检索结果-南通市图书馆

华东六省一市生物化学与分子生物学会2003年学术交流会

作者： Liu Zhigang,Yang Hui,Su Dongming,Luo Shiwen (College of Life Sciences, shenzhen University.shenzhen 518060 shenzhen key laboratory of microbial genetic engineering.shenzhen 518060)

Background: Inhalation of pollen in the atmosphere provokes IgE-mediated responses of hay fever and allergicasthma in about 20% of humans. Artemisia is the dominant source of allergens in china because of its prodigiousproduction of airborne pollen. There are many species of Artemistia around the world, but Artemisia apiacea is mostimportant. Purification and identification of major allergens of them have not been reported so far. Objective: To i-dentify and purify the major allergens from the A. apiacea pollen crude extracts. Methods: A. apiacea was extrac-ted in the coca’s solution after being defatted. Proteins were then concentrated by means of saturated ammoniumsulfate homogenous precipitation. Western-blotting was done to identify allergens using sera from subjects allergie toArtemisia pollen. DEAE-Cellulose DE-32 Ion Exchange chromatography(IEC) was used for primary purification.

关键词：

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

Refolding, purification and characterization of the major cockroach Cr PI allergen from Escherichia coli inclusion bodies

Refolding, purification and characterization of the major co...

引用

华东六省一市生物化学与分子生物学会2003年学术交流会

作者： Gao Bo,Liu Zhigang ,Hong Shiyi, Luo Shiwen (College of Life Sciences, shenzhen University.shenzhen,518060 shenzhen key laboratory of microbial genetic engineering.shenzhen 518060

Airborne cockroach allergens play an important role in allergic diseases, especially asthma. A lambda ExcellcDNA library was constructed from Periplaneta Americana nymph mRNA. The gene encoding Cr PI, the major al-lergen of P. America nymph was isolated with sera from subjects allergic to cockroach and subcloned into the fusionexpression vector of pGEX-5X-1 (Pharmacia), resulting in a GST-Cr P1 fusion gene construction (pGEX-5X-1/CrP1). This plasmid was transformed into and expressed by the Escherichia coli strain BL21. Most of the expressedfusion protein was found in the insoluble fraction (inclusion body) of the cell lysate.. After intensive washing, the

关键词：

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

Construction and primary characterization of cDNA expression library of Artemisia apiacea pollen

Construction and primary characterization of cDNA expression...

引用

华东六省一市生物化学与分子生物学会2003年学术交流会

作者： Liu Zhigang, Gao Bo, He YiHua,Su Dongming, Zhong suping, Wu xiaoman College of Life Sciences, shenzhen University.shenzhen,518060 shenzhen key laboratory of microbial genetic engineering.shenzhen, 518060 Second Affiliated Hospital of Guangzhou Medical College, Guangzhou,510260)

Backgrouds:Allergic diseases including extrinsic asthma(allergic asthma),allergic rhinitis *** defined as a

关键词：

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

Expression, Characterization and Purification of the Major Cockroach Cr PI Allergen

Expression, Characterization and Purification of the Major C...

引用

中国蛋白质组学首届学术大会

作者： Gao Bo, Liu Zhigang,Hong Shiyi, Luo Shiwen (College of Life Sciences, shenzhen University,shenzhen 518060 shenzhen key laboratory of microbial genetic engineering, shenzhen 518060)

Backgroud: Airborne cockroach allergens play an important role in allergic diseases, especially *** and therapy of cockroach allergy would benefit from the availability of defined recombinant ***: The purpose of this study was to express and purify recombinant Cr PI, a major allergen of ***: A lambda Excell cDNA expression library was constructed from Periplaneta Americana nymph *** sera from subjects allergic to cockroach,the cDNA library was immunoscreened and the isolated Cr PIgene was then subcloned into the fusion expression vector of pGEX-5X- 1 (Pharmacia).By means of WesternBlotting, the IgE-binding ability of the recombinant allergen was evaluated. Most of the expressed fusion pro-tein was found in the insoluble fraction (inclusion body) of the cell lysate. After intensive washing, the proteins

关键词：

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

A PSTAIRE CDK-like protein localizes in nuclei and cytoplasm of Physarum polycephalum and functions in the mitosis

引用

Cell Research 2004年第2期14卷 169-175页

作者： Gui Ying LI~(1,2), Miao XING~(1,*), Bo HU~(1,3) ~1College of Life Science, shenzhen key laboratory of microbial Gene engineering, shenzhen University, shenzhen 518060, China. ~2Department of Immunology, School of Basic Medical Sciences, Jilin University, Changchun 130021, China ~3Institute of genetics and Cytology, Northeast Normal University, Changchun 130024, China. College of Life Science Shenzhen Key Laboratory of Microbial Gene Engineering Shenzhen University Shenzhen 518060 China.

CDKs play key roles in controlling cell cycle progression in all eukaryotes. In plants, multiple CDKs are present, among which the best characterized CDKs are PSTAIRE CDKs. In this study, we carried out Western blot, immunoelectron microscopy and antibody treatment with an anti-PSTAIRE monoclonal antibody to explore the subcellular localization and functions of PSTAIRE CDKs in Physarum polycephalum. The results of Western blot and immunoelectron microscopy showed that in P. polycephalum, a PSTAIRE CDK-like protein was 34 kD in molecular weight and located in both nuclei and cytoplasm. In nuclei, the protein was mainly associated with chromosomes and nucleoli. The expression of the PSTAIRE CDK-like protein in both the plasmodia and nuclei showed little fluctuation through the whole cell cycle. When treated with an anti-PSTAIRE monoclonal antibody at early S phase, the cells were arrested in S phase, and the mitotic onset of P. polycephalum was blocked for about 1 h when treated at early G2 phase. Our dat

关键词： PSTAIRE CDK-like protein Physarum polycephalum mitosis immunoelectron microscopy.

来源：

同方期刊数据库评论

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Genomic research for important pathogenic bacteria in China

引用

Science China(Life Sciences) 2009年第1期52卷 50-63页

作者： YANG RuiFu1,GUO XiaoKui2,YANG Jian3,JIANG YongQiang1,PANG Bo4,CHEN Chen5,YAO YuFeng2,QIN JinHong2 & LI QingTian2 1 laboratory of Analytical Microbiology,State key laboratory of Pathogen and Biosecurity,Beijing Institute of Microbiology and Epidemiology,Beijing 100071,China 2 Department of Medical Microbiology and Parasitology,Institutes of Medical Sciences,Shanghai Jiao Tong University School of Medicine,Shanghai 200025,China 3 State key laboratory for Molecular Virology and genetic engineering,Institute of Pathogen Biology,Chinese Academy of Medical Sciences,Beijing 100176,China 4 State key laboratory for Infectious Disease Prevention and Control,Institute for Communicable Disease Control and Prevention,Chinese Center for Disease Control and Prevention,Beijing 102206,China 5 Beijing Genomics Institute at shenzhen,shenzhen 518083,China laboratory of Analytical Microbiology State Key Laboratory of Pathogen and Biosecurity Beijing Institute of Microbiology and Epidemiology Beijing 100071 Chin

Rapid accumulation of bacterial genomic data offered an unprecedented opportunity to understand bacterial biology from a holistic view of *** can thus closely look at the way in which a pathogen is evolved,and these data has been applied to molecular epidemiology and microbial forensics,and screening of novel diagnostic,vaccine and drug *** newly developed high-throughput low-cost sequencing technologies,such as 454,Solexa and SOLiD,will promote the acquisition and application of genomic data in new research areas that we dared not imagine previously,such as the metagenomics of human gastric-intestinal tract,for better and comprehensive understanding of human health and disease.

关键词： genome pathogenic bacteria sequencing application

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Cloning and expression of Haematococcus pluvialis fatty acid synthsis genes and its relationship with fatty acid accumulation

Cloning and expression of Haematococcus pluvialis fatty acid...

引用

中国藻类学会第八次会员大会暨第十六次学术讨论会

作者： An-ping Lei~1,Huan Chen~1,Chao-gang Wang~1,Zhang-li Hu and Jiang-xin Wang~(2,*) 1 shenzhen key laboratory for Marine Bio-resource and Eco-environment,College of Life Science,shenzhen University,shenzhen 518060,P.R.China 2 Biodesign Institute,Arizona State University,Tempe,AZ 85287,USA

＜正＞Biofuel has been an incredibly hot research topic recently in both academia and *** feedstocks include crops like corn,soybeans,flaxseed rapeseed/canola,and microorganisms,such as algae,yeast,bacteria,and *** them,microalgae become more attractive due to their rapid growth,high oil content, high density culture and with no competition with food resources and agricultural land. To blast through the bottleneck of high biofuel production cost from microalgae, synthesis biology techniques are highly required to improve content of algal fatty acids in *** fatty acid biosynthesis pathway genes have been all cloned and biosynthesis pathway was built up in some higher plants and algae,the molecular mechanism for its regulation is far away from *** this end,we cloned main key genes for fatty acid biosynthesis in Haematococcus pluvialis,a green alga with high commercial values,and investigated relationships between their expression alternation and fatty acid profiling detected by GOMS under different stress treatments, *** depletion,salinity,high or low *** results showed that high temperature,high salinity,and nitrogen depletion treatments played significant role in promoting algal fatty acid *** analysis showed that acyl carrier protein（ACP）,3-ketoacyl-ACP-synthase（KAS）,and acyl-ACP thioesterase（FATA） gene expression had significant or highly significant correlations with monounsaturated fatty acid synthesis（positive） and polyunsaturated fatty acid synthesis（negative）. We proposed that ACP,KAS and FATA in Haematococcus play an important role in fatty acid synthesis and may be rate limiting genes,which could be modified for the further study of genetic engineering,metabolic engineering to improve algae biofuel quality and production.

关键词： Haematococcus pluvialis fatty acid synthesis fatty acid synthase gene gene expression

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

大豆LEA蛋白Em的表达可提高大肠杆菌和烟草耐盐性

引用

深圳大学学报（理工版） 2006年第3期23卷 230-236页

作者：蔡丹郑易之兰英深圳大学生命科学学院深圳市微生物基因工程重点实验室深圳518060

将大豆Em(LEA1)基因构建到原核表达载体pET28-Em中.转化大肠杆菌,经SDS-PAGE电泳及电喷雾质谱鉴定.结果显示,经IPTG诱导的重组菌可表达约18kDa的特异蛋白.这种蛋白具有良好的可溶性和热稳定性.在含800 m mol/L NaC l培养基中,含空载体... 详细信息

将大豆Em(LEA1)基因构建到原核表达载体pET28-Em中.转化大肠杆菌,经SDS-PAGE电泳及电喷雾质谱鉴定.结果显示,经IPTG诱导的重组菌可表达约18kDa的特异蛋白.这种蛋白具有良好的可溶性和热稳定性.在含800 m mol/L NaC l培养基中,含空载体的对照菌生长迟滞期约为50 h,含Em基因的重组菌生长迟滞期为32 h,表明大豆Em蛋白的表达可提高大肠杆菌对盐胁迫的适应能力及耐盐性.在此基础上,构建了含Em基因的真核表达载体pB I-Em.再利用根癌农杆菌介导法将该基因转入烟草.PCR及RT-PCR结果显示,大豆Em基因已整合到转基因烟草的基因组DNA中,并能正常转录.转化率为53%.转Em基因烟草植株在含有质量分数1.5%NaC l的培养基中生长状况好于对照植株,叶片的叶绿素含量高于对照植株的叶片.结果表明,大豆Em基因的表达可提高大肠杆菌和转基因烟草的耐盐性.

关键词：大豆 LEA1基因 Em蛋白大肠杆菌根癌农杆菌转化转基因烟草盐胁迫

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：