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A combination of leaf rust resistance gene Lr34 and lesion mimic gene lm significantly enhances adult plant resistance to Puccinia triticina in wheat

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Chinese Science Bulletin 2012年第17期57卷 2113-2119页

作者： LI Tao BAI guiHua gu shiliang Jiangsu Provincial Key Laboratory of Crop Genetics and Physiology Key Laboratory of Plant Functional Genomics of Ministry of EducationYangzhou UniversityYangzhou 225009China Department of Plant Pathology Kansas State UniversityManhattanKS 66506USA USDA-ARS Hard Winter Wheat Genetics Research Unit ManhattanKS 66506USA

Leaf rust caused by Puccinia triticina is an economically-important disease in wheat worldwide.A combination of different types of resistance genes may significantly enhance rust resistance under rust-favorable *** investigate the interactions between the rust resistance gene Lr34 and the lesion mimic gene lm on 1BL in Ning 7840,a segregating F8-10 population of 180 recombinant inbred lines was developed from Ning 7840/Chokwang and evaluated for both lesion mimic expression and leaf rust response at the adult plant stage in a greenhouse.A major quantitative trait locus(QTL),derived from Sumai 3,was co-localized with Lr34 on chromosome 7D and explained 41.5% of phenotypic variations for rust severity and 22.1% for leaf tip necrosis(LTN).The presence of Lr34 was confirmed by Lr34-specific markers cssfr1 and cssfr2 in Ning 7840 and Sumai *** Lr34,lm conditioned a spontaneous lesion mimic phenotype and had a significant effect on reducing uredinial size,and a smaller effect on *** effects were observed between lm and Lr34 for severity and LTN,and an epistatic effect was observed for infection *** marker analysis also identified several other QTL with minor effects on severity,infection type,or LTN.

关键词：抗叶锈病基因小麦叶锈菌抗条锈病成株期病变模仿单标记分析数量性状位点

Molecular evolution and functional divergence of HAK potassium transporter gene family in rice(Oryza sativa L.)

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Journal of Genetics and Genomics 2009年第3期36卷 161-172页

作者： Zefeng Yang Qingsong Gao Changsen Sun Wenjuan Li shiliang gu Chenwu Xu jiangsu Provincial Key laboratory of Crop Genetics and Physiology Key Laboratory of Plant Functional Genomics of Ministry of Education Yangzhou University Yangzhou 225009 China School of Life Sciences Taizhou University Linhai 317000 China

The high-affinity K＋（HAK） transporter gene family is the largest family in plant that functions as potassium transporter and is important for various aspects of plant life. In the present study, we identified 27 members of this family in rice genome. The phylogenetic tree divided the land plant HAK transporter proteins into 6 distinct groups. Although the main characteristic of this family was established before the origin of seed plants, they also showed some differences between the members of non-seed and seed plants. The HAK genes in rice were found to have expanded in lineage-specific manner after the split of monocots and dicots, and both segmental duplication events and tandem duplication events contributed to the expansion of this family. Functional divergence analysis for this family provided statistical evidence for shifted evolutionary rate after gene duplication. Further analysis indicated that both point mutant with positive selection and gene conversion events contributed to the evolution of this family in rice.

关键词： HAK potassium transporter gene family rice functional divergence gene conversion positive selection

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生物数学学报 1997年第1期12卷 34-43页

作者： gu shiliang(Departement of Agronomy,Agricultural College,Yangzhou University,Yangzhou 225009) 扬州大学农学院江苏扬州 225009

A new similarity between two clustering solutions was proposed in this paper. Paired matching groups are formed and their similarity is defined as:Sj The similarity between two clustering solutions is by taking the average of all k matching groups similarity with weight coefficient:The dendrogram similarity is defined as:The comparison and analyses indicate that the new similarity is better than existing measures such as FM's Bk and others.

关键词： Cluster analysis dendrogram similarity

The Contraction-Expansion Algorithm and Its Use in Fitting Nonlinear Equation

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生物数学学报 1998年第4期13卷 426-434页

作者： gu shiliang Hui Dafeng Bian Aihua(Quantitative Genetics Lab,Agricultural college,Yangzhou University,Yangzhou,225009) 扬州大学农学院江苏扬州

A new optimization method,contraction-expansion algorithm,is devel-oped and applied to fit nonlinear *** points with equal distance are allocated in the given initial intervals,then the algorithm searches for the points of better objective function one round after another,with contracting and expanding intervals *** step length(l),a critical factor to optimal solution,is determined and adjusted by feedback information of the searching *** experiments with number of simulat-ed and real data sets show that the new algorithm is much easier to the optimization than other *** algorithm does not need to provide derivatives of the *** algorithm can also be used in other ptimization problems.

关键词： Optimization algorithm nonlinear equation fitting contraction-ex-pansion algorithm

Fusarium head blight resistance loci in a stratified population of wheat landraces and varieties

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Fusarium head blight resistance loci in a stratified populat...

Comparison and confirmation of quantitative trait loci conferring partial resistance to rice sheath blight on chromosome 9

中国植物病理学会第十二届青年学术研讨会

作者： Tao Li Dadong Zhang Xiali Zhou guihua Bai Lei Li shiliang gu Jiangsu Provincial Key Laboratory of Crop Genetics and Physiology/Co-Innovation Center for Modern Production Technology of Grain Crops Department of Agronomy Kansas State University USDA-ARS Hard Winter Wheat Genetics Research Unit

To determine if Chinese and Japanese wheat landraces and varieties have unique sources of Fusarium head blight（FHB） resistance,an association mapping panel of 195 wheat accessions including both commercial varieties and landraces was genotyped with 364 genome-wide simple sequence repeat（SSR） and sequence-tagged site（STS） markers,and evaluated for type Ⅱ FHB resistance in three greenhouse experiments using single floret *** structure analysis stratified this population into five groups with Chinese landraces in four ***-two of 51 Chinese landraces and 24 of 27 Japanese accessions were placed in one *** analysis using a mixed model identified 11 markers having significant associations with FHB resistance in at least two *** of these markers coincided with known quantitative trait loci（QTL） for FHB resistance with one potentially novel QTL associated with Xgdm138-5DS and ***19-3AS was significant in all three experiments,and the frequency of favorable alleles was more than53%.Chinese landraces and Japanese accessions had more favorable alleles at the majority of reproducible marker *** QTL combinations were identified according to the number of favorable *** FHB severities increased with decreasing numbers of favorable alleles at reproducible *** resistance loci characterized here will further diversify the wheat FHB resistance gene pool,and provide breeders with additional sources of resistance for improvement of FHB resistance in wheat.

关键词： allelic variation association analysis Fusarium graminearum Triticum aestivum wheat scab

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Comparison and confirmation of quantitative trait loci confe...

江苏省遗传学会2013年学术研讨会

作者： Shimin Zuo Yajun Zhu Yaojun Yin Hui Wang Yafang Zhang Zongxiang Chen shiliang gu Xuebiao Pan Key Lab for Crop Genetics and Physiology of Jiangsu Province/Key lab of Plant Functional Genomics Ministry of Education Yangzhou University

Sheath blight（SB）, caused by Rhizoctonia solani Kühn, is one of the worst rice（O. sativa） diseases worldwide. Resistance to the SB disease in rice is a complex trait controlled by quantitative trait loci（QTLs）. Through map integration, we found several previously identified SB resistance QTLs（SBR QTLs） reported in inconsistent regions on the long arm of chromosome 9. Five of them were detected on rice cultivars Jasmine 85（J85）, Minghui 63（MH63）, and Lemont（LMNT）, and were designated qSB-9 J85-1, q SB-9 J85-2, qSB-9 MH63-1, qSB-9 MH63-2 and qSB-9 LMNT, respectively, in the present study. To further verify and physically map the five potential SBR-QTLs, we introduced these SBR-QTLs into a common susceptible variety LMNT and developed a few chromosomal segment substitution lines through marker-assisted selection（MAS）. After artificial inoculation with the SB fungus, we were able to validate qSB-9 J85-2 but not for the rest four SBR QTLs;while on MH63, an SBR QTL, designated q SB-9 MH6-3, was confirmed in the region defined by markers Y83 and Y91.8 that included q SB-9 J85-2, covering approximately 1235 kb. Both qSB-9 J85-2 and qSB-9 MH63-3 appeared to be dominant resistance gene（s） and contributed to similar levels to SB resistance, reducing SB disease severity by approximately 1.0 in a ‘0-9’ SB disease rating system. After comparing with another confirmed SBR QTL（qSB-9 TQ） from rice cultivar Teqing（TQ）, we conclude that qSB-9 J85-2, qSB-9 MH63-3 and qSB-9 TQ are probably controlled by the same allelic resistance gene（s）. These results will accelerate the utilization of this major SBR-QTL and its map-based cloning.

关键词： rice(Oryza sativa) sheath blight quantitative trait locus location resistance effect

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动态聚类的一种新方法——最小组内平方和法

江苏农学院学报 1989年第4期10卷 1-8页

作者：顾世梁莫惠栋

本文提出一种以组内平方和之和最小为标准的新的动态聚类方法,简称为最小组内平方和法(MinSS_w法).Monte-Carlo试验表明,该法能有效地调整初始分组中的个体.使之达到合理的分类,并有着良好的稳健性;而目前常用的重心法(K-means法)的动... 详细信息

本文提出一种以组内平方和之和最小为标准的新的动态聚类方法,简称为最小组内平方和法(MinSS_w法).Monte-Carlo试验表明,该法能有效地调整初始分组中的个体.使之达到合理的分类,并有着良好的稳健性;而目前常用的重心法(K-means法)的动态聚类结果,却是相当不稳定的.上述结论也为一个含14个小麦品种的聚类实例所证实.

关键词：聚类分析动态聚类统计法

温室条件下小麦白粉病抗性的综合评价和高抗品种的筛选

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麦类作物学报 2016年第4期36卷 435-442页

作者：李韬李嫒嫒李磊顾世梁扬州大学江苏省作物遗传生理重点实验室/粮食作物现代产业技术协同创新中心/教育部植物功能基因组学重点实验室/小麦研究中心江苏扬州225009

为进一步培育高抗白粉病小麦品种提供材料,在温室白粉病高选择压和自然侵染条件下,先后在抽穗期和灌浆期,对80份小麦材料(地方品种、国内育成品种/系、国外引进品种及部分中国春非整倍体材料)的白粉病抗性情况进行调查记载,然后利用马... 详细信息

为进一步培育高抗白粉病小麦品种提供材料,在温室白粉病高选择压和自然侵染条件下,先后在抽穗期和灌浆期,对80份小麦材料(地方品种、国内育成品种/系、国外引进品种及部分中国春非整倍体材料)的白粉病抗性情况进行调查记载,然后利用马氏距离及其聚类法对不同材料的抗性进行多生育时期和多指标的综合评价。结果表明,国外引进品种Capo全生育期免疫白粉病,中国春衍生的大多非整倍体材料和国外引进品种Coop Capoildo表现高抗。依据各材料间的马氏距离将80份材料分为6类,Capo所在的第2类总体抗性最好,该类包含17份材料,抽穗期和灌浆期平均侵染型小于1.0,平均严重度小于10.0%。中国春及其衍生的非整倍体材料与Capo抗性较为相似,表明中国春携带的广谱抗性基因Lr34仍然具有优异的白粉病抗性。

关键词：小麦白粉病侵染型严重度马氏距离综合评价

小麦中基于转录组测序SNP的dCAPS标记的高通量开发及验证

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扬州大学学报（农业与生命科学版） 2018年第4期39卷 86-90页

作者：李磊贡豪顾世梁李韬扬州大学江苏省作物遗传生理重点实验室/教育部植物功能基因组学重点实验室江苏扬州225009

结合六倍体小麦品系P7001与P216杂交F5代群体中1对相对性状池的二代转录组测序的单核苷酸多态性(SNP)分析结果,应用最近开发的CAPS/dCAPS Designer工具进行批量的CAPS/dCAPS(衍生/限制性内切酶多态性)标记开发,最终筛选20个CAPS/dCAPS... 详细信息

结合六倍体小麦品系P7001与P216杂交F5代群体中1对相对性状池的二代转录组测序的单核苷酸多态性(SNP)分析结果,应用最近开发的CAPS/dCAPS Designer工具进行批量的CAPS/dCAPS(衍生/限制性内切酶多态性)标记开发,最终筛选20个CAPS/dCAPS标记进行亲本间的多态性验证。结果表明,将二代转录组测序的SNP分析结果和高通量的CAPS/dCAPS标记设计软件相结合能高效地进行多态性分子标记的开发。

关键词：小麦单核苷酸多态性 CAPS/dCAPS标记高通量开发