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基于CiteSpace的m6A甲基化研究的文献可视化分析

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中国现代应用药学 2023年第1期40卷 57-67页

作者：黄金梅朱素梅覃淼莫钧茹覃仕娜黎芳梁健钦奉建芳广西中医药大学南宁530200 广西优势中成药与民族药开发工程技术研究中心南宁530200 广西中医药大学中药制剂共性技术研发重点实验室南宁530200

目的分析近年m6A甲基化研究的发展状况、研究热点与前沿趋势。方法通过CNKI和Web of Science数据库检索2013—2021年m6A甲基化相关研究的文献,运用CiteSpace 5.8.R3软件对文献作者进行合作网络分析,对关键词进行共现、聚类和突现分析,... 详细信息

目的分析近年m6A甲基化研究的发展状况、研究热点与前沿趋势。方法通过CNKI和Web of Science数据库检索2013—2021年m6A甲基化相关研究的文献,运用CiteSpace 5.8.R3软件对文献作者进行合作网络分析,对关键词进行共现、聚类和突现分析,绘制知识可视化图谱。结果共获得中文文献438篇、英文文献897篇,年度发文量呈快速增长趋势,高水平期刊主要集中在英文文献上,高产机构有南京医科大学、浙江大学、中山大学等高校。m6A甲基化研究高产作者主要有Chuan He、谷仕艳等,但尚未形成稳定的核心团队。关键词分析显示,m6A甲基化研究内容主要集中在检测技术、调控机制及靶向药物、预后风险模型等方面。结论m6A甲基化研究正处于高速发展时期,其测序技术、不同调控因子的分布状态与疾病发生发展之间的关联性等方向成为主要研究热点。未来,恶性肿瘤、肝细胞癌、胃癌、结直肠癌等疾病的m6A甲基化修饰相关性研究是主要方向。

关键词： m6A CiteSpace 可视化分析文献

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Involvement of the PI3K pathway in Largemouth Bass Virus-induced apoptosis and viral replication

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Involvement of the PI3K pathway in Largemouth Bass Virus-ind...

Ubiquitin-proteasome system is required for efficient replication of Singapore grouper iridovirus

中国水产学会鱼病专业委员会2013年学术研讨会

作者： Xiaohong Huang Wei Wang Youhua Huang Liwen Xu shina Wei Qiwei qin Key Laboratory of Marine Bio-resources Sustainable Utilization South China Sea Institute of OceanologyChinese Academy of Sciences South China Sea Fisheries Research Institute Chinese Academy of Fishery Sciences

Largemouth Bass Micropterus salmoides is an economically important freshwater fishcultured in *** new emerging viral diseases evoked by iridovirus and rhabdovirus havecaused great economic losses in *** better exploring the pathogenesis of LargemouthBass virus(LMBV),a ranavirus isolate,we investigated the underlying mechanism involved inviral replication and virus induced cell death in *** fluorescence microscopy and flowcytometry,we found that LMBV infection in EPC cells induced typical apoptosis,indicated as theappearance of apoptotic bodies,cytochrome C release and MMP ***,caspaseactivity detection revealed caspase-3,8 and 9 were activated with the infection time,suggestedthat not only mitochondrion-mediated,but also death receptor-mediated apoptosis were involvedin LMBV *** treatment with different inhibitors,viral gene transcription and virusproduction were significantly suppressed by the disruption of proteasome and PI3K ***,both proteasome inhibitor MG132 and PI3K inhibitor LY294002 enhanced virusinduced *** addition,LY294002 also affected the cell cycle alteration induced by ***,we speculated that proteasome system and PI3K pathway played crucial rolesin virus replication by regulated apoptosis evoked by LMBV *** together,our resultsprovided new insights into the molecular mechanism of ranavirus infection.

关键词： Largemouth Bass LMBV apoptosis proteasome PI3K pathway virus replication

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Ubiquitin-proteasome system is required for efficient replic...

Molecular cloning and characterization of two types of IκBα homolog in orange-spotted grouper, Epinephelus coioides

中国水产学会鱼病专业委员会2013年学术研讨会

作者： Youhua Huang Xiaohong Huang Zhengliang OuYang Jia Cai shina Wei Ren Gao Qiwei qin Key Laboratory of Marine Bio-resources Sustainable Utilization South China Sea Institute of OceanologyChinese Academy of Sciences State Key Laboratory Breeding Base for Sustainable Exploitation of Tropical Biotic Resources College of Marine ScienceHainan University

Ubiquitin-proteasome system not only played crucial roles in a variety of fundamentalcellular processes,but also was potentially involved in virus replication at different stage of viruslife ***,little information was disclosed on the underlying mechanism by which theubiquitin-proteasome system regulated fish virus *** grouper iridoviurs(SGIV),a large DNA virus which belonged to genus Ranvirus,family Iridovirade,always caused greateconomic losses in grouper *** this study,the roles of ubiquitin-proteasome system inSGIV replication were *** demonstrated that different proteasome inhibitors,including MG132,lactacystin,and bortezomib,significantly reduced virus production,anddelayed SGIV induced cytopathic effect(CPE)progress and cell *** inhibition effect ofMG132 on SGIV replication mainly occurred at the early stage without affecting *** microscopy and electron microscopic observations showed thatMG132 strongly inhibited the formation of virus assembly sites,accompanied by the reduction ofviral gene transcription,protein synthesis and the transportation of viral structural ***,MG132 treatment altered the cell cycle induced by SGIV infection,and significantlysuppressed two transcription factors NF-κB and p53 activity evoked by *** addition,theaddition of an ubiquitin-activating enzyme(E1)inhibitor also showed a significant inhibition onvirus production,and the overexpression of grouper ubiquitin could partially counter theinhibitory effects of *** together,our results presented new insight into the roles ofubiquitin-proteasome system in fish virus infection wherein it was required for SGIV replicationthrough regulating the cell cycle induced by SGIV.

关键词： Iridovirus SGIV Viral replication Virus assembly Ubiquitin-proteasome system,cell cycle

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Molecular cloning and characterization of two types of IκB...

立方Mg_(3)P_(2)弹性性质的理论计算研究

中国水产学会鱼病专业委员会2013年学术研讨会

作者： Ren Gao Youhua Huang Liya Guan shina Wei Xiaohong Huang Yongcan Zhou Qiwei qin State Key Laboratory Breeding Base for Sustainable Exploitation of Tropical Biotic Resources College of Marine Science Hainan University Key Laboratory of Tropical Marine Bio-resources and Ecology South China Sea Institute of OceanologyChinese Academy of Sciences

IκBa is a member of the primary regulators of NF-κB,which can inhibit NF-κB activityby blocking the NF-κB in an inactive state in the *** this study,two types of IκBαhomolog,EcIκBα1and EcIκBα2,were cloned and characterized from orange-spotted grouper,Epinephelus *** complete length of EcIκBα1cDNA is 1629 bp and encodes a putativeprotein of 318 amino acids sharing 85%identity to IκBαof Takifugu rubripes,while the fulllength of EcIκBα2cDNA is 1441 bp,which encodes a protein of 308 residues with 89%identity toIκBαof Siniperca *** with the total 4092 bp genomic DNA of EcIκBα1,EcIκBα2is only 2419 bp *** EcIκBα1and EcIκBα2consist of 6 exons and 5 introns,which is similarto IκBαof Takifugu rubripes and Siniperca *** acid sequence analysis showed thatboth EcIκBα1and EcIκBα2protein contained a same conserved degradation motif DSGLDS intheir N-terminal region and a PEST sequence in the C-terminal region ***κBα1hadsix ankyrin repeats,while EcIκBα2had five ankyrin repeats and another PEST domain whichcontains a NES sequence in ***-PCR revealed that the transcripts of EcIκBα1andEcIκBα2were abundant in spleen but less in skin and ***-luciferase reporter assaysuggested that both EcIκBα1and EcIκBα2were able to inhibit EcC-Rel NF-κB and Ecp65 NF-κBtranscriptional activity in EAGS *** localization analysis showed that EcIκBα1wasdistributed predominantly in the cytoplasm,while EcIκBα2was distributed throughout both thenucleus and the *** expression level of EcIκBα1and EcIκBα2in cells stimulated bylipopolysaccharide(LPS),Vibrio alginolyticus and SGIV revealed that EcIκBα2might be moreeasily to be synthesized and degradated than EcIκBα1during pathogen *** findingssuggested that both EcIκBα1and EcIκBα2involved in grouper immune defense against invasion ofviral and bacterial pathogens by regulating the EcC-Rel NF-κB and Ecp65 NF-κB,and EcIκBα2might play a more significant role d

关键词： Epinephelus coioides EcIκBα 1 and EcIκBα 2 inhibitory activity fish immunity

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功能材料 2023年第3期54卷 3099-3105页

作者：秦祯鑫师小波李世娜山西大学理论物理研究所太原030006 量子光学与光量子器件国家重点实验室太原030006 河北建材职业技术学院信息工程系河北秦皇岛066004

在密度泛函理论(DFT)的基础上,通过第一性原理计算方法在理论上研究了磷化镁(Mg_(3)P_(2))立方Ia3相的弹性性质。运用应变能-应变(E-S)和应力-应变(S-S)两种计算方法获得了立方Ia3 Mg_(3)P_(2)的单晶独立弹性常数和弹性柔顺常数。S-S方... 详细信息

在密度泛函理论(DFT)的基础上,通过第一性原理计算方法在理论上研究了磷化镁(Mg_(3)P_(2))立方Ia3相的弹性性质。运用应变能-应变(E-S)和应力-应变(S-S)两种计算方法获得了立方Ia3 Mg_(3)P_(2)的单晶独立弹性常数和弹性柔顺常数。S-S方法与文献值吻合更好,在此方法的基础上获得了其多晶弹性模量,如弹性模量E、剪切模量G等,还获得了B/G、泊松比、各向异性因子等一系列物理参数。表明立方Ia3 Mg_(3)P_(2)是处于力学稳定状态,同时反映出其具有韧性的特点。表示弹性性质对晶体方向依赖性的杨氏模量及线性压缩系数等的三维曲面图像直观反映出立方I a3 Mg_(3)P_(2)的各向异性程度较弱与各向异性因子反映出的信息一致。此外,计算了Ia3 Mg_(3)P_(2)的德拜温度及不同方向上的声速,结果给出Ia3 Mg_(3)P_(2)原子间作用力弱,且在零温零压下,纵波沿[111]方向传播最快,横波沿[110]方向最慢。

关键词：磷化镁第一性原理晶体结构弹性常数各向异性

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鱼类溶菌酶和组织蛋白酶研究进展

广西科学 2018年第1期25卷 32-35页

作者：魏世娜秦启伟华南农业大学海洋学院，广东广州 510642

溶菌酶和组织蛋白酶在鱼类先天性免疫系统中发挥重要作用。溶菌酶是一种具有生物活性的、能水解粘多糖的无毒、无公害小分子碱性酶,参与机体多种免疫反应,在抵抗外来病原细菌、真菌、病毒或肿瘤入侵中起重要作用。组织蛋白酶是半胱氨酸... 详细信息

溶菌酶和组织蛋白酶在鱼类先天性免疫系统中发挥重要作用。溶菌酶是一种具有生物活性的、能水解粘多糖的无毒、无公害小分子碱性酶,参与机体多种免疫反应,在抵抗外来病原细菌、真菌、病毒或肿瘤入侵中起重要作用。组织蛋白酶是半胱氨酸蛋白酶家族的主要成员,参与机体的多种生理和病理活动,目前在细胞凋亡中的作用日益受到重视。本综述主要介绍近年来鱼类溶菌酶和组织蛋白酶在生物学功能、基因克隆、组织分布及重组蛋白功能活性的新研究进展。

关键词：鱼类溶菌酶组织蛋白酶生物学特性基因与蛋白

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武汉市围生期孕妇母婴健康素养现状及影响因素分析

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循证护理 2022年第8期8卷 1125-1128页

作者：龚诗娜傅玲吴青青万芹华中科技大学同济医学院附属同济医院湖北430024

目的:了解武汉市围生期孕妇母婴健康素养水平现状,分析其影响因素,为提高围生期孕妇母婴健康素养水平提供参考依据。方法:于2021年1月1日—6月1日随机抽取武汉市6所综合性三级甲等医院的720例门诊围生期孕妇作为研究对象,发放一般资料... 详细信息

目的:了解武汉市围生期孕妇母婴健康素养水平现状,分析其影响因素,为提高围生期孕妇母婴健康素养水平提供参考依据。方法:于2021年1月1日—6月1日随机抽取武汉市6所综合性三级甲等医院的720例门诊围生期孕妇作为研究对象,发放一般资料问卷、母婴健康素养测评问卷进行调查。结果:围生期孕妇母婴健康素养总分为(49.82±12.90)分,合格率为15.42%;孕周(OR=1.596)及文化程度(OR=1.704)对围生期孕妇母婴健康素养有影响。结论:武汉市围生期孕妇母婴健康素养较低,合格率较低,怀孕周次及文化程度是围生期孕妇母婴健康素养的影响因素。应当加强对围生期孕妇母婴健康相关知识及技能的健康教育,为促进母婴健康打好扎实基础。

关键词：围生期孕妇健康素养健康教育护理

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