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Anti-adipogenic effects of extracts of Ficus deitoidea var. deltoidea and var. angustifolia on 3T3-L1 adipocytes

Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2014年第3期15卷 295-302页

作者： shiau mei woon Yew Wei SENG Anna Pick Kiong LING Soi Moi CHYE Rhun Yian KOH School of Pharmacy International Medical University Bukit Jalil 57000 Kuala Lumpur Malaysia Department of Human Biology International Medical University Bukit Jalil 57000 Kuala Lumpur Malaysia

Objective： This study examined the anti-adipogenic effects of extracts of Ficus deltoidea var. deltoidia and var. angustifolia, a natural slimming aid, on 3T3-L1 adipocytes. Methods： Methanol and water extracts of leaves of the F. deltoidea varieties were analyzed to determine their total flavonoid content （TFC） and total phenolic content （TPC）, respectively. The study was initiated by determining the maximum non-toxic dose （MNTD） of the methanol and water extracts for 3T3-L1 preadipocytes. Possible anti-adipogenic effects were then examined by treating 2-d post confluent 3T3-L1 preadipocytes with either methanol extract or water extract at MNTD and half MNTD （1/2MNTD）, after which the preadipocytces were induced to form mature adipocytes. Visualisation and quantification of lipid content in mature adipocytes were carried out through oil red O staining and measurement of optical density （OD） at 520 nm, respec- tively. Results： The TFCs of the methanol extracts were 1.36 and 1.97 g quercetin equivalents （QE）/100 g dry weight （DW）, while the TPCs of the water extracts were 5.61 and 2.73 g gallic acid equivalents （GAE）/100 g DW for var. deltoidea and var. angustilofia, respectively. The MNTDs determined for methanol and water extracts were （300.0±28.3） and （225.0±21.2） μg/ml, respectively, for var. deltoidea, while much lower MNTDs [（60.0±2.0） IJg/ml for methanol extracts and （8.0＋1.0） μg/ml for water extracts] were recorded for var. angustifolia. Studies revealed that the methanol extracts of both varieties and the water extracts of var. angustifolia at either MNTD or 1/2MNTD significantly inhibited the maturation of preadipocytes. Conclusions： The inhibition of the formation of mature adipocytes indicated that leaf extracts of F. deltoidea could have potential anti-obesity effects.

关键词： Ad Dogenesis Ficus deltoidea Flavonoids Natural products Obesity

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Recent Advances in Directed Assembly of Nanowires or Nanotubes

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Nano-Micro Letters 2012年第3期4卷 142-153页

作者： mei Liu Zhizheng Wu woon Ming Lau Jun Yang School of Mechatronics Engineering and Automation Shanghai University State Key Laboratory of Transducer Technology Shanghai Institute of Microsystem and Information TechnologyChinese Academy of Sciences Green Energy and Green Manufacturing Technology R&D Center Department of Mechanical and Materials Engineering the University of West ern Ontario

Nanowires and nanotubes of diverse material compositions,properties and/or functions have been produced or fabricated through various bottom-up or top-down *** nanowires or nanotubes have also been utilized as potential building blocks for functional *** key for the integration of those nanowire or nanotube based devices is to assemble these one dimensional nanomaterials to specific locations using techniques that are highly controllable and *** such techniques should enable assembly of highly uniform nanowire/nanotube arrays with precise control of density,location,dimension or even material types of nanowires/*** assembly techniques are being developed that can quickly align and assemble large quantities of one type or multiple types of nanowires through parallel processes,including flow-assisted alignment,Langmuir-Blodgett assembly,bubble-blown technique,electric/magnetic-field directed assembly,contact/roll printing,knocking-down,etc..With these assembling techniques,applications of nanowire/nanotube based devices such as flexible electronics and sensors have been *** paper delivers an overall review of directed nanowire/nanotube assembling approaches and analyzes advantages and limitations of each *** future research directions have also been discussed.

关键词： Nanowire Nanotube Assembly Shear force Contact printing Dielectrophoresis Microfluidics Nanosystems Nanodevices

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Feasibility of enhanced phytoextraction of Zn contaminated soil with Zn mobilizing and plant growth promoting endophytic bacteria

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Transactions of Nonferrous Metals Society of China 2013年第8期23卷 2389-2396页

作者：龙新宪陈雪梅黄焕忠卫泽斌吴启堂华南农业大学资源环境学院广州510642 华南农业大学广东省教育厅土壤环境与废物利用重点实验室广州510642 香港浸会大学生物系

Three bacterial endophytes of Sedum alfredii, VI8L2, II8L4 and VI8R2, were examined for promoting soil Zn bioavailability and Zn accumulation in S. alfredii. Results showed that three strains were re-introduced into S. alfredii rhizosphere soils under Zn stress and resulted in better plant growth, as roots biomass increased from 80% to 525% and shoot biomass from 11% to 47% compared with the uninoculated ones. Strains IVsLz, II8L4 and IVsR2 significantly increased shoot and root Zn concentrations in the ZnCO3 contaminated soil. Inoculation with strain IVsL2 resulted in 44% and 39% higher shoot and root Zn concentrations, while strain IV8R2 significantly decreased shoot Zn concentration in the Zn3（PO4）2 contaminated soils. In the aged contaminated soil, isolates IVsL2, IIsL4 and IVsR2 significantly increased root Zn concentration, but decreased shoot Zn concentration of Sedum alfredii. It suggested that endophytes might be used for enhancing phytoextraction efficiency.

关键词： endophytic bacteria Zn bioavailability phytoremediation Sedum alfredii

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