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基于AMPK/SIRT1/SREBP1信号通路探究膜肾颗粒对膜性肾病大鼠蛋白尿的治疗作用

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中草药 2024年第2期55卷 509-515页

作者：王含香林威崔师妍徐荣佳徐鹏昊黄诗琦李雨琛戴欣晴姜晨天津中医药大学第一附属医院国家中医针灸临床医学研究中心天津300381 厦门市中医院福建厦门361001

目的基于单磷酸腺苷活化的蛋白激酶(adenosine monophosphate-activated protein kinase,AMPK)/沉默信息调节因子1(silent information regulator 1,SIRT1)/固醇调节元件结合蛋白1(sterol regulatory element-binding protein 1,SREBP1)信号通路观察膜肾颗粒对膜性肾病大鼠蛋白尿的影响及作用机制。方法 80只SD大鼠随机挑选15只为空白组,其余大鼠利用改良Border法建立膜性肾病大鼠模型,造模大鼠随机分为模型组、环孢素A(cyclosporin A,CsA)组和膜肾颗粒低、高剂量(1.85、7.38 g/kg)组。观察大鼠一般状态,采用双缩脲比色法检测大鼠造模前、造模后、给药结束后24 h尿蛋白定量(urinary protein quantity,UTP);使用全自动生化仪检测大鼠尿素氮(blood urea nitrogen,BUN)、血肌酐(serum creatinine,Scr)、总胆固醇(total cholesterol,TC)、三酰甘油(triglyceride,TG)水平;采用苏木素-伊红(hematoxylin-eosin,HE)、基底膜六胺银染色(periodic acid-silver methenamine,PASM)染色观察肾脏病理学变化;采用Western blotting检测大鼠肾组织Nephrin、AMPK/SIRT1/SREBP1通路相关蛋白表达。结果各组BUN、Scr水平无明显差异。与空白组比较,模型组大鼠24 h-UTP、TC、TG水平明显升高(P<0.01),肾组织病理改变明显,肾组织Nephrin、p-AMPKα/AMPKα和SIRT1表达显著降低(P<0.01),SREBP1蛋白表达水平显著升高(P<0.01)。与模型组比较,各给药组24 h-UTP、TC、TG水平明显降低(P<0.01),肾组织病理改变减轻,Nephrin、p-AMPKα/AMPKα、SIRT1蛋白表达水平明显升高(P<0.05、0.01),SREBP1蛋白表达水平显著降低(P<0.01)。结论膜肾颗粒可以显著减少膜性肾病大鼠蛋白尿水平,可能与上调肾组织AMPKα/SIRT1信号通路表达,减少SREBP1合成,改善脂代谢重编程有关。

关键词：膜性肾病脂代谢膜肾方膜肾颗粒 AMPK/SIRT1/SREBP1通路

新型QuEChERS方法结合高效液相色谱串联四极杆飞行时间质谱法快速测定鱼肉中14种激素

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中国渔业质量与标准 2016年第5期6卷 52-59页

作者：李诗言王扬周凡郑重莺柯庆青王鼎南贝亦江浙江省水产质量检测中心浙江杭州310023

建立了一种新型QuEChERS前处理方法,结合高效液相色谱-四极杆飞行时间质谱（LC-Q-TOF/MS）技术对鱼肉中14种激素进行快速筛查测定。样品加入水分散基质后采用乙腈提取,提取液经增强型基质去脂吸附剂（EMRlipid）脱脂,盐析后采用PSA吸附... 详细信息

建立了一种新型QuEChERS前处理方法,结合高效液相色谱-四极杆飞行时间质谱（LC-Q-TOF/MS）技术对鱼肉中14种激素进行快速筛查测定。样品加入水分散基质后采用乙腈提取,提取液经增强型基质去脂吸附剂（EMRlipid）脱脂,盐析后采用PSA吸附剂进一步净化并浓缩,然后用LC-Q-TOF/MS方法检测分析。所有药物在线性范围内线性良好,定量限范围为1.4-6.7μg/kg。3个添加水平（n=6）下草鱼基质的平均回收率为75.4%-103.6%,相对标准偏差（RSD）为2.4%-9.5%;大黄鱼基质的平均回收率为73.4%-101.5%,相对标准偏差（RSD）为2.4%-9.1%。该方法利用精确质量数匹配和自建标准谱库检索,实现快速筛查,并使用多级特征碎片离子进行确证,具有操作简便、结果准确等优点,适用于不同鱼肉基质,可作为鱼肉中14种激素类药物的高通量筛查和确认检测方法。本研究为监测鱼肉中激素类药物的风险提供了有效的技术手段。

关键词：高效液相色谱串联四极杆飞行时间质谱激素 QuEChERS 草鱼大黄鱼筛查参数

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电缆隧道建设中的地质风险

岩土工程技术 2024年第4期38卷 477-481页

作者：郝兵刘莹光李从昀刘世岩北京电力经济技术研究院有限公司北京100055

地质条件可能引发的工程风险主要包括安全风险、质量风险、工期风险、环境风险和投资风险。岩土工程勘察文件应重点关注安全风险和质量风险。从电缆隧道的施工工法和工序出发,分析竖井支护开挖、竖井地下水控制、隧道主体施工过程中的... 详细信息

地质条件可能引发的工程风险主要包括安全风险、质量风险、工期风险、环境风险和投资风险。岩土工程勘察文件应重点关注安全风险和质量风险。从电缆隧道的施工工法和工序出发,分析竖井支护开挖、竖井地下水控制、隧道主体施工过程中的地质条件可能带来的风险,根据工程地质条件进行地质风险识别。建议参考轨道交通风险控制经验分层级建立地质风险清单,制定风险控制措施。

关键词：地质条件工法地质风险识别

同方期刊数据库博看期刊评论

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传感器技术在浮动曲线测试中的应用

测试技术学报 2004年第Z1期18卷 201-204页

作者：蒋有才赵玉龙张彦斌李世炎韩晓光军械工程学院石家庄050003 军械工程学院石家庄050003 军械工程学院石家庄050003 济南军区修理所济南250022 南京军区修理所南京210000

本文通过分析某型高炮浮动机在射击时的动作特点及其对射击精度的影响,阐述了浮动曲线测试的重要性.提出利用位移传感器测试浮动曲线的设计方案,并采用信号采集与放大、数字滤波与处理和温度补偿等技术,成功实现了浮动机射击曲线的自动... 详细信息

本文通过分析某型高炮浮动机在射击时的动作特点及其对射击精度的影响,阐述了浮动曲线测试的重要性.提出利用位移传感器测试浮动曲线的设计方案,并采用信号采集与放大、数字滤波与处理和温度补偿等技术,成功实现了浮动机射击曲线的自动测试和调整部位判断,并能正确评价浮动机的整体工作性能,为浮动机的精确调试提供可靠的依据.

关键词：传感器浮动曲线位移信号采集

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熟化对面带质地特性的影响

农业工程 2020年第2期10卷 55-58页

作者：刘全李世岩刘淑一徐斌梁晓军王金永中国农业机械化科学研究院北京100083 江苏大学食品与生物工程学院江苏镇江212000

研究在不同熟化时间和温度条件下,面带沿压延方向和垂直压延方向上的拉伸和压缩特性,以确定面带熟化对面带质地特性的影响。通过对熟化时间为0、15、30、45、60和90min及熟化温度为25、35和45℃的面带分别进行拉伸和压缩试验。结果表明... 详细信息

研究在不同熟化时间和温度条件下,面带沿压延方向和垂直压延方向上的拉伸和压缩特性,以确定面带熟化对面带质地特性的影响。通过对熟化时间为0、15、30、45、60和90min及熟化温度为25、35和45℃的面带分别进行拉伸和压缩试验。结果表明,在熟化温度25℃的条件下,随着面带熟化时间延长,面带的抗延伸位移增加,抗拉伸力减小;在熟化时间30 min的条件下,随着面带熟化温度升高,面带的抗拉伸力减小,面带抗延伸位移先增大再减小,面带最大黏附力显著增加,其中熟化温度45℃时,面带的横向抗延伸位移显著减小,面带最大黏附力比25℃时增加2倍多。综上,在熟化温度35℃的条件下熟化30 min可以达到明显熟化效果;试验得到了面带熟化时间及温度对面带质地特性的影响规律。

关键词：面带熟化熟化时间熟化温度拉伸特性压缩特性

两种不同基质的垂直潜流人工湿地对尾水的处理效果研究

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环境污染与防治 2024年第4期46卷 484-489页

作者：孙健李卿蔡世颜夏娜汪博飞万年红程禹皓陈骞中国市政工程中南设计研究总院有限公司湖北武汉430010 中信清水入江(武汉)投资建设有限公司湖北武汉430200

为提高人工湿地对污水处理厂尾水的处理效率,采用以黄铁矿和砾石为基质的两种垂直潜流人工湿地处理武汉某污水处理厂尾水,研究了不同水力负荷下人工湿地去除污染物的效果,并分析了基质的微生物群落结构。结果表明,在低水力负荷(0.4~0.7 ... 详细信息

为提高人工湿地对污水处理厂尾水的处理效率,采用以黄铁矿和砾石为基质的两种垂直潜流人工湿地处理武汉某污水处理厂尾水,研究了不同水力负荷下人工湿地去除污染物的效果,并分析了基质的微生物群落结构。结果表明,在低水力负荷(0.4~0.7 m^(3)/(m^(2)·d))下,两种人工湿地对尾水化学需氧量(COD)、总氮(TN)、NO_(3)^(-)和总磷(TP)具有较好的去除效果;在高水力负荷(1.0~1.3 m^(3)/(m^(2)·d))下,对氨氮去除效果较好。黄铁矿人工湿地对尾水COD、TN、NO_(3)^(-)和TP的去除效果好于砾石,分析基质微生物发现,黄铁矿人工湿地中反硝化脱氮菌属主要为硫杆菌属(Thiobacillus)和硫氧化菌属(Sulfurifustis),相对丰度分别为16.68%和4.62%,且硫杆菌属具有提高磷去除能力的功能;而砾石人工湿地中反硝化脱氮菌属主要为类固醇杆菌属(Steroidobacter),相对丰度为4.56%,采用黄铁矿为基质后,人工湿地中的脱氮除磷菌丰度和多样性升高,有利于人工湿地对尾水的脱氮除磷。黄铁矿人工湿地出水存在SO_(4)^(2-)含量偏高的现象,建议工程应用中在人工湿地底部填充黄铁矿以达到出水SO_(4)^(2-)含量降低的目的。

关键词：垂直潜流人工湿地黄铁矿砾石尾水处理效果微生物

GATIS score for predicting the prognosis of rectal neuroendocrine neoplasms:A Chinese multicenter study of 12-year experience

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World Journal of Gastroenterology 2024年第28期30卷 3403-3417页

作者： Xin-Yu Zeng Ming Zhong Guo-Le lin Cheng-Guo li Wei-Zhong Jiang Wei Zhang li-Jian Xia Mao-Jun Di Hong-Xue Wu Xiao-Feng liao Yue-Ming Sun Min-Hao Yu Kai-Xiong Tao Yong li Rui Zhang Peng Zhang Department of Gastrointestinal Surgery Union HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430074Hubei ProvinceChina Department of Gastrointestinal Surgery Renji HospitalSchool of MedicineShanghai Jiao Tong UniversityShanghai 200127China Department of General Surgery Peking Union Medical College HospitalPeking Union Medical CollegeBeijing 100730China Department of Colorectal Surgery Fujian Medical UniversityFuzhou 350401Fujian ProvinceChina Department of Colorectal Surgery Changhai HospitalShanghai 200433China Department of Gastrointestinal Surgery The First Affiliated Hospital of Shandong First Medical UniversityJinan 250118Shandong ProvinceChina Department of Gastrointestinal Surgery Taihe HospitalHubei University of MedicineShiyan 442099Hubei ProvinceChina Department of Gastrointestinal Surgery I Section Renmin Hospital of Wuhan UniversityWuhan 430060Hubei ProvinceChina Department of General Surgery Xiangyang Central HospitalAffiliated Hospital of Hubei University of Arts and ScienceXiangyang 441021Hubei ProvinceChina Department of Colorectal Surgery Jiangsu Province HospitalNanjing Medical UniversityNanjing 210029Jiangsu ProvinceChina Department of Gastrointestinal Surgery School of MedicineShanghai Jiao Tong UniversityShanghai 200127China Department of General Surgery Guangdong Provincial People’s HospitalGuangdong Academy of Medical SciencesGuangzhou 510080Guangdong ProvinceChina Department of Colorectal Surgery Liaoning Cancer HospitalShenyang 110042Liaoning ProvinceChina

BACKGROUND There is currently a shortage of accurate,efficient,and precise predictive instruments for rectal neuroendocrine neoplasms(NENs).AIM To develop a predictive model for individuals with rectal NENs(R-NENs)using data from a large *** Data from patients with primary R-NENs were retrospectively collected from 17 large-scale referral medical centers in *** forest and Cox proportional hazard models were used to identify the risk factors for overall survival and progression-free survival,and two nomograms were *** A total of 1408 patients with R-NENs were *** grade,T stage,tumor size,age,and a prognostic nutritional index were important risk factors for *** GATIS score was calculated based on these five *** overall survival prediction,the respective C-indexes in the training set were 0.915(95%confidence interval:0.866-0.964)for overall survival prediction and 0.908(95%confidence interval:0.872-0.944)for progression-free survival *** to decision curve analysis,net benefit of the GATIS score was higher than that of a single *** time-dependent area under the receiver operating characteristic curve showed that the predictive power of the GATIS score was higher than that of the TNM stage and pathological grade at all time *** The GATIS score had a good predictive effect on the prognosis of patients with R-NENs,with efficacy superior to that of the World Health Organization grade and TNM stage.

关键词： Rectal neuroendocrine neoplasm Nomogram Random forest Prognosis Overall survival Progression-free survival

GABA Enhances Thermotolerance of Seeds Germination by Attenuating the ROS Damage in Arabidopsis

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Phyton-International Journal of Experimental Botany 2020年第3期89卷 619-631页

作者： Qili Zhang Danni He Songbei Ying shiyan Lu Jiali Wei Ping li Shanghai Key Laboratory of Bio-Energy Crops Research Center for Natural ProductsPlant Science CenterSchool of Life SciencesShanghai UniversityShanghai200444China

Seeds germination is strictly controlled by environment factor such as high temperature(HT)through altering the balance between gibberellin acid(GA)and abscisic acid(ABA).Gama-aminobutyric acid(GABA)is a small molecule with four-carbon amino acid,which plays a crucial role during plant physiological process associated with pollination,wounding or abiotic stress,but its role in seeds germination under HT remains *** this study we found that HT induced the overaccumulation of ROS,mainly H_(2)O_(2) and O_(2)^(-),to suppress seeds germination,meanwhile,HT also activated the enzyme activity of GAD for the rapid accumulation of GABA,hinting the regulatory function of GABA in con-trolling seeds germination against HT *** GABA directly attenuated HT-induced ROS accumulation,upregulated GA biosynthesis and downregulated ABA biosynthesis,ultimately enhanced seeds ***,genetic analysis using the gad1/2 mutant defective in GABA biosynthesis,or pop2-5 mutant with high endogenous GABA content supported the potential function of GABA in improving seeds germination tolerance to HT through scavenging ROS *** on these data,we propose that GABA acts as a novel signal to enhance thermotolerance of seeds germination through alleviating the ROS damage to seeds viability.

关键词： GABA thermotolerance ROS damage seeds germination

The BHLH Transcriptional Factor PIF4 Competes with the R2R3-MYB Transcriptional Factor MYB75 to Fine-Tune Seeds Germination under High Glucose Stress

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Phyton-International Journal of Experimental Botany 2021年第5期90卷 1387-1400页

作者： Xiaoli li shiyan Lu Yaru Yang Wenjie Wei Jiali Wei Xiaojun Yuan Ping li Shanghai Key Laboratory of Bio-Energy Crops School of Life SciencesShanghai UniversityShanghai200444China

It is known that the high level of sugar including glucose suppresses seed germination through ABA ***5 is an essential component to mediate ABA-dependent seed germination inhibition,but underlying mechanism needs more *** study demonstrated the PIF4 activated the expression of ABI5 to suppress seed germination in *** we reported that PIF4 also mediated the seed germination inhibition through ABI5 under high concentration of glucose ***,we found that PIF4 interacted with PAP1,the central factor to control anthocyanin *** interaction was confirmed in vitro and in *** and physiological analysis revealed that PAP1 bond the promoter of ABI5 to suppress its expression,thus enhanced seed germination under high concentration of glucose ***,PAP1 competed with PIF4 to antagonize the activation of PIF4 on ABI5 expression,thus promoted seed germination under high glucose *** these,we uncover a novel role for PIF4 and PAP1 in controlling seed germination under high glucose treatment,and reveal their antagonistic mechanism by which coordinates ABI5 expression to control seed germination in response to the glucose signal.

关键词： Seeds germination glucose MYB75 PIF4 ABI5