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Potential of essential oils for poultry and pigs

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Animal Nutrition 2018年第2期4卷 179-186页

作者： Hengxiao Zhai Hong Liu shikui wang Jinlong Wu Anna-Maria Kluenter DSM(China)Animal Nutrition Research Center DSM Nutritional Products Ltd.

The increasing pressure of abolishing and/or decreasing the use of antibiotics as antimicrobial growth promoters for livestock calls for alternative solutions to sustain the efficiency of current livestock production.Among the alternatives, essential oils have a great potential and are generally considered natural, less toxic, and free from residues.Essential oils have been proven in numerous in vitro studies to exert antimicrobial effects on various pathogens.The current review touched on the basics of essential oils, and the in vivo effects of essential oils on growth, intestinal microflora, anti-oxidation, immune functionality, meat qualities as well as the possible modes of action in poultry and pigs, and the future research areas were proposed.

关键词： Antibiotics Chickens Essential oils Pathogens Pigs

Growth performance of nursery and grower-finisher pigs fed diets supplemented with benzoic acid

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Animal Nutrition 2017年第3期3卷 232-235页

作者： Hengxiao Zhai Wen Ren shikui wang Jinlong Wu Patrick Guggenbuhl Anna-Maria Kluenter DSM (China) Animal Nutrition Research Center Co.Ltd. DSM Nutritional Products France Centre de Recherche en Nutrition Animale DSM Nutritional Products Ltd.

Two experiments were conducted to investigate the efficacy of benzoic acid on the growth performance of nursery and grower-finisher pigs. A randomized complete block design was used in both experiments with the initial body weight as the blocking factor. There were 3 treatments corresponding to 3 dietary levels of benzoic acid: 0, 0.3%, and 0.5%. In experiment 1, a total of 144 PIC L1050 barrows(initial body weight 7.1 ± 0.6 kg) were used with each treatment replicated 8 times. In experiment 2, a total of 288 PIC L1050 barrows(initial body weight 36.1 ± 3.6 kg) were used with each treatment replicated 16 times.There were 6 barrows in each replicate pen for both experiments. Experiments 1 and 2 lasted 28 and 70 days, respectively. In experiment 1, average daily gain(ADG) of all growth phases increased linearly(P < 0.05) with increasing supplementation of benzoic acid, which led to a linear improvement in average body weight on d 28(P < 0.05). There was also an improvement in feed conversion ratio(FCR) of d 0 to 14(linear effect: P < 0.05) and in average daily feed intake(ADFI) of d 14 to 28 and d 0 to 28(linear effect:P < 0.01). In experiment 2, ADG during d 0 to 35 and d 35 to 70 and average body weight on d 35 improved linearly(P < 0.05) with increasing supplementation of benzoic acid. Average daily gain of d 0 to70 and average body weight on d 70 increased significantly in a both linear and quadratic manner. There was a linear improvement in FCR in all growth phases(P < 0.05). In conclusion, the dietary inclusion of benzoic acid at the supplementation levels of 0.3% and 0.5% significantly improved the growth performance of nursery and grower-finisher pigs in the current study; the nursery pigs responded to the dietary supplementation of benzoic acid up to 0.5% linearly while the grower-finisher pigs achieved the optimal ADG at the calculated supplementation level of 0.36%.

关键词： Benzoic acid Nursery pig Grower-finisher pig Average daily gain Average daily feed intake Feed conversion ratio

The Experimental Exploration and Discovery of DNA Communication between the Plants

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Journal of Modern Physics 2022年第11期13卷 1499-1517页

作者： Xinzhou Yuan Jiafeng Yuan Zhongxian Deng shikui wang Zhen Yang Qiao Bi Shenzhen Xinzhou Biological Information Technology Co. Ltd. Shenzhen China

For the first time, through the invention of Compensating Bio-information Energy (CBE) technology and bioinformatics breeding machine, we have completed a number of experiments by using plant signals to transfer plant genetic traits in the same family or across families, and discovered the transfer phenomenon of life genetic information. The test results show that plants can change from random variation to controllable and directional variation, thus opening up plant asexual, no molecular transfer, fast and low-cost breeding. The new approach provides new evidence for the connection of information energy waves between plant DNA, which deserves the attention and in-depth study of the scientific community.

关键词： Biological Signal Biological Microwave Radiation DNA Communication Bio-Information Breeding Machine

黄芩苷通过ROS依赖性调节RhoA/ROCK通路保护氯化钴诱导心肌细胞损伤的实验研究

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海南医学院学报 2024年第7期30卷 481-488页

作者：沈艳玲刘承红王世魁徐尧张云波顾申红海南医学院海南省创伤与灾难救援研究重点实验室海南海口571199 海南医学院第一附属医院全科医学科海南海口570102

目的:本研究旨在探讨黄芩苷(Baicalin)对氯化钴(CoCl_(2))诱导H9c2心肌细胞缺氧损伤的作用机制。方法:采用CoCl_(2)建立心肌细胞缺氧损伤模型,并分别加入不同浓度的黄芩苷培养。将正常氧培养设置为对照组,CoCl_(2)培养H9c2心肌细胞设置... 详细信息

目的:本研究旨在探讨黄芩苷(Baicalin)对氯化钴(CoCl_(2))诱导H9c2心肌细胞缺氧损伤的作用机制。方法:采用CoCl_(2)建立心肌细胞缺氧损伤模型,并分别加入不同浓度的黄芩苷培养。将正常氧培养设置为对照组,CoCl_(2)培养H9c2心肌细胞设置为CoCl_(2)组;Baicalin、Y27632(Rho激酶抑制剂)预处理的H9c2缺氧心肌细胞设置为CoCl_(2)+Baicalin组、CoCl_(2)+Y27632组、CoCl_(2)+Baicalin+Y27632组。通过细胞毒性检测试剂盒(CCK8)检测细胞活性;荧光探针测定细胞中活性氧(ROS)的表达;WST-8检测心肌细胞超氧化歧化酶(SOD)活力;TBA法测定丙二醇(MDA)浓度;同时采用WesternBlot方法分析RhoA、ROCK1、ROCK2、TNF-α、IL-1β蛋白表达情况。结果:处理H9c2细胞24 h后,1 000μmol/L的CoCl_(2)和75μmol/L黄芩苷对治疗心肌细胞缺氧损伤具有较好的细胞活力。CoCl_(2)组细胞活性明显低于对照组,加入黄芩苷后可显著提高细胞活性(P<0.05);与对照组相比,CoCl_(2)组心肌细胞可上调ROS、MDA表达,下调SOD活力,升高RhoA、ROCK1、ROCK2、TNF-α、IL-1β蛋白表达水平,加入Baicalin后可逆转上诉蛋白的表达水平;与CoCl_(2)组相比,CoCl_(2)+Baicalin可抑制ROS、MDA的表达,上调SOD含量,下调RhoA、ROCK1、ROCK2、TNF-α、IL-1β蛋白表达水平,而加入Y27632(Rho激酶抑制剂)后则可显著增强Baicalin带来的保护作用。结论:Baicalin可减轻CoCl_(2)诱导H9c2心肌细胞缺氧损伤的炎症、氧化应激反应,其机制与抑制ROS依赖性RhoA/ROCK通路有关。

关键词：黄芩苷 RhoA/Rho相关激酶(ROCK)信号通路缺氧损伤模型

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5%香芹酚水剂对韭菜迟眼蕈蚊的田间防治

北京农学院学报 2024年第2期39卷 21-24页

作者：安树杰祝保英陈艳王晶王新陈怀勐王士奎胡雪芳北京市昌平区小汤山现代农业科技示范园管理中心北京102211 农业农村部规划设计研究院北京100125 北京农学院生物与资源环境学院北京102206 华植(河北)生物科技有限公司石家庄051432

【目的】评价5%香芹酚水剂对韭菜迟眼蕈蚊(Bradysia odoripahaga)田间防效,以及对韭菜生长安全性影响。【方法】以0.3%苦参碱水剂作为对照药剂,通过灌根方式考察5%香芹酚水剂对韭菜迟眼蕈蚊防治效果,并通过增产率和品质感官评价评估其... 详细信息

【目的】评价5%香芹酚水剂对韭菜迟眼蕈蚊(Bradysia odoripahaga)田间防效,以及对韭菜生长安全性影响。【方法】以0.3%苦参碱水剂作为对照药剂,通过灌根方式考察5%香芹酚水剂对韭菜迟眼蕈蚊防治效果,并通过增产率和品质感官评价评估其对韭菜生产安全性。【结果】田间试验表明,在韭菜迟眼蕈蚊发生初期施药1次,药后3~14 d,5%香芹酚水剂450.00 g(a.i.)/hm^(2)、600.00 g(a.i.)/hm^(2)处理对韭菜保株效果维持在49.10%~85.60%,药后21 d韭蛆虫口减退率和防治效果最高达76.26%和84.32%,对韭菜增产率达14.59%。【结论】5%香芹酚水剂对韭菜迟眼蕈蚊具有较好防治效果,对韭菜生长安全,可作为防治韭菜迟眼蕈蚊的绿色杀虫剂推广应用。

关键词：韭菜韭菜迟眼蕈蚊香芹酚防效田间试验

逆行腓肠神经营养血管皮瓣修复足踝部软组织缺损临床观察

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中华灾害救援医学 2024年第3期11卷 271-273页

作者：袁民韩国章官丕财王石奎高志远青岛市黄岛区区立医院手足外科山东青岛266400

目的探讨对足踝部软组织缺损患者采用逆行腓肠神经营养血管皮瓣修复方案的临床效果。方法纳入青岛市黄岛区区立医院骨科的80例足踝部软组织缺损患者作为研究对象,随机分为逆行腓肠神经营养血管皮瓣修复(研究组)、股前外侧皮瓣修复(对照... 详细信息

目的探讨对足踝部软组织缺损患者采用逆行腓肠神经营养血管皮瓣修复方案的临床效果。方法纳入青岛市黄岛区区立医院骨科的80例足踝部软组织缺损患者作为研究对象,随机分为逆行腓肠神经营养血管皮瓣修复(研究组)、股前外侧皮瓣修复(对照组)两个组,两组各40例。对比分析术后患者炎性因子指标,随访足踝部软组织缺损患者对术后外观满意度、皮瓣成活率及修复部位皮肤弹性。结果两组术前炎性因子评分无统计学意义(P>0.05),治疗后研究组低于对照组(P<0.05)有统计学意义;研究组治疗后非常满意(42.50%)、总满意率(97.50%)均明显高于对照组(12.50%,77.50%)(P<0.05);研究组治疗后皮瓣成活率39例(97.50%)高于对照组28例(70.00%),修复部位皮肤弹性改善且效果好于对照组(P<0.05)。结论将腓肠神经营养血管皮瓣修复方式用于修复足踝部软组织缺损患者,能够改善炎性因子指标,提升患者对术后外观的满意度,提高皮瓣成活率,有助于修复部位皮肤弹性,安全性更高。

关键词：外科皮瓣皮肤软组织损伤

The changes of community components and their horizontal patterns caused by highway construction in the Longitudinal Range-Gorge Region

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Chinese Science Bulletin 2007年第A2期52卷 213-224页

作者： LIU Jie CUI BaoShan DONG shikui wang Juan ZHAO ShuQing School of Environment Beijing Normal University Beijing 100875 China

拿 Dabao (从达利城市到 Baoshan 城市)并且 Sixiao (从 Simao 城市到 Xiaomengyang 镇)在例子，社区部件的变化和他们的水平模式由高速公路建设引起了的纵的范围峡区域的高速公路与一些数学方法在这篇文章被讨论，例如对比分析，差别... 详细信息

拿 Dabao (从达利城市到 Baoshan 城市)并且 Sixiao (从 Simao 城市到 Xiaomengyang 镇)在例子，社区部件的变化和他们的水平模式由高速公路建设引起了的纵的范围峡区域的高速公路与一些数学方法在这篇文章被讨论，例如对比分析，差别分析等等。结果显示：1 ) 在高速公路建设，占优势的种类和亚 predominant 的骚乱下面，路边上的乔木和灌木植物的种类数量将减少到某程度，草本的植物的将增加很多，并且某入侵的种类将出现并且散布。2 ) 在路边上由高速公路建设，占优势的种类的分布和亚 predominant 种类影响了将从制服或随机的模式变化聚集模式，并且他们的总数度和扩散性将显然增加。3 ) 道路附近的 Ageratina adenophora 和 Chromolaena odorata 的平均范围度和高度远比那高离开道路，但是 2 种类的频率没在 200 m 以内在路边上有明显的变化。4 ) 道路节的位置和地形也是影响社区部件的重要因素，社区部件上的影响紧张由 Dabao 高速公路引起了的计算结果表演比由 Sixiao 高速公路，和在 2 不同地形会之间的影响紧张的比较结果引起了的高：山腹 > 山顶。

关键词：纵向岭谷区公路建筑物生态系统

Elemental Compositions of Clinopyroxenes from Southern Okinawa Trough Basalt:Implications for Magmatism and Structural Environment in an Initial Back-Arc Basin

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Journal of Ocean University of China 2024年第3期23卷 643-653页

作者： GUO Kun ZHAI shikui FAN Di ZHANG Xia ZHANG Yu ZHU Zhimin Laoshan Laboratory Qingdao 266237China Key Laboratory of Submarine Geosciences and Prospecting Techniques Ministry of EducationCollege of Marine GeosciencesOcean University of ChinaQingdao 266100China Key Laboratory of Submarine Geosciences&Second Institute of Oceanography Ministry of Natural ResourcesHangzhou 310012China

The major and trace elemental compositions of clinopyroxene from basalt were used to characterize the nature of the primitive magma and structural environment beneath the southern Okinawa Trough(SOT),which is an initial back-arc basin at a continental margin.The clinopyroxenes in the basalt were augite with variable Mg^(#)contents(73.37-78.22).The regular variations in major oxide contents(i.e.,CaO,FeO,TiO_(2),and Cr)with decreasing Mg#implied that the clinopyroxenes evolved from being enriched in Mg,Ca,and Cr to being enriched in Fe and Ti.The clinopyroxenes had relatively low rare earth element concentrations(7.51×10^(-6) to 12.68×10^(-6))and negative Eu anomalies(δEu=0.67-0.95).The Kd_(cpx) values of clinopyroxenes(0.2-0.26),which were used to examine whether the clinopyroxene was equilibrated with its host basalt,demonstrate that these clinopyroxene phenocrysts were not captured crystals but were instead produced by crystallization differentiation of the magma.The calculated clinopyroxene crystallization temperatures showed a narrow range of 990-1061℃,and their crystallization pressures ranged from 2.0 to 3.2 kbar.The geochemistry features of these clinopyroxenes indicated that the parent magma belonged to the subalkaline tholeiitic magma series and suggested that the magma experienced crystallization differentiation of olivine,plagioclase,and clinopyroxene,where the crystallization of plagioclase occurred earlier than that of clinopyroxene.Combined with geophysical data,this research on primitive magma and its crystallization differentiation from clinopyroxene indicates that the SOT is in the stage of‘seafloor spreading’and that basaltic rocks produced from tholeiitic magma represent the generation of oceanic crust.

关键词： trace elements clinopyroxenes southern Okinawa Trough seafloor spreading back-arc basin

醋酸预处理-熔融制样-X射线荧光光谱法测定重晶石中主要组分

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冶金分析 2023年第3期43卷 27-33页

作者：李大勇王士魁王亮张周位贵州省地质矿产中心实验室贵州省地矿局一一三地质大队

准确测定重晶石的主要组分对重晶石资源后续的开发与利用具有重要意义。由于X射线荧光光谱法(XRF)测定BaSO4、SrSO4的结果是通过测定Ba、Sr分析谱线后换算得到的，故样品中的BaCO3、SrCO3会干扰BaSO4和SrSO4的测定。实验先采用醋酸预处... 详细信息

准确测定重晶石的主要组分对重晶石资源后续的开发与利用具有重要意义。由于X射线荧光光谱法(XRF)测定BaSO4、SrSO4的结果是通过测定Ba、Sr分析谱线后换算得到的，故样品中的BaCO3、SrCO3会干扰BaSO4和SrSO4的测定。实验先采用醋酸预处理分离样品中的碳酸盐类成分，再采用XRF测定，成功避免了BaCO3、SrCO3对BaSO4和SrSO4测定的干扰。同时，将经过醋酸预处理后的滤渣灼烧后称重，计算样品损失量，通过补加与样品损失相等质量的混合熔剂的方法来补正因醋酸预处理及灼烧带来的样品质量损失，采用四硼酸锂-偏硼酸锂混合熔剂熔融制样，实现了XRF对重晶石中BaSO4、Al2O3、SiO2、Fe、SrSO4共5种组分的同时测定。选用各组分含量呈一定阶梯的重晶石国家标准物质及贵州省级标准物质绘制校准曲线，校准曲线的决定系数不小于0.999 8,BaSO4方法检出限为0.128%,其余组分的检出限范围为0.004%～0.029%。将实验方法应用于重晶石样品分析，结果表明，各组分测定值大于5%时，相对标准偏差RSD≤0.2%;各组分测定值不大于5%时，RSD≤2.0%。相对误差(RE)均小于地质矿产实验室测试质量管理规范DZ/T 0130—2006要求的允许限。

关键词：醋酸预处理熔融制样 X射线荧光光谱法(XRF) 重晶石硫酸钡硫酸锶