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Transcriptomic analysis reveals hub genes and pathways in response to acetic acid stress in Kluyveromyces marxianus during high-temperature ethanol fermentation

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Stress Biology 2023年第1期3卷 288-299页

作者： Yumeng Li shiqi hou Ziwei Ren Shaojie Fu Sunhaoyu Wang Mingpeng Chen Yan Dang Hongshen Li Shizhong Li Pengsong Li Beijing Key Lab for Source Control Technology of Water Pollution College of Environmental Science and EngineeringBeijing Forestry UniversityBeijing 100083China Engineering Research Center for Water Pollution Source Control&Eco-Remediation College of Environmental Science and EngineeringBeijing Forestry UniversityBeijing 100083China Institute of New Energy Technology Tsinghua UniversityBeijing 100084China

The thermotolerant yeast Kluyveromyces marxianus is known for its potential in high-temperature ethanol fermentation,yet it suffers from excess acetic acid production at elevated temperatures,which hinders ethanol *** better understand how the yeast responds to acetic acid stress during high-temperature ethanol fermentation,this study investigated its transcriptomic changes under this *** sequencing(RNA-seq)was used to identify differentially expressed genes(DEGs)and enriched gene ontology(GO)terms and pathways under acetic acid *** results showed that 611 genes were differentially expressed,and GO and pathway enrichment analysis revealed that acetic acid stress promoted protein catabolism but repressed protein synthesis during high-temperature ***-protein interaction(PPI)networks were also constructed based on the interactions between proteins coded by the *** genes and key modules in the PPI networks were identified,providing insight into the mechanisms of this yeast’s response to acetic acid *** findings suggest that the decrease in ethanol production is caused by the imbalance between protein catabolism and protein ***,this study provides valuable insights into the mechanisms of ***’s response to acetic acid stress and highlights the importance of maintaining a proper balance between protein catabolism and protein synthesis for high-temperature ethanol fermentation.

关键词： Kluyveromyces marxianus Acetic acid Transcriptomics Protein-protein interaction network

Neogene integrative stratigraphy,biotas,and paleogeographical evolution of the Qinghai-Tibetan Plateau and its surrounding areas

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Science China Earth Sciences 2024年第4期67卷 1326-1359页

作者： Tao DENG Xiaomin FANG Qiang LI shiqi WANG Feixiang WU Sukuan hou Jiao MA Qigao JIANGZUO Danhui SUN Yan ZHENG Qinqin SHI Boyang SUN Lu LI Key Laboratory of Vertebrate Evolution and Human Origins Institute of Vertebrate Paleontology and PaleoanthropologyChinese Academy of SciencesBeijing 100044China CAS Center for Excellence in Life and Paleoenvironment Beijing 100044China University of Chinese Academy of Sciences Beijing 100049China CAS Center for Excellence in Tibetan Plateau Earth Sciences and Key Laboratory of Continental Collision and Plateau Uplift Institute of Tibetan Plateau ResearchChinese Academy of SciencesBeijing 100101China Key Laboratory of Orogenic Belts and Crustal Evolution School of Earth and Space SciencesPeking UniversityBeijing 100871China

The remarkable uplift of the Tibetan Plateau in the Neogene had great impacts on the climate and environment of East Asia and even the ***,establishment of the Neogene stratigraphic framework of the Tibetan Plateau is of great significance to research in various fields of *** on marine sediments,the international chronostratigraphic system of the Neogene is divided into six stages in the Miocene and two stages in the *** the beginning of the Cenozoic,the share of terrestrial strata on continents has increased *** the Neogene,it had far exceeded that of marine strata,and almost all deposits on the Tibetan Plateau and its surrounding areas were terrestrial *** China,the Miocene includes five stages and the Pliocene includes two *** for the Tunggurian of the Miocene,which has a lower boundary at 15 Ma,the other stages have the same paleomagnetic definitions and time intervals as the corresponding international marine *** fossils play a very important role in the division and correlation of Cenozoic terrestrial strata,and rodent,carnivore,proboscidean,perissodactyl and artiodactyl fossils are especially important in Neogene terrestrial *** are many basins with well-exposed strata and abundant mammalian fossils in the Tibetan *** lower boundary stratotype sections of the Neogene Xiejian and Bahean stages are located respectively in the Xining and Linxia basins,and there are precise paleomagnetic dates in coordination with mammalian *** lower boundary stratotypes of other stages can also be effectively determined in the Tibetan *** first appearing mammalian genera in East Asia also appeared in the Tibetan Plateau and its surrounding areas,especially in the Linxia Basin on the northeast margin and in the Siwaliks on the southwest *** them,Prodeinotherium first appeared at the bottom of the Miocene in the Siwaliks,and the earliest Hipparion of the Old

关键词： Tibetan Plateau Neogene Terrestrial stratum Mammalian fossil Paleomagnetism

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基于知识指导的安全强化学习路由算法

中国工程机械学报 2022年第4期20卷 288-293页

作者：李婧侯诗琪上海电力大学计算机科学与技术学院上海201306

在大流量传输场景中,传统启发式路由选择协议无法根据网络状态动态调整路由策略,而基于数据驱动的路由协议在训练初期无法保证网络吞吐量。针对此问题,提出基于先验知识指导的安全强化学习路由算法,把先验知识引入深度强化学习模型的动... 详细信息

在大流量传输场景中,传统启发式路由选择协议无法根据网络状态动态调整路由策略,而基于数据驱动的路由协议在训练初期无法保证网络吞吐量。针对此问题,提出基于先验知识指导的安全强化学习路由算法,把先验知识引入深度强化学习模型的动作选择,结合ε-greedy策略,根据网络状态对下一跳进行评估和约束,必要时提供更优动作,避免无效动作。基于Keras与Networkx的仿真实验表明:该算法可使网络保持较高的吞吐量,网络性能波动可稳定在较小的范围内,模型收敛速度显著提升。

关键词：先验知识深度强化学习路由选择智能路由吞吐量

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改进HED网络在轴承自动测量中的应用研究

电子测量技术 2024年第2期47卷 142-149页

作者：陈世奇侯明李鹏程北京信息科技大学自动化学院北京100192

针对在工业环境中传统接触式测量轴承尺寸操作复杂且检测成本高的问题,提出一种新的加入注意力机制和结合Canny算法的改进型HED网络边缘检测算法。该方法基于HED网络,将主干网络第4、5个阶段的卷积层替换成连续空洞卷积,并设置网络的第3... 详细信息

针对在工业环境中传统接触式测量轴承尺寸操作复杂且检测成本高的问题,提出一种新的加入注意力机制和结合Canny算法的改进型HED网络边缘检测算法。该方法基于HED网络,将主干网络第4、5个阶段的卷积层替换成连续空洞卷积,并设置网络的第3、4层池化步长为1,增大模型的感受野,提高输出的边缘图像精度;加入高效通道注意力机制ECA模块,有效抑制无关纹理特征和非边缘像素等影响;使用Canny算法中的非极大值抑制和双阈值处理算法,对检测的粗边缘进行细化,得到更加精确的轴承边缘;使用最小二乘圆拟合算法,获取轴承的内外环尺寸参数。实验结果表明,改进后的HED网络在ODS和OIS指标上分别达到了0.811和0.835,该方法可以有效实现轴承边缘检测并保证轴承尺寸测量精度。

关键词： HED网络轴承边缘检测尺寸测量空洞卷积注意力机制

基于层次分析—模糊综合评价法的新旧动能转换绩效评价研究

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山东理工大学学报（社会科学版） 2019年第1期35卷 9-14页

作者：孙秀梅侯士奇山东理工大学管理学院山东淄博255000 山东理工大学经济学院山东淄博255000

设置科学合理的新旧动能转换绩效评价体系有助于推进区域高质量发展。运用层次分析法和模糊综合评价法,从质量效益、创新发展、对外开放和环保民生4个方面选取19个因子,构建新旧动能转换绩效评价指标体系,并将山东省与河北省、浙江省、... 详细信息

设置科学合理的新旧动能转换绩效评价体系有助于推进区域高质量发展。运用层次分析法和模糊综合评价法,从质量效益、创新发展、对外开放和环保民生4个方面选取19个因子,构建新旧动能转换绩效评价指标体系,并将山东省与河北省、浙江省、江苏省等3个东部沿海省份进行新旧动能转换绩效评价比较分析,指出山东省新旧动能转换工作中存在的不足,并提出了改进建议,以期助推山东省新旧动能转换工作取得新成效。

关键词：新旧动能转换绩效评价层次分析法模糊综合评价法

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不同太阳波谱的聚光光伏系统冷却功率研究

东北电力技术 2022年第8期43卷 10-15页

作者：侯诗琪李勇李德洪东华大学环境科学与工程学院上海201620 中国直升机设计研究所天津300308

当前高倍聚光光伏系统(CPV)的发电效率和功率仍受冷却条件限制。为实现光伏电池在低冷却热流密度条件下最大功率输出,提出通过光谱分频技术实现太阳波谱-发电效率-发电功率-冷却功率的匹配,首先基于光伏电池外量子效率,分析了不同太阳... 详细信息

当前高倍聚光光伏系统(CPV)的发电效率和功率仍受冷却条件限制。为实现光伏电池在低冷却热流密度条件下最大功率输出,提出通过光谱分频技术实现太阳波谱-发电效率-发电功率-冷却功率的匹配,首先基于光伏电池外量子效率,分析了不同太阳波谱分频后发电效率和发电功率的变化规律,然后搭建了基于微热管阵列冷却的光谱分频CPV试验系统,初步在400~700 nm和740~940 nm两段波谱进行了试验研究。结果表明,光伏电池在该两段波谱下发电功率相差不大,但400~700 nm波谱的发电效率比740~940 nm波谱小,后者最大接近23%,相比全光谱下的发电效率提高9%;400~700 nm波谱需要冷却功率为740~940 nm波谱的2倍。可见,通过光谱分频技术控制光伏电池接收太阳辐照的波谱段,可以降低CPV系统的冷却功率,能在相同冷却功率条件下实现系统更大的发电功率输出。

关键词：光谱分频 CPV 发电效率发电功率冷却功率

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退化草原草场修复和恢复理论与技术研究进展

生态科学 2024年第05期43卷 207-215页

作者：阳辉廉诗启曹建生侯翔龙中国科学院遗传与发育生物学研究所农业资源研究中心中国科学院农业水资源重点实验室丰宁满族自治县林业和草原局河北省科学院地理科学研究所河北省地理信息开发应用工程技术研究中心

由于对草原生态功能的认识不足,对其生产功能的利用和依赖过大, 90%的草原出现了不同程度的退化,草原生态系统破坏严重,治理速度远跟不上退化速度。综述了国内外有关于退化草原草场修复机制和技术方面的研究进展,梳理和总结过去退化草... 详细信息

由于对草原生态功能的认识不足,对其生产功能的利用和依赖过大, 90%的草原出现了不同程度的退化,草原生态系统破坏严重,治理速度远跟不上退化速度。综述了国内外有关于退化草原草场修复机制和技术方面的研究进展,梳理和总结过去退化草原生态修复工作中的经验和问题。结果表明:在机制研究方面,基于功能性状的群落生态学理论、生态系统下行效应原理、阈值模型及集成过滤模型成为退化草地恢复的最新理论依据,同时,草原生态系统退化不仅包括植被群落盖度发生变化,还包括优势植物物种发生变化,牧草品质和产量下降,物种多样性的流失;在技术研究方面,草地恢复从单项技术改良转向系统综合治理,形成以生物多样性维持、群落结构优化配置、土壤及种子库修复为主体思路的恢复治理技术。目前,比较成熟的草原修复技术包括围栏封育、飞播种草、浅耕翻、免耕补播、划区轮牧和限时放牧等。同时,政府为遏制草地退化制定了一系列的政策,设立了众多生态建设项目,如草原家庭承包制、退耕还林还草、退牧还草、京津风沙源治理工程等。因地制宜、精准施策,集成创新草原生态修复技术体系和生产可持续发展的双赢模式,是未来我国草地退化与恢复的研究方向。

关键词：退化草原生态修复围栏封育以草定畜草畜平衡

Neogene integrative stratigraphy and timescale of China

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Science China Earth Sciences 2019年第1期62卷 310-323页

作者： Tao DENG Sukuan hou shiqi WANG Key Laboratory of Vertebrate Evolution and Human Origins Institute of Vertebrate Paleontology and Paleoanthropology Chinese Academy of Sciences CAS Center for Excellence in Life and Paleoenvironment Department of Earth Sciences University of Chinese Academy of Sciences Department of Earth Sciences Nanjing University

The widely exposed Chinese Neogene terrestrial deposits provide the best circumstance for the establishment of an accurate chronostratigraphic system of Eurasia, and the rapidly evolved mammalian fossils contribute efficiently to the division and correlation of Asian Neogene strata. A uniform Neogene biostratigraphic framework for China has already been established,with seven mammalian ages named. With a developed stratigraphic basis for the geochronologic "ages", seven chronostratigraphic "stage" have been established for the Chinese Neogene terrestrial strata, namely the Miocene Xiejian, Shanwangian,Tunggurian, Bahean, and Baodean stages, and the Pliocene Gaozhuangian and Mazegouan stages. Based on a series of research achievements, refined biostratigraphic, paleomagnetic and isotopic methods were combined and applied to continuous sections,and a Chinese Neogene chronostratigraphic sequence with accurate geological ages was established and improved in recent years. The lower boundaries of most of the stages could be correlated with those of the marine stages in the International Chronostratigraphic Chart, except the Tunggurian Stage, which is correlated with the European land mammal age. The biostratigraphic markers of the Chinese Neogene stages are usually first appearance of a single taxon, some representing regional species replacement, others indicating intercontinental migration of certain taxa. Candidate stratotype sections have been proposed for all the Chinese Neogene stages according to the principle and rule of modern stratigraphy, and other Chinese Neogene strata in different regions are comprehensively correlated.

关键词： Neogene Stage/age Mammal Biostratigraphy Chronostratigraphy Magnetostratigraphy China

A bizarre tandem-horned elasmothere rhino from the Late Miocene of northwestern China and origin of the true elasmothere

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Chinese Science Bulletin 2013年第15期58卷 1811-1817页

作者： DENG Tao WANG shiqi hou SuKuan Key Laboratory of Evolutionary Systematics of Vertebrates Institute of Vertebrate Paleontology and PaleoanthropologyChinese Academy of Sciences Department of Geology Northwest University

Although the modern Indian and Javan rhinos have a single horn on their noses,the extinct one-horned rhino Elasmotherium was a source for the legendary unicorn,because the latter had a very long horn on its forehead and lived with the prehistoric human beings who drew its images on cave *** rhinos first appeared in South Asia in the Early Miocene,but the origin of Elasmotherium has been *** other elasmotheres have a weak or strong nasal horn,whereas Elasmotherium seems to lose the nasal horn of its ancestors and to get a huge frontal horn apparently *** we report the first discovered skull of Sinotherium lagrelii from the Late Miocene red clays in the Linxia Basin,northwestern *** skull has an enormous nasofrontal horn boss shifted posteriorly and a smaller frontal horn boss,which are connected to each other,indicating an intermediate stage for the single frontal horn of *** and phylogenetic analyses confirm that Sinotherium is a transitional taxon between Elasmotherium and other elasmotheres,positioned near the root of the giant unicorn clade and originated in a subarid *** posteriorly shifted nasal horn has a more substantial support and the arched structure of the nasofrontal area is an adaptation for a huge horn.

关键词：中国西北地区晚中新世犀牛原产地串联系统发育分析调制解调器人类居住