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Single-cell transcriptomes and runx2b-/- mutants reveal the genetic signatures of intermuscular bone formation in zebrafish

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National Science Review 2022年第11期9卷 97-107页

作者： Chun-Hong Nie shi-ming wan Yu-Long Chen Ann Huysseune Ya-ming Wu Jia-Jia Zhou Alexandre Wagner Silva Hilsdorf Wei-Min wang Paul Eckhard Witten Qiang Lin Ze-Xia Gao College of Fisheries Key Lab of Freshwater Animal BreedingMinistry of Agriculture and Rural AffairsKey Lab of Agricultural Animal GeneticsBreeding and Reproduction of Ministry of EducationEngineering Research Center of Green Development for Conventional Aquatic Biological Industry in the Yangtze River Economic Belt Ministry of Education Huazhong Agricultural University Hubei Hongshan Laboratory CAS Key Laboratory of Tropical Marine Bio-Resources and Ecology South China Sea Institute of Oceanology Innovation Academy of South China Sea Ecology and Environmental Engineering Chinese Academy of Sciences Department of Biology Ghent University Unit of Biotechnology University of Mogi das Cruzes Guangdong Laboratory for Lingnan Modern Agriculture

Intermuscular bones(IBs) are mineralized spicules, present in the myosepta of many, but not all, teleost species. IBs are often small and sharp, and they consequently limit how the fish can be processed; the IBs may cause injury or trauma if lodged in consumers’ throats or mouths, and therefore affect the appeal of the fish to many consumers. The development of IBs in teleosts is still not fully understood and the molecular basis of IB development remains to be established. Here, the characteristics of IB tissue are evaluated based on single-cell transcriptomics in wild-type zebrafish. The analysis defined 18 distinct cell *** trajectories showed that IBs are derived from tendons and that a core tendon-osteoblast cell lineage is related to IB formation. In particular, the functions of 10 candidate genes were evaluated via CRISPR-Cas9 mutants. Among those, runx2b-/-mutants completely lost IBs, while swimming performance, growth and bone mineral density were not significantly different from runx2b+/+*** single-cell RNA sequencing(sc RNA-seq) analysis in runx2b-/-and runx2b+/+zebrafish revealed the role of osteoblasts in IB formation. In addition, differentially expressed genes were enriched in the transforming growth factor β/bone morphogenetic protein(TGF-β/BMP) pathway after runx2b deletion. This study provides evidence for the crucial role of runx2b regulation in IB formation. Genetic breeding can target runx2b regulation and generate strains of commercial fish species without IBs, which can improve the safe consumption and economic value of many farmed fish species.

关键词： teleosts zebrafish intermuscular bones scRNA-seq tendon-osteoblast cell lineage runx2b genetic breeding

Combining genome-wide association study and transcriptome analysis to identify molecular markers and genetic basis of population-asynchronous ovarian development in Coilia nasus

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Zoological Research 2024年第3期45卷 491-505页

作者： Yue Yu shi-ming wan Cheng-You Huang Shuang-Meng Zhang Ai-Li Sun Jun-Qi Liu Shun-Yao Li Yong-Fu Zhu Shu-Xin Gu Ze-Xia Gao College of Fisheries Hubei Hongshan Laboratory/Key Lab of Freshwater Animal BreedingMinistry of Agriculture and Rural Affairs/Engineering Research Center of Green Development for Conventional Aquatic Biological Industry in the Yangtze River Economic BeltMinistry of EducationHuazhong Agricultural UniversityWuhanHubei 430070China Laboratory for Marine Biology and Biotechnology Qingdao Marine Science and Technology CenterQingdaoShandong 266237China Wuhan Hengyuxin Technology Breeding Professional Cooperative WuhanHubei 430117China Hubei Provincial Aquatic Science Research Institute WuhanHubei 430208China Zhenjiang Jiangzhiyuan Fishery Technology Co. Ltd.ZhenjiangJiangsu 212213China

Coilia nasus,a migratory fish species found in the middle and lower reaches of the Yangtze River and along offshore areas of China,possesses considerable aquacultural and economic ***,the species faces challenges due to significant variation in the gonadal development rate among females,resulting in inconsistent ovarian maturation times at the population level,an extended reproductive period,and limitations on fish growth rate due to ovarian *** the present study,we combined genome-wide association study(GWAS)and comparative transcriptome analysis to investigate the potential single nucleotide polymorphisms(SNPs)and candidate genes associated with population-asynchronous ovarian development in *** of the female population based on whole-genome resequencing yielded 2120695 high-quality SNPs,39 of which were suggestively associated with ovarian *** note,a significant SNP peak on LG21 containing 30 suggestively associated SNPs was identified,with cpne5a determined as the causal gene of the ***,single-marker and haplotype association analyses were performed on cpne5a,revealing four genetic markers(P0.9)significantly associated with the *** transcriptome analysis of precociously and normally maturing individuals screened out 29 and 426 overlapping differentially expressed genes in the brain and ovary,respectively,between individuals of different body *** the GWAS and transcriptome analysis results,this study identified genes and pathways related to hypothalamic-pituitary-gonadal axis hormone secretion,extracellular matrix,angiogenesis,and gap junctions involved in population-asynchronous ovarian *** insights gained from this study provide a basis for a deeper understanding of the molecular mechanisms underlying ovarian development in fish and may facilitate the genetic breeding of *** strains exhibiting population-synchronous ovarian

关键词： Coilia nasus GWAS Transcriptome Ovarian development SNP

Quantum public-key crypto via EPR pairs

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Science China(Physics,Mechanics & Astronomy) 2025年第1期Mechanics & Astronomy) .卷 58-63页

作者： Xiang-Qun Fu Hong-Wei Li Jian-Hong shi Tan Li wan-Su Bao Henan Key Laboratory of Quantum Information and Cryptography Information Engineering University Synergetic Innovation Center of Quantum Information and Quantum Physics University of Science and Technology of China

With the development of quantum computing technology, quantum public-key cryptography is gradually becoming an alternative to the existing classical public-key cryptography. This paper designs a quantum trapdoor one-way function via EPR pairs and quantum measurements. Based on this, a new quantum public-key cryptosystem is presented, which offers forward security,and can resist the chosen-plaintext attack and chosen-ciphertext attack. Compared with the existing quantum public-key cryptos,eavesdropping can be automatically detected in this new quantum public-key cryptosystem under a necessary condition, which is also detailed in the paper.

关键词： quantum public-key crypto trapdoor one-way function EPR pairs

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双区半连续压滤机设计

煤矿机械 2010年第8期31卷 42-43页

作者：史万明吴浩徐州天科机械制造有限公司江苏徐州221004

在煤矿水仓清挖新技术中,煤泥的脱水处理是关键。双区半连续压滤机的设计有效地解决了煤泥的连续压滤脱水问题,同时该产品结构紧凑、体积小、压滤效率高,可以很好地适应井下环境。

关键词：水仓煤泥清挖新技术双区半连续压滤机设计

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海洋潮流能发电装置综述

海洋通报 2016年第6期35卷 601-608页

作者：王世明任万超吕超上海海洋大学工程学院上海201306

介绍了国内外潮流能发电装置发展情况,从装置类型、工作原理和实际应用等方面进行了概括和综述,对比了不同类型潮流能发电装置的能量转换装置结构和载体形式的技术特点,评述了当前潮流能开发中存在的主要问题及未来的发展趋势,指出了当... 详细信息

介绍了国内外潮流能发电装置发展情况,从装置类型、工作原理和实际应用等方面进行了概括和综述,对比了不同类型潮流能发电装置的能量转换装置结构和载体形式的技术特点,评述了当前潮流能开发中存在的主要问题及未来的发展趋势,指出了当前国内潮流能发电装置的研究与国外仍有一定差距,缺少规模化,商业化。

关键词：可再生能源海洋能潮流能潮流发电装置

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ZY-100LB型螺旋扒斗装岩机设计

煤矿机械 2013年第6期34卷 20-21页

作者：史万明张芦岩徐州天科机械制造有限公司江苏徐州221112

目前煤矿广泛应用的立爪式装岩机和挖掘式装岩机,由于固有的结构缺陷,降低了工作效率。螺旋扒斗式装岩机,采用对称截齿螺旋集料,小摆臂扒斗快速扒料,宽铲斗集料的原理,使本机拥有全断面作业,不需主机左右移动、快速、连续装岩的特点,作... 详细信息

目前煤矿广泛应用的立爪式装岩机和挖掘式装岩机,由于固有的结构缺陷,降低了工作效率。螺旋扒斗式装岩机,采用对称截齿螺旋集料,小摆臂扒斗快速扒料,宽铲斗集料的原理,使本机拥有全断面作业,不需主机左右移动、快速、连续装岩的特点,作业效率大幅提高。

关键词：对称截齿螺旋集料小摆臂扒斗宽铲斗集料高效螺旋扒斗装岩机

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水仓清挖机械现状及发展趋势

煤矿机械 2012年第6期33卷 4-6页

作者：史万明张芦岩徐州天科机械制造有限公司江苏徐州221008

简要介绍了当前煤矿用水仓清挖机械的现状和主要优缺点,提出了此类产品的特殊要求和发展趋势。

关键词：水仓特性水仓清挖机械特点现状发展趋势

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高效水仓煤泥固液分离工艺和设备

煤矿机械 2017年第8期38卷 96-97页

作者：史万明徐州天科机械制造有限公司江苏徐州221116

介绍了一种水仓煤泥处理工艺和相应设备,其中煤泥粗分和浓缩集成在一台设备上,以一种简单的方式实现,压滤脱水则研制了一种适应煤泥黏度变化的多功能设备,大幅提高了水仓煤泥的处理效率。

关键词：水仓煤泥处理粗分浓缩压滤

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表面活性剂对金属荧光反应的增敏作用

化学学报 1983年第11期41卷 1029-1037页

作者：史慧明崔万苍王如骥南开大学化学系南开大学化学系天津天津七九届研究生

研究了各种类型的表面活性剂对铪-栎精、锆-栎精、锡-桑色素、镁-8-羟基喹啉-5-磺酸（镁-HQS）、锌-H2QS、镉-H2QS、铽-EDTA-磺基水杨酸体系荧光光谱的影响.结果表明:在适当的表面活性剂存在下,各种配合物的荧光强度均大大增强.确定了... 详细信息

研究了各种类型的表面活性剂对铪-栎精、锆-栎精、锡-桑色素、镁-8-羟基喹啉-5-磺酸（镁-HQS）、锌-H2QS、镉-H2QS、铽-EDTA-磺基水杨酸体系荧光光谱的影响.结果表明:在适当的表面活性剂存在下,各种配合物的荧光强度均大大增强.确定了在有表面活性剂参与下三元配合物的最佳形成条件;以荧光法测定了这些荧光配合物的组成.用相对法测定了有表面活性剂和没有表面活性剂存在时荧光配合物的量子效率,并计算了在激发波长下各自的摩尔吸光系数.发现在有表面活性剂存在时,由于生成了有固定组成的三元离子缔合物,荧光配合物的量子效率和摩尔吸光系数都有不同程度的提高,从而大大增强了它们的荧光强度.讨论了表面活性剂胶束的作用以及表面活性剂的分子结构对荧光反应的影响.指出只有那些分子中带电荷基团与共轭大π键不相邻的表面活性剂才能对荧光反应起增敏作用.利用表面活性剂的增敏作用有可能建立一些高灵敏度的金属荧光分析法.

关键词：荧光反应荧光配合物表面活性剂水泥外加剂三元配合物荧光强度二元配合物荧光光谱桑色素特效试剂离子缔合物 CTMAB 增敏作用金属金属材料