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Effects of different Helicobacter pylori culture filtrates on growth of gastric epithelial cells

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World Journal of Gastroenterology 2008年第23期14卷 3745-3749页

作者： Yan-Guo Yan Gang Zhao Jin-Ping Ma shi-rong cai Wen-Hua Zhan Department of General Surgery The Affiliated Hospital of Wuhan University of Science & Technology Wuhan 430064 Hubei Province China The Second Department of General Surgery Guangdong Province Hospital Guangzhou 510080 Guangdong Province China Department of Gastrointestinal and Pancreatic Surgery The First Affiliated Hospital Research Center for Gastric Cancer Sun Yat-sen University Guangzhou 510080 Guangdong Province China

AIM:To study the effects of different Helicobacter pylori(H pylori) culture filtrates on growth of gastric epithelial cells. METHODS:Broth culture filtrates of H pylori were prepared. Gastric epithelial cells were treated with the filtrates,and cell growth was determined by growth curve and flow cytometry. DNA damage of gastric epithelial cells was measured by single-cell microgel electrophoresis. RESULTS:Gastric epithelial cells proliferated actively when treated by CagA-gene-positive broth culture filtrates,and colony formation reached 40%. The number of cells in S phase increased compared to controls. Comet assay showed 41.2% comet cells in GES-1 cells treated with CagA-positive filtrates(P < 0.05) . CONCLUSION:CagA-positive filtrates enhance the changes in morphology and growth characteristics of human gastric epithelial tumor cells. DNA damage maybe one of the mechanisms involved in the growth changes.

关键词： CagA gene Gastric epithelial cell Heliobacter pylori Single cell microgel electrophoresis

Vagina vasorum dissection during D2 lymphadenectomy for gastric carcinoma

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World Journal of Gastroenterology 2007年第12期13卷 1867-1869页

作者： Jian-Jun Peng Yu-Long He Wen-Hua Zhan Ping Xiao shi-rong cai Chang-Hua Zhang Hui Wu Department of Gastrointestinal Surgery The First Affiliated Hospital Sun Yat-Sen University Guangzhou 510080 Guangdong Province China Department of Pathology The First Affiliated Hospital Sun Yat-Sen University Guangzhou 510080 Guangdong Province China

AIM: To explore the relationship between metastasis and vagina vasorum in the progress of gastric carcinoma and to find some facts and references for gastric surgeons. METHODS: One hundred and seven specimens of left or right gastric arteries (55 left and 52 right) were gathered from 59 patients undergoing radical gastrectomy for gastric carcinoma. All the frozen specimens were cut into 3 μm-thick sections and stained with hematoxylin-eosin (HE) and immunohistochemical method separately. Cytokeratin (CK) and mesothelial cells (MC) were stained with immunohistochemical method. Cancer cells inside vagina vasorum were detected and the structure of artery wall was observed under microscope. RESULTS: Metastatic cancer cells or tubercles were found inside vagina vasorum in some stage Ⅲ or Ⅳ specimens, but not in stageⅠor Ⅱ specimens. Tumor cells in vagina vasorum were CK positive in 26 specimens of 14 tumors. Among them, stage Ⅲ was found in 4 specimens of 2 tumors, and stage Ⅳ in 22 specimens of 12 tumors. None of these specimens was positive for MC. The positive rate of CK increased with TNM staging. Compared with the lower part, tumors in the upper and middle parts of stomach were more likely to metastasize into vagina vasorum. CONCLUSION: Vagina vasorum dissection should be performed during D2 lymphadenectomy for TNM stage Ⅲ or Ⅳ gastric carcinoma.

关键词： Gastric carcinoma Radical gastrectomy Vagina vasorum Lymphadenectomy Cytokeratin

(-)-Epigallocatechin-3-gallate inhibits VEGF expression induced by IL-6 via Stat3 in gastric cancer

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World Journal of Gastroenterology 2011年第18期17卷 2315-2325页

作者： Bao-He Zhu Hua-Yun Chen Wen-Hua Zhan Cheng-You Wang shi-rong cai Zhao Wang Chang-Hua Zhang Yu-Long He Department ofGastrointestinal and Pancreatic Surgery First Affiliated HospitalSun Yat-Sen University Gastric Cancer Center of Sun Yat-SenUniversity Guangzhou 510080 Guangdong Province China Department of General Surgery First Affiliated Hospital Shenzhen University Shenzhen518035 Guangdong Province China

AIM: To demonstrate that (-)-Epigallocatechin-3-gallate (EGCG) inhibits vascular endothelial growth factor (VEGF) expression and angiogenesis induced by interleukin-6 (IL-6) via suppressing signal transducer and activator of transcription 3 (Stat3) activity in gastric cancer. METHODS: Human gastric cancer (AGS) cells were treated with IL-6 (50 ng/mL) and EGCG at different concentrations. VEGF, total Stat3 and activated Stat3 protein levels in the cell lyses were examined by Western blotting, VEGF protein level in the conditionedmedium was measured by enzyme-linked immunosorbent assay, and the level of VEGF mRNA was evaluated by reverse transcription polymerase chain reaction (RTPCR). Stat3 nuclear translocation was determined by Western blotting with nuclear extract, and Stat3-DNA binding activity was examined with Chromatin immunoprecipitation (ChIP) assay. IL-6 induced endothelial cell proliferation was measured with 3-[4, 5-dimethylthiazol-2-yl]-2, 5-diphenyl tetrazoliumbromide assay, in vitro angiogenesis was determined with endothelial cell tube formation assay in Matrigel, and IL-6-induced angiogenesis in vitro was measured with Matrigel plug assay. RESULTS: There was a basal expression and secretion of VEGF in AGS cells. After stimulation with IL-6, VEGF expression was apparently up-regulated and a 2.4-fold increase was observed. VEGF secretion in the conditioned medium was also increased by 2.8 folds. When treated with EGCG, VEGF expression and secretion were dose-dependently decreased. IL-6 also increased VEGF mRNA expression by 3.1 folds. EGCG treatment suppressed VEGF mRNA expression in a dose-dependent manner. EGCG dose-dependently inhibited Stat3 activation induced by IL-6, but did not change the total Stat3 expression. When treated with EGCG or AG490, VEGF expressions were reduced to the level or an even lower level in the tumor cells not stimulated with IL-6. However, PD98059 and LY294002 did not change VEGF expression induced by IL-6. EGCG inhibited

关键词： Epigallocatechin-3-gallate Vascular endothelial growth factor Signal transducer and activator of transcription 3 Angiogenesis Gastric cancer

Establishing and clinically validating a machine learning model for predicting unplanned reoperation risk in colorectal cancer

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World Journal of Gastroenterology 2024年第23期30卷 2991-3004页

作者： Li-Qun cai Da-Qing Yang rong-Jian Wang He Huang Yi-Xiong shi Department of Colorectal and Anal Surgery Whenzhou Central HospitalWenzhou 325000Zhejiang ProvinceChina Department of Colorectal and Anorectal Surgery The First Affiliated Hospital of Wenzhou Medical UniversityWenzhou 325000Zhejiang ProvinceChina

BACKGROUND Colorectal cancer significantly impacts global health,with unplanned reoperations post-surgery being key determinants of patient *** predictive models for these reoperations lack precision in integrating complex clinical *** To develop and validate a machine learning model for predicting unplanned reoperation risk in colorectal cancer *** Data of patients treated for colorectal cancer(n=2044)at the First Affiliated Hospital of Wenzhou Medical University and Wenzhou Central Hospital from March 2020 to March 2022 were retrospectively *** were divided into an experimental group(n=60)and a control group(n=1984)according to unplanned reoperation *** were also divided into a training group and a validation group(7:3 ratio).We used three different machine learning methods to screen characteristic variables.A nomogram was created based on multifactor logistic regression,and the model performance was assessed using receiver operating characteristic curve,calibration curve,Hosmer-Lemeshow test,and decision curve *** risk scores of the two groups were calculated and compared to validate the *** More patients in the experimental group were≥60 years old,male,and had a history of hypertension,laparotomy,and hypoproteinemia,compared to the control *** logistic regression analysis confirmed the following as independent risk factors for unplanned reoperation(P<0.05):Prognostic Nutritional Index value,history of laparotomy,hypertension,or stroke,hypoproteinemia,age,tumor-node-metastasis staging,surgical time,gender,and American Society of Anesthesiologists *** operating characteristic curve analysis showed that the model had good discrimination and clinical *** This study used a machine learning approach to build a model that accurately predicts the risk of postoperative unplanned reoperation in patients with colorectal cancer,which can improve tr

关键词： Colorectal cancer Postoperative unplanned reoperation Unplanned reoperation Clinical validation Nomogram Machine learning models

Comprehensive analysis of clinical and biological value of ING family genes in liver cancer

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World Journal of Gastrointestinal Oncology 2024年第6期16卷 2592-2609页

作者： shi-cai Liu School of Medical Information Wannan Medical CollegeWuhu 241002Anhui ProvinceChina

BACKGROUND Liver cancer(LIHC)is a malignant tumor that occurs in the liver and has a high mortality in *** ING family genes were identified as tumor suppressor *** expression of these genes can lead to cell cycle arrest,senescence and/or *** family genes are promising targets for anticancer ***,their role in LIHC is still not well *** To have a better understanding of the important roles of ING family members in *** A series of bioinformatics approaches(including gene expression analysis,genetic alteration analysis,survival analysis,immune infiltration analysis,prediction of upstream microRNAs(miRNAs)and long noncoding RNAs(lncRNAs)of ING1,and ING1-related gene functional enrichment analysis)was applied to study the expression profile,clinical relationship,prognostic significance and immune infiltration of ING in *** relationship between ING family genes expression and tumor associated immune checkpoints was investigated in *** molecular mechanism of ING1 mediated hepatocarcinogenesis was preliminarily *** mRNA/protein expression of different ING family genes in LIHC was analyzed in different databases,showing that ING family genes were highly expressed in *** 47 samples from 366 LIHC patients,the ING family genes were altered at a rate of 13%.By comprehensively analyzing the expression,clinical pathological parameters and prognostic value of ING family genes,ING1/5 was ***1/5 was related to poor prognosis of LIHC,suggesting that they may play key roles in LIHC tumorigenesis and *** of the target miRNAs of ING1 was identified as *** upstream lncRNAs of hsa-miR-214-3p,U91328.1,and HCG17,were *** the same time,we found that the expression of ING family genes was correlated with immune cell infiltration and immune checkpoint *** This study lays a foundation for further research on the potential mechanism and clinical v

关键词： Liver cancer ING family genes Noncoding RNAs Immune cell infiltration Prognosis

Detection of hMSH2 and hMLH1 mutations in Chinese hereditary non-polyposis colorectal cancer kindreds

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World Journal of Gastroenterology 2008年第2期14卷 298-302页

作者： Chang-Hua Zhang Yu-Long He Fang-Jin Wang Wu Song Xi-Yu Yuan Dong-Jie Yang Chuang-Qi Chen shi-rong cai Wen-Hua Zhan Department of Gastrointestinopancreatic Surgery of the First Affiliated Hospital Gastric Cancer Center Sun Yat-Sen University Guangzhou 510080 Guangdong Province China Daan Gene Diagnostic Center of Sun Yat-Sen University Guangzhou 510080 Guangdong Province China

AIM： To establish and validate the mutation testing for identification and characterization of hereditary non-polyposis colorectal cancer （HNPCC） in suspected Chinese patients. METHODS： Five independent Chinese kindreds with HNPCC fulfilling the classical Amsterdam criteria were collected. Genomic DNA was extracted after informed consent was obtained. The coding region of hMSH2 and hMLH1 genes was detected by polymerase chain reaction （PCR） and denaturing high-performance liquid chromatography （DHPLC）. Mutations identified in the proband by DHPLC were directly sequenced using a 377 DNA sequencer, analyzed with a basic local alignment tool （BLAST）, and tested in the corresponding family members by direct DNA sequencing. RESULTS： Mutations were identified in two Chinese HNPCC kindreds. One was the missense mutation of hMSH2 c.1808A→G resulting in Asp 603 Gly identified in the proband of the fifth HNPCC （HNPCCS） kindred. In the HNP5 kindred, three family members were found to have this mutation and two of them had colorectal cancer. The other mutation of hMLH1 c.1882A→G was identified in the HNP2 kindred＇s proband, which might be the nonsense mutation analyzed by BLAST. CONCLUSION： Pedigree investigation and mutation testing of hMSH2 and hMLH1 are the practical methods to identify high-risk HNPCC patients in China.

关键词： Screening Human mutS homology 2 gene Human mutL homology 1 gene Colorectal cancer Heredity

Induced pluripotent stem cells(iPSCs):molecular mechanisms of induction and applications

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Signal Transduction and Targeted Therapy 2024年第5期9卷 2065-2090页

作者： Jonas Cerneckis Hongxia cai Yanhong shi Department of Neurodegenerative Diseases Beckman Research Institute of City of HopeDuarteCA 91010USA Irell&Manella Graduate School of Biological Sciences Beckman Research Institute of City of HopeDuarteCA 91010USA

The induced pluripotent stem cell(iPSC)technology has transformed in vitro research and holds great promise to advance regenerative *** have the capacity for an almost unlimited expansion,are amenable to genetic engineering,and can be differentiated into most somatic cell *** have been widely applied to model human development and diseases,perform drug screening,and develop cell *** this review,we outline key developments in the iPSC field and highlight the immense versatility of the iPSC technology for in vitro modeling and therapeutic *** begin by discussing the pivotal discoveries that revealed the potential of a somatic cell nucleus for reprogramming and led to successful generation of *** consider the molecular mechanisms and dynamics of somatic cell reprogramming as well as the numerous methods available to induce ***,we discuss various iPSC-based cellular models,from mono-cultures of a single cell type to complex three-dimensional organoids,and how these models can be applied to elucidate the mechanisms of human development and *** use examples of neurological disorders,coronavirus disease 2019(COVID-19),and cancer to highlight the diversity of disease-specific phenotypes that can be modeled using iPSC-derived *** also consider how iPSC-derived cellular models can be used in high-throughput drug screening and drug toxicity ***,we discuss the process of developing autologous and allogeneic iPSC-based cell therapies and their potential to alleviate human diseases.

关键词： mechanisms programming holds

Circulating tumor DNA in liquid biopsy: Current diagnostic limitation

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World Journal of Gastroenterology 2024年第15期30卷 2175-2178页

作者： shi-cai Liu School of Medical Information Wannan Medical CollegeWuhu 241002Anhui ProvinceChina

With the rapid development of science and technology,cell-free DNA(cfDNA)is rapidly becoming an important biomarker for tumor diagnosis,monitoring and prognosis,and this cfDNA-based liquid biopsy technology has great potential to become an important part of precision *** is the total amount of free DNA in the systemic circulation,including DNA fragments derived from tumor cells and all other somatic *** cells release fragments of DNA into the bloodstream,and this source of cfDNA is called circulating tumor DNA(ctDNA).cfDNA detection has become a major focus in the field of tumor research in recent years,which provides a new opportunity for non-invasive diagnosis and prognosis of *** this paper,we discuss the limitations of the study on the origin and dynamics analysis of ctDNA,and how to solve these problems in the *** the future faces major challenges,it also con-tains great potential.

关键词： Cell-free DNA Circulating tumor DNA Liquid biopsy Cancer Diagnosis Prognosis

Systemic immune-inflammation index for predicting prognosis of colorectal cancer

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World Journal of Gastroenterology 2017年第34期23卷 6261-6272页

作者： Jian-Hui Chen Er-Tao Zhai Yu-Jie Yuan Kai-Ming Wu Jian-Bo Xu Jian-Jun Peng Chuang-Qi Chen Yu-Long He shi-rong cai Gastrointestinal Surgery Center The First Affiliated HospitalSun Yat-sen University Gastric Cancer Center Sun Yat-sen University

AIM To investigate the clinical significance of preoperative systemic immune-inflammation index(SII) in patients with colorectal cancer(CRC). METHODS A retrospective analysis of 1383 cases with CRC was performed following radical surgery. SII was calculated with the formula SII =(P × N)/L, where P, N, and L refer to peripheral platelet, neutrophil, and lymphocyte counts, respectively. The clinicopathological features and follow-up data were evaluated to compare SII with other systemic inflammation-based prognostic indices such as the neutrophil-lymphocyte ratio(NLR) and platelet-lymphocyte ratio(PLR) in patients with *** The optimal cut-off point for SII was defined as 340. The overall survival(OS) and disease-free survival(DFS) were better in patients with low NLR, PLR, and SII(P < 0.05). The SII was an independent predictor of OS and DFS in multivariate analysis. The area under the receiver-operating characteristics(ROC) curve for SII(0.707) was larger than those for NLR(0.602) and PLR(0.566). In contrast to NLR and PLR, SII could effectively discriminate between the TNM subgroups. CONCLUSION SII is a more powerful tool for predicting survival outcome in patients with CRC. It might assist the identification of high-risk patients among patients with the same TNM stage.

关键词： Colorectal cancer Systemic immune-inflammation index Neutrophil-lymphocyte ratio Plateletlymphocyte ratio Prognosis