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3D Printing of Tough Hydrogel Scaffolds with Functional Surface Structures for Tissue Regeneration

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Nano-Micro Letters 2025年第2期17卷 18-45页

作者： Ke Yao Gaoying Hong Ximin Yuan Weicheng Kong Pengcheng Xia Yuanrong li Yuewei Chen Nian liu Jing He Jue shi Zihe Hu Yanyan Zhou Zhijian Xie Yong He State Key Laboratory of Fluid Power and Mechatronic Systems School of Mechanical EngineeringZhejiang UniversityHangzhou 310027People’s Republic of China liangzhu Laboratory Zhejiang UniversityHangzhou 310027People’s Republic of China Key Laboratory of 3D Printing Process and Equipment of Zhejiang Province College of Mechanical EngineeringZhejiang UniversityHangzhou 310027People’s Republic of China Stomatology Hospital School of StomatologyZhejiang University School of MedicineHangzhou 310027People’s Republic of China State Key Laboratory of Advanced Welding and Joining Harbin Institute of TechnologyHarbin 150001People’s Republic of China Institute of Digital Medicine Nanjing First HospitalNanjing Medical UniversityNanjing 210006People’s Republic of China

Hydrogel scaffolds have numerous potential applications in the tissue engineering ***,tough hydrogel scaffolds implanted in vivo are seldom reported because it is difficult to balance biocompatibility and high mechanical *** by Chinese ramen,we propose a universal fabricating method(printing-P,training-T,cross-linking-C,PTC&PCT)for tough hydrogel scaffolds to fill this ***,3D printing fabricates a hydrogel scaffold with desired structures(P).Then,the scaffold could have extraordinarily high mechanical properties and functional surface structure by cycle mechanical training with salting-out assistance(T).Finally,the training results are fixed by photo-cross-linking processing(C).The tough gelatin hydrogel scaffolds exhibit excellent tensile strength of 6.66 MPa(622-fold untreated)and have excellent ***,this scaffold possesses functional surface structures from nanometer to micron to millimeter,which can efficiently induce directional cell ***,this strategy can produce bionic human tissue with mechanical properties of 10 kPa-10 MPa by changing the type of salt,and many hydrogels,such as gelatin and silk,could be improved with PTC or PCT *** experiments show that this scaffold can effectively promote the new generation of muscle fibers,blood vessels,and nerves within 4 weeks,prompting the rapid regeneration of large-volume muscle loss injuries.

关键词： 3D printing Tough hydrogel scaffold Functional surface structure Tissue regeneration Biomaterials

降钙素原在环磷酰胺治疗系统性红斑狼疮感染与活动期的鉴别价值及临床意义

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中国合理用药探索 2019年第2期16卷 18-20,23页

作者：韩丹史丽璞李陶冉郇稳郑州人民医院风湿免疫科河南郑州450003

目的:探讨降钙素原(PCT)在环磷酰胺治疗系统性红斑狼疮(SLE)感染与活动期的鉴别价值及临床意义。方法:选取2016年2月—2017年2月我院收治的SLE感染患者40例与SLE活动期患者40例,分为SLE感染组及SLE活动期组,两组均应用环磷酰胺治疗,比... 详细信息

目的:探讨降钙素原(PCT)在环磷酰胺治疗系统性红斑狼疮(SLE)感染与活动期的鉴别价值及临床意义。方法:选取2016年2月—2017年2月我院收治的SLE感染患者40例与SLE活动期患者40例,分为SLE感染组及SLE活动期组,两组均应用环磷酰胺治疗,比较两组治疗前后PCT水平以及阳性表达情况。结果:SLE感染组PCT阳性表达情况显著高于SLE活动期组,差异有统计学意义(P <0.05);治疗后SLE感染组PCT水平高于SLE活动期组,差异有统计学意义(P <0.05)。结论:PCT水平能够早期鉴别SLE感染及活动期,为环磷酰胺的合理使用提供可靠依据,对于改善SLE患者预后具有重要价值。

关键词：系统性红斑狼疮降钙素原环磷酰胺鉴别价值临床意义

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基于BP神经网络的稻瘟病预测预报研究

西南农业学报 2017年第7期30卷 1546-1553页

作者：刘庭洋李烨浦仕磊李志宇李文静吴奇王云月云南农业大学植物保护学院/教育部生物多样性与病害防控重点实验室云南昆明650201

【目的】稻瘟病是水稻主要病害之一,严重制约水稻高产稳产。近年来随着品种布局、耕作制度改变及气候变化的影响,其流行程度年度间波动很大。目前,稻瘟病在云南省各水稻产区呈现中等偏重发生的趋势,预测预报作为指导防治的先行者,具有... 详细信息

【目的】稻瘟病是水稻主要病害之一,严重制约水稻高产稳产。近年来随着品种布局、耕作制度改变及气候变化的影响,其流行程度年度间波动很大。目前,稻瘟病在云南省各水稻产区呈现中等偏重发生的趋势,预测预报作为指导防治的先行者,具有重要作用。【方法】为了及时有效的做好稻瘟病防范工作,本研究采用稻瘟病发生相关气象因子及田间穗瘟病情指数,利用BP神经网络技术,选取德宏州芒市为试验点开展稻瘟病预测预报研究。【结果】从气象因子与预测对象的相关性来看,筛选出来的各气象因子与病情指数之间都存在较强的相关性,其理想输出和实际输出值都比较接近,误差曲线也比较吻合,预测准确度能满足实际需求。【结论】由此可见,BP神经网络建立的稻瘟病中期预测模型更具有优势。不需要事先进行数学公式表达,具有更高的预测准确度,选择试验点5-9月的气象数据以及田间稻瘟病病情指数建立的预测预报模型,预测结果更为客观和可靠,能及时做好稻瘟病的防控工作。

关键词：稻瘟病 BP神经网络预测预报

Single-cell omics: experimental workflow, data analyses and applications

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Science China life Sciences 2025年第1期68卷 5-102页

作者： Fengying Sun Haoyan li Dongqing Sun Shaliu Fu Lei Gu Xin Shao Qinqin Wang Xin Dong Bin Duan Feiyang Xing Jun Wu Minmin Xiao Fangqing Zhao Jing-Dong J.Han Qi liu Xiaohui Fan Chen li Chenfei Wang Tieliu shi Department of Clinical Laboratory the Affiliated Wuhu Hospital of East China Normal University (The Second People's Hospital of Wuhu City) Pharmaceutical Informatics Institute College of Pharmaceutical SciencesZhejiang University Key Laboratory of Spine and Spinal Cord Injury Repair and Regeneration (Tongji University) Ministry of EducationOrthopaedic DepartmentTongji HospitalBioinformatics DepartmentSchool of Life Sciences and TechnologyTongji University Frontier Science Center for Stem Cells School of Life Sciences and TechnologyTongji University Translational Medical Center for Stem Cell Therapy and Institute for Regenerative Medicine Shanghai East HospitalBioinformatics DepartmentSchool of Life Sciences and TechnologyTongji University Research Institute of Intelligent Computing Zhejiang Lab Shanghai Research Institute for Intelligent Autonomous Systems Center for Single-cell Omics School of Public HealthShanghai Jiao Tong University School of Medicine National Key Laboratory of Chinese Medicine Modernization Innovation Center of Yangtze River DeltaZhejiang University Center for Bioinformatics and Computational Biology Shanghai Key Laboratory of Regulatory Biologythe Institute of Biomedical Sciences and School of Life SciencesEast China Normal University Beijing Institutes of life Science Chinese Academy of Sciences Peking-Tsinghua Center for life Sciences Academy for Advanced Interdisciplinary StudiesCenter for Quantitative Biology (CQB)Peking University Zhejiang Key Laboratory of Precision Diagnosis and Therapy for Major Gynecological Diseases Women's HospitalZhejiang University School of Medicine Key Laboratory of Advanced Theory and Application in Statistics and Data Science-MOE School of StatisticsEast China Normal University

Cells are the fundamental units of biological systems and exhibit unique development trajectories and molecular features. Our exploration of how the genomes orchestrate the formation and maintenance of each cell, and control the cellular phenotypes of various organismsis, is both captivating and intricate. Since the inception of the first single-cell RNA technology, technologies related to single-cell sequencing have experienced rapid advancements in recent years. These technologies have expanded horizontally to include single-cell genome, epigenome,proteome, and metabolome, while vertically, they have progressed to integrate multiple omics data and incorporate additional information such as spatial sc RNA-seq and CRISPR screening. Single-cell omics represent a groundbreaking advancement in the biomedical field,offering profound insights into the understanding of complex diseases, including cancers. Here, we comprehensively summarize recent advances in single-cell omics technologies, with a specific focus on the methodology section. This overview aims to guide researchers in selecting appropriate methods for single-cell sequencing and related data analysis.

关键词： single-cell sequencing genome epigenome proteomics metabolomics multimodal spatial transcriptomics CRISPR screening

Metabolic reprogramming: a new option for the treatment of spinal cord injury

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Neural Regeneration Research 2025年第4期20卷 1042-1057页

作者： Jiangjie Chen Jinyang Chen Chao Yu Kaishun Xia Biao Yang Ronghao Wang Yi li Kesi shi Yuang Zhang Haibin Xu Xuesong Zhang Jingkai Wang Qixin Chen Chengzhen liang Department of Orthopedics The Second Affiliated Hospital of Zhejiang University School of MedicineHangzhouZhejiang ProvinceChina Orthopedics Research Institute of Zhejiang University Zhejiang UniversityHangzhouZhejiang ProvinceChina Key Laboratory of Motor System Disease Research and Precision Therapy of Zhejiang Province HangzhouZhejiang ProvinceChina Clinical Research Center of Motor System Disease of Zhejiang Province HangzhouZhejiang ProvinceChina Qiandongnan Prefecture People’s Hospital KailiGuizhou ProvinceChina Department of Orthopedics Fourth Medical CenterChinese PLA General HospitalBeijingChina

Spinal cord injuries impose a notably economic burden on society,mainly because of the severe after-effects they *** the ongoing development of various therapies for spinal cord injuries,their effectiveness remains ***,a deeper understanding of metabolism has opened up a new therapeutic opportunity in the form of metabolic *** this review,we explore the metabolic changes that occur during spinal cord injuries,their consequences,and the therapeutic tools available for metabolic *** spinal cord metabolism is characterized by independent cellular metabolism and intercellular metabolic ***,spinal cord injury results in metabolic disorders that include disturbances in glucose metabolism,lipid metabolism,and mitochondrial *** metabolic disturbances lead to corresponding pathological changes,including the failure of axonal regeneration,the accumulation of scarring,and the activation of *** rescue spinal cord injury at the metabolic level,potential metabolic reprogramming approaches have emerged,including replenishing metabolic substrates,reconstituting metabolic couplings,and targeting mitochondrial therapies to alter cell *** available evidence suggests that metabolic reprogramming holds great promise as a next-generation approach for the treatment of spinal cord *** further advance the metabolic treatment of the spinal cord injury,future efforts should focus on a deeper understanding of neurometabolism,the development of more advanced metabolomics technologies,and the design of highly effective metabolic interventions.

关键词： axons glycolysis metabolic reprogramming metabolism mitochondria neural regeneration neuroprotection oxidative phosphorylation spinal cord injury therapy

A novel flexible nerve guidance conduit promotes nerve regeneration while providing excellent mechanical properties

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Neural Regeneration Research 2025年第7期20卷 2084-2094页

作者： Tong li Quhan Cheng Jingai Zhang Boxin liu Yu shi Haoxue Wang lijie Huang Su Zhang Ruixin Zhang Song Wang Guangxu Lu Peifu Tang Zhongyang liu Kai Wang Department of Orthopedics the Fourth Medical Center of Chinese PLA General HospitalBeijingChina Department of Training and Sports Medicine Characteristic Medical Center of Chinese People’s Armed Police ForceTianjinChina Medical School of Chinese PLA BeijingChina Key Laboratory of Bioactive Materials for the Ministry of Education College of Life SciencesNankai UniversityTianjinChina Department of Orthopedics Tianjin HospitalTianjinChina Department of Rehabilitation Medicine Er Quan Hospital of PAPWuxiJiangsu ProvinceChina

Autografting is the gold standard for surgical repair of nerve defects>5 mm in length;however,autografting is associated with potential complications at the nerve donor *** an alternative,nerve guidance conduits may be *** ideal conduit should be flexible,resistant to kinks and lumen collapse,and provide physical cues to guide nerve *** designed a novel flexible conduit using electrospinning technology to create fibers on the innermost surface of the nerve guidance conduit and employed melt spinning to align ***,we prepared disordered electrospun fibers outside the aligned fibers and helical melt-spun fibers on the outer wall of the electrospun fiber *** presence of aligned fibers on the inner surface can promote the extension of nerve cells along the *** helical melt-spun fibers on the outer surface can enhance resistance to kinking and compression and provide *** novel conduit promoted nerve regeneration and functional recovery in a rat sciatic nerve defect model,suggesting that it has potential for clinical use in human nerve injuries.

关键词： aligned fibers anti-kinking helical fibers nerve guidance conduit nerve regeneration peripheral nerve injury topological guidance

白芍总苷联合来氟米特治疗类风湿性关节炎及对白细胞介素-1β、白细胞介素-6及肿瘤坏死因子-α的影响

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中国实用医刊 2011年第3期38卷 1-3页

作者：史丽璞朱明慧和雅郑州人民医院风湿免疫科 450003

目的研究白芍总苷联合来氟米特对类风湿性关节炎（RA）患者血清炎性细胞因子的作用及临床疗效。方法将80例患者随机分为四组：甲氨蝶呤（MTX）组、MTX＋白芍总苷（TGP）组、MTX＋来氟米特（LEF）组、MTX＋TGP＋LEF组，每组20例，其中MT... 详细信息

目的研究白芍总苷联合来氟米特对类风湿性关节炎（RA）患者血清炎性细胞因子的作用及临床疗效。方法将80例患者随机分为四组：甲氨蝶呤（MTX）组、MTX＋白芍总苷（TGP）组、MTX＋来氟米特（LEF）组、MTX＋TGP＋LEF组，每组20例，其中MTX组做对照组，分别于治疗前、治疗后12周记录患者疼痛目视模拟测试表（VAS）评分、压痛关节数（TJC）、压痛关节指数（TJI）、肿胀关节数（SJC）、肿胀关节指数（SJI）、健康评估问卷（HAQ）评分；摄双手正位片；测定血清白细胞介素（IL）-1β、IL-6、肿瘤坏死因子-α（TNF-α）水平。结果各组治疗后第12周疗效比较：MTX＋TGP＋LEF组能显著降低血清中升高的IL-1β、IL-6、TNF-α水平。MTX＋TGP＋LEF组达到欧洲抗风湿病联盟有效的患者比例（75％）明显优于MTX＋LEF组（68％）、MTX＋TGP组（63％）、MTX组（63％）。结论①联合应用TGP和LEF治疗活动期RA，近期疗效显著优于LEF、MTX组。②通过下调RA患者炎性细胞因子IL-1β、IL-6及TNF-α水平可增强抗炎作用。

关键词：类风湿性关节炎来氟米特白芍总苷细胞因子

High-selectivity electroreduction of low-concentration CO2 with large concentration fluctuation

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Science China Chemistry 2025年

作者： Mengyu Qi Yanbin Ma Chao Zhang Bingwei li Xueqing Yang Zhaolin shi Simeng liu Changhua An Jiqing Jiao Tongbu Lu MOE International Joint Laboratory of Materials Microstructure Analysis and Testing CenterInstitute for New Energy Materials and Low Carbon TechnologiesSchool of Materials Science and EngineeringSchool of Chemistry and Chemical EngineeringTianjin University of Technology Key Laboratory of Advanced Energy Materials Chemistry (Ministry of Education) Nankai University

Electrochemical CO2reduction reaction（CO2RR） has been regarded as one of the most promising solutions to achieving “zero carbon emission”. In most of the CO2RR-related studies, high-purity CO2has been employed as the feed gas; however, in practice, CO2is generally emitted in low concentrations, so it is of great significance to realize high-selectivity electroreduction of low-concentration CO2with large concentration fluctuation. In this work, we constructed a dual-active-site catalyst and successfully achieved CO2local enrichment and conversion for low-concentration CO2. Operando experiments reveal that the catalyst has one type of site for activating CO2and one type of site for binding the reaction intermediates. The dual-active-site catalyst displays a selectivity for formic acid consistently above 97% over a broad potential window（from-0.9 to-1.6 V ***）. Even when fed with a low-concentration CO2stream（volume ratio from 50% down to 10%）, the dual-active-site catalyst could display high activity and selectivity（>91%）. In particular, the selectivity is still above 85% when the CO2volume ratio is as low as 5%. This work offers a feasible route for converting low-concentration CO2via a synergistic effect for dual-active-site catalysts.

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Identification of a dual functional betulinic acid analog for the treatment of osteoarthritis by phenotypic screening

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Science China life Sciences 2025年第1期68卷 300-302页

作者： Ze-Min lin Mai Xiang Wen-Hui Wei Shu-Hui Fan li Chen Jie Wang Xiao-Qian Yang Chun-Hao Yang shi-Jun He Jian-Ping Zuo Laboratory of Immunopharmacology State Key Laboratory of Drug ResearchShanghai Institute of Materia MedicaChinese Academy of Sciences School of Pharmacy University of Chinese Academy of Sciences School of Pharmacy Nanjing University of Chinese Medicine State Key Laboratory of Drug Research Department of Medicinal ChemistryShanghai Institute of Materia MedicaChinese Academy of Sciences Innovation Research Institute of Traditional Chinese Medicine Shanghai University of Traditional Chinese Medicine Experiment Center for Science and Technology Shanghai University of Traditional Chinese Medicine

Dear Editor, Phenotypic drug discovery, which observes compound effects on physiology,has given way to targeting specific molecules, but has revived by using modern methods to find drugs based on therapeutic outcomes in disease models(Vincent et al., 2022). Osteoarthritis(OA) is a prevalent chronic joint disorder, primarily affecting knee joints, with osteoclast activation due to low-grade inflammation resulting in increased bone resorption and structural changes in the joint(Katz et al.,2021).

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