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Identification and mapping of a new powdery mildew resistance allele in the Chinese wheat landrace Hongyoumai

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Identification and mapping of a new powdery mildew resistanc...

江苏省遗传学会2017年学术研讨会——“技术创新与遗传学发展”

作者：付必胜 Zhiliang Zhang Qiaofeng Zhang Xiaoyou Wu 吴纪中 shibin cai Jiangsu Academy of Agricultural Sciences/The Jiangsu Provincial Platform for Conservation and Utilization of Agricultural Germplasm Nanjing Agricultural University

Powdery mildew(Pm) caused by Blumeria graminis f. sp. tritici(Bgt) is one of the world’s major wheat diseases and results in large grain yield losses. Discovery and utilization of Pm resistance genes constitutes the most common strategy for wheat Pm control. Hongyoumai, a wheat landrace from Henan Province in China, has excellent resistance to infection by Bgt. In order to identify the basis of such Pm resistance, a segregating population was submitted to genetic analysis, which showed that Pm resistance in Hongyoumai was conferred by a single recessive resistance gene. This gene was temporarily named pmHYM. Molecular marker analysis, chromosomal location, resistance spectrum analysis and an allelism test showed that pmHYM was located on the long arm of chromosome 7 B(7 BL), most likely representing a new recessive resistance gene allelic with Pm5 e and mlXBD. By using 90 kb single nucleotide polymorphisms sequences(SNP) in the BLASTn analysis against the wheat 7 BL genome sequence, twelve new simple sequence repeat(SSR) markers linked with pmHYM were developed to map pmHYM co-segregating with the marker Xmp1207 and between markers Xmp925 and Xmp1158, at genetic distances of 2.8 c M and 2.7 cM, respectively. In addition, physical mapping of the markers linked with pmHYM using Chinese Spring deletion lines indicated a location in the 0.86–1.00 bin of 7 BL.

关键词： powdery mildew Chinese wheat landrace molecular mapping SSR markers pmHYM

Identification and mapping of a new powdery mildew resistance allele in the Chinese wheat landrace Hongyoumai

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Identification and mapping of a new powdery mildew resistanc...

第八届全国小麦基因组学及分子育种大会

作者： Bisheng Fu Zhiliang Zhang Qiaofeng Zhang Xiaoyou Wu Jizhong Wu shibin cai Institute of Crop Germplasm and Biotechnology Jiangsu Academy of Agricultural Sciences/Jiangsu Province Platform for Conservation and Utilization of Agricultural Germplasm Nanjing Agricultural University

Powdery mildew(Pm) caused by Blumeria graminis f ***(Bgt) is one of the world’s major wheat diseases and results in large grain yield *** discovery and utilization of Pm resistance genes is the most common strategy for wheat Pm ***,a wheat landrace from Henan Province in China,is an excellent source of resistance against *** order to identify its Pm inheritance,we set up a segregating population and carried out genetic analysis,which showed that a single recessive resistance gene confers Pm resistance in *** have temporarily named this gene *** marker analysis,chromosomal location,resistance spectrum analysis,and an allehsm test showed that pmHYM is tocated on the long arm of chromosome 7 B(7 BL) and is most likely a new recessive resistance gene allelic with Pm5 e and *** using 90 Kb SNP sequence in the BLASTn analysis against the wheat 7 BL genome sequence,twelve new simple sequence repeat(SSR) markers linked with pmHYM were developed to map pmHYM co-segregating with marker Xmp1207 and between markers Xmp925 and Xmp1158,at genetic distances of 2.8 cM and 2.7 cM,*** addition,physical mapping of the markers linked with pmHYM using Chinese Spring deletion lines indicated that it resides in the 0.86-1.00 bin of 7 BL.

关键词： Powdery mildew Molecular mapping SSR markers pmHYM

Genome-wide mapping for low kernel polyphenol oxidase loci in wheat germplasm "IDO580-21"

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Genome-wide mapping for low kernel polyphenol oxidase loci i...

第十届全国小麦基因组学及分子育种大会

作者： Jin cai shibin cai Jishu Yang Qiaofeng Zhang Bisheng Fu Wei Guo Ying Liu Wenling Zhai Xiaoyou Wu Jizhong Wu Institute of Germplasm Resources and Biotechnology Jiangsu Academy of Agricultural Sciences College of Agronomy Nanjing Agricultural University

Whiteness is one of the most important quality traits for wheat flour and its *** oxidase(PPO) is a major factor that causes browning of wheat flour and its ***,to develop new variety with blight whiteness,we have to screen elite germplasms with low PPO activity and to explore its new genes of low PPO ***580-21 is a hard spring wheat showed near null levels of seed PPO *** map the quantitative trait loci(QTL) for low PPO activity and identify markers tightly linked to them in IDO580-21,a population of 111 recombinant inbred lines(RILs) from a cross between IDO580-21 and Yangmail58,a cultivar with high PPO activity,was genotyped using simple sequence repeats(SSRs) and single-nucleotide polymorphisms(SNPs) derived from 55 K SNP *** activity in the RILs was determined in three field experiments and one greenhouse *** QTL for low PPO activity were mapped on six chromosomes(2 A,2 D,5 D,3 A,6 A,7 B).Among them,the QTL mapped on 5 DL(QPpoDO580 D) was a novel QTL with large and stable effect,which explained 7.6～26.2% of phenotypic variations across the four ***580 5 D was mapped between SNP markers AX-SNP1799 and AX-SNP4585 with the genetic distance of 2.2 cM,and the physical distance of 62 *** SNPs linked to the QTLs on chromosomes 2 A,2 D,and 5 D were converted into KBioscience competitive allele-specific PCR(KASP) assays,which can be used for marker-assisted selection(MAS)

关键词： QTL for low polyphenol oxidase activity Triticum aestivum L. 55K SNP chip KASP

DETERMINATION OF STRUCTURAL RIGIDITY CENTER BY COHERENCE AREA

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DETERMINATION OF STRUCTURAL RIGIDITY CENTER BY COHERENCE ARE...

2001亚太振动会议

作者： XU shibin cai Xianhui SHI Zan Department of Engineering Mechanics Dalian University of Technology Dalian 116023 P. R. China

＜正＞ A reliable method was put forward to estimate rigiditycenter of standing buildings from vibration recordings. Itwas based on the characteristic of the center that thetranslational motion at that position is minimally coheredto the torsional motion during vibration. Defining thecoherence area as integration of coherence function oftorsional motion and translational motion in frequencyfield and calculating of the area at any positions in floorslabs, the rigidity center was determined at the positionwhere coherence area reaches minimum. An example waspresented in this paper and the validity of this methodwas proved.

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小麦孕穗期水渍和高温对叶片枯衰的影响

江苏农业学报 1987年第3期 45-47页

作者：蔡士宾曹暘江苏省农业科学院

材料和方法选用耐湿性强弱不等的水里占、农林46、宁8319,扬麦5号、Alondra“s”和鄂麦6号六个品种为供试材料。种子浸泡24小时露白后,播于底部钻孔的塑料盆钵内。盆口直径20厘米,盆底直径15厘米,高17厘米。出苗后每盆留苗3株,拔节时,... 详细信息

材料和方法选用耐湿性强弱不等的水里占、农林46、宁8319,扬麦5号、Alondra“s”和鄂麦6号六个品种为供试材料。种子浸泡24小时露白后,播于底部钻孔的塑料盆钵内。盆口直径20厘米,盆底直径15厘米,高17厘米。出苗后每盆留苗3株,拔节时,每品种挑选生长均匀一致的16盆移入人工气候箱培养,拔节后15天开始进行处理。试验采用裂裂区设计。主处理为温度,一个水平为20/12℃(昼/夜),午间2小时25℃;另一水平为25/15℃,午间2小时30℃。副处理为土壤湿度,一个水平为过湿处理,盆钵浸于盛水的塑料周转箱内,使土表渗水;另一水平为正常浇水供生长所需。试验4次重复。人工气候箱的光强为3万勒克斯,日照时数12小时,相对湿度60—80％。在抽穗期结束时,用目测法调查了每盆(3株)的主茎绿叶片数,不足一叶的叶片用小数表示。

关键词：小麦耐湿早衰

江苏省农业种质资源平台库(圃)管理机制研究

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农业科技管理 2017年第3期36卷 41-44页

作者：杨欣颜伟朱银沈奇蔡士宾江苏省农业科学院种质资源与生物技术研究所南京210014 江苏省农业种质资源保护与利用平台南京210014

文章阐述了江苏省农业种质资源平台库(圃)建设运行现状,针对平台库(圃)管理工作中存在的问题,提出建立库(圃)进出机制、制订分类管理方案、构建库(圃)运行服务绩效评价体系等建议,以期通过管理机制的创新进一步规范库(圃)的建设和运行,... 详细信息

文章阐述了江苏省农业种质资源平台库(圃)建设运行现状,针对平台库(圃)管理工作中存在的问题,提出建立库(圃)进出机制、制订分类管理方案、构建库(圃)运行服务绩效评价体系等建议,以期通过管理机制的创新进一步规范库(圃)的建设和运行,促进江苏省农业种质资源的开放共享。

关键词：江苏省农业种质资源平台库(圃)管理运行机制研究

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10个小麦品种耐光氧化测定

上海农学院学报 1998年第3期16卷 189-192页

作者：方先文曹旸蔡士宾江苏省农业科学院粮食作物所南京210014

将离体的小麦叶片用透明的玻璃压板压入水中,置于自然光下,人为造成低氧、低二氧化碳的逆境,7d后通过测定叶绿素含量,比较发现:Pato和宁麦 3号的单位重量的叶片内叶绿素含量下降量较小;白江西和水里占下降量较大。以单位重量的叶片内叶... 详细信息

将离体的小麦叶片用透明的玻璃压板压入水中,置于自然光下,人为造成低氧、低二氧化碳的逆境,7d后通过测定叶绿素含量,比较发现:Pato和宁麦 3号的单位重量的叶片内叶绿素含量下降量较小;白江西和水里占下降量较大。以单位重量的叶片内叶绿素含量下降量为选择指标,筛选出的耐光氧化小麦材料与目测、聚类分析结果是一致的。

关键词：小麦种质耐乐氧化筛选品种

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江苏省农业种质资源库管系统设计与构建

农业网络信息 2010年第11期 44-47页

作者：杨欣颜伟许大光蔡士宾王才林江苏省农业科学院粮食作物研究所江苏省农业种质资源保护与利用研究中心南京210014

以江苏省农业种质资源冷藏库为基础,从目前庞大的种质资源保存规模入手,设计构建了"江苏省农业种质资源库管系统",用于受理全省农业种质资源入库保存申请,管理种质资源实物出入库记录,保存入库种质的应用反馈信息。介绍了系统功能设计... 详细信息

以江苏省农业种质资源冷藏库为基础,从目前庞大的种质资源保存规模入手,设计构建了"江苏省农业种质资源库管系统",用于受理全省农业种质资源入库保存申请,管理种质资源实物出入库记录,保存入库种质的应用反馈信息。介绍了系统功能设计、系统物理及逻辑架构以及系统数据模型等,详细描述了系统关键业务流程。

关键词：种质资源库管系统系统功能数据模型业务流程

Effects of plant height vary with type Ⅰ and type Ⅱ resistances of Fusarium head blight in wheat

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Effects of plant height vary with type Ⅰ and type Ⅱ resist...

江苏省遗传学会第八届会员代表大会暨学术研讨会

作者： Wei Yan a,b,Haobing Li b,shibin cai a,Hongxiang Ma c and Chunji Liu b* a Institute of Food Crops,Jiangsu Academy of Agricultural Sciences,Nanjing,210014,China b CSIRO Plant Industry,Queensland Bioscience Precinct,St Lucia,QLD 4067,Australia c Institute of Biotechnology,Jiangsu Academy of Agricultural Sciences,Nanjing 210014,China

＜正＞By carefully separating the typeⅠand typeⅡresistances,possible effects of plant height on Fusarium head blight（FHB） were assessed using near-isogenic lines（NILs） for several different reduced height （Rht） genes in *** isolines all gave better typeⅠresistance than their respective dwarf counterparts when assessed at their natural *** differences were largely disappeared when the dwarf isolines were physically elevated so that their spikes were positioned at the same height of their respective tall counterparts. The effects of plant height on typeⅡseverity was less *** those of NIL pairs which showed significant differences,it’s the dwarf isolines which gave better *** the Rht genes involved in these NILs locate at different genomic regions,the different FHB severities between the dwarf and tall isolines are unlikely due to linkages between each of the different Rht loci with a beneficial or a deleterious gene affecting typeⅠor typeⅡ***,the different FHB severities must be due to direct or indirect effects of height difference per se and microclimate seems a significant contributing factor to the better typeⅠresistance of the tall *** caution should be exercised when attempting to exploit any of those ’FHB resistant loci’ co-located with Rht genes.

关键词： Fusarium head blight reduced height gene near-isogenic lines