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Characterization of a Novel Rice Metallothionein Gene Promoter:Its Tissue Specificity and Heavy Metal Responsiveness

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Journal of Integrative Plant Biology 2010年第10期52卷 914-924页

作者： Chun-Juan Dong Yun Wang shi-shi Yu Jin-yuan liu Laboratory of Molecular Biology and MOE Laboratory of Protein Science School of Life Sciences Tsinghua University Beijing 100084China

The rice （Oryza sativa L.） metallothionein gene OsMT-I-4b has previously been identified as a type I MT gene. To elucidate the regulatory mechanism involved in its tissue specificity and abiotic induction, we isolated a 1 730 bp fragment of the OsMT-I-4b promoter region. Histochemical /^-glucuronidase （GUS） staining indicated a precise spacial and temporal expression pattern in transgenic Arabidopsis. Higher GUS activity was detected in the roots and the buds of flower stigmas, and relatively lower GUS staining in the shoots was restricted to the trichomes and hydathodes of leaves. No activity was observed in the stems and seeds. Additionally, in the root of transgenic plants, the promoter activity was highly upregulated by various environmental signals, such as abscisic acid, drought, dark, and heavy metals including Cu2＋, Zn2~, Pb2~ and Al3＋. Slight induction was observed in transgenic seedlings under salinity stress, or when treated with Co2＋ and Cd2＋. Promoter analysis of 5＇-deletions revealed that the region -583/-1 was sufficient to drive strong GUS expression in the roots but not in the shoots. Furthermore, deletion analysis indicated important promoter regions containing different metal- responsive c/s-elements that were responsible for responding to different heavy metals. Collectively, these findings provided important insight into the transcriptional regulation mechanisms of the OsMT-I- 4b promoter, and the results also gave us some implications for the potential application of this promoter in plant genetic engineering.

关键词： PLANT gene expression PLANT genetics METALLOTHIONEIN PROMOTERS (Genetics) HEAVY metals PLANT genetic engineering TRANSGENIC rice ARABIDOPSIS PLANT cells & tissues GENETIC regulation in plants

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A Facile and Environment-friendly Method for Fabrication of Polymer Brush

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Chinese Journal of Polymer Science 2017年第7期35卷 857-865页

作者： Xiao-min Kang Wan-hao Cai Hong-gang Gu shi-yuan liu 崔树勋 Key Laboratory of Advanced Technologies of Materiais Ministry of Education Southwest Jiaotong University Chengdu 610031 China State Key Laboratory of Digital Manufacturing Equipment and Technology Huazhong University of Science and Technology Wuhan 430074 China

A novel environment-friendly system is proposed to fabricate polymer brush, which has the advantages including non-toxic and inexpensive initiator （eosin Y）, visible-light exposure （λ = 515 nm）, water medium and ambient environment. The experimental results from UV-Vis spectroscopy, AFM-based single molecule force spectroscopy （SMFS） and other measurements indicate that a polymer brush with a living nature is fabricated via free radical polymerization. This polymer brush may find applications in coatings, bio-interfaces and so forth.

关键词： Polymer brush Green chemistry Free radical polymerization Living polymerization

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Recovery and separation of Fe and Mn from simulated chlorinated vanadium slag by molten salt electrolysis

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International Journal of Minerals,Metallurgy and Materials 2020年第12期27卷 1678-1686页

作者： shi-yuan liu Yu-lan Zhen Xiao-bo He Li-jun Wang Kuo-chih Chou Collaborative Innovation Center of Steel Technology University of Science and Technology BeijingBeijing 100083China National Engineering Laboratory for Hydrometallurgical Cleaner Production Technology Institute of Process EngineeringChinese Academy of SciencesBeijing 100190China State Key Laboratory of Advanced Metallurgy University of Science and Technology BeijingBeijing 100083China

Tailings from the vanadium extraction process are discarded each year as waste,which contain approximately 30 wt%of *** our previous work,we extracted Fe and Mn from vanadium slag,and Fe and Mn existed in the form of FeCl_(2) and MnCl_(2) after chlorination by NH_(4) Cl to achieve effective and green usage of waste containing Fe and *** this work,square wave voltammetry(SWV)and cyclic voltammetry(CV)were applied to investigate the electrochemical behaviors of Fe^(2+)and Mn^(2+)in Na Cl-KCl melt at 800℃.The reduction processes of Fe^(2+)and Mn^(2+)were found to involve one *** diffusion coefficients of FeCl_(2) and Mn Cl_(2) in molten salt of eutectic mixtures Na Cl-KCl molten salt were *** electrodeposition of Fe and Mn were performed using two electrodes at a constant cell *** Mn/Fe mass ratio of the electrodeposited product in Na Cl-KCl-2.13 wt%FeCl_(2)-1.07 wt%Mn Cl_(2) was 0.0625 at 2.3 *** the electrolysis of NaCl-KCl-2.13 wt%Fe Cl_(2)-1.07 wt%MnCl_(2) melted at 2.3 V,the electrolysis was again started under 3.0 V and the Mn/Fe mass ratio of the electrodeposited product was *** process provides a novel method to effectively separate Fe and Mn from simulated chlorinated vanadium slag.

关键词： vanadium slag molten salt electrolysis separation electrochemical analysis

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Diagnostic value of serum human epididymis protein 4 in esophageal squamous cell carcinoma

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World Journal of Gastrointestinal Oncology 2020年第10期12卷 1167-1176页

作者： shi-yuan liu Muhammad Ahsan Bilal Jian-Hong Zhu Shao-Min Li Department of Thoracic Surgery Second Affiliated Hospital of Xi'an Jiaotong UniversityXi'an 710004Shaanxi ProvinceChina Department of Dermatology and Venereology Second Affiliated Hospital of Xi'an Jiaotong UniversityXi'an 710004Shaanxi ProvinceChina Department of Clinical Laboratory Second Affiliated Hospital of Xi'an Jiaotong UniversityXi'an 710004Shaanxi ProvinceChina

BACKGROUND Numerous studies have demonstrated that human epididymis protein 4(HE4)is overexpressed in various malignant tissues including ovarian,endometrial,lung,breast,pancreatic,and gastric ***,no study has examined the diagnostic impact of HE4 in patient with esophageal squamous cell carcinoma(ESCC)until *** To analyze the value of four serum tumor markers for the diagnosis of ESCC,and examine the associations of serum levels of HE4 with ESCC patients’clinicopathological *** The case group consisted of 80 ESCC patients,which were compared to a control group of 56 patients with benign esophageal *** levels of HE4,carcinoma embryonic antigen(CEA),alpha fetal protein,and carbohydrate antigen 19-9(CA19-9)were detected by *** associations of serum HE4 levels with ESCC patients’clinicopathological characteristics such as gender,tumor location,and pathological stage were also examined after *** The result of ELISA showed that serum HE4 level was significantly higher in the patients with ESCC than in the controls,and the staining intensity was inversely correlated with the pathological T and N *** HE4 levels had a sensitivity of 66.2%and specificity of 78.6%when the cutoff value was set at 3.9 ng/***,the combined HE4 and CA19-9 increased the sensitivity to 83.33%,and interestingly,the combination of HE4 with CEA led to the most powerful sensitivity of 87.5%.Furthermore,A positive correlation was observed between HE4 serum levels and pathological T and N stages(P=0.0002 and 0.0017,respectively),but there was no correlation between HE4 serum levels and ESCC patient gender(P=0.4395)or tumor location(P=0.6777).CONCLUSION The results of this study suggest that detection of serum HE4 levels may be useful in auxiliary diagnosis and evaluation of the progression of ESCC.

关键词： Human epididymis protein 4 Esophageal squamous cell carcinoma Diagnostic value Histological stage Clinicopathological characteristics Clinical progression

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PIK3CA gene mutations in Northwest Chinese esophageal squamous cell carcinoma

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World Journal of Gastroenterology 2017年第14期23卷 2585-2591页

作者： shi-yuan liu Wei Chen Ehtesham Annait Chughtai Zhe Qiao Jian-Tao Jiang Shao-Min Li Wei Zhang Jin Zhang Department of Thoracic Surgery Second Affiliated Hospital of Xi'an Jiaotong University Department of Medical Biochemistry and Molecular Biology School of Basic Medical Sciences Lanzhou University Xi'an Jiaotong University School of Medicine

AIM To evaluate PIK3 CA gene mutational status in Northwest Chinese esophageal squamous cell carcinoma(ESCC) patients, and examine the associations of PIK3 CA gene mutations with clinicopathological characteristics and clinical *** A total of 210 patients with ESCC who underwent curative resection were enrolled in this study. Pyrosequencing was applied to investigate mutations in exons 9 and 20 of PIK3 CA gene in 210 Northwest Chinese ESCCs. The associations of PIK3 CA gene mutations with clinicopathological characteristics and clinical outcome were *** PIK3 CA gene mutations in exon 9 were detected in 48 cases(22.9%) of a non-biased database of 210 curatively resected Northwest Chinese ESCCs. PIK3 CA gene mutations were not associated with sex, tobacco use, alcohol use, tumor location, stage, or local recurrence. When compared with wild-type PIK3 CA gene cases, patients with PIK3 CA gene mutations in exons 9 experienced significantly better disease-free survival and overall survival *** The results of this study suggest that PIK3 CA gene mutations could act as a prognostic biomarker in Northwest Chinese ESCC patients.

关键词： PIK3CA gene mutations Esophageal squamous cell carcinoma Northwest Chinese Prognostic significance

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Changes in macrophage infiltration and podocyte injury in lupus nephritis patients with repeated renal biopsy: Report of three cases

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World Journal of Clinical Cases 2024年第1期12卷 188-195页

作者： shi-yuan liu Hao Chen Li-Jia He Chun-Kai Huang Pu Wang Zhang-Ru Rui Jue Wu Yang yuan Yue Zhang Wen-Ju Wang Xiao-Dan Wang Central Laboratory Key Laboratory of Tumor Immunological Prevention and Treatment of Yunnan ProvinceYan'an Hospital Affiliated to Kunming Medical UniversityKunming 650051Yunnan ProvinceChina Department of Nephropathy Yan'an Hospital Affiliated to Kunming Medical UniversityKunming 650051Yunnan ProvinceChina

BACKGROUND In this study,we retrospectively analysed macrophage infiltration and podocyte injury in three patients with diffuse proliferative lupus nephritis(LN)who un-derwent repeated renal *** SUMMARY Clinical data of three diffuse proliferative LN patients with different pathological characteristics(case 1 was LN IV-G(A),case 2 was LN IV-G(A)+V,and case 3 was LN IV-G(A)+thrombotic microangiopathy)were *** patients underwent repeated renal biopsies 6 mo later,and renal biopsy specimens were *** infiltration was assessed by CD68 expression detected by immunohistochemical staining,and an immunofluorescence assay was used to detect podocin expression to assess podocyte *** treatment,Case 1 changed to LN III-(A),Case 2 remained as type V LN lesions,and Case 3,which changed to LN IV-S(A),had the worst *** observed reduced macro-phage infiltration after ***,two of the patients with active lesions after treatment still showed macrophage infiltration in the renal *** treatment,the three patients showed discontinuous expression of ***,the integrity of podocin was restored after treatment in Case *** It may be possible to reverse podocyte damage and decrease the infiltrating ma-crophages in LN patients through effective treatment.

关键词： Lupus nephritis Macrophage Podocyte Repeat renal biopsy Thrombotic microangiopathy Case report

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基于二硫化碳解吸前处理技术GC-FID测定工作场所空气中烷烃

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现代化工 2017年第7期37卷 203-205页

作者：袁世柳李世霞江苏华创检测技术服务有限公司江苏南通226000 中国石油大学(华东) 山东青岛266580

为建立工作场所空气中正戊烷、正己烷、正庚烷3种烷烃有毒物质的溶剂解吸-气相色谱检测法,采用二硫化碳解吸脱附作为前处理手段,利用TG-5MS毛细管柱对目标物质进行分离,以氢火焰离子化检测器进行检测,保留时间定性,峰面积响应值定量,外... 详细信息

为建立工作场所空气中正戊烷、正己烷、正庚烷3种烷烃有毒物质的溶剂解吸-气相色谱检测法,采用二硫化碳解吸脱附作为前处理手段,利用TG-5MS毛细管柱对目标物质进行分离,以氢火焰离子化检测器进行检测,保留时间定性,峰面积响应值定量,外标法计算。结果表明:该方法能够很好地分离并检测定量出正戊烷、正己烷、正庚烷各自含量,各自标准曲线相关系数均达到0.999 0,精密度在1.83%~5.79%范围内,实际样品加标回收率在89.6%~97.8%之间,该方法检出限:正戊烷为0.008 6μg/m L,正己烷为0.200 0μg/m L,正庚烷为0.056 6μg/m L。

关键词：烷烃二硫化碳解吸气相色谱法

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泵浦激光功率和气室温度对SERF陀螺仪的影响（英文）

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中国惯性技术学报 2017年第6期25卷 810-814,820页

作者：石猛刘院省王学锋王巍北京航天控制仪器研究所北京100854

SERF陀螺仪工作在无自旋交换弛豫(SERF)状态下,电子自旋和核子自旋强烈耦合。通过研究电子自旋与核子自旋的耦合方程,分析了泵浦激光的功率和气室温度对SERF陀螺仪磁场补偿的影响,并搭建了SERF陀螺仪实验平台系统进行验证。研究结果表明... 详细信息

SERF陀螺仪工作在无自旋交换弛豫(SERF)状态下,电子自旋和核子自旋强烈耦合。通过研究电子自旋与核子自旋的耦合方程,分析了泵浦激光的功率和气室温度对SERF陀螺仪磁场补偿的影响,并搭建了SERF陀螺仪实验平台系统进行验证。研究结果表明:原子核自旋补偿磁场正比于泵浦激光功率,泵浦激光功率在9.7 m W至105.6 m W的变化范围中,自旋补偿磁场的变化达到8.9 n T;气室温度在107.5℃至149.3℃变化范围中,自旋补偿磁场变化为4.5 n T,无明显的相关性,并且相对于气室温度的变化,补偿磁场对泵浦激光功率变化更加敏感。

关键词： SERF陀螺仪原子自旋陀螺仪无自旋交换弛豫自旋耦合

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Effect of b value on monitoring therapeutic response by diffusion-weighted imaging

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World Journal of Gastroenterology 2008年第38期14卷 5893-5899页

作者： Zhao-Xia Jiang Wei-Jun Peng Wen-Tao Li Feng Tang shi-yuan liu Xu-Dong Qu Jian-Hua Wang Hong-Feng Lu Department of Radiology Affiliated Cancer Hospital of Fudan University Shanghai 200032 China Department of Oncology Shanghai Medical College Fudan University Shanghai 200032 China Department of Radiology Affiliated Changzheng Hospital of the Second Military Medical University Shanghai 200032 China Department of Radiology Affiliated Zhongshan Hospital of Fudan University Shanghai 200032 China Department of Pathology Affiliated Cancer Hospital of Fudan University Shanghai 200032 China

AIM： To explore the diffusion gradient b-factor that optimizes both apparent diffusion coefficient （ADC） measurement and contrast-to-noise （CNR） for assessing tumor response to transarterial chemoembolization （TACE） in a rabbit model. METHODS： Twelve New Zealand white rabbits bearing VX2 tumors in the liver were treated with TACE. Diffusion-weighted imaging （DWI） with various b values was performed using the same protocol before and 3 d after treatment with TACE. ADC values and CNR of each tumor pre- and post-treatment with different b factors were analyzed. Correlation between ADC values and extent of necrosis in histological specimens was analyzed by a Pearson＇s correlation ***： The quality of diffusion-weighted images diminished as the b value increased. A substantial decrease in the mean lesion-to-liver CNR was observed on both pre- and post-treatment DW images, the largest difference in CNR pre- and post-treatment was manifested at a b value of 1000 s/mm^2 （P = 0.036 ）. The effect of therapy on diffusion early after treatment was shown by a significant increase in ADCs （P = 0.007）, especially with large b factors （≥ 600 s/mm^2）. The mean percentage of necrotic cells present within the tumor was 76.3%-97.5%. A significant positive correlation was found between ADC values and the extent of necrosis with all b values except for b200, a higher relative coefficient between ADC values and percentage of necrosis was found on DWI with bl000 and b2000 （P = 0.002 and 0.006, respectively）. CONCLUSION： An increasing b value of up to 600 s/mm^2 would increase ADC contrast pre- and post-treatment, but decrease image quality. Taking into account both CNR and ADC measurement, diffusion-weighted imaging obtained with a b value of 1000 s/mm^2 is recommended for monitoring early hepatic tumor response to TACE.

关键词： Hepatic carcinoma Diffusion-weighted MR Treatment response Apparent diffusion coefficient

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磁共振成像在肺癌诊断和鉴别诊断中的应用和研究现状

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当代医学 2009年第8期15卷 124-131页

作者：范丽刘士远第二军医大学长征医院影像科上海200003

肺癌的影像诊断目前主要还是依靠X线和CT以及PET—CT，MR／检查具有软组织对比分辨率高、多参数成像、无需重建的任意断面成像、无骨伪影、无放射线损伤等优点，使其在胸部疾病尤其是肺癌的诊断中具有一定的补充作用。本文从胸部检查方... 详细信息

肺癌的影像诊断目前主要还是依靠X线和CT以及PET—CT，MR／检查具有软组织对比分辨率高、多参数成像、无需重建的任意断面成像、无骨伪影、无放射线损伤等优点，使其在胸部疾病尤其是肺癌的诊断中具有一定的补充作用。本文从胸部检查方法、肺癌TNM分期、DCE—MRI、DWI、MRS和肺功能成像几个方面评价MRI在肺癌诊断和鉴别诊断中的作用。

关键词：肺癌诊断鉴别诊断磁共振成像胸部疾病放射线损伤 TNM分期肺功能成像 MRI

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