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Molecular Mechanism of KDM5B Development in Hepatocellular Carcinoma

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Journal of Cancer Therapy 2024年第1期15卷 1-12页

作者： shijian fu Min Guo Dean’s Office The Fourth People’s Hospital of Haikou Haikou China

Objective: To investigate the mechanism of cell cyclin-dependent kinase (KDM5B), a key enzyme driving all cell cycle transitions, promoting HCC progression and metastasis. Methods: The expression of KDM5B in normal liver, HCC and its adjacent tissues was analyzed by RT-PCR and IHC. Lentivirus transfection method was used to construct stable cell lines with KDM5B overexpression and down-regulation, and the role of KDM5B in HCC migration and invasion was detected at cell level and animal level. Western blotting and Transwell experiments were performed to verify the effect of KDM5B and/or CCR2 inhibitors on HCC progression and metastasis by using liver orthotopic transplantation tumor model and immunofluorescence methods. Results: RT-PCR showed that the expression level of KDM5B in HCC was significantly higher than that in adjacent tissues, and the increase of KDM5B was relatively significant. Upregulation of KDM5B in nude mouse liver orthotopic transplantation tumor model can promote the incidence of lung metastasis and shorten the survival time of nude mice, whereas upregulation of KDM5B can reduce the incidence of lung metastasis and prolong the survival time of nude mice. Conclusion: This study clarified the expression of KDM5B in HCC and its function in promoting HCC migration, invasion and metastasis. The molecular mechanism of KDM5B promoting HCC metastasis was revealed, providing a potential therapeutic target for HCC.

关键词： Liver Cancer Cyclin-Dependent Kinase (KDM5B) Pathology Molecular Mechanism

Ecotoxicological effects of waterborne PFOS exposure on swimming performance and energy expenditure in juvenile goldfish (Carassius auratus)

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Journal of Environmental Sciences 2013年第8期25卷 1672-1679页

作者： Jigang Xia shijian fu Zhendong Cao Jianglan Peng Jing Peng Tingting Dai Lili Cheng Laboratory of Evolutionary Physiology and Behavior Chongqing Key Laboratory of Animal Biology Chongqing Normal University

The potential risks of perfluorooctane sulfonate （PFOS） are of increasing ecological concern. Swimming performance is linked to the fitness and health of fish. However, the impacts of PFOS on swimming performance remain largely unknown. We investigated the ecotoxicological effects of acute exposure to PFOS on the swimming performance and energy expenditure of juvenile goldfish （Carassius auratus）. The fish were exposed to a range of PFOS concentrations （0, 0.5, 2, 8 and 32 mg/L） for 48 hr. The spontaneous swimming activity, fast-start swimming performance, critical swimming speed （Ucrit） and active metabolic rate （AMR） of the goldfish were examined after exposure to PFOS. PFOS exposure resulted in remarkable effects on spontaneous activity. Motion distance was reduced, and the proportion of motionless time increased with increasing concentrations of PFOS. However, no significant alterations in the fast-start performance-related kinematic parameters, such as latency time, maximum linear velocity, maximum linear acceleration or escape distance during the first 120 msec after stimulus, were observed after PFOS exposure. Unexpectedly, although PFOS exposure had marked influences on the swimming oxygen consumption rates and AMR of goldfish, the U crit of the goldfish was not significantly affected by PFOS. This may result in a noteworthy increase in the energetic cost of transport. The overall results indicate that, in contrast to spontaneous activity, underlying swimming capabilities are maintained in goldfish after short-term exposure to PFOS, but energy expenditure during the process of swimming is dramatically aggravated.

关键词： perfluorooctane sulfonate （PFOS） spontaneous activity fast-start performance critical swimming speed energy expenditure goldfish

光照周期对两种鱼类社会性及社会奖赏情景下行为策略的影响

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生态学杂志 2024年第4期43卷 959-966页

作者：曾足仙龙家兴何娇阳付世建重庆师范大学进化生理与行为学实验室重庆市动物生物学重点实验室重庆401331

群体互动有助于成员的行为发展和生长发育,探究社会互动过程中社会奖赏的机制、表现和影响因素具有重要的意义。为了考察光照周期改变对鱼类社会性及社会奖赏情景下的决策行为的影响,本研究选取亲缘关系较远、群体互动特征不同且生境光... 详细信息

群体互动有助于成员的行为发展和生长发育,探究社会互动过程中社会奖赏的机制、表现和影响因素具有重要的意义。为了考察光照周期改变对鱼类社会性及社会奖赏情景下的决策行为的影响,本研究选取亲缘关系较远、群体互动特征不同且生境光照周期状况迥异的中华倒刺鲃(Spinibarbus sinensis)和雪鲷(Pseudotropheus zebra)作为实验对象,分析了短光照周期处理的两种鱼类在六臂迷宫探索时针对社会奖赏刺激鱼群的决策行为特征参数。结果表明:与中华倒刺鲃相比,雪鲷表现出更高的探索性和群体互动;雪鲷在群体决策时间上体现出集体智慧;短光照对雪鲷个性行为没有显著影响,但导致雪鲷社会互动时间和个体决策精度的下降;短光照导致中华倒刺鲃探索行为的上升和凝聚力的下降,但对决策的时间、精度以及群体互动时间均没有显著影响;光照周期对鱼类个性特征和群体互动影响存在种间差异,总体上对中华倒刺鲃的群体个性行为影响更为明显,对雪鲷的决策策略影响更为显著。这可能与二者社会互动和个性特征差异以及生境光周期特点相关。

关键词：短光照周期中华倒刺鲃雪鲷决策勇敢性凝聚力

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饥饿对中华倒刺鲃和慈鲷鱼群觅食行为的影响

重庆师范大学学报（自然科学版） 2024年第1期41卷 14-19页

作者：何娇阳龙家兴曾足仙付世建重庆师范大学进化生理与行为学实验室重庆市动物生物学重点实验室重庆401331

探究种类和饥饿对不同生境和集群特征鱼类的群体觅食行为的影响。选取群体互动特征不同、生境和食物资源状况迥异的鲤科(Cyprinidae)鱼类中华倒刺鲃(Spinibarbus sinensis)和慈鲷科(Cichlidae)鱼类斑马拟丽鱼(Pseudotropheus zebra)(后... 详细信息

探究种类和饥饿对不同生境和集群特征鱼类的群体觅食行为的影响。选取群体互动特征不同、生境和食物资源状况迥异的鲤科(Cyprinidae)鱼类中华倒刺鲃(Spinibarbus sinensis)和慈鲷科(Cichlidae)鱼类斑马拟丽鱼(Pseudotropheus zebra)(后简称慈鲷)幼鱼作为实验对象,采用六臂迷宫来设置觅食情景,分析比较了前期经历饥饿或维持日粮摄食(对照组)鱼群的探索强度、凝聚力指标(IC)、首次到达食物臂速度、食物臂时间占比等行为学参数。与中华倒刺鲃相比,慈鲷有更高的探索强度和更低的IC值。饥饿导致慈鲷探索强度下降、首次到达食物臂速度加快和食物臂时间占比上升;中华倒刺鲃经历饥饿后食物臂时间占比略有下降。慈鲷和中华倒刺鲃群体觅食行为存在种间差异,且仅有慈鲷在群体觅食行为上表现出对食物匮乏的适应性变化,这可能与2种鱼类生境、食物资源等条件不同有关。

关键词：中华倒刺鲃斑马拟丽鱼鲤科慈鲷科六臂迷宫饥饿

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抗痛风双靶标抑制剂研究进展

化学与生物工程 2024年第6期41卷 7-12页

作者：黄冬倩郑明束福李诗健刘根炎武汉工程大学化工与制药学院绿色化工过程教育部重点实验室新型反应器与绿色化学工艺湖北省重点实验室湖北武汉430205 鸣洋(武汉)化工工程有限公司湖北武汉430200

痛风是由尿酸单钠晶体沉积在关节和非关节结构处引起的常见代谢类疾病。治疗痛风的单靶标药物通常伴有严重不良反应,而双靶标药物具有更好的疗效和更低的毒性,因此开发能够降低体内血清尿酸水平的双靶标抑制剂是近年来抗痛风药物的研究... 详细信息

痛风是由尿酸单钠晶体沉积在关节和非关节结构处引起的常见代谢类疾病。治疗痛风的单靶标药物通常伴有严重不良反应,而双靶标药物具有更好的疗效和更低的毒性,因此开发能够降低体内血清尿酸水平的双靶标抑制剂是近年来抗痛风药物的研究热点。系统综述了具有降尿酸作用双靶标抑制剂及其衍生物的研究进展,为新型抗痛风双靶标药物的研发提供了参考。

关键词：尿酸痛风双靶标抑制剂

富含双键咪唑啉在饱和CO_(2)盐水中缓蚀行为研究

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石油与天然气化工 2024年第2期53卷 78-86页

作者：邹斌梁伟盖平原陈晓春程仕键付朝阳中国石化胜利油田分公司山东省稠油开采技术省级重点实验室中国石化新疆新春石油开发有限责任公司华中科技大学化学与化工学院

目的合成含有两个双键的亚油酸咪唑啉,与传统的含有单个双键油酸咪唑啉对比,研究其结构对其缓蚀性能的影响。方法在60℃下将Q235钢浸泡于饱和CO_(2)的3%(w)NaCl溶液中,通过失重、电化学测试和缓蚀效率测试,并通过表面分析方法观察腐蚀... 详细信息

目的合成含有两个双键的亚油酸咪唑啉,与传统的含有单个双键油酸咪唑啉对比,研究其结构对其缓蚀性能的影响。方法在60℃下将Q235钢浸泡于饱和CO_(2)的3%(w)NaCl溶液中,通过失重、电化学测试和缓蚀效率测试,并通过表面分析方法观察腐蚀后钢片形貌变化,最后通过理论计算对合成缓蚀剂进行性能研究。结果电化学与失重结果显示在加入质量浓度为200 mg/L的亚油酸咪唑啉后,缓蚀效率能达到90%,缓蚀性能优于常见的油酸咪唑啉。在结构上,由于亚油酸咪唑啉分子比油酸咪唑啉多了一个双键,增加了咪唑啉缓蚀剂的吸附位点,使其能更好地吸附于金属的表面。结论亚油酸咪唑啉对碳钢有很好的缓蚀性能;在同等实验条件下,缓蚀性能优于油酸咪唑啉;其吸附满足Langmuir吸附等温线;吸附过程中主要通过N—Fe键吸附在Fe表面。

关键词：缓蚀剂亚油酸咪唑啉电化学评价量子化学计算分子动力学模拟

Mesopore-encaged V-Mn oxides:Progressive insertion approach triggering reconstructed active sites to enhance catalytic oxidative desulfuration

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Chinese Journal of Chemical Engineering 2022年第5期35卷 182-193页

作者： fu Yang Ruyi Wang shijian Zhou Xuyu Wang Yan Kong Shuying Gao School of Environmental and Chemical Engineering Jiangsu University of Science and TechnologyZhenjiang 212003China State Key Laboratory of Materials-Oriented Chemical Engineering College of Chemical EngineeringNanjing Tech UniversityNanjing 210009China

The oxidative desulphurization(ODS)has become mainly popular by rapid catalytic oxidation of dibenzothiophene(DBT)relied on efficient heterogeneous catalyst,V-based catalytic active species were regarded as the potential option in the activity-preferred ODS systems.Herein,we reported the redispersion of vanadium oxide(VO_(χ))on the mesoporous silica modified with manganese oxide(Mn_(3)O_(4)) through one progressive insertion approach of metal oxides in the silica.Impressively,mesoporeencaged vanadium-manganese oxides in the silica(VMn-MS)as the admirable output of excellent ODS catalyst was demonstrated compared to other monometal-modified counterparts and one-pot implanted one.The characterization results revealed the post-implanted VO_(χ) species not only deposited around the pre-covered Mn_(3)O_(4) on the mesoporous surface but also inserted the surface layer of Mn_(3)O_(4) inducing the amorphous evolution of aggregated Mn_(3)O_(4) and the reconstruction of final active sites.This integrated approach made the reconstructed active species afford more exposed catalytic sites and the tailored surface redox cycles owing to the electronic communication of V-Mn.The catalytic results demonstrated the excellent catalytic desulphurization efficiency(~100%)during 60 min at 80℃,which made the sulphur content reduce to 6 mg·L^(-1),remarkably superior to other comparative samples.The outstanding catalytic performance of VMn-MS catalyst can be ascribed to the synergistic effect of V-Mn dual metals rendering two different reaction pathways,which includes free-radical reaction and ring-forming reaction,where Mn site acted as active center triggering reactive free radicals which could be further optimized by surrounded V sites around Mn sites to promote the ODS process.

关键词： Nanomaterials Moleclar sieves Oxidative desulphurization Radicals Synergetic effect Surface

基于血清代谢组学研究佛手柑内酯治疗肝纤维化的作用机制

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中国药房 2024年第13期35卷 1570-1575页

作者：吴辉星张振华龙昌锐郭桂芬王炎玉陈燕纯付钜雄乡世健周本杰鲁澄宇广东医科大学药学院广东东莞523808 中山大学附属第七医院药学部广东深圳518107 惠东县人民医院血液净化中心广东惠州513600 深圳市中药活性物质筛选与转化重点实验室广东深圳518107

目的基于血清代谢组学技术研究佛手柑内酯(BP)治疗肝纤维化的作用及机制。方法将40只小鼠分为正常对照组(0.5%羧甲基纤维素钠溶液)、模型组(0.5%羧甲基纤维素钠溶液)和BP低、高剂量组(50、100 mg/kg),每组10只。除正常对照组外其余3... 详细信息

目的基于血清代谢组学技术研究佛手柑内酯(BP)治疗肝纤维化的作用及机制。方法将40只小鼠分为正常对照组(0.5%羧甲基纤维素钠溶液)、模型组(0.5%羧甲基纤维素钠溶液)和BP低、高剂量组(50、100 mg/kg),每组10只。除正常对照组外其余3组小鼠均采用四氯化碳诱导肝纤维化模型。同时,各组小鼠灌胃相应药物/溶剂,每日1次,连续8周。末次给药后,检测小鼠血清中丙氨酸转氨酶(ALT)、天冬氨酸转氨酶(AST)水平,观察小鼠肝组织病理形态学变化,检测小鼠肝组织中α-平滑肌肌动蛋白(α-SMA)、Ⅰ型胶原蛋白(CollagenⅠ)表达,同时对小鼠血清进行代谢组学分析。结果与模型组比较,BP低、高剂量组小鼠血清中ALT、AST水平和肝组织中α-SMA、CollagenⅠ蛋白表达水平均显著降低(P<0.05),肝组织的纤维化程度显著改善。代谢组学结果显示,BP高剂量组和模型组共有175个血清差异代谢物,其中18个物质上调、157个物质下调,涉及的主要代谢途径有嘧啶代谢、丁酸盐代谢、脂肪酸合成、酪氨酸代谢、β-丙氨酸代谢、烟酸和烟酰胺代谢以及谷胱甘肽代谢等。结论 BP可能通过调节肝纤维化小鼠血清中嘧啶代谢、丁酸盐代谢和谷胱甘肽代谢等途径达到治疗肝纤维化的作用。

关键词：佛手柑内酯肝纤维化血清代谢组学差异代谢物代谢途径

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ANALYSIS OF THE CHAOTIC MOTION FOR A ROTOR SYSTEM WITH A TRANSVERSE CRACK

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Acta Mechanica Solida Sinica 2003年第1期16卷 74-80页

作者： fu Yiming Zheng Yufang Zhu shijian Department of Engineering Mechanics Hunan UniversityChangsha 410082China Institute of Noise and Vibration University of Naval EngineeringWuhan 430033China

The chaotic motion of an elastic shaft-disk rotor system including thegeometric nonlinearity of the shaft with a transverse crack is investigated. The critical conditionfor the system to enter chaotic state is given by the Melnikov method. Meanwhile, whether the chaosoccurs is determined by the Poincare map, phase portrait and time-displacement history diagram.

关键词： geometric nonlinearity cracked rotor chaos