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华蟾素调控AKT介导的上皮间质转化抑制肝细胞癌肺转移裸鼠模型的作用机制

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临床肝胆病杂志 2024年第9期40卷 1840-1847页

作者：杨悦续嗣钰王珏杜施霖张春蕾宋海燕上海中医药大学附属龙华医院脾胃病研究所上海200032 上海中医药大学附属龙华医院急诊科上海200032 复旦大学附属中山医院急诊科上海200032

目的研究华蟾素通过调控肝细胞癌(HCC)上皮间质转化(EMT)抑制HCC转移的作用和机制。方法将36只6周龄雄性BALB/c裸鼠尾静脉注射MHCC97H细胞建立肝癌肺转移瘤模型,随机分为华蟾素高、低剂量组和对照组。建模当日起分别腹腔注射华蟾素120μ... 详细信息

目的研究华蟾素通过调控肝细胞癌(HCC)上皮间质转化(EMT)抑制HCC转移的作用和机制。方法将36只6周龄雄性BALB/c裸鼠尾静脉注射MHCC97H细胞建立肝癌肺转移瘤模型,随机分为华蟾素高、低剂量组和对照组。建模当日起分别腹腔注射华蟾素120μL/kg、60μL/kg或生理盐水,每周2次。8周后取肺组织行HE染色检测肝癌肺转移率。MHCC97H细胞用华蟾素高、低剂量(2.5μL/m L、5μL/m L)干预,通过划痕实验、RT-PCR以及Western Blot检测细胞迁移能力和EMT相关分子的表达。用CoCl2孵育模拟低氧环境诱导MHCC97H细胞,同时加入高、低剂量华蟾素干预,通过划痕实验和Western Blot检测华蟾素对低氧诱导的细胞迁移能力和EMT的影响。使用转录组学分析华蟾素对MHCC97H细胞的效应机制。用Western Blot检验华蟾素干预对MHCC97H细胞的蛋白激酶B(AKT)、磷酸化AKT(P-AKT)表达水平的影响。计量资料多组间比较采用单因素方差分析,进一步两两比较采用LSD-t检验,两组间比较采用成组t检验。结果华蟾素干预组裸鼠较对照组肝癌肺转移率下降。与对照组相比,华蟾素干预使MHCC97H细胞划痕愈合率减小、上皮型分子表达上调(t=2.860,P<0.05),并使EMT转录因子和基质型分子下调(t值分别为3.545、2.022、2.852、2.341,P值均<0.05)。低氧诱导上调MHCC97H细胞划痕愈合率和基质型分子、EMT转录因子表达水平(P值均<0.05),华蟾素干预逆转EMT变化并抑制划痕愈合(P值均<0.05)。肝癌细胞转录组学分析显示,华蟾素组与对照组存在显著的基因差异,华蟾素主要影响了肿瘤、代谢、免疫和信号传导相关基因表达,其中AKT信号转导通路中的差异基因数量最多。进一步检测发现华蟾素干预可下调HCC细胞AKT、P-AKT和P-AKT/AKT的水平(t值分别为2.434、3.401、2.258,P值均<0.05)。结论华蟾素可抑制肝癌转移,尤其对于低氧环境诱导的肝癌转移具有显著抑制作用,调控AKT信号转导通路介导的HCC细胞EMT可能是其部分作用机制。

关键词：癌,肝细胞肿瘤转移华蟾素上皮-间质转化

冻融条件下基于核磁共振技术的膨胀土孔隙及抗剪强度研究

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冰川冻土 2024年第3期46卷 919-929页

作者：汪恩良刘承前刘兴超李宇昂邹亦云杜世林东北农业大学水利与土木工程学院黑龙江哈尔滨150030

为了探究季节冻土区膨胀土渠道边坡浅层滑动机理,与多向冻结条件对比,对单向冻结方式的土样开展核磁共振(NMR)试验、磁共振成像(MRI)试验、室内直剪试验。研究了单向开放补水冻结条件下膨胀土含水率和孔隙率变化与力学特性之间的联系。... 详细信息

为了探究季节冻土区膨胀土渠道边坡浅层滑动机理,与多向冻结条件对比,对单向冻结方式的土样开展核磁共振(NMR)试验、磁共振成像(MRI)试验、室内直剪试验。研究了单向开放补水冻结条件下膨胀土含水率和孔隙率变化与力学特性之间的联系。结果表明:通过磁共振成像试验可以得到水分迁移后更准确的含水率分布曲线。多向冻结条件下,冻融作用使土体内大孔隙数量减少,中孔数量增多,且孔径普遍减小。而单向开放补水冻结条件下,由于冰透镜体特殊的生长方式,导致土体中大、中孔隙数量均有所增加,且集中在冰透镜体发育位置。单向冻结土体原位冻结区的抗剪强度与多向冻结土体抗剪强度大致相等,分凝冻结区土体抗剪强度低于原位冻结区,对内摩擦角影响较大。并结合黑龙江北部引嫩干渠滑坡的调研情况,得到春融期微薄层状构造冰透镜体发育导致土体抗剪强度降低是季节冻土区膨胀土渠道边坡失稳的主要原因。

关键词：膨胀土冻融作用核磁共振抗剪强度

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建筑碳排放软件对比分析及计算研究

建筑施工 2024年第1期46卷 48-51页

作者：孙建伟蒋世龙马义奎姜张张杜石林中建三局第一建设工程有限责任公司江苏南京210019 南京工业大学土木工程学院江苏南京211816

为更好地控制建筑碳排放量,建筑碳排放的测算与管理至关重要。基于此,对PKPM-CES建筑碳排放计算软件V3、东禾建筑碳排放软件、绿建斯维尔建筑碳排放CEEB 2023等3款建筑施工领域常用的碳排放软件进行了对比研究,分析其适用范围和计算过程... 详细信息

为更好地控制建筑碳排放量,建筑碳排放的测算与管理至关重要。基于此,对PKPM-CES建筑碳排放计算软件V3、东禾建筑碳排放软件、绿建斯维尔建筑碳排放CEEB 2023等3款建筑施工领域常用的碳排放软件进行了对比研究,分析其适用范围和计算过程,通过实际案例对其计算结果进行对比分析并总结各自的优缺点,以期为技术人员选择软件提供参考,并为新软件的开发提供建议。

关键词：碳排放计算软件对比功能分析计算分析

CFD-PBM coupled modeling of the liquid-liquid dispersion characteristics and structure optimization for Kenics static mixer

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Chinese Journal of Chemical Engineering 2024年第6期70卷 173-188页

作者： Junhai Deng shilin Lan Juchang Wu Shenghua du Weidong Liu Luchang Han Yefeng Zhou National&Local United Engineering Research Centre for Chemical Process Simulation and Intensification Chemical Process Simulation and Optimization Engineering Research Center of Ministry of EducationXiangtan UniversityXiangtan 411105China Hunan Haili Chemical Industry Company Limited Changsha 410000China

Kenics static mixers(KSM)are extensively used in industrial mixing-reaction processes by virtue of high mixing efficiency,low power homogenization and easy continuous *** liquid droplet size and its distribution and thus revealing the dispersion characteristics are of great significance for structural optimization and process intensification in the *** this work,a computational fluid dynamics-population balance model(CFD-PBM)coupled method is employed to systematically investigate the effects of operating conditions and structural parameters of KSM on droplet size and its distribution,to further reveal the liquid-liquid dispersion *** indicate that higher Reynolds numbers or higher dispersed phase volume fractions increase energy dissipation,reducing Sauter mean diameter(SMD)of dispersed phase droplets and with a shift in droplet size distribution(DSD)towards smaller *** aspect ratios,greater blade twist and assembly angles amplify shear rate,leading to smaller droplet size and a narrower DSD in the smaller *** degree of impact exerted by the aspect ratio is notably ***,mixing elements with different spin enhance shear and stretching *** to the same spin,SMD becomes 3.7-5.8 times smaller in the smaller size range with a significantly narrower *** into account the pressure drop and efficiency in a comprehensive manner,optimized structural parameters for the mixing element encompass an aspect ratio of 1-1.5,a blade twist angle of 180°,an assembly angle of 90°,and interlaced assembly of adjacent elements with different *** work provides vital theoretical underpinning and future reference for enhancing KSM performance.

关键词： CFD Population balance Liquid-liquid dispersion Kenics static mixer

Composition regulates dissolved organic matter adsorption onto iron (oxy)hydroxides and its competition with phosphate: Implications for organic carbon and phosphorus immobilization in lakes

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Journal of Environmental Sciences 2024年第10期144卷 159-171页

作者： ShuaiLong Wen JingJing Liu YueHan Lu JiaRu Dai XiuLin Huang shilin An Erik Jeppesen ZhengWen Liu YingXun du State Key Laboratory of Lake Science and Environment Nanjing Institute of Geography and LimnologyChinese Academy of SciencesNanjing 210008China University of Chinese Academy of Sciences Beijing 100049China Molecular Eco-Geochemistry(MEG)Laboratory Department of Geological SciencesThe University of Alabama2017th AveTuscaloosaAL 35485USA School of Environmental Science and Engineering Nanjing Tech UniversityNanjing 210008China School of Environmental and Chemical Engineering Chongqing Three Gorges UniversityChongqing 404020China Department of Ecoscience Aarhus UniversitySilkeborg 8600Denmark Sino-Danish Centre for Education and Research University of Chinese Academy of SciencesBeijing 101408China Limnology Laboratory Department of Biological Sciences and Centre for Ecosystem Research and ImplementationMiddle East Technical UniversityAnkara 06800Turkey

Dissolved organicmatter(DOM)is a heterogeneous pool of compounds and exhibits diverse adsorption characteristics with or without phosphorous(P)*** impacts of these factors on the burial and mobilization of organic carbon and P in aquatic ecosystems remain *** this study,an algae-derived DOM(ADOM)and a commercially available humic acid(HA)with distinct compositions were assessed for their adsorption behaviors onto iron(oxy)hydroxides(FeOx),both in the absence and presence of *** contained less aromatics but more protein-like and highly unsaturated structures with oxygen compounds(HUSO)than *** adsorption capacity of FeOx was significantly greater for ADOM than for ***-like and HUSO compounds in ADOM and humic-like compounds and macromolecular aromatics in HA were preferentially adsorbed by ***,ADOM demonstrated a stronger inhibitory effect on phosphate adsorption than *** observation suggests that the substantial release of autochthonous ADOM by algae could elevate internal P loading and pose challenges for the restoration of restore eutrophic *** presence of phosphate suppressed the adsorption of protein-like compounds in ADOM onto FeOx,resulting in an increase in the relative abundance of protein-like compounds and a decrease in the relative abundance of humic-like compounds in post-adsorption *** contrast,phosphate exhibited no discernible impact on the compositional fractionation of ***,our results show the source-composition characters of DOM influence the immobilization of both DOM and P in aquatic ecosystems through adsorption *** preferential adsorption of proteinaceous compounds within ADOM and aromatics within HA highlights the potential for the attachment with FeOx to diminish the original source-specific signatures of DOM,thereby contributing to the shared DOM characteristics observed across diverse aquatic environments.

关键词： Algal dissolved organic matter Humic acid Adsorption Phosphate Fractionation FT-ICR MS

转型期国家干预之边界考量——基于经济法的认知视角

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甘肃社会科学 2006年第4期 15-18,9页

作者：杜仕林南方医科大学人文社会科学学院西南政法大学经济贸易法学院重庆400031

国家干预边界判定旨在将社会资源配置权在国家与市场间合理分配。鉴于经济法语境中国家干预特质、转型期特点与干预现状,转型期国家干预之边界判定应坚持市场优先、社会公共利益及动与静相结合三个基本原则,转型期国家干预之边界判定标... 详细信息

国家干预边界判定旨在将社会资源配置权在国家与市场间合理分配。鉴于经济法语境中国家干预特质、转型期特点与干预现状,转型期国家干预之边界判定应坚持市场优先、社会公共利益及动与静相结合三个基本原则,转型期国家干预之边界判定标准可从干预范围、干预对象与干预程度三个层次依次设定。

关键词：经济法国家干预边界判定原则与标准

HIV核酸定量检测在“艾滋病一站式诊疗服务”模式中的应用和效果

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中国艾滋病性病 2024年第6期30卷 591-595页

作者：杨涛杨孝敬刘亦菲姚仁敏周彦君杨金星杜仕林黄家艳周顺林元康蔡毅媛王应刚贵州医科大学公共卫生与健康学院环境污染与疾病监控教育部重点实验室贵阳561113 安顺市疾病预防控制中心贵州安顺561000 安顺市普定县人民医院贵州安顺562100

目的探讨HIV核酸定量检测在“艾滋病一站式诊疗服务”模式中的应用效果和可行性,为HIV/AIDS患者早期诊断、早期治疗和科学管理提供参考依据。方法在普定县人民医院开展“艾滋病一站式诊疗服务”模式探索,对HIV初筛有反应血液进行院内... 详细信息

目的探讨HIV核酸定量检测在“艾滋病一站式诊疗服务”模式中的应用效果和可行性,为HIV/AIDS患者早期诊断、早期治疗和科学管理提供参考依据。方法在普定县人民医院开展“艾滋病一站式诊疗服务”模式探索,对HIV初筛有反应血液进行院内核酸定量试验,并将HIV初筛有反应血液送至贵州省疾病预防控制中心进行抗体确证试验,将院内检测结果与复核结果进行比较,以验证核酸定量检测的可行性;同时对“艾滋病一站式诊疗服务”开展前后抗体初筛有反应到抗体确证或核酸定量试验结果的时间间隔、抗体初筛有反应到接受cART时间间隔、30天内接受cART比例进行对比分析。结果对新检测发现的HIV抗体初筛有反应就诊者血液进行核酸定量检测,结果>5 000拷贝/mL占比为94.97%(189/199),≤5 000拷贝/mL占比为4.02%(8/199),≤5 000拷贝/mL后期经随访均为阳性,未检出占比为1.01%(2/199);将HIV初筛有反应血液送至贵州省疾病预防控制中心进行复核,结果阳性197份,阴性2份;院内检测结果与复核结果的一致性较好(Kappa=1,P<0.001),总符合率为100.00%(199/199);核酸定量检测的灵敏度较好,为95.94%(189/197),特异度为100.00%(2/2);“艾滋病一站式诊疗服务”开展前后新报告病例抗体初筛有反应到抗体确证或核酸定量试验结果中位时间由8天缩短至1天、抗体初筛有反应到接受cART中位时间由16天缩短至1天,差异有统计学意义(P <0.001);开展前后新报告病例30天内接受cART比例由68.00%提升至99.49%(196/197),差异有统计学意义(P<0.001)。结论通过HIV核酸定量检测在“艾滋病一站式诊疗服务”模式中的应用,HIV核酸定量检测用于HIV感染诊断的可行性得到了有效验证,可以显著缩短HIV/AIDS患者从检测到治疗的时间,及时接受抗病毒治疗。

关键词：艾滋病病毒核酸定量检测艾滋病一站式诊疗服务应用效果

Tetrabromobisphenol A exerts thyroid disrupting effects but has little overt impact on postnatal brain development and neurobehaviors in mice

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Journal of Environmental Sciences 2024年第8期142卷 1-10页

作者： shilin Song Yuanyuan Li Lin Lv Mengqi Dong Zhanfen Qin State Key Laboratory of Environmental Chemistry and Ecotoxicology Research Center for Eco-Environmental SciencesChinese Academy of SciencesBeijing 100085China College of Resources and Environment University of Chinese Academy of SciencesBeijing 100049China School of Environment Tsinghua UniversityBeijing 100084China

Tetrabromobisphenol A(TBBPA)is a widely used brominated flame *** is evidence showing that TBBPA can exert thyroid disrupting effects in mammals,but different results were also reported,along with inconsistent reports regarding its ***,we investigated thyroid disrupting effects and neurotoxicity of TBBPA(5,50,500μg/(kg·day))to male mice following maternal and direct exposure through drinking water,with the antithyroid drug propylthiouracil(PTU)as the positive *** postnatal day(PND)15,we expectedly observed severe thyroid compensatory hyperplasia and cerebellar developmental retardation in PTU-treated *** highest dose of TBBPA also caused thyroid histological alteration but had no effects on cerebellar development in terms of Purkinje cell morphology and the thickness of the internal granular layer and the molecular layer of the *** puberty and adulthood,the thyroid morphological alterations became more pronounced in the TBBPA-treated animals,accompanied by decreased serum thyroid hormone ***,the 50 and 500μg/(kg·day)TBBPA groups showed a significant decrease in the serum level of serotonin,a neurotransmitter associated with anxiety ***,the highest dose group displayed anxiety-like behaviors in the elevated plus-maze test on PND 35,but this neurobehavioral alteration disappeared on PND ***,no changes in neurobehavioral parameters tested were found in TBBPAtreated animals at puberty and ***,all observations show that TBBPA can exert thyroid disrupting effects but has little overt impact on brain development and neurobehaviors in mice,suggesting that thyroid disruption does not necessarily cause overtly adverse neurodevelopmental outcomes.

关键词： Tetrabromobisphenol A Thyroid disruption Brain development Neurobehavior Cerebellum

Chemical Activation of S/Li2S in Li-S Batteries by a Bidirectional Organic Redox Mediator

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CHEMICAL RESEARCH IN CHINESE UNIVERSITIES 2024年第5期40卷 927-934页

作者： Li, Chengqiu Zhou, Chaoyong Mei, shilin Yao, Changjiang Beijing Inst Technol Sch Mechatron Engn State Key Lab Explos Sci & Safety Protect Beijing 100081 Peoples R China Xian Safty Energy Technol Co Ltd Xian 710299 Peoples R China

The energy density and lifespan of prototype Li-S batteries under high sulfur loading and lean electrolyte have been mainly restricted by the incomplete interconversion between insulating S8 and Li2S. The introduction of an electrocatalyst has been preserved as an effective way to breakthrough the bottleneck of the interconversion rate. Herein, we demonstrate a novel bidirectional redox mediator, insoluble dithiobisphthalimide (DTPI), as the electrocatalyst for both S8 reduction and Li2S oxidation. due to the dual-functional role of both electron/Li+ donor and acceptor, DTPI can efficiently accelerate the redox reactions during charge/discharge and significantly alleviate the incomplete conversion of sulfur species. Consequently, the Li-S batteries with DTPI deliver superior specific capacity and cycling stability in comparison with those without DTPI. Especially, the redox mediator is scalable for synthesis and the DTPI-based 5 Ah pouch cell delivers a specific discharge capacity of around 870 mAhg(-1) at 0.1 C (1 C=1675 mA/g) without capacity fading over 80 cycles. The bidirectional catalysis mechanism has been studied through theoretical calculation and ex-situ characterization of the cathode materials. This work approves the effectiveness of bidirectional organic redox mediator in the construction of practical Li-S batteries.

关键词： Lithium-sulfur battery (LSB) Dithiobisphthalimide (DTPI) Organic redox mediator Interconversion LITHIUM FRAMEWORK