检索结果-南通市图书馆

Composite Sophora Colon-Soluble Capsule Ameliorates DSS-Induced Ulcerative Colitis in Mice via Gut Microbiota-Derived Butyric Acid and NCR+ ILC3

在线全文

学校读者我要写书评

暂无评论

Chinese Journal of Integrative Medicine 2023年第5期29卷 424-433页

作者： CHEN Ming-jun FENG Yang GAO Lu LIN Ming-xiong WANG shi-da tong Zhan-qi Medical School of Chinese People's Liberation Army Beijing(100853)China Department of Traditional Chinese Medicine The Second Medical Center&National Clinical Research Center of Geriatric DiseasesChinese People's Liberation Army General HospitalBeijing(100853)China Institute of Military Cognition and Brain Sciences Academy of Military Medical SciencesAcademy of Military SciencesBeijing(100850)China

Objective: To investigate the effects of composite Sophora colon-soluble Capsule(CSCC) on gut microbiota-mediated short-chain fatty acids(SCFAs) production and downstream group 3 innate lymphoid cells(ILC3s) of dextran sulfate sodium(DSS)-induced ulcerative colitis(UC) mice model. Methods: The main components of CSCC were analyzed by hybrid ultra-high-performance liquid chromatography ion mobility spectromety quadrupole time-of-flight mass spectrometry(UHPLC-IM-QTOF/MS). Twenty-four male BALB/c mice were randomly divided into 4 groups(n=6) by using a computer algorithm-generated random digital, including control, DSS model, mesalazine, and CSCC groups. A DSS-induced colitis mice model was established to determine the effects of CSCC by recording colonic weight, colonic length, index of colonic weight, and histological colonic score. The variations in ILC3s were assessed by immunofluorescence and flow cytometry. The results of gut microbiota and SCFAs were acquired by 16s r DNA and gas chromatography-mass spectrometry(GC-MS) analysis. The expression levels of NCR^(+)ILC3^(-), CCR6^(+)Nkp46-(Lti) ILC3^(-), and ILCreg-specific markers were detected by enzyme-linked immunosorbent assay, and real-time quantitative polymerase chain reaction and Western blot, respectively. Results: The main components of CSCC were matrine, ammothamnine, Sophora flavescens neoalcohol J, and Sophora oxytol U. After 7 days of treatment, CSCC significantly alleviated colitis by promoting the reproduction of intestinal probiotics manifested as upregulation of the abundance of Bacteroidetes species and specifically the Bacteroidales_S24-7 genus(P<0.05). Among the SCFAs, the content of butyric acid increased the most after CSCC treatment. Meanwhile, compared with the model group, Lti ILC3s and its biomarkers were significantly downregulated and NCR^(+) ILC3s were significantly elevated in the CSCC group(P<0.01). Further experiments revealed that ILC3s were differentiated from Lti ILC3s to NCR^(+)ILC3s, resulting in interleukin-22 production wh

关键词： composite Sophora colon-soluble capsule ulcerative colitis group 3 innate lymphoid cells gut microbiota butyric acid

3,6-dichlorobenzo[b]thiophene-2-carboxylic acid alleviates ulcerative colitis by suppressing mammalian target of rapamycin complex 1 activation and regulating intestinal microbiota

在线全文

学校读者我要写书评

暂无评论

World Journal of Gastroenterology 2022年第46期28卷 6522-6536页

作者： Qiong-Zi He Peng Wei jun-Zhi Zhang tong-tong Liu Kun-Qun shi Huan-Huan Liu Jing-Wei Zhang shi-Jia Liu Affiliated Hospital of Nanjing University of Chinese Medicine Jiangsu Province Hospital of Chinese MedicineNanjing 210029Jiangsu ProvinceChina College of The First Clinical Medicine Nanjing University of Chinese MedicineNanjing 210023Jiangsu ProvinceChina School of Life Science and Technology China Pharmaceutical UniversityNanjing 211198Jiangsu ProvinceChina

BACKGROUND 3,6-dichlorobenzo[b]thiophene-2-carboxylic acid(BT2)is a benzothiophene carboxylate derivative that can suppress the catabolism of branched-chain amino acid(BCAA)-associated mammalian target of rapamycin complex 1(mTORC1)activation.Previous studies have demonstrated the therapeutic effects of BT2 on arthritis,liver cancer,and kidney injury.However,the effects of BT2 on ulcerative colitis(UC)are unknown.AIM To investigate the anti-UC effects of BT2 and the underlying mechanism.METHODS Mouse UC models were created through the administration of 3.5%dextran sodium sulfate(DSS)for 7 d.The mice in the treated groups were administered salazosulfapyridine(300 mg/kg)or BT2(20 mg/kg)orally from day 1 to day 7.At the end of the study,all of the mice were sacrificed,and colon tissues were removed for hematoxylin and eosin staining,immunoblot analyses,and immunohistochemical assays.Cytokine levels were measured by flow cytometry.The contents of BCAAs including valine,leucine,and isoleucine,in mouse serum were detected by liquid chromatography-tandem mass spectrometry,and the abundance of intestinal flora was analyzed by 16S ribosomal DNA sequencing.RESULTS Our results revealed that BT2 significantly ameliorated the inflammatory symptoms and pathological damage induced by DSS in mice.BT2 also reduced the production of the proinflammatory cytokines interleukin 6(IL-6),IL-9,and IL-2 and increased the anti-inflammatory cytokine IL-10 level.In addition,BT2 notably improved BCAA catabolism and suppressed mTORC1 activation and cyclooxygenase-2 expression in the colon tissues of UC mice.Furthermore,highthroughput sequencing revealed that BT2 restored the gut microbial abundance and diversity in mice with colitis.Compared with the DSS group,BT2 treatment increased the ratio of Firmicutes to Bacteroidetes and decreased the abundance of Enterobacteriaceae and Escherichia-shigella.CONCLUSION Our results indicated that BT2 significantly ameliorated DSS-induced UC and that the latent mechanism involved the suppression of BCAA-as

关键词： 3,6-dichlorobenzo[b]thiophene-2-carboxylic acid Ulcerative colitis Mechanistic target of rapamycin complex 1 Intestinal flora Dextran sodium sulfate Cyclooxygenase-2

Hydrogen-rich water ameliorates neuropathological impairments in a mouse model of Alzheimer's disease through reducing neuroinflammation and modulating intestinal microbiota

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Neural Regeneration Research 2022年第2期17卷 409-417页

作者： Yi-tong Lin Qing-Qing shi Lei Zhang Cai-Ping Yue Zhi-jun He Xue-Xia Li Qian-jun He Qiong Liu Xiu-Bo Du College of Life Sciences and Oceanography Guangdong Provincial Key Laboratory for Plant EpigeneticsShenzhen UniversityShenzhenGuangdong ProvinceChina Guangdong Provincial Key Laboratory of Biomedical Measurements and Ultrasound Imaging National-Regional Key Technology Engineering Laboratory for Medical UltrasoundSchool of Biomedical EngineeringHealth Science CenterShenzhen UniversityShenzhenGuangdong ProvinceChina Shenzhen Bay Laboratory ShenzhenGuangdong ProvinceChina

Hydrogen exhibits the potential to treat Alzheimer's disease. Stereotactic injection has been previously used as an invasive method of administering active hydrogen, but this method has limitations in clinical practice. In this study, triple transgenic(3×Tg) Alzheimer's disease mice were treated with hydrogen-rich water for 7 months. The results showed that hydrogen-rich water prevented synaptic loss and neuronal death, inhibited senile plaques, and reduced hyperphosphorylated tau and neurofibrillary tangles in 3×Tg Alzheimer's disease mice. In addition, hydrogen-rich water improved brain energy metabolism disorders and intestinal flora imbalances and reduced inflammatory reactions. These findings suggest that hydrogen-rich water is an effective hydrogen donor that can treat Alzheimer's disease. This study was approved by the Animal Ethics and Welfare Committee of Shenzhen University, China(approval No. AEWC-20140615-002) on june 15, 2014.

关键词： Alzheimer's disease amyloid-β anti-inflammation bioenergetics gut microbiota hydrogen therapy neurodegenerative disease neurofibrillary tangles

Recent advances in the development of protein–protein interactions modulators:mechanisms and clinical trials

在线全文

学校读者我要写书评

暂无评论

Signal Transduction and Targeted Therapy 2020年第1期5卷 710-732页

作者： Haiying Lu Qiaodan Zhou jun He Zhongliang Jiang Cheng Peng Rongsheng tong Jianyou shi Personalized Drug Therapy Key Laboratory of Sichuan Province Department of PharmacySichuan Academy of Medical Science&Sichuan Provincial People’s HospitalSchool of MedicineUniversity of Electronic Science and Technology of China610072 ChengduChina Department of Ultrasonic Sichuan Academy of Medical Science&Sichuan Provincial People’s Hospital610072 ChengduChina Cancer Center West China HospitalSichuan University and Collaborative Innovation Center for Biotherapy610041 SichuanChina Miller School of Medicine University of MiamiMiamiFL 33136USA The Ministry of Education Key Laboratory of Standardization of Chinese Herbal Medicines of Ministry State Key Laboratory Breeding Base of Systematic ResearchDevelopment and Utilization of Chinese Medicine ResourcesPharmacy CollegeChengdu University of Traditional Chinese Medicine611137 ChengduChina

Protein–protein interactions(PPIs)have pivotal roles in life processes.The studies showed that aberrant PPIs are associated with various diseases,including cancer,infectious diseases,and neurodegenerative diseases.Therefore,targeting PPIs is a direction in treating diseases and an essential strategy for the development of new drugs.In the past few decades,the modulation of PPIs has been recognized as one of the most challenging drug discovery tasks.In recent years,some PPIs modulators have entered clinical studies,some of which been approved for marketing,indicating that the modulators targeting PPIs have broad prospects.Here,we summarize the recent advances in PPIs modulators,including small molecules,peptides,and antibodies,hoping to provide some guidance to the design of novel drugs targeting PPIs in the future.

关键词： drugs clinical diseases

Spontaneous local redox reaction to passivate CNTs as lightweight current collector for high energy density lithium ion batteries

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Journal of Energy Chemistry 2023年第5期80卷 553-561,I0013页

作者： Chao Lv Zhen tong shi-Yuan Zhou Si-Yu Pan Hong-Gang Liao Yao Zhou jun-Tao Li College of Energy Xiamen UniversityXiamen 361005FujianChina State Key Laboratory of Physical Chemistry of Solid Surfaces College of Chemistry and Chemical EngineeringXiamen UniversityXiamen 361005FujianChina

Extensive usage of highly conductive carbon materials with large specific surface area(e.g.,carbon nanotubes,CNTs)in lithium ion batteries(LIBs),especially as current collector of anodes,suffers from low initial coulombic efficiency(ICE),large interfacial resistance,and severe embrittlement,as the large specific surface area often results in severe interfacial decomposition of the electrolyte and the formation of thick and fluffy solid electrolyte interphase(SEI)during cycling of LIBs.Herein,we demonstrate that when the CNT-based current collector and Na foil(which are being stacked intimately upon each other)are being placed in Na+-based organic electrolyte,local redox reaction between the Na foil and the electrolyte would occur spontaneously,generating a thin and homogeneous NaF-based passivating layer on the CNTs.More importantly,we found that owing to the weak solvation behaviors of Na+in the organic electrolyte,the resulting passivation layer,which is rich in NaF,is thin and dense;when used as the anode current collector in LIBs,the pre-existing passivating layer can function effectively in isolating the anode from the solvated Li+,thus suppressing the formation of bulky SEI and the destructive intercalation of solvated Li+.The relevant half-cell(graphite as anode)exhibits a high ICE of 92.1%;the relevant pouch cell with thus passivated CNT film as current collectors for both electrodes(LiCoO_(2)as cathode,graphite as anode)displays a high energy density of 255 Wh kg^(-1),spelling an increase of 50%compared with that using the conventional metal current collectors.

关键词： Lightweight current collector Passivating layer Initial coulombic efficiency High energy density storage

Lightweight epoxy-based abradable seal coating with high bonding strength

在线全文

学校读者我要写书评

暂无评论

Journal of Materials Science & Technology 2021年第10期69卷 129-137页

作者： Yun-Qi tong Qiu-Sheng shi Mei-jun Liu Guang-Rong Li Chang-Jiu Li Guan-jun Yang State Key Laboratory for Mechanical Behavior o f Materials School of Materials Science and EngineeringXi'an Jiaotong UniversityXi'an710049China

To gain high efficiency and low fuel consumption, aluminum-based abradable seal coatings had been widely used in the compressor casing of aero engines or gas turbines owing to the low elastic modulus. However, the adhesive transfer phenomenon frequently occurs when the radial rubbing generates between titanium alloy blade tips and aluminum-based coating. It tends to increase scratch force and results in blades vibration and even engine jam. To eliminate this problem, a new lightweight epoxy-based abradable seal coating with high bonding strength was developed by an effective and porosity controllable mixing process that distributes spherical pores uniformly in the continuous matrix. A lightweight coating of 63% porosity with a hardness of 33.1(HR15 Y) can be reached when the content of hollow microspheres is 31 wt.%. The coating density is 0.5 g/cm3 and the bonding strength is as high as 18 MPa.The performance of the epoxy-based coating is comprehensively better than aluminum-based coatings in five essential indices. This study is expected to provide a new technical path for obtaining high-quality abradable seal coatings to guarantee the efficient and safe operation of compressors.

关键词： Epoxy-based seal coating Porosity HR15Y Lightweight High bonding strength

Meta-analysis with zero-event studies:a comparative study with application to COVID-19 data

在线全文

学校读者我要写书评

暂无评论

Military Medical Research 2022年第1期9卷 126-137页

作者： Jia-Jin Wei En-Xuan Lin Jian-Dong shi Ke Yang Zong-Liang Hu Xian-Tao Zeng Tie-jun tong department of Mathematics Hong Kong Baptist UniversityHong KongChina Shenzhen Research Institute of Big Data ShenzhenChina College of Mathematics and Statistics Shenzhen UniversityShenzhenChina Center for Evidence-Based and Translational Medicine Zhongnan Hospital of Wuhan UniversityWuhanChina.

Background:Meta-analysis is a statistical method to synthesize evidence from a number of independent studies,including those from clinical studies with binary outcomes.In practice,when there are zero events in one or both groups,it may cause statistical problems in the subsequent analysis.Methods:In this paper,by considering the relative risk as the effect size,we conduct a comparative study that consists of four continuity correction methods and another state-of-the-art method without the continuity correction,namely the generalized linear mixed models(GLMMs).To further advance the literature,we also introduce a new method of the continuity correction for estimating the relative risk.Results:From the simulation studies,the new method performs well in terms of mean squared error when there are few studies.In contrast,the generalized linear mixed model performs the best when the number of studies is large.In addition,by reanalyzing recent coronavirus disease 2019(COVID-19)data,it is evident that the double-zero-event studies impact the estimate of the mean effect size.Conclusions:We recommend the new method to handle the zero-event studies when there are few studies in a meta-analysis,or instead use the GLMM when the number of studies is large.The double-zero-event studies may be informative,and so we suggest not excluding them.

关键词： Continuity correction Coronavirus disease 2019 data Meta-analysis Relative risk Zero-event studies

Denoising Autoencoder, A Deep Learning Algorithm, Aids the Identification of A Novel Molecular Signature of Lung Adenocarcinoma

在线全文

学校读者我要写书评

暂无评论

Genomics, Proteomics & Bioinformatics 2020年第4期18卷 468-480页

作者： jun Wang Xueying Xie junchao shi Wenjun He Qi Chen Liang Chen Wanjun Gu tong Zhou Department of Thoracic Surgery Jiangsu Province People’s Hospital and the First Affiliated Hospital of Nanjing Medical UniversityNanjing 210029China State Key Laboratory of Bioelectronics School of Biological Sciences and Medical EngineeringSoutheast UniversityNanjing 210096China Department of Physiology and Cell Biology University of NevadaReno School of MedicineRenoNV 89557USA State Key Lab of Respiratory Disease Guangzhou Medical UniversityGuangzhou 510000China

Precise biomarker development is a key step in disease management. However, most of the published biomarkers were derived from a relatively small number of samples with supervised approaches. Recent advances in unsupervised machine learning promise to leverage very large datasets for making better predictions of disease biomarkers. Denoising autoencoder(DA) is one of the unsupervised deep learning algorithms, which is a stochastic version of autoencoder techniques. The principle of DA is to force the hidden layer of autoencoder to capture more robust features by reconstructing a clean input from a corrupted one. Here, a DA model was applied to analyze integrated transcriptomic data from 13 published lung cancer studies, which consisted of 1916 human lung tissue samples. Using DA, we discovered a molecular signature composed of multiple genes for lung adenocarcinoma(ADC). In independent validation cohorts, the proposed molecular signature is proved to be an effective classifier for lung cancer histological subtypes. Also, this signature successfully predicts clinical outcome in lung ADC, which is independent of traditional prognostic factors. More importantly, this signature exhibits a superior prognostic power compared with the other published prognostic genes. Our study suggests that unsupervised learning is helpful for biomarker development in the era of precision medicine.

关键词： Denoising autoencoder Unsupervised learning Lung cancer Prognosis Molecular signature

A Novel Mutation of Mitochondrial T14709C Causes Myoclonic Epilepsy with Ragged Red Fibers Syndrome in a Chinese Patient

在线全文

学校读者我要写书评

暂无评论

Chinese Medical Journal 2018年第13期131卷 1569-1574页

作者： Rui Ban jun-Hong Guo Chuan-Qiang Pu Qiang shi Hua-Xu Liu Yu-tong Zhang Department of Neurology Chinese People's Liberation Army General Hospital Beijing 100853 China Department of Neurology School of Medicine Nankai University Tianjin 300071 China Department of Neurology The First Hospital of Shanxi Medical University Taiyuan Shanxi 030001 China

Background： Myoclonic epilepsy with ragged red fibers （MERRF） syndrome is characterized by myoclonus, generalized epilepsy, cerebellar ataxia, and ragged red fibers （RRFs） in the muscle. T-to-C transition at nucleotide position 14709 in the mitochondrial tRNA glutamic acid （tRNA^Gla） gene has previously been associated with maternally inherited diabetes and deathess. However, the association between MERRF and mitochondrial T14709C mutation （m.TI4709C） has never been reported before. Methods： Clinical information of a 17-year-old patient was collected; muscle biopsy and next-generation sequencing （NGS） of whole mitochondrial and neuromuscular disease panel were then conducted. Finally, sanger sequencing was carried out to confirm the mutations. Results： The patient presented a typical MERRF phenotype with muscle weakness, epileptic seizure, clonic episodes, cerebellar ataxia, and spinal scoliosis. Muscle biopsy showed RRFs which indicated abnormal mitochondrial functions. NGS of whole mitochondrial gene revealed m.TI4709C mutation, confirmed by Sanger sequencing. Conclusion： We present a sporadic patient with typical MERRF presentation carrying the mutation ofm.T14709C, which expanded the spectrum of re.T14709C.

关键词： m.TI4709C Myoclonic Epilepsy with Ragged Red Fibers Syndrome： Novel Mutation