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Chemokine-like factor 1 (CKLF1) is expressed in myocardial ischemia injury in vivo and in vitro

BIOCELL 2024年第6期48卷 981-990页

作者： JULING FENG HAODONG CHEN YANGBO LIU QIDI AI YANTAO YANG LEI ZHAO shifeng chu NAIHONG CHEN School of Pharmacy Department of PharmacyHunan Engineering Technology Center of Standardization and Function of Chinese Herbal Decoction PiecesHunan University of Chinese MedicineChangsha410208China Department of Pharmacology State Key Laboratory of Bioactive Substances and Functions of Natural MedicinesInstitute of Materia Medica&Neuroscience CenterChinese Academy of Medical Sciences and Peking Union Medical CollegeBeijing100050China Department of Diagnostics Research Lab of Translational Medicine Hengyang Medical CollegeUniversity of South ChinaHengyang421001China Department of Gastrointestinal Surgery The First Affiliated HospitalHengyang Medical CollegeUniversity of South ChinaHengyang421001China

Introduction:Chemokine-like factor 1(CKLF1)is a chemokine that is overexpressed in several *** previousﬁndings revealed a signiﬁcant increase in CKLF1 expression in the ischemic brain,suggesting its potential as a therapeutic target for ischemic ***:In this study,we examined the expression dynamics of CKLF1 in both in vivo and in vitro models of ischemic cardiac *** infarction(MI)was induced in vivo by ligation of the left anterior descending artery(LAD)of the rat *** levels of CKLF1,Creatine Kinase MB Isoenzyme(CK-MB),and Lactate dehydrogenase(LDH)in the serum were detected using Enzyme-linked immunosorbent assay(ELISA).The expression of CKLF1 in the infarcted area was detected by immunohistochemistry,immunoﬂuorescence,quantitative PCR(qPCR),and Western blotting(WB).H9C2 and AC16 cardiomyocytes cultured in vitro were subjected to oxygen and glucose deprivation(OGD).LDH was used to detect cell damage,and CKLF1 expression was detected by qPCR and ***:CKLF1 mRNA and protein expression were signiﬁcantly increased in h9c2 cells at 1.5 h and in AC16 cells at 4 h after *** serum CK-MB in rats increased signiﬁcantly on theﬁrst day after infarction,while the LDH concentration increased signiﬁcantly on the third day after ***1 blood levels signiﬁcantly increased on theﬁrst day following MI in ***1 expression notably increased in the infarct area on days 1,3,and 7 *** MI tissue,CKLF1 colocalizes with cardiomyocytes,macrophages,and ***:CKLF1 was substantially expressed during myocardial ischemia injury both in vivo and in vitro and was colocalized with macrophages and neutrophils,indicating that CKLF1 is expected to be a biomarker and a drug target for the treatment of myocardial infarction.

关键词： Chemokine-like factor 1 Overexpression Myocardial infarction

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Efficacy of Lidan Tang on high-fat-diet induced gallstone in mice and possible mechanism

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Journal of Traditional Chinese Medicine 2020年第4期40卷 584-592页

作者： Gao Yan Liu Caipin Li Juntong Zhai Yingying Lin Meiyu Wu Qinglin chu shifeng Zhang Zhao Li Jianping Zhou Xin Li Yueting Chen Naihong State Key Lab of Bioactive Substances and Functions of Natural Medicines Institute of Materia MedicaNeuroscience CenterChinese Academy of Medical Sciences and Peking Union Medical CollegeBeijing 100050China Department of Pharmacy Xuanwu Hospital of Capital Medical UniversityBeijing 100053China Department of Surgery Beijing Hospital of Integrated Traditional and Western MedicineBeijing 100039China Institute of Clinical Pharmacology Guangzhou University of Chinese MedicineGuangzhou 510405China College of Pharmacy Hunan University of Chinese MedicineChangsha 410208China

OBJECTIVE:To investigate efficacy of Lidan Tang(LDT)on gallstone induced by high fat diet in mice,and to study its underlying ***:Mice were fed with high fat diet every day and treated with LDT(9.01 times of human clinic dosage).Mice were randomly divided into 6 groups as control group,gallstone model group(high-fat diet),positive control ursodeoxycholic acid(UDCA)group(80 mg·kg^-1·d^-1,i.g.),LDT low dose group(6 kg/d,i.g.),LDT middle dose group(12 kg/d,i.g.),and LDT high dose group(24 kg/d,i.g.).The whole experiment was lasted for 4 *** levels of ALT,AST,LDH,CHO,HDL-C and LDL-C in serum were measured,the pathological sections were observed by hematoxylin-eosin staining,the activities of antioxidant enzymes were measured by kits,and the proteins related to oxidative stress and lipid transport were detected by Western blot ***:LDT could significantly reduce the contents of ALT and AST in serum and improve the pathological tissue of *** could significantly reduce the content of MDA and LPO,and increase the level of GSH and GSH-PX in liver *** data of Western blot showed that LDT had antioxidant effect promoting Keap1/Nrf2 pathway and regulated the process of lipid transport,which was statistically *** addition,LDT treatment inhibited the expression of ATP-binding cassette transports ABCG5/8 in liver,and reduced cholesterol transport from the hepatocytes to the ***:LDT has protective effect on gallstones induced by high fat diet in mice,which might be based on the protective effect on liver,including enhancing the antioxidant capacity of liver and reducing the production of lipid peroxides.

关键词： Cholelithiasis ATP-binding cassette transporters Oxidative stress Lipids Lidan Tang

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Antidepressant-like effect of active fraction of Polyrhachisvicina Roger in a rat depression model

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Journal of Traditional Chinese Medicine 2018年第1期38卷 12-21页

作者： Wei Guining chu shifeng Su Qibiao Su Hua Lin Meiyu He Fei Lu Wenjie Lu Guoshou Huang Zhoufeng Tan Xiao Lin Xiao Zeng Xianbiao Wei Baowei Chen Naihong Department of Pharmacology Guangxi Institute of Chinese Medicine and Pharmaceutical ScienceNanning 530022China State Key Laboratory of Bioactive Substances and Functions of Natural Medicines Institute of Materia&Neuroscience CenterChinese Academy of Medical Sciences and Peking Union Medical CollegeBeijing 100050China College of Health Science Guangdong Pharmaceutical UniversityGuangzhou 510006China College of Pharmacy Hunan University of Chinese MedicineChangsha 410208China

OBJECTIVE: To investigate the antidepressant-likeeffect of active fraction of Polyrhachis vicina Roger(AFPR) in a rat depression model, and to elucidate the underlying ***: AFPR was extracted with ethanol followed by petroleum ether. Its antidepressant-like effect was investigated in mice by tail suspension test(TST), forced swimming test(FST) and open field test(OPT). A repeated dose of reserpine(0.5 mg/kg, daily for 14 d) was used to establish a rat depression model. Fluoxetine was used as positive control agent. The effect of AFPR on reserpine-induced ptosis, hypothermia and akinesia, the levels of monoamines and their metabolites, and the activity of monoamine oxidase(MAO) in hippocampus and prefrontal cortex were ***: Administration of AFPR by gavage at 160 and 320 mg/kg significantly reduced the duration of immobility in the FST and TST, and did not affect locomotor activity in the OPT. In the reserpine-induced depression model, AFPR attenuated anhedonia, demonstrated by reversing hypothermia, akinesia and sucrose consumption. AFPR significantly increased the concentration of monoamines, including dopamine, serotonin, noradrenaline and ***: AFPR normalized the metabolism rates of noradrenaline, serotonin and dopamine,and the activity of MAO, which were altered by chronic reserpine exposure. The findings suggest that modulation of the monoaminergic neurotransmitter system likely underlies the antidepressant-like effect of AFPR.

关键词： Depression Polyrhachis vicina Roger Ether Ethanol Monoamine oxidase

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Simultaneous quantification of ginsenoside Rg1 and its metabolites by HPLC–MS/MS:Rg1 excretion in rat bile, urine and feces

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Acta Pharmaceutica Sinica B 2016年第6期6卷 593-599页

作者： Chiyu He Ru Feng Yupeng Sun shifeng chu Ji Chen Chao Ma Jie Fu Zhenxiong Zhao Min Huang Jiawen Shou Xiaoyang Li Yuzhu Wang Jinfeng Hu Yan Wang Juntian Zhang State Key Laboratory of Bioactive Substances and Functions of Natural Medicines Institute of Materia MedicaChinese Academy of Medical Sciences Beijing Analytical Application Center Shimadzu (China) Co. Ltd.

Ginsenoside Rg1(Rg1), the major effective component of ginseng, has been shown to have multiple bioactivities, but low oral bioavailability. The aim of this study was to develop a simple, sensitive and rapid high performance liquid chromatography–tandem mass spectrometry(LC–MS/MS) method, which could be used to validate and quantify the concentrations of Rg1 and its metabolites in Sprague-Dawley rat bile,urine, and feces after oral administration(25 mg/kg). Calibration curves offered satisfactory linearity(r40.995)within the determined ranges. Both intra-day and inter-day variances were less than 15%, and the accuracy was within 80–120%. The excretion recoveries of Rg1, ginsenoside Rh1(Rh1), and protopanaxatriol(Ppt) in bile,urine, and feces combined were all greater than 70%. The fecal excretion recoveries of Rg1, Rh1, and Ppt were40.11%, 22.19%, and 22.88%, respectively, whereas 6.88% of Rg1 and 0.09% of Rh1 were excreted in *** excretion accounted for only 0.04% of Rg1. In conclusion, the observed excretion profiles for Rg1 and its metabolites after oral administration are helpful for understanding the poor oral bioavailability of Rg1 and will aid further investigations of Rg1 as a pharmacologically active component.

关键词： Ginsenoside Rg1 Ginsenoside Rh1 Protopanaxatriol Excretion LC–MS/MS

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内镜下经下鼻道上颌道开窗术摘除上颌窦囊肿的临床分析

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中国社区医师 2015年第24期31卷 40-40,42页

作者：楚士凤沈丘县中医院耳鼻喉科 466300

目的:探讨分析应用内镜下经下鼻道上颌道开窗术对上颌窦囊肿患者进行治疗的临床效果。方法:收治上颌窦囊肿患者100例,回顾临床资料,观察统计临床治疗效果。结果:显效52例(52.0%),有效43例(43.0%),无效5例(5.0%),总有效率95.0%。结论:内... 详细信息

目的:探讨分析应用内镜下经下鼻道上颌道开窗术对上颌窦囊肿患者进行治疗的临床效果。方法:收治上颌窦囊肿患者100例,回顾临床资料,观察统计临床治疗效果。结果:显效52例(52.0%),有效43例(43.0%),无效5例(5.0%),总有效率95.0%。结论:内镜下经下鼻道上颌道开窗术对上颌窦囊肿患者进行治疗,其具有疗效确切、恢复快等临床特点,可以有效地治疗患者,使其达到临床治愈。

关键词：内镜经下鼻道上颌道开窗术上颌窦囊肿临床效果

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英语语法的象似性阐释

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中国石油大学学报（社会科学版） 2013年第2期29卷 90-94页

作者：褚世峰中国石油大学胜利学院外语系山东东营257061

象似性理论认为语言结构与人的经验结构之间存在照应性关系,语言结构不是任意的,而是有动因和理据可循的。象似性理论的这一观点可用于解释英语语法。通过对语法的距离象似性、顺序象似性及数量象似性加以阐释,已初步证明象似性理论对... 详细信息

象似性理论认为语言结构与人的经验结构之间存在照应性关系,语言结构不是任意的,而是有动因和理据可循的。象似性理论的这一观点可用于解释英语语法。通过对语法的距离象似性、顺序象似性及数量象似性加以阐释,已初步证明象似性理论对英语语法认知理据的理解富有指导意义。

关键词：象似性距离象似性顺序象似性数量象似性英语语法

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酸枣仁-茯苓-党参水提物对小鼠睡眠的改善作用及机制研究

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食品工业科技 2021年第11期42卷 300-308页

作者：徐飞飞田雅娟李钦青楚世峰贺文彬山西中医药大学中医脑病学山西省重点实验室山西太原030024 中国医学科学院药物研究所北京100050

目的:探究酸枣仁-茯苓-党参水提物(质量比1:1:1)对小鼠睡眠的改善作用,并探讨其潜在作用机制。方法:120只雄性昆明小鼠中选取60只随机分为6组,进行自主活动实验及戊巴比妥钠诱导睡眠实验,分为正常组、溶剂对照组、酸枣仁-茯苓-党参低、... 详细信息

目的:探究酸枣仁-茯苓-党参水提物(质量比1:1:1)对小鼠睡眠的改善作用,并探讨其潜在作用机制。方法:120只雄性昆明小鼠中选取60只随机分为6组,进行自主活动实验及戊巴比妥钠诱导睡眠实验,分为正常组、溶剂对照组、酸枣仁-茯苓-党参低、中、高剂量组(14、28、56 g/kg)、阳性药组(地西泮,2.5 mg/kg);另外60只小鼠进行对氯苯丙氨酸诱导失眠实验,分为正常组、模型组、酸枣仁-茯苓-党参低、中、高剂量组、阳性药组,每组10只,灌胃剂量为10 mL/(kg·d),不间断给药30 d。通过记录小鼠自主活动,分析水平得分与活动距离,评价酸枣仁-茯苓-党参水提物对正常小鼠及对对氯苯丙氨酸建立的失眠小鼠的的影响;通过戊巴比妥钠诱导睡眠实验,记录小鼠睡眠潜伏期和睡眠时间,评价酸枣仁-茯苓-党参水提物的促进睡眠及延长睡眠时间的作用。ELISA检测失眠实验中各组小鼠下丘脑、皮层中5-羟色胺(5-HT)及色氨酸羟化酶(TPH)的含量;小鼠海马、皮层中谷氨酸(Glu)及γ-氨基丁酸(GABA)的含量,探讨酸枣仁-茯苓-党参水提物相关作用机制。结果:酸枣仁-茯苓-党参水提物能够显著减少小鼠睡眠潜伏期,延长睡眠时间,降低失眠小鼠自主活动能力,减少正常小鼠水平得分与活动距离(P<0.05,P<0.01);高剂量酸枣仁-茯苓-党参水提物能够增强下丘脑5-HT合成关键酶TPH的活性,提高5-HT含量(P<0.05);能够调节海马Glu与GABA含量,维持Glu/GABA平衡(P<0.05)。结论:酸枣仁-茯苓-党参水提物对睡眠起到一定的改善作用,作用机制与调节小鼠脑内血清素系统和Glu/GABA稳态有关。

关键词：酸枣仁党参茯苓睡眠 5-羟色胺 γ-氨基丁酸

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神经血管单元结构和功能及其在脑缺血损伤发生机制中的作用

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中国药理学与毒理学杂志 2023年第12期37卷 885-894页

作者：马贺远李钦青楚世峰张钊贺文彬山西中医药大学分子中医药学国家国际联合研究中心中医脑病学山西省重点实验室山西太原030024 中国医学科学院药物研究所天然药物活性物质与功能国家重点实验室北京100050

近年来,从神经血管单元(NVU)这一整体性结构及其功能出发开展脑缺血发病机制的研究越来越受到人们的重视,NVU已经成为脑科学和脑重大疾病研究领域的热点之一。NVU主要组成细胞有神经元、星形胶质细胞、小胶质细胞和脑微血管内皮细胞等,... 详细信息

近年来,从神经血管单元(NVU)这一整体性结构及其功能出发开展脑缺血发病机制的研究越来越受到人们的重视,NVU已经成为脑科学和脑重大疾病研究领域的热点之一。NVU主要组成细胞有神经元、星形胶质细胞、小胶质细胞和脑微血管内皮细胞等,它们在结构上密切相关,在功能上共同维持脑稳态,在脑功能和脑缺血损伤中发挥重要作用。NVU损伤导致微血管及血脑屏障完整性受损、神经元细胞死亡、神经胶质反应、免疫细胞浸润,甚至组织损伤和脑水肿。本文以期阐明NVU的结构和功能及其在脑缺血损伤发生机制中的作用,为开展基于NVU结构和功能的脑缺血疾病防治药物研究提供新思路和新策略。

关键词：神经血管单元结构和功能脑缺血发病机制

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Anti-Tumor Effect of Tanshinone and Its Mechanism

Anti-Tumor Effect of Tanshinone and Its Mechanism

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第二届中国药理学会补益药药理专业委员会学术研讨会

作者： Zhao Zhang,shifeng chu,Juntian Zhang~*,Naihong Chen~* Institute of material medica,Chinese academy of medical sciences& Peking union medical college. Beijing,100050,China.

Tanshinones,which are abietane diterpenes,have been isolated from Salvia ***,tanshinones displayed significant antitumor activity,and high selectivity in tumor cell *** review will focus on the current researches of t...

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自我评价策略在大学英语写作教学中的应用研究

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中国石油大学胜利学院学报 2019年第4期33卷 59-62页

作者：庞小峰褚世峰中国石油大学胜利学院基础科学学院

自我评价不仅是一种评测工具,也是一种学习策略。写作中的自我评价不仅是对写作成果的评价,也包括对写作过程的评价,即学习者对写作前、写作时和写作后自己的行为进行监控、反思和调整。通过定量和定性研究相结合,对自我评价策略在大学... 详细信息

自我评价不仅是一种评测工具,也是一种学习策略。写作中的自我评价不仅是对写作成果的评价,也包括对写作过程的评价,即学习者对写作前、写作时和写作后自己的行为进行监控、反思和调整。通过定量和定性研究相结合,对自我评价策略在大学英语写作教学中的实施方法和效果进行探索研究,结果表明:自我评价策略可有效提高学生的英语写作兴趣、积极性和自信心,提高学生的英语写作水平。

关键词：自我评价大学英语写作教学

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