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Effects of miR-200c on the migration and invasion abilities of human prostate cancer Du145 cells and the corresponding mechanism

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Frontiers of Medicine 2014年第4期8卷 456-463页

作者： Runlin shi Haibing Xiao Tao Yang Lei chang Yuanfeng Tian Bolin Wu Hua xu Department of Urology Tongji Hospital Tongji Medical College Huazhong University of Science and Technology Wuhan 430030 China

microRNAs （miRNAs） have played a key role in human tumorigenesis, tumor progression, and metastasis. On the one hand, miRNAs are aberrantly expressed in many types of human cancer; on the other hand, miRNAs can function as tumor suppressors or oncogenes that target many cancer-related genes. This study aimed to investigate the effects of miRNA-200c （miR-200c） on the biological behavior and mechanism of proliferation, migration, and invasion in the prostate cancer cell line Du145. In this study, Du145 cells were transfected with miR-200c mimics or negative control miR-NC by using an X-tremeGENE siRNA transfection reagent. The relative expression of miR-200c was measured by RT-PCR. The proliferation, migration, and invasion abilities of Du145 cells were detected by CCK8 assays, migration assays and invasion assays, respectively. The expressions of ZEB1, E-cadherin, and vimentin were observed by western blot. Results showed that DU145 cells exhibited a high expression of miR-200e compared with immortalized normal prostate epithelial cell RWPE-1. Du145 cells were then transfected with miR-200c mimics and displayed lower abilities of proliferation, migration, and invasion than those transfected with the negative control. The protein levels of ZEB1 and vimentin were expressed at a low extent in Du145 cells, which were transfected with miR-200c mimics; by contrast, E-cadherin was highly expressed. Hence, miR-200c could significantly inhibit the proliferation of the prostate cancer cell line Du145; likewise, miR- 200c could inhibit migration and invasion by epithelial-mesenchymal transition.

关键词： miR-200c proliferation migration invasion prostate cancer Du145 cell ZEB1

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嗜酸性粒细胞浸润与慢性鼻窦炎伴鼻息肉患者临床特征及IL-17表达变化的关系

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中国现代医学杂志 2024年第1期34卷 84-88页

作者：庄汉侍孝红徐金葛唱于海峰张其昌徐州医科大学附属宿迁医院(南京鼓楼医院集团宿迁医院)耳鼻咽喉科江苏宿迁223800 徐州医科大学附属宿迁医院(南京鼓楼医院集团宿迁医院)病理科江苏宿迁223800

目的探究嗜酸性粒细胞(EOS)浸润与慢性鼻窦炎伴鼻息肉患者临床特征及IL-17表达变化的关系。方法选取2020年1月—2022年10月徐州医科大学附属宿迁医院收治的100例慢性鼻窦炎伴鼻息肉患者。根据患者是否发生EOS浸润将患者分为对照组(55例... 详细信息

目的探究嗜酸性粒细胞(EOS)浸润与慢性鼻窦炎伴鼻息肉患者临床特征及IL-17表达变化的关系。方法选取2020年1月—2022年10月徐州医科大学附属宿迁医院收治的100例慢性鼻窦炎伴鼻息肉患者。根据患者是否发生EOS浸润将患者分为对照组(55例,未发生EOS浸润)和观察组(45例,发生EOS浸润)。患者均行鼻内镜检查,并留取鼻息肉组织待用。检测患者鼻息肉组织及外周静脉血血清白细胞介素-17(IL-17)水平;对患者行鼻内镜及CT检查,采用Lund-Kennedey及Lund-Mackey评分对患者病变程度及病变范围进行评估;收集患者临床资料,比较其差异,并对可能影响患者发生EOS浸润的因素进行分析,探究EOS浸润与病灶组织及IL-17表达变化的相关性。结果两组患者的年龄、性别构成、病程、中性粒细胞百分比、淋巴细胞百分比及患者病灶分型比较,差异无统计学意义(P>0.05);观察组鼻内镜检查息肉、水肿及鼻漏方面Lund-Kennedey评分与对照组比较,差异均无统计学意义(P>0.05);观察组CT检查结合LundMackey评分在后组筛窦、鼻道窦口复合体、蝶窦及蝶筛隐窝部位的评分均高于对照组,上颌窦评分低于对照组(P0.05);观察组鼻息肉组织及血清IL-17水平高于对照组(P<0.05)。血清IL-17水平、鼻息肉组织IL-17水平、蝶筛隐窝、蝶窦病变、鼻道窦口复合体、后组筛窦与EOS浸润均呈正相关(r=0.859、0.904、0.955、0.680、0.281和0.421,均P=0.000);上颌窦病变与EOS浸润呈负相关(r=-0.700,P=0.000);鼻漏评分与EOS浸润无相关性(r=0.147,P=0.073)。结论慢性鼻窦炎伴鼻息肉发生EOS浸润时,其病灶组织及血清IL-17水平异常改变,且血清IL-17水平、鼻息肉组织IL-17水平、鼻窦病变情况与EOS浸润相关,该研究有助于揭示慢性鼻窦炎伴鼻息肉的发病机制,并为治疗提供新思路,提高诊断和治疗效果,以改善患者生活质量。

关键词：慢性鼻窦炎伴鼻息肉嗜酸性粒细胞浸润临床特征白细胞介素-17

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复合盐碱胁迫对不同谷子品种萌发期的影响及耐盐碱品种筛选

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作物杂志 2024年第3期 207-215页

作者：刘莹尹泽群吴柏辰徐明丽刘畅石慧姝庞博苗兴芬黑龙江八一农垦大学农学院黑龙江大庆163319 黑龙江省农业科学院作物资源研究所黑龙江大庆163319

为了探究复合盐碱胁迫对不同谷子品种萌发的影响,对100份谷子品种进行研究,选定2种中性盐(Na_(2)SO_(4),NaCl)和2种碱性盐(NaHCO_(3),Na_(2)CO_(3)),按4:1:4:1(摩尔比)混合,随机选取5个供试品种进行耐盐碱性的浓度筛选,设0、20、40、60... 详细信息

为了探究复合盐碱胁迫对不同谷子品种萌发的影响,对100份谷子品种进行研究,选定2种中性盐(Na_(2)SO_(4),NaCl)和2种碱性盐(NaHCO_(3),Na_(2)CO_(3)),按4:1:4:1(摩尔比)混合,随机选取5个供试品种进行耐盐碱性的浓度筛选,设0、20、40、60、80、100、120、150 mmol/L共8个处理,研究谷子品种萌发期盐碱胁迫条件下发芽率以及对根、芽的影响。结果表明,80 mmol/L的盐碱溶液是筛选谷子萌发期耐盐碱的最适浓度;在80 mmol/L盐碱浓度下,对100份谷子品种萌发期的耐盐碱性进行了综合评价,结果显示,各品种指标之间差异达到显著或极显著水平,各性状的变异系数从小到大为相对发芽率、相对发芽指数、相对芽长、相对芽干重、相对芽鲜重、相对发芽势、相对根长、相对根鲜重、相对根干重;根据综合评分值(D值),将100份谷子品种分为4个耐盐碱级别,分别为极耐盐碱品种8份、耐盐碱品种11份、中度耐盐碱品种62份、敏感盐碱品种19份。

关键词：谷子盐碱胁迫萌发期品种筛选

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Postulation of Seedlings Resistance Genes to Yellow Rust in Commercial Wheat Cultivars from Yunnan Province in China

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Agricultural Sciences in China 2011年第11期10卷 1723-1731页

作者： LI Ming-ju FENG Jing CAo shi-qin LIN Rui-ming CHENG Geng CHEN Wan-quan xu shi-chang State Key Laboratory for Biology of Plant Diseases and Insect Pests Institute of Plant Protection Chinese Academy of AgriculturalSciences Beijing 100193 P.R.China Institute of Environment and Resources Yunnan Academy of Agricultural Sciences Kunming 650205 P.R.China Institute of Plant Protection Gansu Academy of Agricultural Sciences Lanzhou 730070 P.R.China Institute of Food Crops Yunnan Academy of Agricultural Sciences Kunming 650205 P.R.China

The objective of this study was to characterize yellow （stripe） rust resistance gene（s） in 52 commercial wheat cultivars from Yunnan Province in China, and to provide information for their rational deployment in field. Seedlings of wheat cultivars were inoculated with 25 differential isolates ofPuccinia striiformis from foreign and home to postulate resistance genes to yellow rust, and then validated by pedigree. There were 10 probable resistance genes characterized in these cultivars, in which, Yr9 was most commonly postulated to be present in thirteen cultivars. Yr21, the second, was present in four cultivars. Yr8, the third, were present in three cultivars. Yr6, Yrl 7 and Yr26, the fourth, was present in two cultivars respectively. The other gene（s） such as, Yr2＋YrA, Yr7 and Yr27, were only present in single cultivar（s）; unknown gene（s） or gene（s） combination（s） were present in 22 cultivars. One cultivar （Yunmai 42） had no resistance gene tested in this study. Cultivars such as Yunmai 52, Mian 1971-98, Kunmai 4, and Yunmai 56 carried effective genes and can be popularized mainly; Yr9 should be planted with other Yr genes. In the meantime other effective genes should be introduced to realize gene diversity for controlling wheat yellow rust. Yunmai 42 should be reduced to avoid rust breakout. Unknown gene cultivars should be utilized and be researched deeply.

关键词： wheat cultivars yellow (stripe) rust resistance genes gene postulation

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Adsorption of Sm on C_(60) Film

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Chinese Physics Letters 1996年第1期13卷 65-68页

作者： PAN Hai-bin xu shi-hong xu chang-shan LU Er-dong XIA An-dong ZHANG Guo-shang xu Peng-shou ZHANG Xin-yi changchun Institute of Physics Academia SinicaChangchun 130021 National Synchrotron Radiation Laboratory University of Science and Technology of ChinaHefei 230029 Structure Research Laboratory University of Science and Technology of ChinaHefei 230026

Using photoemission spectroscopy,we have studied the adsorption of Sin on C_(60) *** tbe first stage of Sm adsorption,the C_(60) valence band shifts to the high binding energy *** maximum shift is about 0.4 *** the meantime,there appears a new structure at the upper side of the highest occupied molecule orbit(HOMO)band,which comes from the hybrid of the Sm 4f^(5) state and C_(60) HOMO *** Sm coverage increases the Sm electron is in a kind of Sm^(2+) state due to the forming of Sm cluster on the surface.

关键词： surface. film. adsorption

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SARS-CoV-2 in the ocular surface of COVID19 patients

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Eye and Vision 2020年第3期7卷 229-231页

作者： Hua-Tao Xie shi-Yun Jiang Kang-Kang xu Xin Liu Bing xu Lin Wang Ming-chang Zhang Department of Ophthalmology Union HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430022China Department of Clinical Laboratory Research Center for Tissue Engineering and Regenerative MedicineUnion HospitalHuazhong University of Science and TechnologyWuhan 430022China

The 2019 novel coronavirus disease(COVID-19)caused by severe acute respiratory syndrome coronavirus 2(SARSCoV-2)has spread globally,while the routes of transmission of this virus are still *** enrolled 33 patients,without any ocular manifestation,with their ocular surface swabs collected for virus *** was detected strong positive in samples of both eyes from two ***,SARS-CoV-2 may exist in the normal ocular surface of COVID-19 patients,suggesting that this virus might be spread through conjunctival contact.

关键词： SARS-CoV-2 COVID-19 Ocular surface

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钠火压力缓解技术研发及验证

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核科学与工程 2024年第3期44卷 642-646页

作者：常一狄杜海鸥潘霖霖王荣东石文涛徐永兴中国原子能科学研究院北京102413

钠冷快堆一旦发生一回路辅助系统主管道大口径破口的钠泄漏事故,泄漏出来的高温钠会迅速燃烧起来,钠燃烧释放的巨大的热能会使发生事故的钠工艺间的温度和压力瞬间升高。本项目通过对混合钠火事故分析的国内外研究进展进行调研,提出针... 详细信息

钠冷快堆一旦发生一回路辅助系统主管道大口径破口的钠泄漏事故,泄漏出来的高温钠会迅速燃烧起来,钠燃烧释放的巨大的热能会使发生事故的钠工艺间的温度和压力瞬间升高。本项目通过对混合钠火事故分析的国内外研究进展进行调研,提出针对混合钠火的压力缓解设备设计,研究发生钠泄漏事故时形成的瞬间高压可以通过压力缓解措施有效释放到相邻工艺小室,且随释压气流带入相邻小室的钠滴燃烧不会超过该小室建筑物的耐压限值。通过模拟一回路钠工艺间设计基准钠泄漏事故,对钠火压力缓解装置进行性能验证,积累钠冷快堆的钠火安全防护系统运行经验。

关键词：钠冷快堆压力缓解钠火防护混合钠火

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Investigation on the Hydro-forming of SUS304 Stainless Steel Dome

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Chinese Journal of Aeronautics 2006年第B12期19卷 261-264页

作者： xu Yong-chao YUAN shi-jian CHEN Yu ZHOU Li-xin KANG Da-chang School of Materials Science and Engineering Harbin Institute of Technology Harbin 150001China Institute of Metal Research Chinese Academy of Sciences Shenyang 110016 China

The dome hydro-forming was studied theoretically and experimentally. The theoretical analysis shows that the process of dome hydro-forming can be divided into two stages, i.e., firstly forming a hemi-sphere and secondly forming a hemi-toroidal. Theoretically, being larger than those in the outer area of a hemi-toroidal shell, the radial tensile stresses take place in the inner area where fracture is more likely to occur in the forming process. The required hydraulic pressure to form the dome should be determined by the deformation in the outer area, hitting about 12 MPa in theory. The experimental results show that the dome could be produced in one operation by hydro-forming which needs a hydraulic pressure of about 9 MPa. The experimental results prove to agree well with the theoretical analysis. In order to avoid wrinkling and fracture, the closing force acted on the die should be adjusted properly in accordance with the hvdraulic nressure.

关键词： hydro-forming dome SUS304 stainless steel

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U型钢冷弯成形过程的有限元仿真

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汽车科技 2024年第2期 64-69页

作者：寇长海高世阳杨树宝许淑芹昌邑市农业农村局发展服务中心潍坊261300 山东浩信股份有限公司潍坊261300 安徽工业大学机械工程学院马鞍山243032

为了提高U型钢的抗扭性能和承载能力,改善外弯角的曲率厚度分布均匀性,并减少尖点在圆角部位的出现,本文使用软件模拟板带连续冷弯成形过程,以揭示U型钢的应力和应变的分布规律。首先,在传统设计方法的基础上进行了成型工艺参数和轧辊... 详细信息

为了提高U型钢的抗扭性能和承载能力,改善外弯角的曲率厚度分布均匀性,并减少尖点在圆角部位的出现,本文使用软件模拟板带连续冷弯成形过程,以揭示U型钢的应力和应变的分布规律。首先,在传统设计方法的基础上进行了成型工艺参数和轧辊的设计,确定了成型道次和每次弯曲的角度;然后,利用有限元软件***,构建了冷弯成型仿真模型,并进行了仿真计算。仿真结果表明:在冷弯过程中,等效应力及等效应变分布呈现弯角处大,立板处次之,而底板处最小的特点,各道次间应力及应变的大小变化较均匀,成型力分布也比较合理。

关键词：冷弯有限元模拟变形成型力

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Modified Tong Xie Yao Fang relieves solitary rectal ulcer syndrome:A case report

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World Journal of Clinical Cases 2019年第15期7卷 2058-2064页

作者： Li-Li Zhang Wan-Shan Hao Meng xu chang Li Yuan-Yuan shi School of Life Sciences Beijing University of Chinese MedicineBeijing 100029China Teaching and Research Section of Shanghan Beijing University of Chinese MedicineBeijing 100029China Traditional Chinese Medicine Department Beijing Baicaoyuan Hospital of Traditional Chinese MedicineBeijing 100107China

BACKGROUND Solitary rectal ulcer syndrome(SRUS)is a rare rectal disorder characterized by bloody mucus in the stool,difficulty in defecation,pain,and anal *** date,the etiology of this syndrome remains not well understood and the diagnosis is frequently confused with other disorders,making treatment a clinical *** SUMMARY A 50-year-old woman presented to our hospital with a 40-d history of bloody mucus in the stool and anal *** was *** revealed a large,severe ulcerous ***,the lesion was characterized as chronic ulcer without clear tumor cells,and the final diagnosis of SRUS was *** patient was treated with Chinese medicine therapy,with administration of Tong Xie Yao *** 3 wk of treatment,the symptoms improved *** 2-mo follow-up,rectoscopy in a local hospital showed healed ulcer scars without obvious protrusion 3 cm from the anal *** Chinese medicine therapy represents a potential treatment of SRUS with predominant rectal bleeding,mucinous discharge,and anal swelling pain.

关键词： Solitary rectal ulcer syndrome Chinese formulas Tong Xie Yao Fang Er Shen Wan Ding Zhi Xiao Wan Chinese medicine therapy Case report

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