检索结果-南通市图书馆

Temporal and Tissue-Specific Expression of Tomato 14-3-3 Gene Family in Response to Phosphorus Deficiency

Pedosphere 2012年第6期22卷 735-745页

作者： XU wei-Feng shi wei-ming YAN Feng State Key Laboratory of Soil and Sustainable Agriculture Institute of Soil ScienceChinese Academy of SciencesNanjing 210008(China) Institute of Crop Science and Plant Breeding Justus-Liebig UniversityLudwigstrasse 2335390 Giessen(Germany)

Plants adapt to phosphorus （P） deficiency through a complex of biological processes and many genes are involved. Tomato （Solanum lycopersicum L. ＇Hezuo906＇） plants were selected to grown hydroponically to study the temporal and spatial gene expression patterns of the 14-3-3 gene family and their roles in response to P deficiency in tomato plants. Using real-time reverse-transcriptase polymerase chain reaction （RT-PCR）, we investigated the expression profiles in different tissues （root, stem and leaf） at short-term and long-term P-deficient stress phases. Results revealed that i） four members of 14-3-3 gene family （TFT1, TFT4, TFT6 and TFTT） were involved in the adaptation of tomato plants to P deficiency, ii） TFT7 responded quickly to P deficiency in the root, while TFT6 responded slowly to P deficiency in the leaf, iii） expression response of TFT4 to P-deficient stress was widely distributed in different tissues （root, stem and leaf） while TFT8 only displayed stem-specific expression, and iv） temporal and tissues-specific expression patterns to P deficiency suggested that isoform specificity existed in tomato 14-3-3 gene family. We propose that TFT7 （one member of e-like group in tomato 14-3-3 family） is the early responsive gene and may play a role in the adaptation of tomato plants to shortterm P deficiency, while TFT6 （one member of non-e group in tomato 14-3-3 family） is the later responsive gene and may play a role in the adaptation of tomato plants to long-term P deficiency.

关键词： isoform specificity mRNA P starvation primer pairs real-time RT-PCR

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Effect of Root Surface Iron Plaque on Se Translocation and Uptake by Fe-Deficient Rice

引用

Pedosphere 2007年第5期17卷 580-587页

作者： ZHOU Xin-Bin shi wei-ming State Key Laboratory of Soil and Sustainable Agriculture Institute of Soil Science Chinese Academy of Sciences Nanjing 210008 （China） College of Resources and Environment Southwest University Chongqing 400716 （China）

Under anaerobic conditions, ferric hydroxide deposits on the surface of rice roots and affects uptake and translocation of certain nutrients. In the present study, rice plants were cultured in Fe-deficient or sufficient solutions and placed in a medium containing selenium （Se） for 2 h. Then, FeSO4 was added at the various concentrations of 0, 10, 40, or 70 mg L-1 to induce varying levels of iron plaque on the root surfaces and subsequent uptake of Se was monitored. The uptake of Se was inhibited by the iron plaque, with the effect proportional to the amount of plaque induced. The activity of cysteine synthase was decreased with increasing amounts of iron plaque on the roots. This may be the important reason for iron plaque inhibition of Se translocation. At each level of iron plaque, Fe-deficient rice had more Se than Fe-sufficient rice. Furthermore, with plaque induced by 20 mg Fe L-1, plants from Fe-deficient media accumulated more Se than those from Fe-sufficient media, as the Se concentration was increased from 10 to 30 or 50 mg L^-1. We found that phytosiderophores, highly effective iron chelating agents, could desorb selenite from ferrihydrite. Root exudates of the Fe-deficient rice, especially phytosiderophores in the exudates, could enhance Se uptake by rice plants with iron plaque.

关键词： iron plaque phytosiderophore rice selenium uptake and translocation

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Numerical analysis of the hydraulic fracture communication modes in fracture-cavity reservoirs

引用

Petroleum Science 2022年第5期19卷 2227-2239页

作者： Jia-wei Kao shi-ming wei Wen-Zhi Wang Yan Jin State Key Laboratory Petroleum Resources and Prospecting China University of PetroleumBeijing102249China Sinopec Research Institute of Petroleum Engineering Beijing102206China CNPC Engineering Technology R&D Company Limited Beijing102206China

The fracture-cavity carbonate reservoirs in the Tahe Oilfield in China are mainly exploited by *** need the hydraulic fractures to communicate with caves to create a flow ***,due to the existence of the fracture-cavity systems,the hydraulic fracture propagation morphology is complicated,while the propagation characteristics are not *** analyze the hydraulic fracture propagation in fracture-cavity carbonate formations,based on the discontinuous discrete fracture model,we developed a solid-seepage-freeflow coupled fracturing model for fracture-cavity formations,which can simulate the complex interaction behavior of fractures and *** on the simulation results,we found the interaction rule between hydraulic fractures and fracture-cavity systems:the stress concentration around caves is the main factor that determines the fracture propagation *** due to stress concentration is usually not conducive to communication,while natural fractures distributed around caves could break the rejection *** the hydraulic energy in the hydraulic fracture can make fracture propagate directly and reduce the influence of *** steering fracture formed by temporary plugging is beneficial to the communication of fracture-cavity systems in the non-principal stress *** to the simulation results of different fracture-cavity characteristics,we raised four optimization communication modes for fracture-cavity carbonate formation to provide references for fracturing optimization design and parameter optimization.

关键词： Fracture-cavity carbonate formation Hydraulic fracture propagation Numerical simulation Fracture-cave communication Discontinuous discrete fracture model

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Expression Patterns of Nine Ammonium Transporters in Rice in Response to N Status

引用

Pedosphere 2012年第6期22卷 860-869页

作者： LI Su-Mei LI Bao-Zhen shi wei-ming State Key Laboratory of Soil and Sustainable Agriculture Institute of Soil ScienceChinese Academy of SciencesNanjing 210008(China) Key Laboratory of Agro-ecological Processes in Subtropical Region Institute of Subtropical AgricultureChinese Academy of SciencesHunan 410125(China)

Nitrogen use efficiency （NUE） was very low in China and a loss of as much as 7070 of the applied nitrogen fertilizers was reported in high-yielding rice fields. In order to investigate the molecular basis of high-affinity ammonium transport or uptake into rice （Oryza sativa L.）, we analyzed the expression profiles of nine ammonium transporters （AMT）, three each of OsAMT1, OsAMT2 and OsAMT3, at two different N requirement stages （young seedling stage and tillering stage） of rice growth as well as the changes in these expression patterns according to external N status using real-time reverse transcription polymerase chain reaction （RT-PCR）. The results suggested that the nine OsAMT genes were expressed in different organs of rice plants, including mature roots, new roots, stems, old leaves and new leaves and that the expression patterns were organ specific and independent of the positions of the corresponding proteins in the phylogenetic tree. OsAMT1;1, 3;2 and 3;3 were expressed in the roots and shoots, primarily old leaves, OsAMT1;2 and 1;3 mainly in the roots, and OsAMT2;1, 2;2, 2;3 and 3;1 mainly in the shoots, primarily in new leaves, and relatively more in the stems than other genes. The expression patterns at the two different N requirement stages were the same; however, at the tillering stage with greater N requirements, the OsAMTs transcript levels were greater than those at the young seedling stage with low N requirements. N starvation for 48 h up-regulated OsAMT1;1, 1;2, 3;1, 3;2, 3;3 and down-regulated OsAMT1;3 mRNA abundance. Following N starvation, NH4＋ and NH4NO3 re-supply down-regulated OsAMT1;2 and 3;3 and up-regulated OsAMT1;3, whereas NO3 re-supply down-regulated OsAMT1;1 and 1;2. These suggested that the organ-specific expression pattern of OsAMT could be regulated by N requirement and external N status.

关键词： external N level gene expression growth stage N requirement plant organ

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Mechanisms of Salt Tolerance in Transgenic Arabidopsis thaliana Carrying a Peroxisomal Ascorbate Peroxidase Gene from Barley

引用

Pedosphere 2008年第4期18卷 486-495页

作者： XU wei-Feng shi wei-ming A. UEDA T. TAKABE State Key Laboratory of Soil and Sustainable Agriculture Institute of Soil Science Chinese Academy of Sciences Nanjing 210008 （China）. Graduate School of the Chinese Academy of Sciences Beijing 100049 （China） Graduate School of Bioagricultural Sciences Nagoya University Nagoya 464-8601 （Japan

Ascorbate peroxidases （APX）, localized in the cytosol, peroxisome, mitochondria, and chloroplasts of plant cells, catalyze the reduction of H2O2 to water by using ascorbic acid as the specific electron donor. To determine the role of peroxisomal type ascorbate peroxidase （pAPX）, an antioxidant enzyme, in protection against salt-induced oxidative stress, transgenic Arabidopsis thaliana plant carrying a pAPX gene （HvAPX1） from barley （Hordeum vulgate L.） was analyzed. The transgenic line pAPX3 was found to be more tolerant to salt stress than the wild type. Irrespective of salt stress, there were no significant differences in Na^＋, K^＋, Ca^2＋, and Mg^2＋ contents and the ratio of K^＋ to Na^＋ between pAPX3 and the wild type. Clearly, the salt tolerance in pAPX3 was not due to the maintenance and reestablishment of cellular ion homeostasis. However, the degree of H2O2 and lipid peroxidation （measured as the levels of malondialdehyde） accumulation under salt stress was higher in the wild type than in pAPX3. The mechanism of salt tolerance in transgenic pAPX3 can thus be explained by reduction of oxidative stress injury. Under all conditions tested, activities of superoxide, glutathione reductase, and catalase were not significantly different between pAPX3 and the wild type. In contrast, the activity of APX was significantly higher in the transgenic plant than in wild type under salt stress. These results suggested that in higher plants, HvAPX1 played an important role in salt tolerance and was a candidate gene for developing salttolerant crop plants.

关键词： ascorbate peroxidase oxidative stress peroxisome soil salinity transgenic Arabidopsis thaliana

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Enhancement of NH_4^+ Uptake by NO_3^- in Relation to Expression of Nitrate-Induced Genes in Rice (Oryza sativa) Roots

引用

Pedosphere 2008年第1期18卷 86-91页

作者： ZHAO Xue-Qiang ZHAO Shou-Ping shi wei-ming State Key Laboratory of Soil and Sustainable Agriculture Institute of Soil Science Chinese Academy of Sciences Nanjing 210008 （China） Graduate School of the Chinese Academy of Sciences Beijing 100049 （China）

This study aimed to survey the expression of genes involved in rice N uptake and aasimilatory network and to understand the potential molecular mechanisms responsible for the NO3^-enhanced NH4^＋ uptake. By using quantitative real-time polymerase chain reaction （PCR）, the genes related to N nutrition, including ammonium transporters （AMTs） and ammonium assimilatory enzymes （GS and GOGAT）, were transcriptionally analyzed in rice plants grown in the absence and presence of NO4^- in the NH4^＋-containing medium. The results showed that NH4^＋ uptake by rice was enhanced by the NO3^- supply to the medium. At the same time and in parallel, the amount of transcripts of seven genes （OsAMT1;1, OsAMT1;2, OsAMT4;1, OsGLNP, OsGLU1, OsGLT1, and OsGLTP） was increased in rice roots, but the expression of two genes （OsGLN1;1 and OsGLN1;P） was decreased and that of OsAMT1;3 remained without change. Up- or downregulation of these genes involved in NH4^＋ uptake and assimilation correlated with the increase in NH4^＋ uptake in the presence of NO3^- in rice roots.

关键词： ammonium gene expression nitrate quantitative real-time PCR rice roots

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

An approximate analytical model for unconventional reservoir considering variable matrix blocks and simultaneous matrix depletion

引用

Petroleum Science 2024年第1期21卷 352-365页

作者： Kai-Xuan Qiu Jia Li Dong Feng shi-ming wei Gang Lei Jiangmen Laboratory of Carbon Science and Technology Jiangmen529100GuangdongChina The Hong Kong University of Science and Technology(Guangzhou) Guangzhou511400GuangdongChina China University of Geosciences(Beijing) Beijing100083China China University of Petroleum(Beijing) Beijing102249China China University of Geosciences(Wuhan) Wuhan430074HubeiChina Shenzhen Research Institute China University of GeosciencesShenzhen518063GuangdongChina

In regard to unconventional oil reservoirs,the transient dual-porosity and triple-porosity models have been adopted to describe the fluid flow in the complex fracture *** has been proven to cause inaccurate production evaluations because of the absence of matrix-macrofracture *** addition,most of the existing models are solved analytically based on Laplace transform and numerical ***,an approximate analytical solution is derived directly in real-time space considering variable matrix blocks and simultaneous matrix *** simplify the derivation,the simultaneous matrix depletion is divided into two parts:one part feeding the macrofractures and the other part feeding the ***,a series of partial differential equations(PDEs)describing the transient flow and boundary conditions are constructed and solved analytically by ***,a relationship between oil rate and production time in real-time space is *** new model is verified against classical analytical *** the microfracture system and matrix-macrofracture communication is neglected,the result of the new model agrees with those obtained with the dual-porosity and triple-porosity model,***,the new model also has an excellent agreement with the numerical *** model is then applied to two actual tight oil wells completed in western Canada sedimentary *** identifying the flow regime,the solution suitably matches the field production data,and the model parameters are *** these output parameters,we can accurately forecast the production and even estimate the petrophysical properties.

关键词： Analytical solution Unconventional reservoir Variable matrix Simultaneous flow

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

In Situ Dissimilatory Nitrate Reduction to Ammonium in a Paddy Soil Fertilized with Liquid Cattle Waste

引用

Pedosphere 2012年第3期22卷 314-321页

作者： LU wei-wei S.RIYA ZHOU Sheng M.HOSOMI ZHANG Hai-Lin shi wei-ming State Key Laboratory of Soil and Sustainable Agriculture Institute of Soil ScienceChinese Academy of SciencesNanjing 210008(China) Graduate University of Chinese Academy of Sciences Beijing 100049(China) Graduate School of Engineering Tokyo University of Agriculture and TechnologyTokyo 184-8588(Japan) Institute of Engineering Tokyo University of Agriculture and TechnologyTokyo 184-8588(Japan) Department of Plant and Soil Sciences Oklahoma State UniversityStillwaterOK 74078(USA)

Most studies on dissimilatory nitrate reduction to ammonium （DNRA） in paddy soils were conducted in the laboratory and in situ studies are in need for better understanding of the DNRA process. In this study, in situ incubations of soil DNRA using ^15N tracer were carried out in paddy fields under conventional water （CW） and low water （LW） managements to explore the potential of soil DNRA after liquid cattle waste （LCW） application and to investigate the impacts of soil redox potential （Eh） and labile carbon on DNRA. DNRA rates ranged from 3.06 to 10.40 mg N kg 1 dry soil d-1, which accounted for 8.55%-12.36% and 3.88% 25.44% of consumption of added NO3-^15N when Eh at 5 cm soil depth ranged from 230 to 414 mV and -225 to -65 mV, respectively. DNRA rates showed no significant difference in paddy soils under two water managements although soil Eh and/or dissolved organic carbon （DOC） were more favorable for DNRA in the paddy soil under CW management 1 d before, or 5 and 7 d after LCW application. Soil DNRA rates were negatively correlated with soil Eh （P 〈 0.05, n = 5） but positively correlated with soil DOC （P 〈 0.05, n - 5） in the paddy soil under LW management, while no significant correlations were shown in the paddy soil under CW management. The potential of DNRA measured in situ was consistent with previous laboratory studies; and the controlling factors of DNRA in paddy soils might be different under different water managements, probably due to the presence of different microfioras of DNRA.

关键词： labile soil carbon livestock waste ^15N labeling soil redox potential

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Overexpression of a Foreign Bt Gene in Cotton Affects the Low-Molecular-weight Components in Root Exudates

引用

Pedosphere 2007年第3期17卷 324-330页

作者： YAN wei-Dong shi wei-ming LI Bao-Hai ZHANG Min State Key Laboratory of Soil and Sustainable Agriculture Institute of Soil Science Chinese Academy of Sciences Nanjing210008 （China）

Most research in the past using genetically modified crops (GM crops) has focused on the ecological safety of foreign gene (i.e., the gene flow), gene products (for example, Bt (Bacillus thuringiensis) protein), and the safety of transgenic food for humans. In this study, changes in both the species and amounts of low-molecular-weight components in cotton (Gossypium hirsutum L.) root exudates after foreign Bt gene overexpression were investigated under different nutritional conditions. Transgenic cotton containing Bt (Bt-cotton), supplemented with all the mineral nutrients, secreted more organic acids than the wild-type cotton (WT). When nitrogen was removed from the full-nutrient solution, the amount of organic acids secretion of Bt-cotton was lesser than that of WT. The roots of the transgenic cotton secreted lesser amounts of amino acids and soluble sugars than the WT roots in the full-nutrient solution. Deficiencies of P and K caused a large increase in the total amino acid and soluble sugar secretions of both Bt-cotton and WT, with larger increases observed in Bt-cotton. Because transferring the foreign Bt gene into cotton can result in alterations in the components of the root exudates, with the effect varying depending on the nutritional status, the cultivation of genetically modified crops, such as Bt-cotton, in soil environments should be more carefully assessed, and the possible effects as a result of the alterations in the root exudate components should be considered.

关键词： gene overexpression low-molecular-weight components nutritional status root exudates transgenic cotton

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Short-Term Responses of Nitrous Oxide Emissions and Concentration Profiles to Fertilization and Irrigation in Greenhouse Vegetable Cultivation

引用

Pedosphere 2012年第6期22卷 764-775页

作者： S. RIYA MIN Ju ZHOU Sheng shi wei-ming M. HOSOMI Graduate School of Engineering Tokyo University of Agriculture and Technology Tokyo 184-8588 (Japan) State Key Laboratory of Soil and Sustainable Agriculture Institute of Soil Science Chinese Academy of Sciences Nanjing 210008 (China) InstitUte of Engineering Tokyo University of Agriculture and Technology Tokyo 184-8588 (Japan)

In vegetable cultivation, the majority of N2O emissions occur after fertilization; it is therefore important to understand any factors contributing to this process. An experiment was conducted to investigate short-term N2O dynamics following topdressing in a greenhouse vegetable field in South China. During two topdressing processes, three different urea-N treatments with irrigation were conducted in May and June in a tomato （Lycopersicum esculentum） cultivation. The N2O fluxes, soil concentration profiles and soil environments at the 0-60 cm depths at 10 cm intervals were measured both immediately prior to and 5 days after topdressing. The N2O fluxes before topdressing ranged from 6.7=1=2.1 to 55.0-4-28.8 μg N m-2 h-1; even higher numbers were recorded in highly fertilized plots. The NO3-N accumulation in the soil caused by vegetable cultivation during the 5 years prior to the start of the experiment, resulted in high background N2O fluxes. One day after topdressing （1 DAT） in May and June, N2O fluxes increased, which coincided with sharp increases in soil N2O concentrations at depths of 2.5 and 15 cm and in NOa-N and NH4＋-N contents at depths of 0-20 cm. From 1 to 5 DAT, fluctuations in the N2O fluxes did not harmonize with the N2O concentrations at a depth of 2.5 cm, which was attributed to different gas diffusion rates at depths of 0-10cm. These results suggested that surface soil N and environmental conditions were crucial for determining the short-term N2O ebullitions during topdressing in greenhouse vegetable cultivation.

关键词： nitrogen application N2O soil profile topdressing vegetable field

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：