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不同肝炎患者及献血员中SENV-D/H感染流行病学调查

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临床血液学杂志（输血与检验） 2007年第5期20卷 201-203页

作者：陈蕤穆士杰张献清安群星吴原茹西安第四军医大学西京医院输血科西安710032

目的:了解SEN病毒D/H亚型在不同肝炎患者和献血员中的感染情况。方法:采用巢式聚合酶链反应对收集的健康献血者和各型肝炎患者进行SENV-D亚型和H亚型DNA检测。结果:无偿献血者中SENV-D的感染率为25.9%,SENV-H为24.4%;急性甲肝患者中SEN... 详细信息

目的:了解SEN病毒D/H亚型在不同肝炎患者和献血员中的感染情况。方法:采用巢式聚合酶链反应对收集的健康献血者和各型肝炎患者进行SENV-D亚型和H亚型DNA检测。结果:无偿献血者中SENV-D的感染率为25.9%,SENV-H为24.4%;急性甲肝患者中SENV-D的感染率为14.5%,SENV-H为32.7%;慢性乙肝患者中SENV-D的感染率为46%,SENV-H为45.2%;在丙肝患者中SENV-D和SENV-H的感染率均为65%;在非甲-非戊型肝炎患者中SENV-D的感染率为51.2%,SENV-H为43.9%。结论:SENV可能与HCV和HBV具有一样的传播方式。

关键词： SENV-D亚型 SENV-H亚型流行病学巢式聚合酶链反应

天花粉蛋白突变体TCS_(KR173-174CG)生物活性及免疫原性研究

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陕西医学杂志 2008年第3期37卷 259-261,265页

作者：安群星穆士杰张献清陈蕤雷迎峰余瑞刘宁徐志凯第四军医大学西京医院输血科西安710032 第四军医大学微生物学教研室

目的:对中草药成分天花粉蛋白(TCS)进行定点突变,使其免疫原性有所降低而生物活性不受或少受影响。方法:选择TCS分子上可能的抗原决定簇位点(KR173-174)进行定点突变,并将所构建的突变体(TCSKR173-174CG)基因在大肠杆菌中表达及纯化。... 详细信息

目的:对中草药成分天花粉蛋白(TCS)进行定点突变,使其免疫原性有所降低而生物活性不受或少受影响。方法:选择TCS分子上可能的抗原决定簇位点(KR173-174)进行定点突变,并将所构建的突变体(TCSKR173-174CG)基因在大肠杆菌中表达及纯化。随后与野生型TCS(wTCS)比较,分析其DNA酶活性、致核糖体失活活性、免疫原性和急性毒性。结果:初步检测发现,所构建的突变型TCS活性与wTCS活性几乎相当,而免疫原性有所降低。结论:通过该方法改造TCS是可行的,有可能为抗HIV治疗提供一种高效、低毒、廉价的药物。

关键词：天花粉素/免疫学 HIV/免疫学基因转变 @生物活性免疫组织化学

Long-time precipitation reduction and nitrogen deposition increase alter soil nitrogen dynamic by influencing soil bacterial communities and functional groups

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Pedosphere 2020年第3期30卷 363-377页

作者： Guoyong YAN Yajuan XING shijie HAN Junhui ZHANG Qinggui WANG Changcheng mu School of Forestry Northeast Forestry UniversityHarbin 150040China College of Agricultural Resource and Environment Heilongjiang UniversityHarbin 150080China College of Life Sciences Henan UniversityKaifeng 475004China institute of Applied Ecology Chinese Academy of SciencesShenyang 110016China

The effects of precipitation reduction and nitrogen deposition increase on soil bacterial communities and functions impact soil nitrogen cycling. Seasonal changes could modify the effects of precipitation reduction and nitrogen deposition increase on bacterial communities and functions by changing soil environments and properties. Understanding soil microbial communities and the seasonal response of functions to precipitation reduction and nitrogen deposition increase may be important for the accurate prediction of changes in the soil nitrogen dynamics. Thus, a long-term field simulation experiment of nitrogen deposition increase and throughfall exclusion was established to investigate soil bacterial communities’ response to nitrogen deposition increase and/or precipitation reduction, with no nitrogen deposition increase and no precipation reduction as a control, in a temperate forest. We examined soil bacterial communities(Illumina sequencing) under different treatments during the winter, freezing-thawing cycle periods(FTCs), and growing season. The bacterial functional groups were predicted by the FAPROTAX database. The results showed that nitrogen deposition increase, precipitation reduction, the combined effect of nitrogen deposition increase and precipitation reduction, and seasonal changes significantly altered the soil bacterial community ***, by combining the result of a previous study in which nitrogen deposition increase increased the nitrous oxide flux in the same experimental system, the loss of soil nitrogen was increased by the decrease in denitrification and increase of nitrification bacteria under nitrogen deposition increase,while ammonification bacteria significantly increased and N-fixing bacteria significantly decreased with precipitation reduction compared to the control. In relation to seasonal changes, the aromatic-degrading, cellulolytic, and ureolytic bacteria were lowest during FTCs, which indicated that FTCs might inh

关键词： copiotrophic bacteria denitrification multidimensional effect nitrification oligotrophic bacteria seasonal changes soil acidification water stress

反义hTERT基因对K562细胞凋亡及周期的影响

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现代肿瘤医学 2014年第1期22卷 33-37页

作者：陈晓鹏袁青安宁穆士杰张献清安群星第四军医大学西京医院输血科陕西西安710032 第四军医大学西京医院急诊科陕西西安710032

目的:探讨反义hTERT基因抑制K562细胞端粒酶活性对其细胞周期及增殖的影响。方法:用脂质体介导反义hTERT基因和空载体质粒转染K562细胞株,绘制生长曲线,MTT法检测细胞活性,血清饥饿法同步化细胞周期,流式细胞仪检测细胞周期及细胞凋亡... 详细信息

目的:探讨反义hTERT基因抑制K562细胞端粒酶活性对其细胞周期及增殖的影响。方法:用脂质体介导反义hTERT基因和空载体质粒转染K562细胞株,绘制生长曲线,MTT法检测细胞活性,血清饥饿法同步化细胞周期,流式细胞仪检测细胞周期及细胞凋亡率变化。结果:反义hTERT基因转染后,细胞生长明显受到抑制。细胞同步化后,流式细胞仪检测显示:KA组细胞出现早期凋亡峰,细胞阻滞于S期。但正常培养48h,各组细胞无明显凋亡,周期无显著差异。结论:反义hTERT基因可明显抑制K562的增殖,细胞同步化后具有促凋亡作用。

关键词：端粒酶反义hTERT K562细胞细胞凋亡细胞周期

三氧自体输血对改善脑卒中恢复期患者运动功能的疗效分析

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中国输血杂志 2023年第2期36卷 105-108页

作者：陈扬吴群强李颖王晓清穆士杰赵树铭空军军医大学第二附属医院输血科陕西西安710038 空军军医大学第二附属医院康复科陕西西安710038 贵黔国际总医院

目的探讨三氧自体输血在改善脑卒中恢复期患者意识状态和运动功能中的疗效。方法选取2016年8月~2021年9月在本院康复科接受治疗并确诊为脑卒中恢复期的患者共44例,采用盲法随机分为实验组(n=22,三氧自体输血治疗,采集患者肘正中静脉血20... 详细信息

目的探讨三氧自体输血在改善脑卒中恢复期患者意识状态和运动功能中的疗效。方法选取2016年8月~2021年9月在本院康复科接受治疗并确诊为脑卒中恢复期的患者共44例,采用盲法随机分为实验组(n=22,三氧自体输血治疗,采集患者肘正中静脉血200 mL,间隔2~3 d 1次,12次为1个疗程,同时进行一般康复训练(即运动疗法、作业疗法、针灸治疗、经颅磁刺激技术治疗)和对照组(一般康复训练),对2组患者治疗前后的意识状态(GCS评分)、日常生活能力(Barthel指数评分)得分情况统计与比较分析,并观察评估上田敏运动功能分级、Brunnstrom分期、肌张力分级。结果实验(治疗)结束后,实验组与对照组比较:1)GCS评分为14.82±0.39 vs 12.41±2.52、Barthel评分为61.14±12.24 vs 52.05±11.72(P<0.05);2)运动功能恢复率为95.45%(21/22)vs 63.64%(14/22)(P<0.05);3)肌张力总恢复率为90.91%(20/22)vs 63.64%(14/22)(P<0.05)。结论三氧自体输血对于脑卒中恢复期患者的意识状态、运动功能和肌张力有一定的改善作用,具备较高的安全性和有效性。

关键词：三氧治疗脑卒中恢复自体输血意识状态运动功能

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临床用血服务质量安全管理标准研究

中华医院管理杂志 2018年第12期34卷 1004-1008页

作者：胡丽华穆士杰钱宝华谢珏査占山陈凤花李归宁耿帜刘丽华华中科技大学同济医学院附属协和医院输血科武汉430022 空军军医大学唐都医院输血科海军军医大学长海医院输血科浙江大学医学院附属第一医院输血科解放军总医院医院管理研究所

本研究针对临床用血服务中输血管理、输血前、输血中、输血后的质量安全问题,制定相应的约束标准。应用文献分析、输血不良事件分析、制度标准解读等方法梳理国内外临床用血服务中的质量安全问题,按问题的普遍性、高发性、关键性、影响... 详细信息

本研究针对临床用血服务中输血管理、输血前、输血中、输血后的质量安全问题,制定相应的约束标准。应用文献分析、输血不良事件分析、制度标准解读等方法梳理国内外临床用血服务中的质量安全问题,按问题的普遍性、高发性、关键性、影响度4个方面筛选出问题要素内容。搭建了以临床用血服务流程为主线,以事项要素为节点的标准框架,形成了包括4个环节、20个关键要素的临床用血服务标准文本。

关键词：输血质量控制安全管理关键要素管理标准

Ab Initio Design of Ni-Rich Cathode Material with Assistance of Machine Learning for High Energy Lithium-Ion Batteries

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Energy & Environmental Materials 2024年第6期7卷 74-83页

作者： Xinyu Zhang Daobin mu shijie Lu Yuanxing Zhang Yuxiang Zhang Zhuolin Yang Zhikun Zhao Borong Wu Feng Wu School of Materials Science and Engineering Beijing Institute of TechnologyBeijing100081 China

With the widespread use of lithium-ion batteries in electric vehicles,energy storage,and mobile terminals,there is an urgent need to develop cathode materials with specific ***,existing material control synthesis routes based on repetitive experiments are often costly and inefficient,which is unsuitable for the broader application of novel *** development of machine learning and its combination with materials design offers a potential pathway for optimizing ***,we present a design synthesis paradigm for developing high energy Ni-rich cathodes with thermal/kinetic simulation and propose a coupled image-morphology machine learning *** paradigm can accurately predict the reaction conditions required for synthesizing cathode precursors with specific morphologies,helping to shorten the experimental duration and *** the model-guided design synthesis,cathode materials with different morphological characteristics can be obtained,and the best shows a high discharge capacity of 206 mAh g^(−1)at 0.1C and 83%capacity retention after 200 *** work provides guidance for designing cathode materials for lithium-ion batteries,which may point the way to a fast and cost-effective direction for controlling the morphology of all types of particles.

关键词： design digital image lithium-ion batteries machine learning NCM cathode

Definition of failure criterion for frozen soil under directional shear-stress path

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Research in Cold and Arid Regions 2019年第6期11卷 428-434页

作者： Dun Chen Wei Ma GuoYu Li ZhiWei Zhou YanHu mu shijie Chen State Key Laboratory of Frozen Soils Engineering Northwest Institute of Eco-Environment and ResourcesCASLanzhouGansu 730000China State Key Laboratory for GeoMechanics and Deep Underground Engineering China University of Mining and TechnologyXuzhouJiangsu 221116China Da Xing'anling Observation and Research Station of Frozen-Ground Engineering and Environment Northwest Institute of Eco-Environment and ResourcesChinese Academy of SciencesJiagedaqiHeilongjiang 165000China

A series of directional shear tests on remolded frozen soil was carried out at 10°C by using a hollow cylinder apparatus to study failure criterion under a directional shear-stress *** shear tests were conducted at five shear rates(10,20,30,40,and 50 kPa/min)and five intermediate principal stress coefficients(b=0,0.25,0.5,0.75,and 1),with the mean principal stress(p=4.5 MPa)kept *** results show that the torsional strength and the generalized strength both increase with the increase of the shear *** to the failure modes of frozen soil under different shear rates,the specimens present obvious plastic failure and shear band;and the torsional shear component dominates the failure modes of hollow cylindrical specimens.A shear rate of 30 kPa/min is chosen as the loading rate in the directional shear tests of frozen *** shape of the failure curve in theπplane is dependent on the directional anglesαof the major prin cipal *** is reasonable to use the strain-hardening curves to define the deviatoric stress value atγg=15%(generalized shear strain)as the failure criterion of frozen soil under a directional shear-stress path.

关键词： frozen soil hollow cylinder apparatus intermediate principal stress coefficient failure criterion directional shear-stress path

Stable cycling of LiNi_(0.9)Co_(0.05)Mn_(0.05)O_(2)/lithium metal batteries enabled by synergistic tuning the surface stability of cathode/anode

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Journal of Energy Chemistry 2023年第12期87卷 342-350,I0009页

作者： Chunli Li Xinyu Zhang Zhuolin Yang Haijian Lv Tinglu Song shijie Lu Yuxiang Zhang Tianwen Yang Fan Xu Feng Wu Daobin mu School of Materials Science&Engineering Beijing Key Laboratory of Environmental Science and EngineeringBeijing Institute of TechnologyBeijing 100081China College of Chemistry Beijing University of Chemical TechnologyBeijing 100029China Shenzhen BTR New Energy Technology Institute Co. LtdShenzhen 518118GuangdongChina

Ni-rich layered oxides(LiNi_(0.9)Co_(0.05)Mn_(0.05)O_(2))show great potential in long-range and low-cost lithiumion ***,due to the high surface sensitivity,their practical application is hindered by interfacial instability with electrolytes under high voltage for long cyclic ***,by combining both firstprinciple calculations and time-of-flight secondary ion mass spectrometry(TOF-SIMS),a novel surface fluorinated reconstruction(SFR)mechanism is proposed to improve the interfacial stability under high voltage,which could effectively regulate the surface fluoride species to desensitize the LiNi_(0.9)Co_(0.05)Mn_(0.05)O_(2)*** demonstrate here that by tuning the ratio of fluoride species,the LiNi_(0.9)Co_(0.05)Mn_(0.05)O_(2)/Li battery could achieve excellent long-term and high voltage performance(163.5 mA h g^(-1)at 0.5 C for 300 cycles under 4.4 V),while the controlled sample decayed to 125.4 mA h g^(-1)after 300 ***,the favorable cross-talk effect induced by SFR further facilitates the incorporation of suitable amounts of Ni ions into the construction of stable solid electrolyte interface(SEI)layer for anode ***,the ultra-long cycling stability under high voltage can be achieved by the robust cathode/electrolyte and Li/electrolyte interfaces,which results in excellent interfacial stability after long *** work provides new insights into the surface design of cathode materials and improves the stability of the electrode-electrode interface under high voltage.

关键词： Ni-rich layered oxides Surface fluorinated reconstruction Interfacial stability TOF-SIMS Cross-talk effect