检索结果-南通市图书馆

A causal multiomics study discriminates the early immune features of Ad5-vectored Ebola vaccine recipients

The Innovation 2024年第3期5卷 77-87页

作者： Zhe Zhang Wenjing Yang Zhengshan Chen Haoang Chi shipo wu Wanru Zheng Ruochun Jin Busen Wang Yudong Wang Nan Huo Jinlong Zhang Xiaohong Song Liyang Xu Jun Zhang Lihua Hou Wei Chen Institute of Biotechnology Academy of Military Medical SciencesBeijing 100071China Department of Intelligent Data Science College of Computer Science and TechnologyNational University of Defense TechnologyChangsha 410073China Intelligent Game and Decision Lab Academy of Military ScienceBeijing 100091China

The vaccine-induced innate immune response is essential for the generation of an antibody response.To date,how Ad5-vectored vaccines are influenced by preexisting anti-Ad5 antibodies during activation of the early immune response remains unclear.Here,we investigated the specific alterations in GP1,2-specific IgG-related elements of the early immune response at the genetic,molecular,and cellular levels on days 0,1,3,and 7 after Ad5-EBOV vaccination.In a causal multiomics analysis,distinct early immune responses associated with GP1,2-specific IgG were observed in Ad5-EBOV recipients with a low level of preexisting anti-Ad5 antibodies.This study revealed the correlates of the Ad5-EBOV-induced IgG response and provided mechanistic evidence for overcoming preexisting Ad5 immunity during the administration of Ad5-vectored vaccines.

关键词： vaccine immunity alterations

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Lung-Targeted Transgene Expression of Nanocomplexed Ad5 Enhances Immune Response in the Presence of Preexisting Immunity

引用

Engineering 2023年第8期27卷 127-139页

作者： Yilong Yang shipo wu Yudong Wang Fangze Shao Peng Lv Ruihua Li Xiaofan Zhao Jun Zhang Xiaopeng Zhang Jianmin Li Lihua Hou Junjie Xu Wei Chen Beijing Institute of Biotechnology Beijing 100071China

Recombinant adenovirus serotype 5(Ad5)vector has been widely applied in vaccine development targeting infectious diseases,such as Ebola virus disease and coronavirus disease 2019(COVID-19).However,the high prevalence of preexisting anti-vector immunity compromises the immunogenicity of Ad5-based vaccines.Thus,there is a substantial unmet need to minimize preexisting immunity while improving the insert-induced immunity of Ad5 vectors.Herein,we address this need by utilizing biocompatible nanoparticles to modulate Ad5–host interactions.We show that positively charged human serum albumin nanoparticles((+)HSAnp),which are capable of forming a complex with Ad5,significantly increase the transgene expression of Ad5 in both coxsackievirus–adenovirus receptor-positive and-negative cells.Furthermore,in charge-and dose-dependent manners,Ad5/(+)HSAnp complexes achieve robust(up to227-fold higher)and long-term(up to 60 days)transgene expression in the lungs of mice following intranasal instillation.Importantly,in the presence of preexisting anti-Ad5 immunity,complexed Ad5-based Ebola and COVID-19 vaccines significantly enhance antigen-specific humoral response and mucosal immunity.These findings suggest that viral aggregation and charge modification could be leveraged to engineer enhanced viral vectors for vaccines and gene therapies.

关键词： Adenovirus serotype 5 Vaccine Preexisting immunity Nanoparticles Transgene expression

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Comparative characterization of antibody responses induced by Ad5-vectored spike proteins of emerging SARS-CoV-2 VOCs

引用

Signal Transduction and Targeted Therapy 2022年第8期7卷 3126-3138页

作者： Busen Wang Jinghan Xu shipo wu Zhe Zhang Zhenghao Zhao Jun Zhang Ling Fu Xiaodong Zai Yudong Wang Guanying Zhang ZhengShan Chen Yi Chen Hancong Sun Xiaohong Song Jinlong Zhang Lianhui Zhu Lihua Hou Wei Chen Beijing Institute of Biotechnology No.20 Dongdajie StreetFengtai District100071 BeijingChina

Highly divergent SARS-CoV-2 variants have continuously emerged and spread around the world,and updated vaccines and innovative vaccination strategies are urgently needed to address the global SARS-COV2 pandemic.Here,we established a series of Ad5-vectored SARS-CoV-2 variant vaccines encoding multiple spike proteins derived from the Alpha,Beta,Gamma,Epsilon,Kappa,Delta and Omicron lineages and analyzed the antibody immune responses induced by single-dose and prime-boost vaccination strategies against emerging SARS-CoV-2 variants of concern(VOCs).Single-dose vaccination with SARS-CoV-2 variant vaccines tended to elicit the optimal self-matched neutralizing effects,and Ad5-B.1.351 produced more broad-spectrum cross-neutralizing antibodies against diverse variants.In contrast,prime-boost vaccination further strengthened and broadened the neutralizing antibody responses against highly divergent SARS-CoV-2 variants.The heterologous administration of Ad5-B.1.617.2 and Ad5-B.1.429 to Ad5-WT-primed mice resulted in superior antibody responses against most VOCs.In particular,the Omicron spike could only stimulate self-matched neutralizing antibodies with infrequent cross-reactivities to other variants used in single-dose vaccination strategies;moreover,with prime-boost regimens,this vaccine elicited an optimal specific neutralizing antibody response to Omicron,and prompted cross-antibody responses against other VOCs that were very similar to those obtained with Ad5-WT booster.Overall,this study delineated the unique characteristics of antibody responses to the SARS-CoV-2 VOC spikes with the single-dose or prime-boost vaccination strategies and provided insight into the vaccine development of next SARS-CoV-2 VOCs.

关键词： neutral optimal matched

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Broadly neutralizing antibodies against Omicron-included SARS-CoV-2 variants induced by vaccination

引用

Signal Transduction and Targeted Therapy 2022年第5期7卷 1831-1841页

作者： Xiangyang Chi Yingying Guo Guanying Zhang Hancong Sun Jun Zhang Min Li Zhengshan Chen Jin Han Yuanyuan Zhang Xinghai Zhang Pengfei Fan Zhe Zhang Busen Wang Xiaodong Zai Xuelian Han Meng Hao Ting Fang Jinghan Xu shipo wu Yi Chen Yingying Fang Yunzhu Dong Bingjie Sun Jinlong Zhang Jianmin Li Guangyu Zhao Changming Yu Qiang Zhou Wei Chen Institute of Biotechnology Academy of Military Medical SciencesBeijing 100071China Center for Infectious Disease Research Westlake Laboratory of Life Sciences and BiomedicineKey Laboratory of Structural Biology of Zhejiang ProvinceInstitute of BiologyWestlake Institute for Advanced StudySchool of Life SciencesWestlake UniversityHangzhou 310024 Zhejiang ProvinceChina State Key Laboratory of Pathogen and Biosecurity Institute of Microbiology and EpidemiologyAcademy of Military Medical SciencesBeijing 100071China wuhan Institute of Virology Center for Biosafety Mega-ScienceChinese Academy of SciencesWuhanHubei 430071China

The SARS-CoV-2 Omicron variant shows substantial resistance to neutralization by infection-and vaccination-induced antibodies,highlighting the demands for research on the continuing discovery of broadly neutralizing antibodies(bnAbs).Here,we developed a panel of bnAbs against Omicron and other variants of concern(VOCs)elicited by vaccination of adenovirus-vectored COVID-19 vaccine(Ad5-nCoV).

关键词： neutral vaccination Omi

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

重组人神经生长因子通过抑制小胶质细胞炎症反应发挥神经保护作用

引用

中国药理学与毒理学杂志 2023年第8期37卷 574-582页

作者：李瑶陈旖朱丹妮宋小红张金龙张哲赵拯浩侯利华吴诗坡陈薇军事科学院军事医学研究院生物工程研究所北京100071

目的探讨重组人神经生长因子(rhNGF)的神经保护作用及其机制。方法BV2小胶质细胞分为细胞对照组、脂多糖(LPS)组及LPS+rhNGF 50和500μg·L^(-1)组。除细胞对照组外,其余3组用LPS 1 mg·L^(-1)刺激BV2细胞24 h诱导BV2细胞炎症反应,随后L... 详细信息

目的探讨重组人神经生长因子(rhNGF)的神经保护作用及其机制。方法BV2小胶质细胞分为细胞对照组、脂多糖(LPS)组及LPS+rhNGF 50和500μg·L^(-1)组。除细胞对照组外,其余3组用LPS 1 mg·L^(-1)刺激BV2细胞24 h诱导BV2细胞炎症反应,随后LPS+rhNGF组加rhNGF继续孵育48 h。孵育结束后收集BV2细胞,并收集各组培养上清即条件培养基(MCM),各组BV2细胞和MCM分别与小鼠脑神经瘤细胞Neuro-2a(N2a)共培养12或24 h。CCK-8法检测BV2细胞存活率,实时荧光定量PCR检测BV2细胞中促炎因子白细胞介素(IL-6)、肿瘤坏死因子α(TNF-α)和IL-1β及抗炎因子IL-4和IL-10 mRNA表达水平,CBA法检测BV2细胞培养上清中IL-6,TNF-α,IL-4和IL-10浓度,免疫荧光法检测BV2细胞M1表型标志物CD16和M2表型标志物CD206表达,NeuN/TUNEL共染法和AnnexinⅤ/7-AAD法检测N2a细胞凋亡率。结果与细胞对照组相比,LPS组BV2细胞存活率显著下降(P<0.01),细胞变圆且胞体变大;促炎因子IL-1β,IL-6和TNF-α及抗炎因子IL-10 mRNA水平显著升高(P<0.01),抗炎因子IL-4 mRNA水平下降(P<0.01);IL-6和TNF-α蛋白水平显著升高(P<0.01),IL-4蛋白水平无显著差异;CD16和CD206阳性细胞百分比明显升高(P<0.01),CD16阳性细胞升高更加明显;与BV2细胞或其MCM共培养的N2a细胞存活率显著降低且凋亡率显著升高(P<0.05,P<0.01)。与LPS组相比,LPS+rhNGF 50和500μg·L^(-1)组BV2细胞存活率显著提高(P<0.01);IL-1β,IL-6和TNF-αmRNA水平显著降低(P<0.05),IL-4和IL-10 mRNA水平显著提高(P<0.05,P<0.01);IL-6和TNF-α蛋白水平显著降低(P<0.05,P<0.01),IL-4蛋白水平无显著差异,IL-10浓度低于检测限;CD206阳性细胞百分比显著升高(P<0.01),CD16阳性细胞百分比显著降低(P<0.05)。与LPS处理的BV2细胞或MCM-LPS组相比,与rhNGF 50和500μg·L^(-1)处理的BV2细胞共培养组及与MCM-rhNGF 50和500μg·L^(-1)共培养组N2a细胞存活率显著升高且凋亡率显著降低(P<0.05,P<0.01)。结论rhNGF可通过抑制BV2细胞炎症反应诱导BV2细胞向M2表型转化,抑制N2a细胞凋亡,从而发挥神经保护作用。

关键词：神经炎症重组人神经生长因子小胶质细胞炎症反应细胞凋亡

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

蓖麻油基润滑油生物降解实验方法的改进

引用

天津师范大学学报（自然科学版） 2008年第4期28卷 13-15页

作者：吴新世吴诗坡叶启军王茜刁虎欣叶锋天津理工大学生物工程系天津300191 南开大学生命科学学院天津300071 南开大学蓖麻工程研究中心天津300071

通过研究蓖麻油基新型润滑油的可降解性,确立了一种快速有效降解蓖麻油基润滑油的3组瓶即油样瓶、毒性瓶和中性瓶的实验方法,与常规实验方法比较,该新型实验方法利用筛选的最佳菌种1B-21混合菌株,在最适降解条件下,得到该润滑油的降解率... 详细信息

通过研究蓖麻油基新型润滑油的可降解性,确立了一种快速有效降解蓖麻油基润滑油的3组瓶即油样瓶、毒性瓶和中性瓶的实验方法,与常规实验方法比较,该新型实验方法利用筛选的最佳菌种1B-21混合菌株,在最适降解条件下,得到该润滑油的降解率为82.02%,比常规实验方法(70.20%)约高10.78%,而且利用该方法检测,结果准确可靠、重复性好.

关键词：蓖麻油基润滑油生物降解性评价分析方法

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：