,将相关科室临床医生请进解剖学课堂,与人体解剖学系教师共同授课,提高学生的学习兴趣,增加职业认同感。

关键词：局部解剖学临床与基础相结合职业认同感教学改革

维普期刊数据库博看期刊

Cobalt-based hydroxide nanoparticles @ N-doping carbonic frameworks core-shell structures as highly efficient bifunctional electrocatalysts for oxygen evolution and oxygen reduction reactions

在线全文

学校读者我要写书评

暂无评论

Nano Research 2018年第3期11卷 1482-1489页

作者： shiqiang Feng Cheng liu Zhigang Chai Qi li Dongsheng Xu Beijing National Laboratory for Molecular Sciences State Key Laboratory for Structural Chemistry of Unstable and Stable SpeciesCollege of Chemistry and Molecular Engineering Peking University Beijing 100871 China

高度有效、地球丰富的氧进化 / 减小反应(OER/ORR ) 的发展催化剂为可充电的金属空气电池是必要的。此处，基于钴的氢氧化物 nanoparticles @做 N 碳的框架(CoOHCat@NCF ) 核心壳结构作为高度稳定、有效的 OER/ORR bifunctional 催化剂... 详细信息

高度有效、地球丰富的氧进化 / 减小反应(OER/ORR ) 的发展催化剂为可充电的金属空气电池是必要的。此处，基于钴的氢氧化物 nanoparticles @做 N 碳的框架(CoOHCat@NCF ) 核心壳结构作为高度稳定、有效的 OER/ORR bifunctional 催化剂被设计了。获得的合成表演在碱的媒介提高了催化活动和优秀稳定性。在 OER，高周转频率(在 0.36 V 的过电位的 2.03 s -1) ，在高当前的密度的低过电位(100 妈？？

关键词：氢氧化物活动组织框架钴基反应 evolution 金属空气电池进化

Interface regulation of Cu_(2)Se via Cu–Se–C bonding for superior lithium-ion batteries

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Nano Research 2023年第2期16卷 2421-2427页

作者： Kefu Zhu shiqiang Wei Quan Zhou Shuangming Chen Yunxiang lin Pengjun Zhang Yuyang Cao Changda Wang Yixiu Wang Yujian Xia Dengfeng Cao Zeinab Mohamed Xin Guo Xiya Yang Xiaojun Wu li Song National Synchrotron Radiation Laboratory Chinese Academy of Sciences(CAS)Center for Excellence in NanoscienceUniversity of Science and Technology of ChinaHefei 230029China Institutes of Physical Science and Information Technology Anhui UniversityHefei 230601China School of Chemistry and Material Sciences University of Science and Technology of ChinaHefei 230026China

Transition metal selenides have aroused great attention in recent years due to their high theoretical capacity.However,the huge volume fluctuation generated by conversion reaction during the charge/discharge process results in the significant electrochemical performance reduction.Herein,the carbon-regulated copper(I)selenide(Cu_(2)Se@C)is designed to significantly promote the interface stability and ion diffusion for selenide electrodes.The systematic X-ray spectroscopies characterizations and density functional theory(DFT)simulations reveal that the Cu–Se–C bonding forming on the surface of Cu2Se not only improves the electronic conductivity of Cu_(2)Se@C but also retards the volume change during electrochemical cycling,playing a pivotal role in interface regulation.Consequently,the storage kinetics of Cu_(2)Se@C is mainly controlled by the capacitance process diverting from the ion diffusion-controlled process of Cu2Se.When employed this distinctive Cu_(2)Se@C as anode active material in li coin cell configuration,the ultrahigh specific capacity of 810.3 mA·h·g^(−1)at 0.1 A·g^(−1)and the capacity retention of 83%after 1,500 cycles at 5 A·g^(−1)is achieved,implying the best Cu-based li^(+)-storage capacity reported so far.This strategy of heterojunction combined with chemical bonding regulation opens up a potential way for the development of advanced electrodes for battery storage systems.

关键词： Cu–Se–C bonding interface regulation lithium-ion battery X-ray absorption spectroscopy(XAS) operando synchrotron radiation X-ray diffraction(SRXRD)

断指再植术后血管危象的相关危险因素的Logistic回归分析

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

中国医药指南 2024年第11期22卷 23-26页

作者：王金应黄高峰谢钦赐黄林郑俊杰卓世强李泳峰晋江市医院(上海市第六人民医院福建医院)骨科泉州362200 福建省创伤骨科修复重建临床医学研究中心福州362200

目的通过Logistic回归分析明确断指再植后出现血管危象的主要危险因素。方法回顾性分析2021年1月—2023年10月本院收治断指再植总共68例患者的临床资料,按手术结束后有无血管危象归纳成发生组和未发生组,比较两组基本资料及临床资料差异... 详细信息

目的通过Logistic回归分析明确断指再植后出现血管危象的主要危险因素。方法回顾性分析2021年1月—2023年10月本院收治断指再植总共68例患者的临床资料,按手术结束后有无血管危象归纳成发生组和未发生组,比较两组基本资料及临床资料差异,对存在差异的项目进行Logistic回归分析,确定影响术后血管危象发生的主要危险因素。结果68例患者中12例发生血管危象(包含动脉危象7例和静脉危象5例),总发生率是17.65%。旋转撕脱伤、完全离断、末节离断、吸烟史、动脉修复1根、静脉未吻合、就医时间>1.5 h、缺血时间>1.5 h、焦虑为术后发生血管危象的危险因素,OR值均>1。结论旋转撕脱伤、完全离断、末节离断、吸烟史、动脉修复1根、静脉未吻合、就医时间或缺血时间>1.5 h、焦虑是术后血管危象的危险因素,对有以上危险因素者应尽量缩短术前时间,同时术中操作需更细致,尽量达到多根血管修复,对患者加强宣教及心理干预,并警惕吸烟给血管危象带来的影响。

关键词：血管危象断指再植术危险因素预防对策

维普期刊数据库

在线全文

学校读者我要写书评

暂无评论

仿生技术在猪人工授精中的应用研究进展

中国畜禽种业 2024年第6期20卷 44-53页

作者：李芳芳林晓蒋世强黄秉龙梁志敏唐荣福胡传活冯文春广西壮族自治区畜禽品种改良站广西南宁530001 南宁壮博生物科技有限公司广西南宁530004 繁殖生理3.广西大学动物科技学院广西南宁530004 广西壮族自治区扶绥种畜场广西崇左532100

猪人工授精技术是科学养猪的重要手段,对养猪业向规模化、标准化、工业化发展起到重要的促进作用。生产中,仿生技术常应用于公猪的调教、采精、母猪的发情鉴定、输精等环节。该文综述了基于母猪与公猪效应仿生技术下,猪人工授精操作方... 详细信息

猪人工授精技术是科学养猪的重要手段,对养猪业向规模化、标准化、工业化发展起到重要的促进作用。生产中,仿生技术常应用于公猪的调教、采精、母猪的发情鉴定、输精等环节。该文综述了基于母猪与公猪效应仿生技术下,猪人工授精操作方法、配套用品、器械、设备的改良与发展,猪人工授精仿生技术在促进公猪精液质量、母猪发情、繁殖率提高等方面的应用效果,提出仿生技术存在的主要问题及展望。

关键词：猪人工授精仿生技术公猪精液母猪发情鉴定

维普期刊数据库

Single-cell analysis reveals an Angpt4-initiated EPDC-EC-CM cellular coordination cascade during heart regeneration

在线全文

学校读者我要写书评

暂无评论

Protein & Cell 2023年第5期14卷 350-368页

作者： Zekai Wu Yuan Shi Yueli Cui Xin Xing liya Zhang Da liu Yutian Zhang Ji Dong li Jin Meijun Pang Rui-Ping Xiao Zuoyan Zhu Jing-Wei Xiong Xiangjun Tong Yan Zhang shiqiang Wang Fuchou Tang Bo Zhang Key Laboratory of Cell Proliferation and Differentiation of the Ministry of Education Peking University Genome Editing Research CenterCollege of Life SciencesPeking UniversityBeijing 100871China Beijing Advanced Innovation Center for Genomics(ICG) College of Life SciencesPeking UniversityBeijing 100871China State Key Laboratory of Membrane Biology Institute of Molecular MedicineCollege of Future TechnologyPeking UniversityBeijing 100871China State Key Laboratory of Membrane Biology College of Life SciencesPeking UniversityBeijing 100871China Institute of Cardiovascular Sciences and Key Laboratory of Molecular Cardiovascular Sciences School of Basic Medical SciencesMinistry of EducationPeking University Health Science CenterBeijing 100191China Institute of Molecular Medicine College of Future TechnologyPeking UniversityBeijing 100871China

Mammals exhibit limited heart regeneration ability,which can lead to heart failure after myocardial infarction.In contrast,zebrafish exhibit remarkable cardiac regeneration capacity.Several cell types and signaling pathways have been reported to participate in this process.However,a comprehensive analysis of how different cells and signals interact and coordinate to regulate cardiac regeneration is unavailable.We collected major cardiac cell types from zebrafish and performed high-precision single-cell transcriptome analyses during both development and post-injury regeneration.We revealed the cellular heterogeneity as well as the molecular progress of cardiomyocytes during these processes,and identified a subtype of atrial cardiomyocyte exhibiting a stem-like state which may transdifferentiate into ventricular cardiomyocytes during regeneration.Furthermore,we identified a regeneration-induced cell(RIC)population in the epicardium-derived cells(EPDC),and demonstrated Angiopoietin 4(Angpt4)as a specific regulator of heart regeneration.angpt4 expression is specifically and transiently activated in RIC,which initiates a signaling cascade from EPDC to endocardium through the Tie2-MAPK pathway,and further induces activation of cathepsin K in cardiomyocytes through RA signaling.Loss of angpt4 leads to defects in scar tissue resolution and cardiomyocyte proliferation,while overexpression of angpt4 accelerates regeneration.Furthermore,we found that ANGPT4 could enhance proliferation of neonatal rat cardiomyocytes,and promote cardiac repair in mice after myocardial infarction,indicating that the function of Angpt4 is conserved in mammals.Our study provides a mechanistic understanding of heart regeneration at single-cell precision,identifies Angpt4 as a key regulator of cardiomyocyte proliferation and regeneration,and offers a novel therapeutic target for improved recovery after human heart injuries.

关键词： scRNA-seq zebrafish heart regeneration Angpt4 EPDC