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World Journal of Orthopedics 2024年第2期15卷 192-200页

作者： Jing-Yi sun Cai Li Feng-ying Du College of Basic Medical Science Qilu Medical UniversityZibo 255300Shandong ProvinceChina Department of Gastroenterological Surgery Shandong Provincial Hospital of Shandong First Medical UniversityJinan 250021Shandong ProvinceChina

The effectiveness of platelet-rich plasma(PRP)for the treatment of Achilles tendon disorders still needs to be evaluated through a series of prospective studies,but genomic analysis can reveal the existence of complementary PRP treatment *** on the 96 platelet activation-related genes in the Kyoto Encyclopedia of Genes and Genomes(KEGG)database,we performed Gene Ontology functional enrichment analysis and KEGG enrichment analysis,pathway correlation analysis,and enrichment mapping to determine the enrichment results of the gene set enrichment analysis and found that the cAMP signalling pathway may be the key to enhancing the effectiveness of PRP *** cAMP signalling pathway interacts with the Rap1 signalling pathway and cGMPPKG signalling pathway to mediate the entire pathophy-siological process of Achilles tendon ***,ADCY1-9 may be the key to the activation of the cAMP signalling *** based on the data in the Gene Expression Omnibus database,it was found that ADCY4 and ADCY7 may be the players that play a major role,associated with the STAT4-ADCY4-LAMA5 axis and the GRbeta-ADCY7-SEMA3C axis,which is expected to be a complementary target for enhancing the efficacy of PRP in the treatment of Achilles tendon disease.

关键词： Platelet-rich plasma Achilles tendon disease cAMP Adenylate cyclase Complementary target

Mitochondrial transplantation confers protection against the effects of ischemic stroke by repressing microglial pyroptosis and promoting neurogenesis

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Neural Regeneration Research 2024年第6期19卷 1325-1335页

作者： Li sun Zhaoyan Zhao Jing Guo Yuan Qin Qian Yu Xiaolong shi Fei Guo Haiqin Zhang Xude sun Changjun Gao Qian Yang Department of Experimental Surgery The Second Affiliated Hospital of Air Force Medical University Department of Anesthesiology The Second Affiliated Hospital of Air Force Medical University

Transferring healthy and functional mitochondria to the lateral ventricles confers neuroprotection in a rat model of ischemia-reperfusion injury. Autologous mitochondrial transplantation is also beneficial in pediatric patients with cardiac ischemia-reperfusion injury. Thus, transplantation of functional exogenous mitochondria may be a promising therapeutic approach for ischemic disease. To explore the neuroprotective effect of mitochondria transplantation and determine the underlying mechanism in ischemic stroke, in this study we established a photo-thrombosis-induced mouse model of focal ischemia and administered freshly isolated mitochondria via the tail vein or to the injury site(in situ). Animal behavior tests, immunofluorescence staining, 2,3,5-triphenyltetrazolium chloride(TTC) staining, mRNA-seq, and western blotting were used to assess mouse anxiety and memory, cortical infarct area, pyroptosis, and neurogenesis, respectively. Using bioinformatics analysis, western blotting, co-immunoprecipitation, and mass spectroscopy, we identified S100 calcium binding protein A9(S100A9) as a potential regulator of mitochondrial function and determined its possible interacting proteins. Interactions between exogenous and endogenous mitochondria, as well as the effect of exogenous mitochondria on recipient microglia, were assessed in vitro. Our data showed that:(1) mitochondrial transplantation markedly reduced mortality and improved emotional and cognitive function, as well as reducing infarct area, inhibiting pyroptosis, and promoting cortical neurogenesis;(2) microglial expression of S100A9 was markedly increased by ischemic injury and regulated mitochondrial function;(3) in vitro, exogenous mitochondria enhanced mitochondrial function, reduced redox stress, and regulated microglial polarization and pyroptosis by fusing with endogenous mitochondria; and(4) S100A9 promoted internalization of exogenous mitochondria by the microglia, thereby amplifying their pro-prolifera

关键词： ischemic stroke microglia mitochondria transplantation neurogenesis pyroptosis

Amitriptyline inhibits NLRP3 inflammasome activation via the ASM/CE pathway in a cell model of NAFLD

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BIOCELL 2024年第5期48卷 759-769页

作者： QIN LIU CHUNYAN NIU QIANG ZHANG shiQIN sun YUE CHEN YONGQIANG shi Shengli Clinical Medical College Fujian Medical UniversityFuzhou350001China Department of Gastroenterology Nanjing Lishui People’s HospitalZhongda Hospital Lishui BranchSoutheast UniversityNanjing211200China Department of Gastroenterology Yancheng Third People’s Hospital(The Yancheng School of Clinical Medicine of Nanjing Medical University)Yancheng224000China Administrative Department of Teaching and Scientific Research Nanjing Lishui People’s HospitalZhongda Hospital Lishui BranchSoutheast UniversityNanjing211200China

Background:Nonalcoholic fatty liver disease(NAFLD)is a global health concern with the acid sphingomyelinase(ASM)/ceramide(CE)pathway and the NOD-like receptor family,pyrin domain-containing protein 3(NLRP3)inflammasome identified as pivotal players in lipid disorders and *** study explores the interaction mechanism between the ASM/CE pathway and NLRP3 in NAFLD cell models,aiming to understand the impact of amitriptyline(Ami),an ASM inhibitor,on lipid deposition and hepatocyte injury by regulating the ASM/CE-NLRP3 ***:HepG2 and HL-7702 cells were exposed to free fatty acids(FFAs)to establish the NAFLD *** cells were divided into 5 groups:control group,model group,Ami group,tumor necrosis factoralpha(TNF-α)group,and Ami+TNF-α*** lipid droplets were visualized using Oil Red O staining,and Western blot analysis quantified ASM,NLRP3,and caspase 1 protein *** linked immunosorbent assay(ELISA)was measured CE and ASM levels,while qRT-PCR assessed mRNA *** apoptotic rate was evaluated by flow cytometry(FCM).Results:Following FFAs incubation,significant increases in ASM and CE levels were observed in HepG2 and HL-7702 cells,accompanied by elevated expression of NLRP3,and caspase 1,and IL-1β.TNF-αtreatment further amplified these *** demonstrated a reduction in lipid deposition,suppressed ASM/CE pathway activation,downregulated NLRP3 and caspase 1 expression,and improved ***,MCC950,a selective inhibitor of the NLRP3,mitigated NLRP3,caspase 1,and IL-1βexpression,alleviating lipid deposition and apoptosis in the NAFLD cell ***:The ASM/CE-NLRP3 pathway in NAFLD cells promotes hepatocyte steatosis,inflammation,and cell *** emerges as a promising therapeutic agent by inhibiting the ASM/CE-NLRP3 pathway,underscoring its potential as a key target for NAFLD treatment.

关键词： Nonalcoholic fatty liver disease Hepatocyte Amitriptyline ASM/CE pathway NLRP3 Nonalcoholic steatohepatitis

Investigating Load Regulation Characteristics of a Wind-PV-Coal Storage Multi-Power Generation System

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Energy Engineering 2024年第4期121卷 913-932页

作者： Zhongping Liu Enhui sun Jiahao shi Lei Zhang Qi Wang Jiali Dong Sinopec Star Co. Ltd.Zhengzhou450000China Hebei Key Laboratory of Low Carbon and High Efficiency Power Generation Technology North China Electric Power UniversityBaoding071003China

There is a growing need to explore the potential of coal-fired power plants(CFPPs)to enhance the utilization rate of wind power(wind)and photovoltaic power(PV)in the green energy *** study developed a load regulation model for a multi-power generation system comprising wind,PV,and coal energy storage using realworld *** power supply process was divided into eight fundamental load regulation scenarios,elucidating the influence of each scenario on load *** the framework of the multi-power generation system with the wind(50 MW)and PV(50 MW)alongside a CFPP(330 MW),a lithium-iron phosphate energy storage system(LIPBESS)was integrated to improve the system’s load regulation *** energy storage operation strategy was formulated based on the charging and discharging priority of the LIPBESS for each basic scenario and the charging and discharging load calculation method of LIPBESS auxiliary *** optimization using the particle swarm algorithm,the optimal capacity of LIPBESS was determined to be within the 5.24-4.88 MWh *** an economic perspective,the LIPBESS operating with CFPP as the regulating power source was 49.1% lower in capacity compared to the renewable energy-based storage mode.

关键词： Wind power coal-fired power PV multi-power generation system lithium-iron phosphate energy storage system

CircR-ZC3HC1 mediates MiR-384-5p/SIRT1 axis to promote neuronal autophagy and relieves ischemic stroke

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BIOCELL 2024年第3期48卷 491-499页

作者： MIN SHEN XIAOMAN XU GUANGLING sun LIANGZHU WANG TAO ying HANG SU WEI WANG QINGHUA CAO ZHEZHE sun Department of Neurology The First Affiliated Hospital of Ningbo UniversityNingbo315010China School of Medicine Ningbo UniversityNingbo315211China

Objective:Circular RNAs(circRNAs)have been shown to involve in pathological processes of ischemic stroke(IS),including *** study was designed to explore the effect of circR-ZC3HC1 on neuronal autophagy in IS and the related ***:Expression of circR-ZC3HC1 in blood samples of IS patients and healthy controls was *** neurons were treated with oxygen and glucose deprivation(OGD)to establish IS in vitro *** expression of LC3 and p62 and the number of autophagosomes were examined to evaluate the autophagy level of OGD induced neurons using western blotting and transmission electron *** apoptosis rate and the expression of cleaved caspase-3,Bax,and Bcl-2 were assessed byﬂow cytometry and western *** binding relationships among circR-ZC3HC1,miR-384-5p,and SIRT1 were predicted and veriﬁ***:Low expression of circR-ZC3HC1 was found in blood samples of IS patients and OGD-treated *** circR-ZC3HC1 or inhibited miR-384-5p expression promoted autophagy and inhibited apoptosis of OGD-treated neurons,which could be reversed by further 3-MA ***,circR-ZC3HC1 targeted miR-384-5p to mediate SIRT1 ***-384-5p overexpression or SIRT1 knockdown in the presence of circR-ZC3HC1 overexpression in OGD-treated neurons lead to reduced autophagy and enhanced ***:Collectively,circR-ZC3HC1 promoted neuronal autophagy to attenuate IS via miR-384-5p/SIRT1 axis.

关键词： Ischemic stroke circR-ZC3HC1 miR-384-5p SIRT1 Autophagy

An amorphous FeMoO_(4) nanorod array enabled high-efficiency oxygen evolution electrocatalysis in alkaline seawater

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Nano Research 2024年第4期17卷 2270-2275页

作者： Jie Tang Shengjun sun Xun He Hui Zhang Chaoxin Yang Min Zhang Meng Yue Hefeng Wang Yuntong sun Yonglan Luo Sulaiman Alfaifi Asmaa Farouk Mohamed S.Hamdy Xuping sun Huiqing Wang Binwu ying Department of Laboratory Medicine West China HospitalSichuan UniversityChengdu 610041China College of Chemistry Chemical Engineering and Materials ScienceShandong Normal UniversityJinan 250014China Institute of Fundamental and Frontier Sciences University of Electronic Science and Technology of ChinaChengdu 610054China Chemistry Department Faculty of ScienceKing Abdulaziz UniversityJeddah 21589Saudi Arabia Department of Chemistry College of ScienceKing Khalid UniversityAbha 61413Saudi Arabia Medical Simulation Centre West China Second University HospitalSichuan UniversityChengdu 610041China

The development of highly efficient and durable oxygen evolution reaction(OER)catalysts for seawater electrolysis is of great importance for ***,an amorphous FeMoO_(4) nanorod array on Ni foam is reported as a highly active OER electrocatalyst in alkaline seawater,requiring only overpotentials of 303 and 332 mV to achieve 100 and 300 mA·cm^(-2),***,it shows strong long-term electrochemical durability for at least 50 h.

关键词： amorphous material FeMoO_(4) nanorod array electrocatalyst seawater electrolysis oxygen evolution reaction

In vivo imaging of the neuronal response to spinal cord injury:a narrative review

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Neural Regeneration Research 2024年第4期19卷 811-817页

作者： Junhao Deng Chang sun ying Zheng Jianpeng Gao Xiang Cui Yu Wang Licheng Zhang Peifu Tang Department of Orthopedics The Fourth Medical Center of Chinese PLA General Hospital National Clinical Research Center for Orthopedics Sports Medicine and Rehabilitation School of Life Sciences Tsinghua University Department of Orthopedics Air Force Medical CenterPLA Medical School of Chinese PLA Institute of Orthopedics The First Medical CenterChinese PLA General Hospital Key Lab of Regenerative Medicine in Orthopedics Key Laboratory of Musculoskeletal Trauma and War Injuries PLA

Deciphering the neuronal response to injury in the spinal cord is essential for exploring treatment strategies for spinal cord injury(SCI).However,this subject has been neglected in part because appropriate tools are *** in vivo imaging and labeling methods offer great potential for observing dynamic neural processes in the central nervous system in conditions of health and *** review first discusses in vivo imaging of the mouse spinal cord with a focus on the latest imaging techniques,and then analyzes the dynamic biological response of spinal cord sensory and motor neurons to *** then summarize and compare the techniques behind these studies and clarify the advantages of in vivo imaging compared with traditional neuroscience ***,we identify the challenges and possible solutions for spinal cord neuron imaging.

关键词： anterior horn neurons calcium imaging central nervous system dorsal horn neurons dorsal root ganglion in vivo imaging neuronal response spinal cord injury spinal cord two-photon microscopy

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Active Micro-Nano-Collaborative Bioelectronic Device for Advanced Electrophysiological Recording

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Nano-Micro Letters 2024年第7期16卷 244-264页

作者： Yuting Xiang Keda shi ying Li Jiajin Xue Zhicheng Tong Huiming Li Zhongjun Li Chong Teng Jiaru Fang Ning Hu Department of Chemistry Zhejiang-Israel Joint Laboratory of Self-Assembling Functional MaterialsZJU-Hangzhou Global Scientific and Technological Innovation CenterZhejiang UniversityHangzhou 310058People’s Republic of China School of Basic Medical Sciences Zhejiang Chinese Medical UniversityHangzhou 310053People’s Republic of China General Surgery Department Children’s HospitalZhejiang University School of MedicineNational Clinical Research Center for Children’s HealthHangzhou 310052People’s Republic of China Department of Lung Transplantation and General Thoracic Surgery The First Affiliated HospitalZhejiang University School of MedicineHangzhou 310003People’s Republic of China Department of Orthopedics The Fourth Affiliated HospitalZhejiang University School of MedicineYiwu 322005People’s Republic of China School of Electronics and Information Technology Sun Yat-Sen UniversityGuangzhou 510006People’s Republic of China Department of Obstetrics and Gynecology The Tenth Affiliated HospitalSouthern Medical UniversityDongguan 523059People’s Republic of China Dongguan Key Laboratory of Major Diseases in Obstetrics and Gynecology Dongguan 523059People’s Republic of China

The development of precise and sensitive electrophysiological recording platforms holds the utmost importance for research in the fields of cardiology and *** recent years,active micro/nano-bioelectronic devices have undergone significant advancements,thereby facilitating the study of *** distinctive configuration and exceptional functionality of these active micro-nano-collaborative bioelectronic devices offer the potential for the recording of high-fidelity action potential signals on a large *** this paper,we review three-dimensional active nano-transistors and planar active micro-transistors in terms of their applications in electroexcitable cells,focusing on the evaluation of the effects of active micro/nano-bioelectronic devices on electrophysiological *** forward to the possibilities,challenges,and wide prospects of active micro-nano-devices,we expect to advance their progress to satisfy the demands of theoretical investigations and medical implementations within the domains of cardiology and neuroscience research.

关键词： Active micro/nano collaborative bioelectronic device Three-dimensional active nano-transistor Planar active microtransistor Electrophysiology

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A Transformer-Based Ensemble Framework for the Prediction of Protein-Protein Interaction Sites

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Research 2024年第2期2023卷 703-718页

作者： Minjie Mou Ziqi Pan Zhimeng Zhou Lingyan Zheng Hanyu Zhang Shuiyang shi Fengcheng Li Xiuna sun Feng Zhu College of Pharmaceutical Sciences The Second Affliated HospitalZhejiang University School of MedicineNational Key Laboratory of Advanced Drug Delivery and Release SystemsZhejiang UniversityHangzhou 310058China Innovation Institute for Artificial Intelligence in Medicine of Zhejiang University Alibaba-Zhejiang University Joint Research Center of Future Digital HealthcareHangzhou 330110China

The identification of protein-protein interaction (PPI) sites is essential in the research of protein function and the discovery of new drugs. So far, a variety of computational tools based on machine learning have been developed to accelerate the identification of PPI sites. However, existing methods suffer from the low predictive accuracy or the limited scope of application. Specifically, some methods learned only global or local sequential features, leading to low predictive accuracy, while others achieved improved performance by extracting residue interactions from structures but were limited in their application scope for the serious dependence on precise structure information. There is an urgent need to develop a method that integrates comprehensive information to realize proteome-wide accurate profiling of PPI sites. Herein, a novel ensemble framework for PPI sites prediction, EnsemPPIS, was therefore proposed based on transformer and gated convolutional networks. EnsemPPIS can effectively capture not only global and local patterns but also residue interactions. Specifically, EnsemPPIS was unique in (a) extracting residue interactions from protein sequences with transformer and (b) further integrating global and local sequential features with the ensemble learning strategy. Compared with various existing methods, EnsemPPIS exhibited either superior performance or broader applicability on multiple PPI sites prediction tasks. Moreover, pattern analysis based on the interpretability of EnsemPPIS demonstrated that EnsemPPIS was fully capable of learning residue interactions within the local structure of PPI sites using only sequence information. The web server of EnsemPPIS is freely available at http://***/ensemppis.

关键词： residue extracting ensemble

Effects of coal-fired power plants on soil microbial diversity and community structures

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Journal of Environmental Sciences 2024年第3期137卷 206-223页

作者： Bowen sun Renbin Zhu Yu shi Wanying Zhang Zeming Zhou Dawei Ma Runfang Wang Haitao Dai Chenshuai Che Institute of Polar Environment&Anhui Key Laboratory of Polar Environment and Global Change School of Earth and Space SciencesUniversity of Science and Technology of ChinaHefei 230026China State Key Laboratory of Crop Stress Adaptation and Improvement School of Life SciencesHenan UniversityKaifeng 450046China State Grid Anhui Electric Power Research Institute Hefei 230601China

Long-term deposition of atmospheric pollutants emitted from coal combustion and their effects on the eco-environment have been extensively studied around coal-fired power ***,the effects of coal-fired power plants on soil microbial communities have received little attention through atmospheric pollutant deposition and ***,we collected the samples of power plant soils(PS),coal-stacking soils(CSS)and agricultural soils(AS)around three coal-fired power plants and background control soils(BG)in Huainan,a typical mineral resource-based city in East China,and investigated the microbial diversity and community structures through a high-throughput sequencing ***-stacking significantly increased(pships between soil microbial communities and long-term disturbances from coal-fired power plants.

关键词： Coal-fired power plants Soil microbial communities Coal-stacking Heavy metals Environmental variables

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