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不同杂交组合肉牛部分肉质指标的测定

西南师范大学学报（自然科学版） 2007年第3期32卷 60-62页

作者：石利香徐恢仲熊廷奎西南大学动物科技学院重庆400716 重庆市丰都县畜牧局重庆丰都408200

选择5个品种(DH、IJ、AB、AG、HF)分别与川南山地黄牛杂交。各取其F_1代育肥牛3头屠宰,测定不同杂交组合肉牛的部分肉质指标的含量,测定结果表明:(1)各组合间大肠菌群和镉的含量为零,水分、灰分、铅、砷、铬指标的含量差异不显著(P>0.05... 详细信息

选择5个品种(DH、IJ、AB、AG、HF)分别与川南山地黄牛杂交。各取其F_1代育肥牛3头屠宰,测定不同杂交组合肉牛的部分肉质指标的含量,测定结果表明:(1)各组合间大肠菌群和镉的含量为零,水分、灰分、铅、砷、铬指标的含量差异不显著(P>0.05),HF组合的汞含量差异显著(P<0.05);(2)所测指标的含量均达到无公害牛肉标准.

关键词：肉牛肉质无公害牛肉

高效液相色谱法测定泡打粉中非食用物质直链烷基苯磺酸钠的方法研究

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中国食品添加剂 2009年第5期20卷 183-186页

作者：石相莉曾雪平林尚鹏桂林市产品质量监督检验所桂林541002 桂林市红星化工有限责任公司桂林541002

建立了泡打粉中LAS的测定方法。样品以水溶解,调节pH值为3,经氯仿提取后用高效液相色谱法测定。讨论了色谱柱、流动相pH值和流速、波长、氯仿纯度对测定所造成的影响。最终确定分析条件为C8(4.6mm×250mm×5μm)柱、流动相甲醇与10mmol/... 详细信息

建立了泡打粉中LAS的测定方法。样品以水溶解,调节pH值为3,经氯仿提取后用高效液相色谱法测定。讨论了色谱柱、流动相pH值和流速、波长、氯仿纯度对测定所造成的影响。最终确定分析条件为C8(4.6mm×250mm×5μm)柱、流动相甲醇与10mmol/L乙酸铵(pH=9)溶液体积比65∶35、流速0.8mL/min、紫外检测器、检测波长225nm,以保留时间定性。对泡打粉样品、加标样品、试剂空白分别进行了测定,结果表明:该方法简便、灵敏准确,可消除泡打粉中糖精钠、香兰素等基质的干扰。

关键词：高效液相色谱法氯仿提取直链烷基苯磺酸钠(LAS) 泡打粉

miRNA-21-5p is an important contributor to the promotion of injured peripheral nerve regeneration using hypoxia-pretreated bone marrow-derived neural crest cells

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Neural Regeneration Research 2025年第1期20卷 277-290页

作者： Meng Cong Jing-Jing Hu Yan Yu Xiao-li li Xiao-Ting Sun li-Ting Wang Xia Wu ling-Jie Zhu Xiao-Jia Yang Qian-Ru He Fei Ding Hai-Yan shi School of Medicine Nantong UniversityNantongJiangsu ProvinceChina Key Laboratory of Neuroregeneration of Jiangsu and Ministry of Education and Coinnovation Center of Neuroregeneration Nantong UniversityNantongJiangsu ProvinceChina Department of Physiology Jiangsu Health Vocational CollegeNanjingJiangsu ProvinceChina Department of Pathology Affiliated Hospital of Nantong UniversityNantongJiangsu ProvinceChina Jiangsu Clinical Medicine Center of Tissue Engineering and Nerve Injury Repair Affiliated Hospital of Nantong UniversityNantongJiangsu ProvinceChina

Our previous study found that rat bone marrow–derived neural crest cells(acting as Schwann cell progenitors)have the potential to promote long-distance nerve ***-based therapy can enhance peripheral nerve repair and regeneration through paracrine bioactive factors and intercellular ***,the complex contributions of various types of soluble cytokines and extracellular vesicle cargos to the secretome remain *** investigate the role of the secretome and extracellular vesicles in repairing damaged peripheral nerves,we collected conditioned culture medium from hypoxia-pretreated neural crest cells,and found that it significantly promoted the repair of sensory neurons damaged by oxygen-glucose *** mRNA expression of trophic factors was highly expressed in hypoxia-pretreated neural crest *** performed RNA sequencing and bioinformatics analysis and found that miR-21-5p was enriched in hypoxia-pretreated extracellular vesicles of neural crest ***,to further clarify the role of hypoxia-pretreated neural crest cell extracellular vesicles rich in miR-21-5p in axonal growth and regeneration of sensory neurons,we used a microfluidic axonal dissociation model of sensory neurons in vitro,and found that hypoxia-pretreated neural crest cell extracellular vesicles promoted axonal growth and regeneration of sensory neurons,which was greatly dependent on loaded ***,we constructed a miR-21-5p-loaded neural conduit to repair the sciatic nerve defect in rats and found that the motor and sensory functions of injured rat hind limb,as well as muscle tissue morphology of the hind limbs,were obviously *** findings suggest that hypoxia-pretreated neural crest extracellular vesicles are natural nanoparticles rich in ***-21-5p is one of the main contributors to promoting nerve regeneration by the neural crest cell *** helps to explain the mechanism of action of the secretome and e

关键词： axotomy cell-free therapy conditioned medium extracellular vesicles hypoxic preconditioning microRNA oxygen-glucose deprivation peripheral nerve injury Schwann cell precursors

HPLC法同时测定不同产地菝葜中5种成分的含量

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化工设计通讯 2022年第11期48卷 237-239页

作者：时向利朱倩江苏康缘医药商业有限公司质量部江苏连云港222000 江苏康缘药业股份有限公司质量部江苏连云港222000

建立HPLC法同时测定菝葜中绿原酸、落新妇苷、黄杞苷、白藜芦醇和异黄杞苷的含量。色谱柱为Kromasil C18(4.6×150mm,3.5μm)柱;流动相为乙腈-0.1%磷酸溶液,梯度洗脱;柱温35℃;流速1.0mL/min;检测波长为303nm。本方法重复性和准确度好,... 详细信息

建立HPLC法同时测定菝葜中绿原酸、落新妇苷、黄杞苷、白藜芦醇和异黄杞苷的含量。色谱柱为Kromasil C18(4.6×150mm,3.5μm)柱;流动相为乙腈-0.1%磷酸溶液,梯度洗脱;柱温35℃;流速1.0mL/min;检测波长为303nm。本方法重复性和准确度好,为完善菝葜的质量标准提供了依据。

关键词： HPLC 菝葜产地含量测定

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天水地区山地辣椒穴盘育苗技术

园艺与种苗 2020年第10期40卷 32-33页

作者：史祥丽杨兵丽武山县生产力促进中心甘肃天水741300 武山县蔬菜研究所甘肃天水741300

为实现天水地区山地辣椒标准化生产,培育和壮大山地辣椒品牌,从集约化育苗与供苗角度出发,探索和总结出一套辣椒穴盘育苗技术。该文从品种选择、材料准备、育苗技术、种苗管理和病虫害防治等方面进行阐述,为实现山地辣椒集约化育苗,发... 详细信息

为实现天水地区山地辣椒标准化生产,培育和壮大山地辣椒品牌,从集约化育苗与供苗角度出发,探索和总结出一套辣椒穴盘育苗技术。该文从品种选择、材料准备、育苗技术、种苗管理和病虫害防治等方面进行阐述,为实现山地辣椒集约化育苗,发展山地辣椒产业提供了技术依据。

关键词：山地辣椒穴盘育苗病虫害

HPLC波长切换法测定不同产地紫菀中芹菜素、槲皮素和紫菀酮的含量

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化工设计通讯 2022年第7期48卷 184-186页

作者：时向利朱倩江苏康缘医药商业有限公司江苏连云港222000 江苏康缘药业股份有限公司江苏连云港222000

建立HPLC波长切换法同时测定紫菀中芹菜素、槲皮素和紫菀酮的含量。色谱柱为Agilent ZORBAX SB-C18(4.6×250mm,5μm)柱;流动相:乙腈-0.1%磷酸溶液,梯度洗脱;柱温40℃;流速1.0mL/min;检测波长:0~30min设为360nm(测定槲皮素和芹菜素),30~... 详细信息

建立HPLC波长切换法同时测定紫菀中芹菜素、槲皮素和紫菀酮的含量。色谱柱为Agilent ZORBAX SB-C18(4.6×250mm,5μm)柱;流动相:乙腈-0.1%磷酸溶液,梯度洗脱;柱温40℃;流速1.0mL/min;检测波长:0~30min设为360nm(测定槲皮素和芹菜素),30~55min设为200nm(测定紫菀酮)。结果表明,芹菜素、槲皮素和紫菀酮分别在8.23~131.71μg/mL(r=0.9999)、11.08~177.29μg/mL(r=0.9998)、12.58~201.29μg/mL(r=1.000)范围内线性关系良好,平均回收率分别为99.53%(RSD=1.51%)、99.38%(RSD=1.68%)、99.75%(RSD=1.74%)。

关键词：紫菀芹菜素槲皮素紫菀酮含量测定

AAV mediated carboxyl terminus of Hsp70 interacting protein overexpression mitigates the cognitive and pathological phenotypes of APP/PS1 mice

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Neural Regeneration Research 2025年第1期20卷 253-264页

作者： Zhengwei Hu Jing Yang Shuo Zhang Mengjie li Chunyan Zuo Chengyuan Mao Zhongxian Zhang Mibo Tang Changhe shi Yuming Xu Department of Neurology The First Affiliated Hospital of Zhengzhou UniversityZhengzhou UniversityZhengzhouHenan ProvinceChina Academy of Medical Sciences of Zhengzhou University ZhengzhouHenan ProvinceChina NHC Key Laboratory of Prevention and treatment of Cerebrovascular Diseases The First Affiliated Hospital of Zhengzhou UniversityZhengzhou UniversityZhengzhouHenan ProvinceChina Henan Key Laboratory of Cerebrovascular Diseases The First Affiliated Hospital of Zhengzhou UniversityZhengzhou UniversityZhengzhouHenan ProvinceChina Institute of Neuroscience Zhengzhou UniversityZhengzhouHenan ProvinceChina Sino-British Research Centre for Molecular Oncology National Centre for International Research in Cell and Gene TherapySchool of Basic Medical SciencesAcademy of Medical SciencesZhengzhou UniversityZhengzhouHenan ProvinceChina Department of Gerontology The First Affiliated Hospital of Zhengzhou UniversityZhengzhou UniversityZhengzhouHenan ProvinceChina

The E3 ubiquitin ligase,carboxyl terminus of heat shock protein 70(Hsp70)interacting protein(CHIP),also functions as a co-chaperone and plays a crucial role in the protein quality control *** this study,we aimed to investigate the neuroprotective effect of overexpressed CHIP on Alzheimer’s *** used an adeno-associated virus vector that can cross the blood-brain barrier to mediate CHIP overexpression in APP/PS1 mouse *** overexpression significantly ameliorated the performance of APP/PS1 mice in the Morris water maze and nest building tests,reduced amyloid-βplaques,and decreased the expression of both amyloid-βand phosphorylated *** also alleviated the concentration of microglia and astrocytes around *** APP/PS1 mice of a younger age,CHIP overexpression promoted an increase in ADAM10 expression and inhibitedβ-site APP cleaving enzyme 1,insulin degrading enzyme,and neprilysin *** of HSP70 and HSP40,which have functional relevance to CHIP,were also *** nuclei transcriptome sequencing in the hippocampus of CHIP overexpressed mice showed that the lysosomal pathway and oligodendrocyte-related biological processes were up-regulated,which may also reflect a potential mechanism for the neuroprotective effect of *** research shows that CHIP effectively reduces the behavior and pathological manifestations of APP/PS1 ***,overexpression of CHIP could be a beneficial approach for the treatment of Alzheimer’s disease.

关键词： adeno-associated virus Alzheimer’s disease APP/PS1 mice carboxyl terminus of Hsp70 interacting protein gene therapy

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松香中铁含量的快速测定

生物质化学工程 2006年第2期40卷 25-26页

作者：石相莉黎孟珠桂林市产品质量监督检验所广西桂林541000 桂林航天工业高等专科学校广西桂林541004

介绍了用分光光度计、KSCN作显色剂快速测定松香中铁含量的方法。该检测方法与文献介绍的其它方法相比较,具有简便、快速、准确、成本低等优点。可满足松香生产厂家日常分析要求。

关键词：松香铁光度法快速测定

Tranylcypromine upregulates Sestrin 2 expression to ameliorate NLRP3-related noise-induced hearing loss

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Neural Regeneration Research 2025年第5期20卷 1483-1494页

作者： Xihang Chen Zhifeng Chen Menghua li Weiwei Guo Shuolong Yuan liangwei Xu Chang lin Xi shi Wei Chen shiming Yang Senior Department of Otolaryngology Head and Neck Surgery Chinese PLA General HospitalChinese PLA Medical SchoolBeijingChina State Key Laboratory of Hearing and Balance Science BeijingChina National Clinical Research Center for Otolaryngologic Diseases BeijingChina Key Laboratory of Hearing Science Ministry of EducationBeijingChina Beijing Key Laboratory of Hearing Impairment Prevention and Treatment BeijingChina Department of Otorhinolaryngology Head and Neck Surgery The First Affiliated Hospital of Fujian Medical UniversityFuzhouFujian ProvinceChina Department of Otolaryngology Head and Neck Surgery The 940th Hospital of Joint Logistics Support Force of Chinese PLALanzhouGansu ProvinceChina Key Laboratory of Tropical Biological Resources of Ministry of Education School of Pharmaceutical SciencesHainan UniversityHaikouHainan ProvinceChina Academician Workstation of Hainan University School of Pharmaceutical SciencesYazhou BaySanyaHainan ProvinceChina

Noise-induced hearing loss is the primary non-genetic factor contributing to auditory ***,there are currently no effective pharmacological interventions for patients with noise-induced hearing ***,we present evidence suggesting that the lysine-specific demethylase 1 inhibitor–tranylcypromine is an otoprotective agent that could be used to treat noise-induced hearing loss,and elucidate its underlying regulatory *** established a mouse model of permanent threshold shift hearing loss by exposing the mice to white broadband noise at a sound pressure level of 120 d B for 4 *** found that tranylcypromine treatment led to the upregulation of Sestrin2(SESN2)and activation of the autophagy markers light chain 3B and lysosome-associated membrane glycoprotein 1 in the cochleae of mice treated with *** noise exposure group treated with tranylcypromine showed significantly lower average auditory brainstem response hearing thresholds at click,4,8,and 16 k Hz frequencies compared with the noise exposure group treated with *** findings indicate that tranylcypromine treatment resulted in increased SESN2,light chain 3B,and lysosome-associated membrane glycoprotein 1 expression after noise exposure,leading to a reduction in levels of 4-hydroxynonenal and cleaved caspase-3,thereby reducing noise-induced hair cell ***,immunoblot analysis demonstrated that treatment with tranylcypromine upregulated SESN2 expression via the autophagy *** treatment also reduced the production of NOD-like receptor family pyrin domaincontaining 3(NLRP3)*** conclusion,our results showed that tranylcypromine treatment ameliorated cochlear inflammation by promoting the expression of SESN2,which induced autophagy,thereby restricting NLRP3-related inflammasome signaling,alleviating cochlear hair cell loss,and protecting hearing *** findings suggest that inhibiting lysine-specific demethylase 1 is a

关键词： 4-hydroxynonenal apoptosis autophagy cleaved caspase-3 inflammation NOD-like receptor family pyrin domain-containing 3(NLRP3) noise-induced hearing loss oxidative stress Sestrin2 tranylcypromine