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Identification of two metallo- thionein isoforms by molecular cloning of their cDNAs in fresh-water fish,crucian carp (Carassius-cuvieri)

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Chinese Science Bulletin 2001年第1期46卷 53-57页

作者： REN Hongwei muto Norio Itoh Norio YU Meimin RU Binggeng Tanaka Keiichi National Laboratory of Protein Engineering College of Life SciencesPeking UniversityBeijing 100871China Department of Environmental Toxicology Faculty of Pharmaceutical SciencesOsaka University1-6 YamadaokaSuitaOsaka 565-0871Japan

Generally, there are two major isoforms of me-tallothionein (MT) in mammals. In this study two cDNAs of metallothionein, MT-A and MT-B, in a fresh-water fish, crucian carp (Carassius cuvieri), were cloned by the rapid amplification of cDNA ends (RACE). The homology of their reading frame is about 92.3%. The sequence analysis of both cDNAs gave the structures of coding regions corresponding to 60 amino acid residues, and the structures of complete 3’ -untranslated regions in which a significant difference in the size of their 3’-untranslated regions (130 bp for MT-A and 280 bp for MT-B) exists. The results of amino acid sequencing of both MT-1 and MT-2 purified by HPLC are identical to those deduced from MT cDNA genes, indicating that MT-1 is from MT-A gene and MT-2 is from MT-B gene respectively. No blocking in the N-terminal of MT-2 isoform was the first case found in vertebrates, most of which were blocked by acetylation. These results suggest that there were differential controls at the

关键词： metallothionein isoform carassius-cuvieri suppression RACE.

Ag/AAO纳米有序阵列复合结构等效光学参量的确定

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物理学报 2005年第12期54卷 5920-5925页

作者：王建王成伟李燕刘维民 Zaima shigeaki 西北师范大学物理与电子工程学院兰州730070 中国科学院兰州化学物理研究所固体润滑国家重点实验室兰州730070 名古屋大学结晶材料科学系

引入厚度偏差Δd,修正了薄膜透射率表达式.基于Ag/AAO纳米有序阵列复合结构实验透射光谱(500—2700nm)的两条极值包络线,定义了一个优化函数,结合最优化数值算法尝试确定具有较强吸收的Ag/AAO纳米有序阵列复合结构的等效光学参量.由此... 详细信息

引入厚度偏差Δd,修正了薄膜透射率表达式.基于Ag/AAO纳米有序阵列复合结构实验透射光谱(500—2700nm)的两条极值包络线,定义了一个优化函数,结合最优化数值算法尝试确定具有较强吸收的Ag/AAO纳米有序阵列复合结构的等效光学参量.由此计算了该结构的等效折射率n、等效消光系数k、平均等效厚度-d以及厚度偏差Δd.该方法对Ag/AAO纳米复合结构平均等效厚度的相对计算误差仅为0.3%,与实测厚度基本一致,且Ag/AAO纳米复合结构的模拟透射谱与实验透射光谱在500—2700nm波段范围内相符.这表明该计算方法可有效确定Ag/AAO纳米复合结构的等效光学参量,并与实验结果是自洽的.

关键词：薄膜光学光学参量纳米复合结构最优化算法

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构建公铁货物一体化运输系统的基础分析

交通运输系统工程与信息 2014年第6期14卷 1-7,29页

作者： LI Guoquan TAMURA Kazuki muto Masai OKUDA Daiki 铁路综合技术研究所亚洲成长研究所

建立多种运输方式一体化运输系统要考虑一些基础要素,如货物特点、托运人和承运人的评价,及其运输网的通道条件等.货物特点决定了相关潜在需求和不同交通方式组合形成多式一体化运输的可能性.托运人和承运人的评价会对运输方式的选择产... 详细信息

建立多种运输方式一体化运输系统要考虑一些基础要素,如货物特点、托运人和承运人的评价,及其运输网的通道条件等.货物特点决定了相关潜在需求和不同交通方式组合形成多式一体化运输的可能性.托运人和承运人的评价会对运输方式的选择产生重要影响.而运输网的通道条件要求各种运输方式改进相应的运输设备及服务.本文对这些要素进行对比分析,并讨论了形成公铁货物一体化运输系统的可能性及相关问题,最后,提出一些改进铁路货运服务的建议.

关键词：综合交通运输公铁一体化运输潜在需求对比评价托运人承运人

Uveitis and Intraocular inflammation in Asia

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Uveitis and Intraocular inflammation in Asia

第三届全球华人眼科学术大会暨中华医学会第十一届全国眼科学术大会

作者： shigeaki Ohno Department of Ophthalmology and Visual Sciences Hokkaido University Graduate School of Medicine SapporoHokkaidoJapan

Uveitis and intraocular inflammation (Ⅱ) sometimes run a chronic course with recurrence. Visual prognosis is poor if correct diagnosis and proper management are not done. It is well known that the etiology and cause differ from one population to another. Therefore, it is important for us Asian to know what are the major uveitis entities in Asian populations.

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New treatment of uveitis and intraocular inflammation

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New treatment of uveitis and intraocular inflammation

中华医学会第十二届全国眼科学术大会

作者： shigeaki OHNO Hokkaido University Graduate School of Medicine

Exact etiology and disease mechanisms of intraocular in- flammation such as uveitis or uveoretinitis are still obscure in most cases,and the visual prognosis of the patients is general- ly *** mainstay of the treatmen...

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Epigentic regulators for nuclear receptors

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Epigentic regulators for nuclear receptors

21st IUBMB and 12th FAOBMB International Congress of Biochemistry and Molecular Biology

作者： shigeaki Kato Institute of Molecular and Cellular Biosciences The University of Tokyo Japan ERA TO Japan Science and Technology Agency Japan

＜正＞Transcriptional controls by nuclear receptors have been recently shown to require chromatin reorganization and histone *** modifying enzymes other than histone acetylases/deacetylases have been identified as transcriptional co-regulators as multi-subunit complexes. Using a granulocytic differentiation system of HL60 cells induced by retinoic acid（RA） signaling through nuclear RA receptors（RARs）,a histone lysine

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“生物多样性的保护与扶贫:双赢策略的意义”的摘要(英文)

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“生物多样性的保护与扶贫:双赢策略的意义”的摘要(英文)

北京论坛（2010）文明的和谐与共同繁荣——为了我们共同的家园：责任与行动

作者： Tsukasa muto

The United Nations declared the year 2010 to be the International Year of *** environmental concerns such as pollution and climate change have been widely covered by the media and included in various political agendas,the significance of biodiversity and the danger of its decline have not been equally *** paper will briefly introduce the significance of biodiversity to global sustainability,and explore the relationship between biodiversity conservation and human poverty *** concept of biodiversity hotspot will be introduced,followed by four categories from which to view the relationship between biodiversity conservation and poverty *** paper will also critique a commonly implemented conservation strategy that uses Protected Areas,and will conclude by reviewing PES,or Payment for Ecosystem Services - a young yet hopeful strategy that integrates both biodiversity conservation and poverty reduction.

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Structural insights into the assembly of the 30S ribosomal subunit in vivo： functional role of S5 and location of the 17S rRNA precursor sequence

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Protein & Cell 2014年第5期5卷 394-407页

作者： Zhixiu Yang Qiang Guo Simon Goto Yuling Chen Ningning Li Kaige Yan Yixiao Zhang Akira muto Haiteng Deng Hyouta Himeno Jianlin Lei Ning Gao Ministry of Education Key Laboratory of Protein Sciences Center for Structural Biology School of Life Sciences Tsinghua University Beijing 100084 China Department of-Biochemistry and Molecular Biology Faculty of Agriculture and Life Science Hirosaki University Hirosaki 036-8561 Japan

The in vivo assembly of ribosomal subunits is a highly complex process, with a tight coordination between protein assembly and rRNA maturation events, such as folding and processing of rRNA precursors, as well as modifications of selected bases. In the cell, a large number of factors are required to ensure the efficiency and fidelity of subunit production. Here we characterize the immature 30S subunits accumulated in a factor-null Escherichia coil strain （ArsgAArbfA）. The immature 30S subunits isolated with varying salt concentrations in the buffer system show interesting differences on both protein composition and structure. Specifically, inter- mediates derived under the two contrasting salt condi- tions （high and low） likely reflect two distinctive assembly stages, the relatively early and late stages of the 3＇ domain assembly, respectively. Detailed structural analysis demonstrates a mechanistic coupling between the maturation of the 5＇ end of the 17S rRNA and the assembly of the 30S head domain, and attributes a unique role of S5 in coordinating these two events. Furthermore, our structural results likely reveal thelocation of the unprocessed terminal sequences of the 17S rRNA, and suggest that the maturation events of the 17S rRNA could be employed as quality control mech- anisms on subunit production and protein translation.

关键词： RsgA RbfA ribosome assembly cryo-EM,quantitative mass spectrometry

Actin filaments localization on the sorting endosomes

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Actin filaments localization on the sorting endosomes

Third Congress of the Asian-Pacific Organization for Cell Biology

作者： Hiroyuki Nakagawa shigeaki Miyamoto Dept.of Biochem. Eng. & Sci. Kyushu Inst. of Tech.IizukaFukuoka 820Japan

By immunofluorescence microscopic observation, monoclonal and polyclonal antibodies against a synthetic actin

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