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Metabolome profiling of stratified seeds provides insight into the regulation of dormancy in Davidia involucrata

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Plant Diversity 2022年第4期44卷 417-427页

作者： Shiming Deng Qiang Xiao Cigui Xu Jian Hong Zhijun Deng Dan Jiang shijia luo Key Laboratory of Biologic Resources Protection and Utilization of Hubei Province Hubei Minzu UniversityEnshi 445000Hubei ProvinceChina Beijing Advanced Innovation Center for Tree Breeding by Molecular Design National Engineering Laboratory for Tree BreedingCollege of Biological Sciences and BiotechnologyBeijing Forestry UniversityBeijing 100083China

Dove tree(Davidia involucrata),a tertiary vestige species,is well-adapted to cool *** in *** seed lasts for an extremely long period of time,typically between 3 and 4 years,and this characteristic makes the species an excellent model for studying the mechanisms of seed *** molecular mechanisms governing germination control in *** are still *** stratification have been reported to enhance germination in recalcitrant *** performed a widely targeted metabolome profiling to identify metabolites and associated pathways in *** seeds from six different moist sand stratification durations(0-30 months) using the ultra-high-performance liquid chromatography-Q Exactive Orbitrap-Mass *** was an increasing germination rate with prolonged stratification durations(12-30 months).Furthermore,we detected 10,008 metabolites in the stratified *** also detected 48 differentially accumulated metabolites(DAMs) between all stratification periods in the seeds,with 10 highly conserved *** of the differentially accumulated metabolites between unstratified and stratified seeds were enriched in purine metabolism,pyrimidine metabolism,flavone and flavonol biosynthesis,phenylpropanoid biosynthesis,and arginine biosynthesis *** phytohormones,abscisic acid,indole-3 acetic acid,and sinapic acid were differentially accumulated in the seeds and are predicted to regulate dormancy in *** have provided extensive metabolic information useful for future works on dove tree germination study.

关键词： Dove tree Metabolome Dormancy Abscisic acid Stratification

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珙桐组织培养研究

林业科技 2006年第4期31卷 4-6页

作者：罗世家华中农业大学园艺林学院

分别以MS、N6和B5为基本培养基,添加NAA与6-BA不同浓度组合于培养基中,以珙桐的叶片、茎、芽为外植体,诱导愈伤组织。结果表明:N6培养基的诱导愈伤效果略好于MS培养基和B5培养基,但效果不显著。同时使用NAA和6-BA时,以NAA2.0 mg/L加6-BA... 详细信息

分别以MS、N6和B5为基本培养基,添加NAA与6-BA不同浓度组合于培养基中,以珙桐的叶片、茎、芽为外植体,诱导愈伤组织。结果表明:N6培养基的诱导愈伤效果略好于MS培养基和B5培养基,但效果不显著。同时使用NAA和6-BA时,以NAA2.0 mg/L加6-BA1.0 mg/L时效果较好。叶片、茎、芽3种外植体以芽诱导愈伤组织效果最明显。

关键词：珙桐外植体愈饬组织组织培养

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论应用型大学经济管理类教材的策划

出版发行研究 2009年第12期 33-35页

作者：罗时嘉孙浩中国矿业大学出版社

应用型大学是我国高等教育体系的重要组成部分,其教材的开发理应受到出版社的重视。本文以应用型大学经济管理类教材为例,对现有应用型大学教材的问题进行分析,根据应用型大学经济管理专业的特点,提出相关的策划思路。

关键词：应用型大学经济管理类教材策划思路

LncRNA CCRR maintains Ca^(2+)homeostasis against myocardial infarction through the FTO-SERCA2a pathway

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Science China(Life Sciences) 2024年第8期67卷 1601-1619页

作者： Hua Yang Lina Xuan Shengjie Wang Huishan luo Xiaomeng Duan Jianjun Guo shijia Cui Jieru Xin Junwei Hao Xiufang Li Jun Chen Feihan Sun Xiaolin Hu Siyun Li Ying Zhang Lei Jiao Baofeng Yang Lihua Sun Department of Pharmacology Harbin Medical University(State Key Laboratory of Frigid Zone Cardiovascular Diseasethe State-Province Key Laboratories of Biomedicine-Pharmaceutics of ChinaKey Laboratory of Cardiovascular ResearchMinistry of EducationJoint International Research Laboratory of Cardiovascular Medicine ResearchMinistry of EducationChina)College of PharmacyHarbin Medical UniversityHarbin 150081China

Cardiac conduction regulatory RNA(CCRR)has been documented as an antiarrhythmic lncRNA in our earlier *** study aimed to evaluate the effects of CCRR on SERCA2a and the associated Ca^(2+)homeostasis in myocardial infarction(MI).Overexpression of CCRR via AAV9-mediated delivery not only partially reversed ischemia-induced contractile dysfunction but also alleviated abnormal Ca^(2+)homeostasis and reduced the heightened methylation level of SERCA2a following *** effects were also observed in CCRR overexpressing transgenic mice.A conserved sequence domain of CCRR mimicked the protective function observed with the full ***,silencing CCRR in healthy mice led to intracellular Ca^(2+)overloading of *** increased SERCA2a protein stability by upregulating FTO *** direct interaction between CCRR and FTO protein was characterized by RNA-binding protein immunoprecipitation(RIP)analysis and RNA pulldown *** of NFATc3 was identified as an upstream mechanism responsible for CCRR downregulation in *** study demonstrates that CCRR is a protective lncRNA that acts by maintaining the function of FTO,thereby reducing the m^(6)A RNA methylation level of SERCA2a,ultimately preserving calcium homeostasis for myocardial contractile function in ***,CCRR may be considered a promising therapeutic strategy with a beneficial role in cardiac pathology.

关键词： CCRR calcium homeostasis FTO myocardial infarction SERCA2a

Global multifaceted biodiversity patterns,centers,and conservation needs in angiosperms

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Science China(Life Sciences) 2024年第4期67卷 817-828页

作者： Ao luo Yaoqi Li Nawal Shrestha Xiaoting Xu Xiangyan Su Yichao Li Tong Lyu Kilara Waris Zhiyao Tang Xiaojuan Liu Luxiang Lin Yongsheng Chen Kuiling Zu Wenqi Song shijia Peng Niklaus E.Zimmermann Loïc Pellissier Zhiheng Wang Institute of Ecology and Key Laboratory for Earth Surface Processes of the Ministry of Education College of Urban and Environmental SciencesPeking UniversityBeijing 100871China Department of Health and Environmental Sciences Xi’an Jiaotong-Liverpool UniversitySuzhou 215123China State Key Laboratory of Grassland Agro-Ecosystems College of EcologyLanzhou UniversityLanzhou 730000China Key Laboratory of Bio-Resource and Eco-Environment of Ministry of Education College of Life SciencesSichuan UniversityChengdu 610065China Land Consolidation and Rehabilitation Center Ministry of Natural ResourcesBeijing 100035China State Key Laboratory of Vegetation and Environmental Change Institute of BotanyChinese Academy of SciencesBeijing 100093China CAS Key Laboratory of Tropical Forest Ecology Xishuangbanna Tropical Botanical GardenChinese Academy of SciencesKunming 650201China Swiss Federal Research Institute WSL Birmensdorf 8903Switzerland Department of Environmental Systems Science ETH ZürichZürich 8092Switzerland

The Convention on Biological Diversity seeks to conserve at least 30%of global land and water areas by 2030,which is a challenge but also an opportunity to better preserve biodiversity,including flowering plants(angiosperms).Herein,we compiled a large database on distributions of over 300,000 angiosperm species and the key functional traits of 67,024 *** this database,we constructed biodiversity-environment models to predict global patterns of taxonomic,phylogenetic,and functional diversity in terrestrial angiosperms and provide a comprehensive mapping of the three diversity *** further evaluated the current protection status of the biodiversity centers of these diversity *** results showed that geographical patterns of the three facets of plant diversity exhibited substantial spatial mismatches and nonoverlapping conservation *** centers of functional diversity,particularly of herbaceous species,were primarily distributed in temperate regions and under weaker protection compared with other biodiversity centers of taxonomic and phylogenetic *** global assessment of multifaceted biodiversity patterns and centers highlights the insufficiency and unbalanced conservation among the three diversity facets and the two growth forms(woody ***),thus providing directions for guiding the future conservation of global plant diversity.

关键词： angiosperms species richness phylogenetic diversity functional diversity plant traits woody species herbaceous species

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顾及地理语义的运动轨迹相似性度量模型

测绘通报 2023年第3期 67-73,149页

作者：周慧君罗世佳蒋和平张晶首都师范大学水资源安全北京实验室北京100048 首都师范大学三维信息获取与应用教育部重点实验室北京100048 首都师范大学城市环境过程虚拟仿真国家级实验教学中心北京100048 首都师范大学资源环境与旅游学院北京100048

移动对象的运动行为往往受到所处地理环境的影响,针对现有轨迹相似性分析无法考虑地理环境因素的现状,本文提出了一种顾及地理语义的多重运动特征轨迹相似性度量方法。该方法首先以扩展到时间层面的数据立方体为基础,对轨迹序列的地理... 详细信息

移动对象的运动行为往往受到所处地理环境的影响,针对现有轨迹相似性分析无法考虑地理环境因素的现状,本文提出了一种顾及地理语义的多重运动特征轨迹相似性度量方法。该方法首先以扩展到时间层面的数据立方体为基础,对轨迹序列的地理语义和运动特征进行符号化;然后利用多个特征值计算得到的加权编辑距离作为衡量轨迹相似性的标准;最后将该相似性度量方法与谱聚类结合,利用真实数据验证了该度量模型的有效性与优势。

关键词：地理环境地理语义运动轨迹运动特征时空立方体

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车前叶蓝蓟籽油提取方法和溶剂的优化

分子植物育种 2018年第23期16卷 7821-7826页

作者：江喻罗世家邓志军刘欢邓仕明湖北民族学院生物资源保护与利用湖北省重点实验室恩施445000 湖北民族学院林学园艺学院恩施445000

本研究应用了加压液体萃取(pressurized liquid extraction,PLE)、微波辅助萃取(microwave assisted extraction,MAE)和超声辅助萃取(ultrasound assistedextraction,UAE)三种现代提取技术对车前叶蓝蓟籽油进行提取,并对乙酸乙酯、乙醇、水及其混合液作为溶剂的萃取条件进行了优化,以实现作为正己烷的替代绿色萃取溶剂。本研究表明,乙醇是一种作为车前叶蓝蓟籽油提取的非常有优势的绿色提取溶剂。PLE提取产量最高(32.1%),但温度也相对较高,可能导致部分不稳定化合物热降解。UAE(29.8%)的主要特点是在温和条件下进行的,低温(60℃)允许使用UAE作为热不稳定化合物的提取。另一方面,无论是开放系统(22.6%)还是封闭系统(24.9%),MAE都显示出所有测试技术的最低产油量。然而,MAE的优势主要是节省时间,并且可以每隔几分钟可重复进行一次快速有效的萃取。

关键词：车前叶蓝蓟籽油加压液体萃取微波辅助萃取超声辅助萃取

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纳米流体及分散剂对重力热管性能影响试验

江苏大学学报（自然科学版） 2017年第5期38卷 544-548页

作者：王助良戴世佳王宏宇罗明黄彬马青江苏大学能源与动力工程学院江苏镇江212013

为了研究纳米流体及分散剂对重力热管性能的影响,对各热管的启动性能(启动时间和启动温度)以及传热性能(稳定运行时管壁温度分布和总热阻的大小)进行了测试.结果表明:纳米颗粒的添加及其物理性质对热管的启动时间有影响,添加纳米流体的... 详细信息

为了研究纳米流体及分散剂对重力热管性能的影响,对各热管的启动性能(启动时间和启动温度)以及传热性能(稳定运行时管壁温度分布和总热阻的大小)进行了测试.结果表明:纳米颗粒的添加及其物理性质对热管的启动时间有影响,添加纳米流体的热管,启动时间较传统去离子水热管明显缩短1.3~3.2 min;分散剂的添加对热管的传热性能也有影响,在高热流密度工况下,以十二烷基硫酸钠(SDS)为分散剂的热管性能明显优于以阿拉伯树胶(AG)为分散剂的纳米流体热管;添加分散剂的纳米流体热管热阻较去离子水热管降低26.4%~63.9%.

关键词：纳米流体重力热管分散剂启动性能传热性能

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湘赣浙紫色土的类型与分布特点

山地研究 1993年第4期11卷 235-240页

作者：唐时嘉陈学华罗有芳中国科学院水利部成都山地灾害与环境研究所成都610041

湘赣浙紫色土主要分布在沅江、湘江、赣江、信江、衢江流域几个NE或NNE向平行排列的紫色丘陵盆地内,出露上白垩统一下第三系红色碎屑岩,可见中丘中谷、低丘宽谷和缓丘带坝三类地形,分布不同风化程度的成土风化壳和紫色土类型。三省酸性... 详细信息

湘赣浙紫色土主要分布在沅江、湘江、赣江、信江、衢江流域几个NE或NNE向平行排列的紫色丘陵盆地内,出露上白垩统一下第三系红色碎屑岩,可见中丘中谷、低丘宽谷和缓丘带坝三类地形,分布不同风化程度的成土风化壳和紫色土类型。三省酸性紫色土和中性紫色土比例较大,达73％,比四川盆地多38—39％。

关键词：紫色土风化壳湘赣浙类型分布