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Proteomic Alterations of Antarctic Ice Microalga Chlamydomonas *** Low-Temperature Stress

Journal of Integrative Plant Biology 2006年第8期48卷 965-970页

作者： Guang-Feng Kan Jin-Lai Miao Cui-Juan shi Guang-you li School of the Ocean Harbin Institute of Technology in Weihai Weihai 264209 China Key Laboratory of Marine Bio-active Substances State Oceanic Administration Qingdao 266061 China

Antarctic ice microalga can survive and thrive in cold channels or pores in the Antarctic ice layer. In order to understand the adaptive mechanisms to low temperature, in the present study we compared two-dimensional polyacrylamide gel electrophoresis （2-DE） profiles of normal and low temperature-stressed Antarctic ice microalga Chlamydomonas sp. cells. In addition, new protein spots induced by low temperature were identified with peptide mass fingerprinting based on matrix-assisted laser desorption/ionization time-of-flight mass spectrometry （MALDI-TOF-MS） and database searching. Well-resolved and reproducible 2-DE patterns of both normal and low temperature-stressed cells were acquired. A total of 626 spots was detected in control cells and 652 spots were detected in the corresponding low temperature-stressed cells. A total of 598 spots was matched between normal and stressed cells. Two newly synthesized proteins （a and b） in low temperature-stressed cells were characterized. Protein spot A （53 kDa, pl 6.0） was similar to isopropylmalate/homocitrate/citramalate synthases, which act in the transport and metabolism of amino acids. Protein spot b （25 kDa, pl 8.0） was related to glutathione S-transferase, which functions as a scavenger of active oxygen, free radicals, and noxious metabolites. The present study is valuable for the application of ice microalgae, establishing an ice microalga Chlamydomonas sp. proteome database, and screening molecular biomarkers for further studies.

关键词： Antarctic ice microalga Chlamydomonas sp two-dimensional polyacrylamide gel electrophoresis differentialexpression protein low temperature stress matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS).

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肾结石(草酸钙)形成的影响因素和超声的诊断价值

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国外医学（医学地理分册） 2005年第2期26卷 94-96,F003页

作者：韩生兰刘军史有利刘进富昭马家莲子长县医院陕西子长717300 西安交通大学第一医院影像中心陕西西安710061 宝鸡石油医院急诊科陕西宝鸡721000 西安交通大学外语部陕西西安710061 西安交通大学第一医院超声室陕西西安710061

目的探讨肾结石形成的影响因素和超声检查的诊断价值。方法使用凸阵探头,对158例肾结石患者进行超声检查,并与CT、X线平片和静脉肾盂造影进行对比分析。结果本组病例均有不同程度的腰部不适、肾绞痛和血尿等临床表现,超声检查表现为肾... 详细信息

目的探讨肾结石形成的影响因素和超声检查的诊断价值。方法使用凸阵探头,对158例肾结石患者进行超声检查,并与CT、X线平片和静脉肾盂造影进行对比分析。结果本组病例均有不同程度的腰部不适、肾绞痛和血尿等临床表现,超声检查表现为肾脏内强回声光团及其后方伴随声影的结石病灶显示,可引起肾周水肿,且肾周水肿的出现与结石发生的部位有关。结论肾草酸钙结石的形成可能与尿草酸排泄过多和草酸的代谢异常有关。伴随肾结石强回声后方的声影为肾结石的另一超声学表现,超声检查能清晰地显示肾内液区,对肾结石具有确诊价值。

关键词：诊断价值超声检查静脉肾盂造影肾周水肿肾结石形成草酸钙结石凸阵探头结石患者对比分析 X线平片不同程度临床表现结石病灶代谢异常强回声肾绞痛检查表尿草酸超声学显示声影后方

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Orientation and Structure of Ionic liquid Cation at Air/[bmim][BF4] Aqueous Solution Interface

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Chinese Journal of Chemical Physics 2013年第5期26卷 569-575,I0004页

作者： Gang-hua Deng Xia li you-qi Guo shi-lin liu Zhou Lu Yuan Guo Hefei National Laboratory for Physical Sciences at the Microscale University of Science and Tech-nology of China Hefei 230026 China Beifing National Laboratory for Molecular Sciences State Key Laboratory of Molecular ReactionDynamics Institute of Chemistry Chinese Academy of Sciences Belting 100190 China Nanjing University of Aeronautics and Astronautics NanjinE 211205 China

The watermiscible room temperature ionic liquid 1butyl3methylimidazolium tetrafluorob orate （[bmim] [BF4]） is a model system for studying the interactions between ionic liquid and water molecules. In this work the orientational structure of the low concentrated aqueous solution of [bmim] [BF4] at the air/liquid interface was investigated by sum frequency gener ation vibrational spectroscopy. It has been found that at very low concentrations, the butyl chain exhibited a significant gauche defect, indicating a disordered conformation; and the cation ring oriented with a fairly small tilting angle at the surface. When the concentration increased, the cation ring tended to lie flat at the surface, and the gauche defects of the butyl chain decreased due to the intermolecular chainchain interactions and the consequent more ordered interfacial molecular arrangement. Additionally, the antisymmetric stretching mode in the PPP and SPS spectra exhibited a peak shift, showing that there exists more than one kind of orientation or chemical environment for the butyl CH3 group. These results may shed new light on understanding the surface behavior of watermiscible ionic liquids as well as the imidazolium based surfactants.

关键词： Sum frequency generation vibratinal spectroscopy Ionic liquid Aqueous solu-tion Interface orientation

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输尿管钙石的超声影像学研究

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国外医学（医学地理分册） 2005年第1期26卷 47-48,F003-F004页

作者：韩生兰刘军史有利刘进富昭马家莲子长县医院陕西子长717300 西安交通大学第一医院影像中心陕西西安710061 宝鸡石油医院急诊科陕西宝鸡721000 西安交通大学外语部西安交通大学第一医院超声室陕西西安710061

目的　探讨输尿管钙石形成的机制和超声检查的诊断价值。方法　使用凸阵探头 ,对 1 36例输尿管钙石患者进行超声检查 ,并与 CT、X线平片和静脉肾盂造影进行对比分析。结果　本组病例均有不同程度的腰部不适、肾绞痛和血尿等临床表现 ,... 详细信息

目的　探讨输尿管钙石形成的机制和超声检查的诊断价值。方法　使用凸阵探头 ,对 1 36例输尿管钙石患者进行超声检查 ,并与 CT、X线平片和静脉肾盂造影进行对比分析。结果　本组病例均有不同程度的腰部不适、肾绞痛和血尿等临床表现 ,超声检查表现为输尿管内径大于 4mm,在扩大的输尿管管腔内见有强回声的钙石病灶显示 ,可引起肾周水肿且输尿管钙石引起的肾周水肿以肾的上极和下极多见。结论　在一些地区输尿管钙石的形成可能与水的硬度有关。超声检查能清楚地显示输尿管内液区及钙石病灶并具有确诊价值 ,对临床有明显症状且高度怀疑输尿管钙石的患者应首选超声检查。

关键词：输尿管超声检查超声影像学病灶患者腔内强回声形成高度探头

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Copper-catalyzed Meerwein carboarylation of alkenes with anilines to form 3-benzyl-3-alkyloxindole

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Science China Chemistry 2015年第4期58卷 684-688页

作者： shi Tang Dong Zhou youlin Deng Zhihao li you Yang Jianguang He Yingchun Wang College of Chemistry and Chemical Engineering Central South University College of Chemistry and Chemical Engineering Jishou University Key Laboratory for the Chemistry and Molecular Engineering of Medicinal Resources(Ministry of Education of China) Guangxi Normal University

A simple and direct route for double C–C bond formation by copper-catalyzed Meerwein carboarylation process has been developed. In the presence of Cu I(5 mol%), tert-butyl nitrite and anilines, a wide variety of N-arylacrylamides underwent tandem Meerwein arylation/C–H cyclization to produce pharmaceutically important 3-benzyl-3-alkyloxindole in moderate to good yield.

关键词： radical reaction C-H cyclization Meerwein arylation oxindole copper-catalyzed

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Major physiological adjustments in freezing-tolerant grey tiger longicorn beetle(Xylotrechus rusticus) during overwintering period

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Journal of Forestry Research 2014年第3期25卷 653-659页

作者： Jue-wen li Juan shi Yu Xue Hong-bo Mao you-qing Luo Beijing Key Laboratory of Forest Pest Control Beijing Forestry University College of Forestry Northeast Forestry University Forestry Administration of Acheng District

1Xylotrechus rusticus （linnaeus） is one of the most destruc-tive woodborers in northeast China;it damages poplar and is listed as a domestic forestry quarantine pest. Overwintering larvae were collected during October 2012 and March 2013 in Harbin, China, to quantify indi-cators related to the insect’s overwintering strategy and the major cryo-protectants. The supercooling points （SCPs）, which ranged from-14.7＆#176;C to -2.9＆#176;C, were higher than the lethal temperatures of LT50 （-33.64＆#176;C） and LT99 （-40.17＆#176;C） after 24 h exposure. , also the minimum mean daily temperature （-24.5＆#176;C） and mean monthly temperature （-18.0＆#176;C） at the sampling site in January during 2008-2012. Thus, X. rusticus is a typical freezing-tolerant insect. Glycerol serves as a major cryoprotectant for overwintering larvae , because it was the only polyol accumulated during the winter and it also had a significant negative correlation with the SCP （p=0.033, R=0.907）. The glycogen and lipid are major sources of ener-gy and their levels decreased substantially in the middle of overwintering, when glycogen had a significant correlation with the SCP （p= 0.006, R=0.971） whereas the lipid contents did not. Moreover, inter-conversions between glycerol and glycogen, as well as mannose and glycogen, were suggested by their negative correlations. The water content did not change obviously during the winter and was not correlated with the SCP. The free amino acids in the hemolymph and the total protein contents of the bodies of larvae changed significantly during winter, although both had no correlations with the SCP.

关键词： cold hardiness cryoprotectant freezing tolerant physiological variation Xylotrechus rusticus.

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Fabrication and efficient electromagnetic waves attenuation of three-dimensional porous reduced graphene oxide/boron nitride/silicon carbide hierarchical structures

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Journal of Materials Science & Technology 2023年第24期155卷 192-201页

作者： Qiuqi Zhang Xiao you li Tian Mengmeng Wang Xiangyu Zhang Ying shi Jinshan Yang Shaoming Dong School of Materials Science and Engineering Shanghai University Shanghai 200444 China State Key Laboratory of High Performance Ceramics & Superfine Microstructure Shanghai Institute of Ceramics Chinese Academy of Sciences Shanghai 200050 China Structural Ceramics and Composites Engineering Research Center Shanghai Institute of Ceramics Chinese Academy of Sciences Shanghai 200050 China University of Chinese Academy of Sciences Beijing 100049 China Center of Materials Science and Optoelectronics Engineering University of Chinese Academy of Sciences Beijing 100049 China

In this work,hierarchical hybrid composites consisting of porous three-dimensional reduced graphene oxide(3D-rGO)skeleton and lamellar boron nitride(BN)/silicon carbide(SiC)coatings are prepared by chemical vapor infiltration(CVI)*** graphene framework prepared by 3D printing and frozen self-assembly exhibits a lightweight structure and a perforated conductive network,which extends the transmission path of incident *** introduced ceramic coatings can effectively tune the impedance matching degree and supply a lossy phase,and the hierarchical structure of the composites enhances the multiple scattering of the incident *** expected,the 3D-rGO/BN/SiC composites possess an excellent absorbing performance with a minimum reflection loss value of–37.8 dB,and the widest effective absorbing bandwidth(RLline for rational design and fabrication of high-performance electromagnetic waves absorbing materials in practical applications.

关键词： Porous network structure Hierarchical structure Electromagnetic wave absorbing High attenuation ability Effective absorbing bandwidth

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强碱体系下制备铈锆复合氧化物

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稀有金属与硬质合金 2003年第2期31卷 1-4页

作者：郝仕油李进周雪珍李永锈浙江师范大学生命与环境科学院浙江金华321004 南昌大学稀土工程中心江西南昌330047

在强碱作用下制得了铈锆超细氧化物,借助于X射线衍射、热重、激光粒度仪、比表面仪对该粉末的性能进行了研究。研究表明:随着锆含量的增加,Zr-Ce-O氧化物颗粒有增大的趋势;无水乙醇是一种较好的洗涤剂;作为反应原料,硝酸盐较氯化盐好;... 详细信息

在强碱作用下制得了铈锆超细氧化物,借助于X射线衍射、热重、激光粒度仪、比表面仪对该粉末的性能进行了研究。研究表明:随着锆含量的增加,Zr-Ce-O氧化物颗粒有增大的趋势;无水乙醇是一种较好的洗涤剂;作为反应原料,硝酸盐较氯化盐好;在强碱下,干磨比湿磨好。

关键词：强碱超细Zr—Ce—O粉末性能

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In situ dynamics response mechanism of the tunable length-diameter ratio nanochains for excellent microwave absorber

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Nano Research 2020年第1期13卷 72-78页

作者： Wenbin you Ke Pei liting Yang Xiao li Xiaofeng shi Xuefeng Yu Huiqiao Guo Renchao Che Laboratory of Advanced Materials Department of Materials Science and CollaborativeInnovation Center of Chemistry for Energy Materials(iChem)Fudan University220 Handan RoadShanghai200433China

Faster response benefits the high-performance of magnetic material in various live ***,enhancing response speed toward the applied field via engineering advantages in structures is highly *** this paper,the precise synthesis of Co nanochain with the tunable length-diameter ratio is realized via a magnetic-field-guided assembly *** Co nanochain exhibits enhanced microwave absorption performance(near to-60 dB,layer thickness 2.2 mm)and broader effective absorption bandwidth(over 2/3 of total S,C,X,Ku bands).Furthermore,the simulated dynamic magnetic response reveals that the domain motion in 1D chain is faster than that in 0D nanoparticle,which is the determining factor of magnetic loss ***,based on the controllable magnetic field experiment via in situ transmission electron microscopy,the association between magnetic response and microstructure is first present at the *** real and imaginary parts of relative complex permeability are determined by the domain migration confined inside Co nanochain and the magnetic flux field surrounded outside Co nanochain,***,these findings can be extended to the novel design of microwave absorbers and promising candidates of magnetic carriers based on 1D structure.

关键词： microwave absorption in situ transmission electron microscopy(TEM) magnetic materials cobalt nanochain dynamic response

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Proposal of a new T-stage classification system for ampullary carcinoma based on Surveillance,Epidemiology and End Result(SEER)database

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Hepatobiliary & Pancreatic Diseases International 2021年第6期20卷 568-573页

作者： shi-Jie Wang Yi-Fei li Shan liao you-Zhu Wei Yan-Ming Zhou Department of Hepatobiliary and Pancreatovascular Surgery First Affiliated Hospital of Xiamen UniversityXiamen 361003China

Background:Tumor size is still considered a useful prognostic factor in currently available tumor-nodemetastasis(TNM)classification staging systems for most solid tumors,but the significance of tumor size on the prognosis of ampullary carcinoma remains *** aim of the current study was to propose a new T-stage classification system for ampullary carcinoma to address the impact of tumor size on the prognostic ***:Using the Surveillance,Epidemiology,and End Results(SEER)database,we identified 1080 patients with ampullary carcinoma who underwent radical surgical resection between 2004 and *** on the results obtained from analysis of various clinicopathologic factors,a new T-stage classification system was ***:Among the 1080 patients,618 were men and 462 were women,with a median tumor size of 2.3(range 0.1–12)*** the 7 th edition of the American Joint Committee on Cancer(AJCC)staging manual,we noticed significant differences in overall survival(OS)between T2 vs.T3 tumors(P<0.001)and T3 vs.T4 tumors(P=0.002),but failed to observe significant differences between T1 vs.T2 tumors(P=0.498)in our pair-wise *** the newly developed T-stage classification system,we were able to differentiate significant differences in OS between T1 vs.T2 tumors(P=0.032),T2 vs.T3 tumors(P<0.001)and T3 vs.T4 tumor(P=0.003)in all pair-wise *** c-index of the new staging system was 0.653(95%CI:0.629–0.677),showing a better discriminatory power than the 0.636 of the 7 th AJCC staging system(95%CI:0.612–0.660).Conclusions:The new T-stage classification system described herein can better differentiate prognostic outcomes after radical resection in patients with ampullary carcinoma by incorporating tumor size and depth of tumor infiltration.

关键词： Ampullary carcinoma Cancer staging SEER

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