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Microwave Assisted Extraction,Antioxidant Potential and Chromatographic Studies of Some Rasayana Drugs

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Chinese Journal of Integrative Medicine 2015年第7期21卷 523-529页

作者： Ashish Mishra shilpi Mishra shilpi Bhargav CS.bhargava Mayank Thakur Advance Institute of Biotech&Paramedical Sciences Institute for Laboratory Medicine Clinical Chemistry and Pathobiochemistry Charite University of MedicineCampus Benjamin Franklin

Objective： To study and compare the conventional extraction procedure with microwave assisted extraction （MAE） for some Ayurvedic Rasayana drugs and to evaluate their antioxidant potential and carry out the characterization of extracts by thin layer chromatography. Methods： Three Ayurvedic rasayana plants Allium sativum Linn., Bombax ceiba Linn. and Inula racemosa Hook. were evaluated for an improved MAE methodology by determining the effects of grinding degree, extraction solvent, effect of dielectric constant and duration of time on the extractive value. Antioxidant potential of all three drugs was evaluated with 2,2-diphenyl-l-picrylhydrazyl （DPPH） radical scavenging activity and reducing power was determined by using Gallic acid as standard. Further thin layer chromatographic （TLC） analysis was performed on pre-activated Silica Gel G plates and Rf value were compared with those reported for the important biomarkers. Results： The total extractive value for Allium sativum Linn. was 36.95% （w/w） and 49.95% （w/w） for ethanol extraction respectively. In case of Bombax ceiba Linn. the yield of aqueous extract by MAE was 50% （w/w） compared to 42% （w/w） in ethanol （50% v/v）. Percent yield of Inula racernosa Hook. in aqueous extract was found to be 27.55% （w/w） which was better than ethanol extract （50%） where the yield was 25.95% （w/w）. Upon antioxidant activity evaluation A. safivum extract showed an absorbance of 0.980 ± 0.92 at concentration of 500 μg with maximum reducing capacity. This was followed by B. ceiba Linn. 0.825 ± 0.98 and I. racemosa Hook. with 0.799 ± 2.01 at a concentration of 500 μ g. TLC based standardization of A. sativum Linn. extract shows single spot with Rf value of 0.38, B. ceiba Linn. extract shows Rf values were 0.23, 0.58, 0.77, 0.92 and I. racemosa Hook. extract spot had a Rf value of 0.72. Conclusions： A significant improvement in extractive values was observed as a factor of time and other advantages by using MAE technology. All three dru

关键词： antioxidant potential Ayurvedic Rasayana Allinm sativum Linn. Bombax ceiba Inula racemosa,microwave assisted extraction

Bibliometric analysis of Blockchain in the healthcare domain

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Intelligent and Converged Networks 2023年第4期4卷 305-312页

作者： shilpi Garg Rajesh Kumar Kaushal Naveen Kumar Anshul Verma Chitkara Institute of Engineering and Technology Chitkara UniversityPunjab 140401India Institute of Science Banaras Hindu UniversityVaranasi 221005India

As an innovation,Blockchain has transformed numerous industries and sparked the interest of the research community due to its abundance of benefits,opening up diverse research routes in the healthcare sector in the last *** Health 4.0 becoming ubiquitous in the healthcare industry,end-user transactions are being carried out on a decentralized network,making Blockchain profitable to meet the demands of the modern healthcare ***,a detailed analysis of Blockchain is very *** study emphasizes the evolution of science and the preliminary research of Blockchain in healthcare through bibliometric *** the data are extracted from the Scopus database,and the VOSviewer tool is used for analysis.A total of 1152 Scopus articles published between 2018 and 2022 are *** reveal that in 2022,the field of Blockchain experienced a notable increment in the number of publications and a significant growth *** Access became well known in this field and had a large number of *** is observed that China and India are the leading countries in terms of publications on *** study offers a number of recommendations that amateur and professional researchers can use as a benchmark before commencing a Blockchain investigation in the future.

关键词： Blockchain bibliometric healthcare VOSviewer

Maintaining the artemisinin content through direct and indirect in vitro regeneration and their assessment of variations with the field grown mother plants of Artemisia annua L.

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Natural Products and Bioprospecting 2012年第5期2卷 194-199页

作者： shilpi PAUL Madan M GUPTA Suman P S KHANUJA Central Institute of Medicinal and Aromatic Plants(CSIR) LucknowIndia

An efficient protocol for maintaining the artemisinin content in tissue culture and high frequency of in vitro direct and indirect regenerations of multiple shoots of high artemisinin yielding genotypes of Artemisia annua has been developed and their comparison with field grown mother plant has been carried *** eleven elite genotypes(containing more than 1%artemisinin)were tested on seven different modified media *** half MS(Murashige and Skoog’s)media containing 100 mg L^(-1) myo-inositol,0.5 g L^(-1) casine hydrolysate,5 mg L^(-1) biotin,2 mL L^(-1) RT(Revised Tobacco)vitamin stock,0.5 mg L^(-1) BAP and 0.01 mg L^(-1) NAA showed best regeneration while,above modified MS medium containing 0.2 mg L^(-1) BAP and 0.2 mg L^(-1) NAA showed maximum shoot multiplication with maintained artemisinin *** on the chemical profiles of both the systems,minor difference was observed in their artemisinin content.A large scale culture of these plants maintained the normal growth index along with the artemisinin content and could be a better alternative to maintain the high artemisinin yielding genotypes with their genetic constraint in specific media combinations and also used as base material for further genetic improvement.

关键词： direct and indirect regeneration Artemisia annua artemisinin BAP NAA

Interdependence between electrical and magnetic properties of polycrystalline cobalt-substituted tungsten bronze multiferroic ceramics

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Journal of Advanced Dielectrics 2018年第1期8卷 17-24页

作者： shilpi Jindal Sheela Devi Ajay Vasishth Khalid Mujasam Batoo Gagan Kumar Department of Physics Chandigarh University MohaliPunjabIndia Department of Applied Sciences MSITC-4 JanakpuriNew DelhiIndia Department of Applied Sciences Chandigarh Engineering College MohaliPunjabIndia King Abdullah Institute for Nanotechnology King Saud University P.O.Box 2455 Riyadh 11451Saudi Arabia

Polycrystalline cobalt-substituted tungsten bronze ferroelectric ceramics with chemical composition Ba_(5)CaTi_(2-x)Co_(X)Nb_(8)O_(30)(x=0.00,0.02,0.04 and 0.08)were synthesized by solid state reaction technique.X-ray diffraction(XRD)technique was used to confirm the phase formation and it revealed the formation of single phase tetragonal structure with space group *** surface morphology of the samples was studied by using the scanning electron microscopy(SEM)*** dielectric properties such as dielectric constant and dielectric loss have been investigated as a function of temperature and *** P-E and M-H studies confirmed the coexistent of ferroelectricity and magnetism at room *** P-E loop study indicated an increase in the coercive field while the M-H study depicted a decrease in the magnetization with the incorporation of cobalt ions.

关键词： Tungsten bronze solid state reaction technique:electrical properties magnetic properties

Challenges in managing ST elevation myocardial infarction during the COVID-19 pandemic

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World Journal of Cardiology 2021年第4期13卷 76-81页

作者： Megan Smith Aniruddha Singh Douglas McElroy shilpi Mittal Richard Pham Department ofCardiology University of Kentucky College of MedicineBowling GreenKY 42101United States Department of Cardiology Western Kentucky Heart and Lung Research FoundationBowling GreenKY 42101United States Department of Cardiology The Medical CenterBowling GreenKY 42101United States Department of Biology Western Kentucky UniversityBowling GreenKY 42101United States Department of Neurology Vanderbilt University Medical CenterNashvilleTN 37232United States

BACKGROUND Coronavirus disease 2019(COVID-19)may contribute to delayed presentations of acute myocardial *** presentation with late reperfusion is often associated with an increased risk of mechanical complications and adverse *** delays are possible as every patient who is acutely sick is being considered a potential case or a career of ***,standardized personal protective equipment precautions are established for all members of the team,regardless of pending COVID-19 testing which might further add to *** To compare performance measures and clinical outcomes of all patients who presented to our facility with ST elevation myocardial infarction(STEMI)during the COVID-19 pandemic to same time cohort from *** All patients who presented to our facility with STEMI during the pandemic were compared to a matched cohort during the same time period in *** with unknown time of symptom onset and inpatient STEMI patients were *** outcome was major adverse cardiac events(MACE)in-hospital and up to 14 d after STEMI,including death,myocardial infarction,cardiac arrest,or *** differences among groups for continuous variables were tested through ANOVA,using SYSTAT,version ***-square tests of association were used to compare patient characteristics among groups using *** risk scores and associated tests for significance were calculated for discrete variables using MedCalc(MedCalc Software,Ostend,Belgium).RESULTS There was a significantly longer time interval from symptom onset to first medical contact(FMC)in the COVID-19 group(P50%)lesion(s)remote from the culprit ***-hospital and 14 d MACE were more prevalent in the COVID-19 group

关键词： COVID-19 ST elevation myocardial infarction First medical contact to balloon Major adverse cardiac events Cardiac arrest Death

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藜麦生产与应用致谢

藜麦生产与应用致谢

Protein Mediated Silica Particles with pH Controlled Porosity and Morphology

作者： Atul bhargava shilpi Srivastava

首先,作者对世界各地的藜麦研究人员表示衷心感谢,是他们宝贵的工作让我们能够完成关于藜麦这个优良作物的著作。非常感谢Francisco Fuentes博士对本书编写做出的贡献,他在多方面全力支持我们,并与Enrique Martinez博士和Didier Bazile...

首先,作者对世界各地的藜麦研究人员表示衷心感谢,是他们宝贵的工作让我们能够完成关于藜麦这个优良作物的著作。非常感谢Francisco Fuentes博士对本书编写做出的贡献,他在多方面全力支持我们,并与Enrique Martinez博士和Didier Bazile博士一起为本书提供了很多精致图片。同时,对Angel Muiica博士、Andres Zurita-Silva博士、Pablo Olgufn M表示衷心感谢。感谢上述研究人员同意我们分享他们宝贵的研究成果。

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Advances in Microbiology 2016年第14期6卷 986-998页

作者： shilpi Show Brajadulal Chattopadhyay Department of Physics Jadavpur University Kolkata India

Background: The silica leaching activity of some of the mystifying non-pathogenic BKH1 bacteria present in the cluster of hot springs (temperatures range 35°C - 80°C) at Bakreshwar (West Bengal, India, 23°52'48"N;87°22'40"N) has provided some significant advancements in the field of nanotechnology. The present investigation was designed to synthesis the silica particles using bioremediase protein at different pH conditions. Methods: A secretary bacterial protein bioremediase (UniProt Knowledgebase Accession Number P86277) isolated from a thermophilic non-pathogenic bacterium BKH1 (GenBank Accession No. FJ177512) has been used to synthesis the silica particles at different pH conditions (pH at 3.0, 5.0, 8.0, 10.0, and 12.0 respectively). The silica particles were synthesized by the action of bioremediase protein on Tetra-ethyl-orthosilicate (TEOS) under ambient condition. Morphological and compositional studies of the biosynthesized silica particles were characterized by Field emission scanning electron microscope (FE-SEM) equipped with Energy dispersive X-ray analyser (EDX). Results: The Fourier transformed infra-red (FTIR) spectroscopic analysis confirmed the nature as well as occurrence of several functional groups surrounded on the silica particles. The amorphous nature of the prepared silica particles was confirmed by X-ray diffractometer (XRD) study. The Zeta potential (ζ) study revealed the stability of silica particles in neutral pH environment. The Brunauer-Emmett-Teller (BET) surface area measurement confirmed the porosity variation in all biosynthesized silica particles prepared at different pH conditions. Raman spectra analytically depend on their respective specific surface (BET) area. Thermogravimetry tool was used to monitor the effects of the thermal treatment on the surface properties of all the samples. Conclusions: The method for the synthesis of silica particles at different pH condition using the protein bioremediase has a special implication as

关键词： Bioremediase Protein Tetraethyl Orthosilicate Green Chemistry Silica Particles Porosity

Morphological Changes and Antioxidant Activity of <i>Stevia rebaudiana</i>under Water Stress

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American Journal of Plant Sciences 2014年第22期5卷 3417-3422页

作者： shilpi Srivastava Malvika Srivastava Plant Physiology and Biochemistry Laboratory Department of Botany D.D.U. Gorakhpur University Gorakhpur India

Stevia rebaudiana, a herbaceous perennial shrub contains steviol glycosides, as an alternative source of sugar for diabetic patients. Water being an integral part plays a vital role in the maintenance of plant life. Availability of water is one of the limiting factors determining plant distribution and survival in natural ecosystem. The objective of this study was to investigate the ability of tolerance of Stevia plants to water stress. Potted plants of Stevia were subjected to different levels of water regimes (100 ml, 200 ml, 300 ml) per day, whereas control plants were watered daily with about 400 ml water. Plant height, leaf area, electrolyte leakage and antioxidant enzyme activity (peroxidase and catalase) were assayed during the experimental period. All these parameters were severely affected under water stress condition. Stress treatment caused an increase in electrolyte leakage compared to control. Plant height decreased under severe stress condition whereas a sharp increase in antioxidant enzyme activity was observed in stressed plants as compared to untreated control plants. Our experiment emphasizes the importance of proper watering schedule for the cultivation of Stevia as an agricultural crop to meet the challenges for sugar and energy crisis.

关键词： Stevia Water Stress Growth Antioxidant Enzymes

Independent and combined association of parity and short pregnancy with obesity and weight change among Indian women

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Health 2012年第5期4卷 271-276页

作者： shilpi Gupta Satwanti Kapoor Department of Anthropology University of Delhi Delhi India

Objective: To investigate association of parity and short pregnancy with obesity and weight change in Aggarwal Baniya women. Method: A cross-sectional analysis was carried out on a representative sample of 307 adult Aggarwal Baniya women aged 30 - 50 years (mean age: 38.7 ± 4.87) using multistage cluster sampling method. Weight, height, various skinfold thicknesses, waist and hip circumference were measured using standardized protocol. Various indices of obesity (BMI, WHR, WHtR, GMT) were calculated subsequently. Comparison groups were defined by the number of births (parity), short pregnancies and total pregnancies. Mean change in weight and other obesity markers were examined for each group separately. Correlation analysis was applied to see the association of childbearing on obesity. Linear regression was applied as an effective measure. Results: There was a gain in weight (3.16 kg) and increase in other obesity markers (BMI: 1.29 kg/m2;WC: 2.38 cm;HC: 3.83 cm) with each increase in each parity. Significant and positive correlation (p 2). Conclusion: Among other risk factors, high parity number may be associated with obesity in women. Therefore, interventions to prevent obesity should be targeted at women prior to initiation of childbearing. However, the impact of reproductive wastage in the form of short pregnancies on women’s obesity needs further exploration.

关键词： Women Parity Short Pregnancy Weight Gain Central Obesity