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Susceptibility of Aedes flavopictus miyarai and Aedes galloisi mosquito species in Japan to dengue type 2 virus

Asian Pacific Journal of Tropical Biomedicine 2016年第5期6卷 446-450页

作者： Raweewan Srisawat Thipruethai Phanitchat Narumon Komalamisra Naoki Tamori Lucky Runtuwene Kaori Noguchi Kyoko Hayashida shinya hidano Naganori Kamiyama Ikuo Takashima Tomohiko Takasaki Ichiro Kurae Narihiro Narita Takashi Kobayashi Yuki Eshita Faculty of Tropical Medicine Mahidol University Faculty of Medicine Oita University Laboratory of Public Health Department of Environmental Veterinary SciencesGraduate School of Veterinary MedicineHokkaido University Department of Virology 1 National Institute of Infectious Diseases Cultural Anthropology Laboratory Department of Arts and SciencesOhkagakuen University

Objective: To evaluate the potential of local mosquitoes to act as vectors for dengue transmission in ***: Serotype 2 Th NH28/93 was used to test the dengue susceptibility profiles of Aedes flavopictus miyarai(Ae. f. miyarai), Aedes galloisi(Ae. galloisi) and Aedes albopictus(***), which were collected in Japan. We used Aedes aegypti from Thailand as a positive control. The mosquitoes were infected with the virus intrathoracically or orally. At 10 or 14 days post infection, the mosquitoes were dissected and total RNA was extracted from their abdomens, thoraxes, heads and legs. Mosquito susceptibility to dengue virus was evaluated using RT-PCR with dengue virus-specific primers. Differences in the infection and mortality rates of the different mosquito species were tested using Fisher's exact probability ***: The infection rates for dengue virus administered intrathoracically to Ae. f. miyarai,Ae. galloisi and Aedes aegypti mosquitoes were identical by RT-PCR on Day 10 post *** of the body parts we tested were RT-PCR-positive for dengue virus. For the orally administered virus, the infection rates in the different body parts of the Ae. f. miyarai mosquitoes were slightly higher than those of Ae. albopictus mosquitoes, but were similar to the control mosquitoes(P > 0.05). The mortality rates for Ae. f. miyarai and Ae. albopictus mosquitoes were similar(P = 0.19). Our data indicated that dengue virus was able to replicate and disseminate to secondary infection sites in all of the four mosquito species(Japanese and Thai).Conclusions: Ae. albopictus is a well-known candidate for dengue transmission in Japan. However, our data suggest that Ae. f. miyarai from Ishigaki Island(near Okinawa Island) and Ae. galloisi from Hokkaido(Northern Japan) should also be regarded as potential vectors for dengue transmission in these regions. Further studies on these mosquitoes should be conducted.

关键词： Aedes flavopictus miyarai Aedes galloisi Aedes albopictus Aedes aegypti Dengue type 2 virus Japan Oral infection Intrathoracic inoculation

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