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A member of the ALOG gene family has a novel role in regulating nodulation in Lotus japonicus

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Journal of Integrative Plant Biology 2019年第4期61卷 463-477页

作者： Yawen Lei shihao su Liang He Xiaohe Hu Da Luo State Key Laboratory of Biocontrol and Guangdong Key Laboratory of Plant Resources School of Life Sciences Sun Yat-sen University Institute of Transformative Bio-Molecules (WPI-ITbM) Nagoya University

Legumes can control the number of symbiotic nodules that form on their roots, thus balancing nitrogen assimilation and energy consumption. Two major pathways participate in nodulation: the Nod factor(NF)signaling pathway which involves recognition of rhizobial bacteria by root cells and promotion of nodulation, and the autoregulation of nodulation(AON) pathway which involves long-distance negative feedback between roots and shoots. Although a handful of genes have a clear role in the maintenance of nodule number, additional unknown factors may also be involved in this process. Here, we identify a novel function for a Lotus japonicus ALOG(Arabidopsis LSH1 and Oryza G1) family member, LjALOG1,involved in positively regulating nodulation. LjALOG1 expression increased substantially after inoculation with rhizobia, with high levels of expression in whole nodule primordia and in the base of developing nodules. The ljalog1 mutants, which have an insertion of the LORE1 retroelement in LjALOG1, had significantly fewer nodules compared with wild type, along with increased expression of LjCLE-RS1(L. japonicus CLE Root Signal 1), which encodes a nodulation suppressor in the AON pathway. In summary,our findings identified a novel factor that participates in controlling nodulation, possibly by suppressing the AON pathway.

关键词： ALOG gene novel role Lotus

An AGAMOUS-like factor is associated with the origin of two domesticated varieties in Cymbidium sinense (Orchidaceae)

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Horticulture Research 2018年第1期5卷 323-331页

作者： shihao su Xiaoyu Shao Changfa Zhu Jiayin Xu Yuhuan Tang Da Luo Xia Huang State Key Laboratory of Biocontrol and Guangdong Key Laboratory of Plant Resources School of Life SciencesSun Yat-sen UniversityGuangzhou510275China Institute of Transformative Bio-Molecules(WPI-ITbM) Nagoya UniversityFuro-choChikusa-kuNagoya464-8601AichiJapan

Cymbidium has been artificially domesticated for centuries in Asia,which produced numerous cultivated varieties.Flowers with stamenoid tepals or those with multiple tepals have been found in different species of Cymbidium;however,the molecular basis controlling the formation of these phenotypes is still largely unknown.Previous work demonstrated that AGAMOUS/AG lineage MADS genes function in floral meristem determinacy as well as in reproductive organs development in both dicots and monocots,indicating a possible relationship with the origin of two flower varieties in Cymbidium.Here,we characterized and analyzed two AG lineage paralogues,CsAG1 and CsAG2,from Cymbidium sinense,both of which were highly expressed in the gynostemium column of a standard C.sinense.Interestingly,we detected ectopic expression of CsAG1 rather than CsAG2 in all floral organs of a stamenoid-tepal variety and significant down-regulation of CsAG1 in a variety with multiple tepals.Over-expression of CsAG1 in wild type Arabidopsis resulted in petal-to-stamen homeotic conversion,suggesting a conserved C-function of CsAG1 in the development of Cymbidium flower.Altogether,our results supported a hypothesis that disruption of a single AG-like factor would be associated with the formation of two domesticated varieties in C.sinense.

关键词： varieties domestica cultivated

Genetic control of compound leaf development in the mungbean(Vigna radiata L.)

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Horticulture Research 2019年第1期6卷 1571-1582页

作者： Keyuan Jiao Xin Li shihao su Wuxiu Guo Yafang Guo Yining Guan Zhubing Hu Zhenguo Shen Da Luo Institute of Traditional Chinese Medicine and Natural Products College of PharmacyJinan UniversityGuangzhouChina College of Life Sciences Laboratory Center of Life SciencesNanjing Agricultural UniversityNanjingChina Guangdong Key Laboratory of Plant Resources School of Life SciencesSun Yat-Sen UniversityGuangzhouChina Institute of Plant Stress Biology State Key Laboratory of Cotton BiologyDepartment of BiologyHenan UniversityKaifengChina

Many studies suggest that there are distinct regulatory processes controlling compound leaf development in different clades of legumes.Loss of function of the LEAFY(LFY)orthologs results in a reduction of leaf complexity to different degrees in inverted repeat-lacking clade(IRLC)and non-IRLC species.To further understand the role of LFY orthologs and the molecular mechanism in compound leaf development in non-IRLC plants,we studied leaf development in unifoliate leaf(un)mutant,a classical mutant of mungbean(Vigna radiata L.),which showed a complete conversion of compound leaves into simple leaves.Our analysis revealed that UN encoded the mungbean LFY ortholog(VrLFY)and played a significant role in leaf development.In situ RNA hybridization results showed that STM-like KNOXI genes were expressed in compound leaf primordia in mungbean.Furthermore,increased leaflet number in heptafoliate leaflets1(hel1)mutants was demonstrated to depend on the function of VrLFY and KNOXI genes in mungbean.Our results suggested that HEL1 is a key factor coordinating distinct processes in the control of compound leaf development in mungbean and its related non-IRLC legumes.

关键词： Vigna compound development

Medicinals with properties of warming Yang and tonifying Qi in terms of Traditional Chinese Medicine: their effects on left ventricular ejection fraction and aldosterone in rats with induced failure heart

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Journal of Traditional Chinese Medicine 2016年第6期36卷 789-793页

作者： Han Lihua Wang Zhentao Chai Songbo Xing Guohui Wang Hongxing Yuan Bin Li Zhiwei su shihao Department of Cardiology Traditional Chinese Medicine Hospital of Henan Province Department of Cardiology First Traditional Chinese Medicine Hospital of Luoyang

OBJECTIVE: To investigate on Left ventricular ejection fraction value and aldosterone of two medicinals of Traditional Chinese Medicine(TCM) with the properties of warming Yang and tonifying Qi in terms of TCM theory.METHODS: An animal model of coronary ligation of heart failure after myocardial infarction was employed to study the influence of these two kinds of drugs on three batches of rats. On the basis of the average score of left ventricle ejection fraction during the investigation, there were some different groups, including Wen Yang(the warming Yang)group, Yi Qi(tonifying Qi) group, Wen Yang and Yi Qi group, captopril group, digoxin group. In additional, an artificial operation group was set for compari-son. The systemic intervention using these medicinal and drugs was taken effects on the 2nd day after the operation of myocardial infraction(MI) with once a day. At week one, two, and four after the MI treatment, evaluated were EF values, and ferritin,angiotensin-II and aldosterone in the rats' plasma.REsuLTS: At week one and week two, the medicinal of Wen Yang, Yi Qi, Wen Yang pluse Yi Qi, and digoxin could improve left ventricular ejection fraction in rats with heart failure; Compared to the model group, captopril Left ventricular EF value increased, but there was not significant. At week four,heart failure and left ventricular EF values was improved in the intervention group and the other four captopril drug intervention. At week one in the rats with drug intervention, the medicinals of Yi Qi, Wen Yang plus Yi Qi, and captopril could inhibit activation in vivo hormone aldosterone in heart failure rats; aldosterone in Wen Yang group and digoxin group were not different from that in the model group at week two and four.CONCLUSION: Chinese medicinals with properties of Wen Yang, Yi Qi have significant effect on improving left ventricular EF in rats with heart failure; compared to Yi Qi medicinals, Wen Yang medicinals that inhibit the effectiveness of the time required for the activation of the role of aldo

关键词：心力衰竭醛固酮左心室益气大鼠分数中医气候变暖

GRK2 mediated degradation of SAV1 initiates hyperplasia of fibroblast-like synoviocytes in rheumatoid arthritis

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Acta Pharmaceutica Sinica B 2024年第3期14卷 1222-1240页

作者： Paipai Guo Ji Jiang Rui Chu Feng He Mingli Ge Ruhong Fang Qiuyun Guan Huijuan Cheng Chunru Jiang Tiantian su Zhenduo Zhu Hao Liu Wei Wei shihao Zhang Qingtong Wang Institute of Clinical Pharmacology Anhui Medical UniversityKey Laboratory of Anti-inflammatory and Immune MedicineMinistry of EducationCollaborative Innovation Center of Anti-inflammatory and Immune MedicinesHefei 230032China School of Pharmacy Bengbu Medical CollegeBengbu 233030China The Third Affiliated Hospital of Anhui Medical University(the First People’s Hospital of Hefei) Hefei 230061China

Hyperplasia and migration of fibroblast-like synoviocytes(FLSs)are the key drivers in the pathogenesis of rheumatoid arthritis(RA)and joint destruction.Abundant Yes-associated protein(YAP),which is a powerful transcription co-activator for proliferative genes,was observed in the nucleus of inflammatory FLSs with unknown upstream mechanisms.Using Gene Expression Omnibus database analysis,it was found that Salvador homolog-1(SAV1),the pivotal negative regulator of the Hippo-YAP pathway,was slightly downregulated in RA synovium.However,SAV1 protein expression is extremely reduced.subsequently,it was revealed that SAV1 is phosphorylated,ubiquitinated,and degraded by interacting with an important serine-threonine kinase,G protein-coupled receptor(GPCR)kinase 2(GRK2),which was predominately upregulated by GPCR activation induced by ligands such as prostaglandin E2(PGE2)in RA.This process further contributes to the decreased phosphorylation,nuclear translocation,and transcriptional potency of YAP,and leads to aberrant FLSs proliferation.Genetic depletion of GRK2 or inhibition of GRK2 by paroxetine rescued SAV1 expression and restored YAP phosphorylation and finally inhibited RA FLSs proliferation and migration.Similarly,paroxetine treatment effectively reduced the abnormal proliferation of FLSs in a rat model of collagen-induced arthritis which was accompanied by a significant improvement in clinical manifestations.Collectively,these results elucidate the significance of GRK2 regulation of Hippo-YAP signaling in FLSs proliferation and migration and the potential application of GRK2 inhibition in the treatment of FLSs-driven joint destruction in RA.

关键词： Rheumatoid arthritis Fibroblast-like synoviocytes G protein-coupled receptor kinase 2 Salvador homolog-1 Yes-associated protein

运动学对线与机械力学对线指导全膝关节置换对关节功能、疼痛及步态的影响

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中外医学研究 2022年第28期20卷 40-43页

作者：苏时豪化州市人民医院广东化州525100

目的:研究运动学对线与机械力学对线指导全膝关节置换对膝关节功能、疼痛及步态的影响。方法:回顾性分析化州市人民医院2018年1月-2021年3月收治的内翻性膝关节骨关节炎患者70例,按治疗差异分为两组,每组35例,其中对照组采用机械力学对... 详细信息

目的:研究运动学对线与机械力学对线指导全膝关节置换对膝关节功能、疼痛及步态的影响。方法:回顾性分析化州市人民医院2018年1月-2021年3月收治的内翻性膝关节骨关节炎患者70例,按治疗差异分为两组,每组35例,其中对照组采用机械力学对线指导手术,研究组采用运动学对线指导手术,观察两组手术前后美国特种外科医院(HSS)膝关节评分、疼痛评分、步速、步频、步幅、步态。结果:术前、术后1年两组HSS膝关节评分及疼痛评分比较,差异无统计学意义(P>0.05);术前、术后1年两组站立相及摆动相屈伸角度比较,差异无统计学意义(P>0.05);术前两组站立相及摆动相内外翻角度比较,差异无统计学意义(P>0.05),术后1年,研究组站立相内外翻角度大于对照组(P0.05);术前两组步速、步频、步幅比较,差异无统计学意义(P>0.05);术后1年,研究组步速、步幅均高于对照组,差异有统计学意义(P0.05);术后1年,两组膝关节生物力学各指标均大于术前,且研究组大于对照组,差异有统计学意义(P<0.05)。结论:全膝关节置换术经运动学对线与机械力学指导均能够改善患者术后膝关节功能与步态情况,但运动学对线对患者生物力学指标改善效果更加理想。

关键词：运动学对线机械力学全膝关节置换步态

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基于载能重离子的超薄薄膜纳米孔制备技术

科学通报 2023年第9期68卷 1090-1095页

作者：刘子荣李冬娜盛倩苏士皓朱昆薛建明王宇钢北京大学物理学院重离子物理研究所北京100871 北京大学物理学院核物理与核技术国家重点实验室北京100871 广东省新兴激光等离子体技术研究院广州510540 中国国际核聚变能源计划执行中心北京100038

超薄薄膜纳米孔材料由于其优异的物理和化学特性,在物理学、生物学、电子学和纳米科学等多学科领域应用潜力巨大.高效、便捷和可控的制孔方法对于获得高品质的纳米孔尤为重要,而载能重离子辐照不仅是一种强大的材料改性手段,也是一种独... 详细信息

超薄薄膜纳米孔材料由于其优异的物理和化学特性,在物理学、生物学、电子学和纳米科学等多学科领域应用潜力巨大.高效、便捷和可控的制孔方法对于获得高品质的纳米孔尤为重要,而载能重离子辐照不仅是一种强大的材料改性手段,也是一种独特的纳米孔制备方法.近年来,北京大学重离子物理研究所在基于载能重离子辐照技术研制超薄薄膜纳米孔技术方面的进展显著,所用材料包括传统有机高分子膜与新型二维材料.本文主要从核径迹刻蚀有机薄膜纳米孔的制备技术和二维材料纳米孔制备技术两方面展开介绍,分别阐述了这两种技术的制孔方法、改性方法和对应的纳米孔材料在能量转化、分子检测、离子运输、纳米流体等领域的应用,为进一步深入研究载能重离子制备其他人工固态纳米孔提供了参考.

关键词：重离子辐照核径迹纳米孔

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执行器部分失效的鲁棒模糊容错切换控制

南京理工大学学报 2024年第2期48卷 182-191,201页

作者：曹世豪施惠元苏成利李平辽宁石油化工大学信息与控制工程学院辽宁抚顺113001 东北大学流程工业综合自动化国家重点实验室辽宁沈阳110819 辽东学院信息工程学院辽宁丹东118001 辽宁科技大学电子与信息工程学院辽宁鞍山114051

针对一类具有不确定性、外部扰动和执行器部分失效的非线性工业过程,提出一种鲁棒模糊容错切换控制方法。首先,构建新型多自由度T-S模糊状态空间模型,在此基础上,结合执行器部分失效的情况设计控制律。其次,给出基于线性矩阵不等式(LMI... 详细信息

针对一类具有不确定性、外部扰动和执行器部分失效的非线性工业过程,提出一种鲁棒模糊容错切换控制方法。首先,构建新型多自由度T-S模糊状态空间模型,在此基础上,结合执行器部分失效的情况设计控制律。其次,给出基于线性矩阵不等式(LMI)的容错切换条件和系统稳定条件,并通过当前系统状态和稳定性条件求取其子模型的控制律及相应权值。最后,通过对连续搅拌釜式反应器的仿真研究来验证该文方法的有效性。

关键词：执行器部分失效容错切换 T-S模糊模型鲁棒控制预测控制

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钌碳耦合碱还原催化降解三倍体毛白杨

Chinese Journal of Catalysis 2022年第3期43卷 802-810页

作者：范亿薇李赫龙苏世浩陈金磊刘春泉王水众徐向亚宋国勇北京林业大学林木生物质化学北京市重点实验室北京100083 重庆文理学院化学与环境工程学院环境材料与修复技术重庆市重点实验室重庆402160 中国石油化工股份有限公司北京化工研究院北京100013

化石资源的过度消耗及因此产生的环境问题驱动了生物质资源各组分转化为燃料、化学品及材料的研究发展.木质素是自然界最丰富的可再生芳香碳氢资源,也是生物质中最难以实现高值化利用的组分.将木质素催化降解生成苯酚类单体化合物是其... 详细信息

化石资源的过度消耗及因此产生的环境问题驱动了生物质资源各组分转化为燃料、化学品及材料的研究发展.木质素是自然界最丰富的可再生芳香碳氢资源,也是生物质中最难以实现高值化利用的组分.将木质素催化降解生成苯酚类单体化合物是其高值化利用的关键,因此开发新型催化体系对于提高当前生物精炼产业经济效益具有重要意义.本文利用钌碳耦合碱催化体系,系统考察了三倍体毛白杨的直接催化还原反应.三倍体毛白杨原料中的木质素组分优先降解为高得率的苯酚单体化合物,同时保留碳水化合物(纤维素及半纤维素)组分.木质素二聚模型化合物研究结果表明,在钌碳催化体系中引入碳酸铯可以导致木质素β-O-4结构单元中C_(β)–O和C_(β)–C_(γ)键的断裂,从而生成含有乙基侧链的苯酚单体(C2).通过动力学及分离物种活性研究确定反应中观测到的二聚烯醚产物是木质素β-O-4降解的副产物.本文以木质素降解得到的苯酚类单体产率、选择性、木质素脱除率以及碳水化合物保留率等主要参数作为考察指标,系统研究了碱的种类和用量、反应温度、时间以及氢气压力等对三倍体毛白杨木屑催化还原降解的影响.结果表明,采用钌碳耦合碳酸铯体系,木质素生物大分子不仅降解为丙基、丙醇基取代的C3苯酚单体,同时生成部分C2苯酚产物,C2苯酚与C3苯酚的产物比值与碱的浓度呈现线性相关.通过钌碳耦合碳酸铯体系得到的碳水化合残渣具有较高的纤维素保留率.酶解对照实验发现,碱参与的催化还原降解反应得到的碳水化合物更易于酶解转化,这表明碱的加入可以有效地破解生物质中的抗降解屏障,从而提高酶解效率.本研究不仅提供了木质素高值化利用的方案,同时实现了生物质组分拆解、抗降解屏障破除及各组分的分级利用.

关键词：钌碳木质素还原催化降解三倍体毛白杨碱纤维素酶解