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Kartogenin and Its Application in Regenerative Medicine

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Current Medical Science 2019年第1期39卷 16-20页

作者： Jiang-yu CAI Li ZHANG Jun chen shi-yi chen Department of Sports Medicine Huashan HospitalFudcm UniversityShanghai 200040China Department of Dermatology Huas han HospitalFudcm UniversityShanghai 200040China

Regenerative medicine refers to the possibility of replacing aged/daniaged cells with genetically similar young and functional cells to restore or establish normal ***(KGN),a small heterocyclic,drug-like compound was discovered in 2012,which is strongly associated with regenerative *** has been applied in many regenerative fields,including cartilage regeneration and protection,tendon-bone healing,wound healing,and limb *** could facilitate cartilage repair,promote fonnation of cartilage-like transition zone in tendon-bone junctions,stimulate collagen synthesis for wound healing,and regulate limb development in a coordinated *** the related mechanism,filamin A/CBFβ/RUNX1,Ihh,and TGFβ/Smad pathways have been reported to involve ***,KGN is proven a promising agent in regenerative medicine;however,studies conducted on the effect of KGN are limited to date and not convictive for Iong-term *** studies are recommended to explore the long-term effect and poteniial molecular mechanisms of *** investigations may motivate researchers to expand its applications in different forms and fields.

关键词： kartogenin regenerative medicine mechanism

Bone marrow mesenchymal stem cell-derived exosomal microRNAs target PI3K/Akt signaling pathway to promote the activation of fibroblasts

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World Journal of Stem Cells 2023年第4期15卷 248-267页

作者： Fang-Qi Li Wen-Bo chen Zhi-Wen Luo yi-Sheng chen Ya-ying Sun Xiao-Ping Su Jun-Ming Sun shi-yi chen Department of Sports Medicine Huashan HospitalFudan UniversityShanghai 200040China Guangxi Key Laboratory of Oral and Maxillofacial Rehabilitation and Reconstruction Guangxi Medical UniversityNanning 530021Guangxi Zhuang Autonomous RegionChina Laboratory Animal Center Guangxi Medical UniversityNanning 530021Guangxi Zhuang Autonomous RegionChina

BACKGROUND Fibroblast plays a major role in tendon-bone *** derived from bone marrow mesenchymal stem cells(BMSCs)can activate fibroblasts and promote tendon-bone healing via the contained microRNAs(miRNAs).However,the underlying mechanism is not comprehensively ***,this study aimed to identify overlapped BMSC-derived exosomal miRNAs in three GSE datasets,and to verify their effects as well as mechanisms on *** To identify overlapped BMSC-derived exosomal miRNAs in three GSE datasets and verify their effects as well as mechanisms on *** BMSC-derived exosomal miRNAs data(GSE71241,GSE153752,and GSE85341)were downloaded from the Gene Expression Omnibus(GEO)*** candidate miRNAs were obtained by the intersection of three data *** was used to predict potential target genes for the candidate *** and pathway analyses were conducted using the Gene Ontology(GO)and Kyoto Encyclopedia of Genes and Genomes(KEGG)databases,respectively,by processing data with the *** interconnected genes in the protein-protein interaction(PPI)network were analyzed using Cytoscape ***,wound healing assay,collagen contraction assay and the expression of COL I andα-smooth muscle actin positive were applied to investigate the cell proliferation,migration and collagen *** real-time reverse transcription polymerase chain reaction was applied to determine the cell fibroblastic,tenogenic,and chondrogenic *** Bioinformatics analyses found two BMSC-derived exosomal miRNAs,has-miR-144-3p and hasmiR-23b-3p,were overlapped in three GSE *** network analysis and functional enrichment analyses in the GO and KEGG databases indicated that both miRNAs regulated the PI3K/Akt signaling pathway by targeting phosphatase and tensin homolog(PTEN).In vitro experiments confirmed that miR-144-3p and miR-23b-3p stimulated proliferation,migration and collagen sy

关键词： Exosome MicroRNA Fibroblast Mesenchymal stem cell Tendon-bone healing

Navigability of the Northern Sea Route for Arc7 ice-class vessels during winter and spring sea-ice conditions

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Advances in Climate Change Research 2022年第5期13卷 676-687页

作者： shi-yi chen Stefan KERN Xin-Qing LI Feng-Ming HUI Yu-Fang YE Xiao chenG School of Geospatial Engineering and Science Sun Yat-sen UniversityAnd Southern Marine Science and Engineering Guangdong Laboratory(Zhuhai)Zhuhai 519082China Integrated Climate Data Center(ICDC) Center for Earth System Research and Sustainability(CEN)University of HamburgHamburg D-20144Germany

Sea ice hinders the navigability of the Arctic,especially in winter and ***,three Arc7 ice-class Liquefied Natural Gas carrying vessels safely transited the Northern Sea Route(NSR)without icebreaker assistance in January *** and more Arc7 ice-class vessels are putting into the transit services in the ***,it is necessary to analyze sea-ice conditions and their impact on navigation during wintertime,and the future navigability of Arc7 ice-class vessels along the NSR during winter and *** on sea ice datasets from satellite observations and a model using data assimilation,we explored the sea-ice conditions and their impact during the first three successful commercial voyages through the NSR in *** addition,we analyzed the sea ice variation and estimated navigability for Arc7 ice-class vessels in the NSR from January to June of the years 2021–2050 using future projections of the sea-ice cover by the Coupled Model Inter-comparison Project Phase 6(CMIP6)models under two emission scenarios(SSP2-4.5 and SSP5-8.5).The results reveal lower sea ice thickness and similar sea ice concentration during these three transits relative to the past 42 years(from 1979 to 2020).We found the thickness has a larger impact on the vessels’speeds than sea ice *** likely sea ice thickness played a larger role than the sea ice concentration for the successful transit of the NSR in winter *** projections suggest sea ice thickness will decrease further in most regions of the NSR from January to June under all scenarios enabling increased navigability of the NSR for Arc7 ice-class *** vessels could transit through the NSR from January to June under all scenarios by 2050,while some areas near the coast of East Siberian Sea remain inaccessible for Arc7 ice-class vessels in spring(April and May).These findings can support the strategic planning of shipping along the NSR in winter and spring.

关键词： Northern sea route Arc7 ice-class vessel Sea ice thickness Sea ice concentration Navigability Arctic

Optimized timing of using infliximab in perianal fistulizing Crohn's disease

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World Journal of Gastroenterology 2020年第14期26卷 1554-1563页

作者： Xue-Liang Sun shi-yi chen Shan-Shan Tao Li-Chao Qiao Hong-Jin chen Bo-Lin Yang First Clinical Medical College the Affiliated Hospital of Nanjing University of Chinese MedicineNanjing 210029Jiangsu ProvinceChina Department of Colorectal Surgery Suzhou TCM Hospital Affiliated to Nanjing University of Chinese MedicineSuzhou 215000Jiangsu ProvinceChina Department of Colorectal Surgery The Affiliated Hospital of Nanjing University of Chinese MedicineNanjing 210029Jiangsu ProvinceChina

Infliximab(IFX),as a drug of first-line therapy,can alter the natural progression of Crohn’s disease(CD),promote mucosal healing and reduce complications,hospitalizations,and the incidence of *** fistulas are responsible for the refractoriness of CD and represent a more aggressive *** has been demonstrated as the most effective drug for the treatment of perianal fistulizing ***,a significant proportion of patients only partially respond to IFX,and optimization of the therapeutic strategy may increase clinical *** is a significant association between serum drug concentrations and the rates of fistula *** IFX levels during induction are associated with a complete fistula response in these *** the apparent relapse of perianal fistulizing CD,maintenance therapy with IFX over a longer period seems to be more *** appears that patients without deep remission are at an increased risk of relapse after stopping anti-tumor necrosis factor ***,only patients in prolonged clinical remission should be considered for withdrawal of IFX treatment when biomarker and endoscopic remission is demonstrated,especially when the hyperintense signals of fistulas on T2-weighed images have disappeared on magnetic resonance ***,the optimal timing of IFX use is highly individualized and should be determined by a multidisciplinary team.

关键词： Infliximab Crohn’s disease Perianal fistula Optimization Trough level Deep remission

Species authentication of commercial beef jerky based on PCR-RFLP analysis of the mitochondrial 12S rRNA gene

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Journal of Genetics and Genomics 2010年第11期37卷 763-769页

作者： shi-yi chen yi-Ping Liu Yong-Gang Yao Key Laboratory of Animal Models and Human D&ease Mechanisms of the Chinese Academy of Sciences & Yunnan Province Kunming Institute of Zoology Kunming 650223 China Research Institute of Animal Genetics and Breeding Siehuan Agricultural University Ya'an 625014 China

In this study, we determined species-specific variations by analyzing the mitochondrial 12S rRNA gene sequence variation （-440 bp） in 17 newly obtained sequences and 90 published cattle, yak, buffalo, goat, and pig sequences, which represent 62 breeds and 17 geo- graphic regions. Based on the defined species-specific variations, two endonucleases, Alu I and Bfa I, were selected for species authentication using raw meat/tissue samples and the PCR-RFLP method. Goat and pig were identified using the Alu I enzyme, while cattle, yak, and buffalo were identified by digestion with Bfa I. Our approach had relatively high detection sensitivity of cattle DNA in mixed cattle and yak products, with the lowest detectable threshold equaling 20% of cattle DNA in a mixed cattle/yak sample. This method was successfully used to type commercial beef jerky products, which were produced by different companies utilizing various processing technologies. Our results show that several yak jerky products might be implicated in commercial fraud by using cattle meat instead of yak meat.

关键词： 12S rRNA gene PCR-RFLP meat species identification beefjerky commercial fraud

Long head of biceps tendon transposition for massive and irreparable rotator cuff tears:A systematic review and meta-analysis

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World Journal of Orthopedics 2023年第11期14卷 813-826页

作者： Ren-Wen Wan Zhi-Wen Luo yi-Meng Yang Han-Li Zhang Jia-Ni chen shi-yi chen Xi-Liang Shang Department of Sports Medicine Huashan HospitalFudan UniversityShanghai 200040China

BACKGROUND Superior capsular reconstruction(SCR)with long head of biceps tendon(LHBT)transposition was developed to massive and irreparable rotator cuff tears(MIRCTs);however,the outcomes of this technique remain *** To perform a systematic review of biomechanical outcomes and a meta-analysis of clinical outcomes after LHBT transposition for *** We performed a systematic electronic database search on PubMed,EMBASE,and Cochrane *** of SCR with LHBT transposition were included according to the inclusion and exclusion *** studies were assessed for main results and *** clinical studies were evaluated for quality of *** including study characteristics,cohort demographics,and outcomes were extracted.A meta-analysis was conducted of the clinical *** According to our inclusion and exclusion criteria,a total of six biomechanical studies were identified and reported an overall improvement in subacromial contact pressures and prevention of superior humeral migration without limiting range of motion(ROM)after LHBT transposition for MIRCTs.A total of five clinical studies were included in the meta-analysis of LHBT transposition outcomes,consisting of 253 *** results indicated that compared to other surgical methods for MIRCTs,LHBT transposition had advantages of more significant improvement in ROM(forward flexion mean difference[MD]=6.54,95%confidence interval[CI]:3.07-10.01;external rotation[MD=5.15,95%CI:1.59-8.17];the acromiohumeral distance[AHD][MD=0.90,95%CI:0.21-1.59])and reducing retear rate(odds ratio=0.27,95%CI:0.15-0.48).No significant difference in American Shoulder and Elbow Surgeons score,visual analogue scale score,and University of California at Los Angles score was demonstrated between these two groups for *** In general,SCR with LHBT transposition was a reliable and economical technique for treating MIRCTs,both in terms of biomechanical and clinica

关键词： Massive and irreparable rotator cuff tears Long head of biceps tendon transposition Rotator cuff repair Superior capsular reconstruction

Divergent trajectories of lean vs obese non-alcoholic steatohepatitis patients from listing to post-transplant:A retrospective cohort study

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World Journal of Gastroenterology 2022年第26期28卷 3218-3231页

作者： Fakhar Ali Qazi-Arisar Raj Uchila Catherine chen Cathy Yang shi-yi chen Ravikiran Sindhuvalada Karnam Amirhossein Azhie Wei Xu Zita Galvin Nazia Selzner Leslie Lilly Mamatha Bhat Ajmera Transplant Program Toronto General HospitalUniversity Health NetworkToronto M5G 2N2OntarioCanada Division of Gastroenterology and Hepatology Department of MedicineUniversity of TorontoToronto M5G 2N2OntarioCanada National Institute of Liver and GI Diseases Dow University of Health SciencesKarachi 75330Pakistan Department of Biostatistics Princess Margaret Cancer CenterUniversity Health NetworkToronto M5G 2C1OntarioCanada Division of Biostatistics Dalla Lana School of Public HealthUniversity of TorontoToronto M5G 2C1OntarioCanada

BACKGROUND Non-alcoholic steatohepatitis(NASH)cirrhosis is the second most common indication for liver transplantation(LT).The role of body mass index(BMI)on outcomes of NASH cirrhosis has been *** To compare the longitudinal trajectories of patients with lean vs obese NASH cirrhosis,from listing up to post-transplant,having adjusted their BMI for *** We retrospectively reviewed all adult NASH patients listed for LT in our program from 2012 to ***-Gray Competing Risk analyses and Cox Proportional-Hazard Models were performed to examine the cumulative incidence of transplant and survival outcomes *** Out of 265 NASH cirrhosis listed patients,176 were *** age was 61.0 years;46%were females.111 patients underwent *** robust patients had better waitlist survival[hazard ratio(HR):0.12;95%CI:0.05–0.29,P<0.0001]with higher instantaneous rate of transplant(HR:5.71;95%CI:1.26–25.9,P=0.02).Lean NASH patients had a substantially higher risk of graft loss within 90 d post-LT(1.2%vs 13.8%,P=0.032)and death post-LT(2.4%vs 17.2%,P=0.029).1-3-and 5-year graft survival was poor for lean NASH(78.6%,77.3%and 41.7%vs 98.6%,96%and 85%respectively).Overall patient survival post-LT was significantly worse in lean NASH(HR:0.17;95%CI:0.03–0.86,P=0.0142)with 83%lower instantaneous rate of death in obese *** Although lean NASH is considered to be more benign than obese NASH,our study suggests a paradoxical correlation of lean NASH with waitlist outcomes,and graft and patient survival post-LT.

关键词： Outcomes Frailty Waitlist Liver transplant Survival

Evaluating the Phylogenetic Position of Chinese Tree Shrew(Tupaia belangeri chinensis) Based on Complete Mitochondrial Genome:Implication for Using Tree Shrew as an Alternative Experimental Animal to Primates in Biomedical Research

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Journal of Genetics and Genomics 2012年第3期39卷 131-137页

作者： Ling Xu shi-yi chen Wen-Hui Nie Xue-Long Jiang Yong-Gang Yao Key LaBoratory of Animal Models and Human Disease Mechanisms of the Chinese Academy of Sciences ＆ Yunnan Province Kunming Institute of Zoology Kunming Yunnan 650223 China Graduate School of the Chinese Academy of Sciences Beijing 100039 China Institute of Animal Genetics and Breeding Sichuan Agricultural University Ya＇an Sichuan 625014 China State Key Laboratory of Genetic Resources and Evolution Kunming Institute of Zoology Chinese Academy of Sciences Kunming Yunnan 650223 China

Tree shrew （Tupaia belangeri） is currently placed in Order Scandentia and has a wide distribution in Southeast Asia and Southwest China. Due to its unique characteristics, such as small body size, high brain-to-body mass ratio, short reproductive cycle and life span, and low-cost of maintenance, tree shrew has been proposed to be an alternative experimental animal to primates in biomedical research. However, there are some debates regarding the exact phylogenetic affinity of tree shrew to primates. In this study, we determined the mtDNA entire genomes of three Chinese tree shrews （T. belangeri chinensis） and one Malayan flying lemur （Galeopterus variegatus）. Combined with the published data for species in Euarchonta, we intended to diseen] the phylogenetic relationship among representative species of Dermoptera, Scandentia and Primates. The mtDNA genomes of Chinese tree shrews and Malayan flying lemur shared similar gene organization and structure with those of other mammals. Phylogenetic analysis based on 12 concatenated mitochondrial proteinencoding genes revealed a closer relationship between species of Scandentia and Glires, whereas species of Dermoptera were clustered with Primates. This pattern was consistent with previously reported phylogeny based on mtDNA data, but differed from the one reconstructed on the basis of nuclear genes. Our result suggested that the matrilineal affinity of tree shrew to primates may not be as close as we had thought. The ongoing project for sequencing the entire genome of Chinese tree shrew will provide more information to clarify this important issue.

关键词： Chinese tree shrew mtDNA Phylogeny Animal modeh Flying lemur

Physical exercise reverses immuno-cold tumor microenvironment via inhibiting SQLE in non-small cell lung cancer

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Military Medical Research 2024年第4期11卷 616-619页

作者： Zhi-Wen Luo Ya-ying Sun Wei Xia Jun-ying Xu Dong-Jing Xie Chun-Meng Jiao Ji-Ze Dong Hui chen Ren-Wen Wan shi-yi chen Jie Mei Wen-Jun Mao Department of Sports Medicine Huashan Hospital Affiliated to Fudan UniversityShanghai 200040China Department of Sports Medicine Shanghai General HospitalShanghai 200080China Department of Critical Care Medicine the Affiliated Wuxi People’s Hospital of Nanjing Medical UniversityWuxi 214023JiangsuChina Department of Oncology the Affiliated Wuxi People’s Hospital of Nanjing Medical UniversityWuxi 214023JiangsuChina Guangdong Pharmaceutical University Guangzhou 510006China Institute of Acupuncture Research Institutes of Integrative MedicineFudan UniversityShanghai 200433China Department of Cardiothoracic Surgery the Affiliated Wuxi People’s Hospital of Nanjing Medical UniversityWuxi 214023JiangsuChina

Dear Editor,Physical exercise has been shown to be associated with reduced cancer incidence and cancer-associated mortality[1,2],but the underlying mechanisms are *** regulation has emerged as one of the most prominent mechanisms explaining the effects of exercise on cancer[1,2].Physical exercise primarily lowers blood cholesterol and triglycerides,and protects against cardiovascular diseases[3].However,whether physical exercise can modulate cholesterol metabolism in tumor cells is currently unknown.

关键词： Physical exercise Non-small cell lung cancer(NSCLC) Squalene epoxidase(SQLE) Tumor immune microenvironment(TIME)