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Tracking Cryptosporidium in urban wastewater treatment plants in a cold region:Occurrence,species and infectivity

Frontiers of Environmental Science & Engineering 2022年第9期16卷 1-14页

作者： Dan Xiao Zhaofeng Lyu shiheng Chen yang Huo Wei Fan Mingxin Huo Jilin Academy of Agricultural Sciences Changchun 130033China School of Chemical Engineering Changchun University of TechnologyChangchun 130012China School of Environment Northeast Normal UniversityChangchun 130117China

This study investigated the occurrence,species,infectivity and removal efficiency of Cryptosporidium *** typical wastewater treatment *** from different process units were collected seasonally and synchronously from four wastewater treatment plants(WWTPs)in Northeastern *** Cryptosporidium oocysts were identified in most samples from both influent(97.50%)and effluent(90.00%)wastewaters of the four WWTPs,at an average density of 26.34 and 4.15 oocysts/L,*** overall removal efficiency was 84.25%,and oocysts were mainly removed(62.01%)by the modified secondary sedimentation *** Cryptosporidium species were identified in the effluent ***,***,and *** were the three most prevalent *** viability assays indicated no reduction of excystation rate during the primary and secondary wastewater treatments(varied in the range of 63.08%–68.50%),but the excystation rate declined to 52.21%in the effluent after ***,the Cryptosporidium oocysts showed higher infection intensity in the cold season(winter and spring)than that in summer and *** influences of environmental temperature on virulence factors of Cryptosporidium were further *** was observed that more extracellular secretory proteins were bound on the oocyst surface and several virulence genes were expressed relatively strongly at low temperatures,both of which could facilitate oocyst adhesion,invasion,and host immune *** research is of considerable interest since it serves as an important step towards more accurate panoramic recognition of Cryptosporidium risk reduction in WWTPs,and especially highlights the potential health risk associated with Cryptosporidium in cold regions/seasons.

关键词： WWTPs Cryptosporidium Occurrence Species Infectivity Low temperature

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Argon Plasma-Induced Graft Polymerization of PEGMA on Chitosan Membrane Surface for Cell Adhesion Improvement

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Plasma Science and Technology 2013年第10期15卷 1041-1046页

作者： YIN shiheng REN Li WANG Yingjun Analytical and Testing Center South China University of Technology Cuangzhou 510640China College of Materials Science and Engineering South China University of TechnologyGuangzhou 510640 China

For its biocompatibility and biodegradability, chitosan has had considerable atten- tion for biomedical applications in recent years. In this paper, polymerization of poly （ethylene glycol） methyl ether methacrylate （PEGMA） was grafted onto chitosan membrane surface through argon plasma-induced graft polymerization. The surface properties after modification were characterized by contact angle measurement, X-ray photoelectron spectroscopy （XPS） and atomic force microscopy （AFM）, respectively. The results indicated that PEGMA can be grafted successfully onto chitosan membrane surface. The surface hydrophilicity and free energy were improved and the surface roughness increased after modification. The adhesion of a human corneal epithelial cell （HCEC） on chitosan membrane surface was enhanced due to improvement of surface free energy and roughness.

关键词： chitosan membrane PEGMA plasma surface energy cell adhesion

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Cloning and characterization of a bursicon-regulated gene Su（H） in the house fly Musca domestica

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Insect Science 2009年第3期16卷 207-217页

作者： Songjie Wang shiheng An David Stanley Qisheng Song Division of Plant Sciences University of Missouri Columbia MO 65211 USDAIAgricultural Research Service Biological Control of Insects Research Laboratory 1503 S. Providence Road Columbia MO 65203 USA

Bursicon is a neuropeptide that regulates cuticle sclerotization （hardening and tanning） in insect via a G-protein coupled receptor. However, the signal transduction pathway downstream of the G-protein coupled receptor is currently not well known. In our recent microarray analysis, we identified a panel of genes regulated by bursicon in Drosophila. One of the genes, Suppressor of Hairless, or Su（H）, has drawn our attention because its product acts down-stream of the bursicon receptor. In the present study, we cloned the Drosophila homolog, mdSu（H）, from the house fly Musca domestica using 3＇ and 5＇ rapid amplification of complementary DNA ends. Real-time polymerase chain reaction analysis revealed that the level ofmdSu（H） transcript is up-regulated by ~3-fold 1 h after recombinant bursicon injection, which correlates well with the cuticle sclerotization process observed in the recombinant bursicon-injected flies. We infer that Su（H） is an essential gene involved in the insect cuticle sclerotization process.

关键词： cuticle sclcrotization gene regulation Musca domestica Notch pathway,r-bursicon suppressor of hairless

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MgO(001) barrier based magnetic tunnel junctions and their device applications

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Science China(Physics,Mechanics & Astronomy) 2013年第1期56卷 29-60页

作者： HAN XiuFeng ALI Syed Shahbaz LIANG shiheng State Key Laboratory of Magnetism Beijing National Laboratory for Condensed Matter PhysicsInstitute of PhysicsChinese Academy of Sciences

Spintronics has received a great attention and significant interest within the past decades,and provided considerable and remarked applications in industry and electronic information *** spintronics,the MgO based magnetic tunnel junction(MTJ) is an important research advancement because of its physical properties and excellent performance,such as the high TMR ratio in MgO based *** present an overview of more than a decade development in MgO based *** review contains three main sections.(1) Research of several types of MgO based MTJs,including single-crystal MgO barrier based-MTJs,double barrier MTJs,MgO based MTJs with interlayer,novel electrode material MTJs based on MgO,novel barrier based MTJs,novel barrier MTJs based on MgO,and perpendicular MTJs.(2) Some typical physical effects in MgO based MTJs,which include six observed physical effects in MgO based MTJs,namely spin transfer torque(STT) effect,Coulomb blockade magnetoresistance(CBMR) effect,oscillatory magnetoresistance,quantum-well resonance tunneling effect,electric field assisted magnetization switching effect,and spincaloric effect.(3) In the last section,a brief introduction of some important device applications of MgO based MTJs,such as GMR & TMR read heads and magneto-sensitive sensors,both field and current switching MRAM,spin nano oscillators,and spin logic devices,have been provided.

关键词： magnetic tunnel junction (MTJ) tunneling magnetoresistance (TMR) MgO spin transfer torque (STT) Coulombblockade magnetoresistance (CBMR)

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Sequence and epitope analysis of surface proteins of avian influenza H5N1 viruses from Asian patients

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Chinese Science Bulletin 2006年第20期51卷 2472-2481页

作者： LI Guanglin TAO shiheng WANG Xiujie College of Life Science Northwest A&F University Yangting 712100 China State Key Laboratory of Plant Genomics Institute of Genetics and Developmental Biology Chinese Academy of Sciences Beijing 100101 China

Increasing cases of human infections with the high pathogenic avian influenza virus H5N1 have raised great concern on potential human flu pandemics caused by H5N1. The two viral surface glycoproteins, the hemagglutinin (HA) and the neuraminidase (NA) proteins, are major antigens o H5N1. Introducing new mutations on these two pro teins is the major strategy used by H5N1 to expand host range and to avoid the recognition of host im mune systems. We analyzed the two surface proteins of H5N1 from Asian human patients and identified many new mutation sites, including a few that were unique to certain lethal strains. We also analyzed the distribution of mutations on different epitopes of the two surface proteins. A receptor-binding site tha might involve in the determination of host specificity of H5N1 was also found. Results reported here pro vided information for better understanding of the evolution trend of H5N1 genome in human.

关键词：禽流感基因分析受体结合部位寄主专一性亚洲

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Plasma graft of poly(ethylene glycol) methyl ether methacrylate (PEGMA) on RGP lens surface for reducing protein adsorption

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Plasma Science and Technology 2017年第1期19卷 51-56页

作者： shiheng YIN Li REN Yingjun WANG Analytical ＆ Testing Center South China University of Technology Guangzhou 510640 People's Republic of China College of Materials Science and Engineering South China University of Technology Guangzhou 510640 People's Republic of China

Poly（ethylene glycol） methyl ether methacrylate（PEGMA） was grafted on fluorosilicone acrylate rigid gas permissible contact lens surface by means of argon plasma induced polymerization to improve surface hydrophilicity and reduce protein *** surface properties were characterized by contact angle measurement,x-ray photoelectron spectroscopy（XPS） and atomic force microscopy *** surface protein adsorption was evaluated by lysozyme solution immersion and XPS *** results indicated that a thin layer of PEGMA was successfully *** surface hydrophilicity was bettered and surface free energy *** lysozyme adsorption on the lens surface was reduced greatly.

关键词： RGP contact lens PEGMA plasma graft polymerization protein adsorption

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Spatial correlation of irreversible displacement in oscillatory-sheared metallic glasses

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Chinese Physics B 2022年第8期31卷 547-551页

作者： shiheng Cui Huashan Liu Hailong Peng School of Materials Science and Engineering Central South UniversityChangsha 410083China

We report computer simulations on the oscillatory of Cu Zr metallic glasses at zero temperature with different shear amplitudes. In small system a homogenous shear deformation is found, while in large system an inhomogeneous shear deformation is found with a shear band formed. Concomitantly, spatial correlation of irreversible displacement exhibits an isotropic and exponential decay in the case of homogeneous deformation, whereas a mixed power-law and exponential decay in the case of anisotropic and inhomogeneous deformation. By projecting the azimuthal-dependent correlation function onto the spherical harmonics, we found a strong polar symmetry that accounts for the emerged shear band, and a weaker quadrupolar symmetry that accounts for the elastic filed generated by Eshelby inclusions. By this, we conclude that the anisotropy and decaying formula of the plastic correlation are dominated by the homogeneity or inhomogeneity for the deformation in the metallic glasses.

关键词： metallic glasses mechanical property molecular dynamics

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Molecular characterization of glutaredoxin 2 from Ostrinia furnacalis

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Integrative Zoology 2013年第1期8卷 30-38页

作者： shiheng AN Ying ZHANG Tiantian WANG Meihao LUO Chaohai LI College of Plant Protection Henan Agricultural UniversityZhengzhouChina College of Agronomy Henan Agricultural UniversityZhengzhouChina

Glutaredoxins(GRXs)play very important roles in maintaining intracellular redox *** the present study,the full-length cDNA sequence encoding GRX2,named OfurGRX2(GenBank accession ***393246),was obtained from Ostrinia furnacalis,using reverse transcription polymerase chain reaction and rapid amplification of cDNA *** analysis revealed that the open reading frame of OfurGRX2 consists of 351 nucleotides encoding 116 amino acid residues with a predicted molecular weight of 12.6 *** research revealed that OfurGRX2 shares a common active site,CPYC/CPFC,with other insect *** profiles revealed that OfurGRX2 is a ubiquitous gene expressed in insect heads,fat bodies,epidermises,mid guts and *** OfurGRX2 transcript peaked in 36-h larvae of 4th instars,and then suddenly declined in the molting *** treatment experiments revealed that 20-hydroxyecodyson(20e)significantly induces the expression of the OfurGRX2 transcript,whereas juvenile hormone(JH)counteracts 20e *** stress factors(including starvation,ultraviolet light,mechanical injury,Escherichia coli exposure,and high and low temperatures)dramatically induced OfurGRXGRX2 transcript expression,which confirmed for the first time that GRX2 play important roles in insecta during exposure to adverse environments.

关键词： gene regulation glutaredoxin Ostrinia furnacalis real-time polymerase chain reaction

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A deep Q-learning model for sequential task offloading in edge AI systems

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Intelligent and Converged Networks 2024年第3期5卷 207-221页

作者： Dong Liu shiheng Gu Xinyu Fan Xu Zheng Department of Computer Science and Engineering University of Electronic Science and Technology of ChinaChengdu 610000China Shenzhen Institute for Advanced Study University of Electronic Science and Technology of ChinaShenzhen 515100China Department of Information and Software Engineering University of Electronic Science and Technology of ChinaChengdu 610000China

Currently,edge Artificial Intelligence(AI)systems have significantly facilitated the functionalities of intelligent devices such as smartphones and smart cars,and supported diverse applications and *** fundamental supports come from continuous data analysis and computation over these *** the resource constraints of terminal devices,multi-layer edge artificial intelligence systems improve the overall computing power of the system by scheduling computing tasks to edge and cloud servers for *** efforts tend to ignore the nature of strong pipelined characteristics of processing tasks in edge AI systems,such as the encryption,decryption and consensus algorithm supporting the implementation of Blockchain ***,this paper proposes a new pipelined task scheduling algorithm(referred to as PTS-RDQN),which utilizes the system representation ability of deep reinforcement learning and integrates multiple dimensional information to achieve global task ***,a co-optimization strategy based on Rainbow Deep Q-Learning(RainbowDQN)is proposed to allocate computation tasks for mobile devices,edge and cloud servers,which is able to comprehensively consider the balance of task turnaround time,link quality,and other factors,thus effectively improving system performance and user *** addition,a task scheduling strategy based on PTS-RDQN is proposed,which is capable of realizing dynamic task allocation according to device *** results based on many simulation experiments show that the proposed method can effectively improve the resource utilization,and provide an effective task scheduling strategy for the edge computing system with cloud-edge-end architecture.

关键词： edge computing task scheduling reinforcement learning Rainbow Deep Q-Learning(RainbowDQN)

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A review of the literature on randomized controlled trials of acupuncture and moxibustion in the treatment of chronic prostatitis/chronic pelvic-pain syndrome within 2016-2021

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Journal of Traditional Chinese Medical Sciences 2022年第3期9卷 222-229页

作者： shiheng Wang Peijie Qin Fengxia Zhang Jianfeng Liu Institute for History of Medicine and Medical Literature China Academy of Chinese Medical SciencesBeijing100700China

Chronic prostatitis(CP)/chronic pelvic-pain syndrome(CPPS)is a common urinary-system disease with a high incidence in young and middle-aged men,seriously affecting patients'ability to work and their quality of life(Qo L).Western medicine(WM)has some limitations in treating CP/*** is an ancient Chinese medical method that is commonly used to treat this condition and has a relatively good effect on *** randomized controlled trials(RCTs)on this subject have been *** this study,we searched the China National Knowledge Infrastructure(CNKI),Chinese Science and Technology Periodical(VIP),Wanfang,Sino Med,and Pub Med databases for RCTs on acupuncture treatment of CP/CPPS in the last 5 years(2016/01/01-2021/12/01).In addition,we conducted analysis and research,aiming to summarize acupuncture treatment in CP/CPPS RCTs and the clinical efficacy,with the goal of providing clinical reference.A total of 466 related documents were retrieved in the search,and 62 articles were retained after *** obtained RCT information on acupuncture treatment of CP/*** results showed that several relevant clinical studies have been performed over the last 5 years and that acupuncture and moxibustion have better curative effect on CP/CPPS than *** to the small number of included studies,more experimental evidence is needed to verify the clinical efficacy of acupuncture.

关键词： Acupuncture Filament needle Warm-needle moxibustion Electroacupuncture Chronic prostatitis Clinical Mechanism Literature search

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