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Effectiveness of Testosterone Undecanoate Treatment in Men with Asthenospermia

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Journal of Reproduction and Contraception 2012年第2期23卷 119-126页

作者： Bo SONG Zhi-ming CAI Guangdong Key Lab Male Reproductive Medicine&GeneticsShenzhen HospitalPeking UniversityShenzhen 518036China

Objective To assess the effectiveness of testosterone undecanoate on sperm motility and pregnancy incidence in men with asthenospermia. Methods A clinical trial was performed. Fifty men with asthenospermia were included to receive placebo （control group, n=9） or T undecanoate 80 mg/d （study group, n=41）. Pregnancy incidence and sperm characteristics after 1, 2 and 3 months of medication and 3 months after the end of the trial were measured. Results Compared with the placebo, T undecanoate treatment produced a satisfactory improvement of seminal motility （F=55.904, P=0.000）. In study group, the incidence of pregnancy was 28.2% while the incidence of pregnancy in control group was 11.1%. In study group, 26patients took T undecanoate for more than 3 months. In the 3 months, semen volume showed no statistical difference （F=1.206, P=0.312） before and after treatment, sperm concentration （F=0.023, P=0.000） and motility a, b, a ＋b （P=0.000） showed statistical differences. Motility grade a showed significantly higher increment in 2 and 3 months after treatment than 1 month and there was no statistical difference between 2 and 3 months. So did grade b. Conclusion The results indicate that T undecanoate increases semjnal motility, leading to a higher incidence of pregnancy in couples with infertility related to asthenoaspermia.

关键词： testosterone undecanoate sperm motility asthenospermia

Effectiveness of Combined Testosterone Undecanoate with Tamoxifen Citrate Treatment in Men with Idiopathic Azoospermia or Serious Oligozoospermia

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Journal of Reproduction and Contraception 2012年第4期23卷 254-258页

作者： Bo SONG Wei-ping QIAN Guangdong Key Lab. Male Reproductive Medicine & Genetics Reproductive Medicine Department Shenzhen Hospital Peking University

Objective To assess the effect of treatment with a combination of the tamoxifen citrate and testosterone undecanoate on sperm variables in men with idiopathic azoospermia or oIigozoospermia. Methods Four men with idiopathic azoospermia and 8 men with idiopathic oligozoospermia were collected in this retrospective and self control trial. All patients were treated with testosterone undecanoate of 80 mg/d and tamoxifen citrate of 20 mg/d. Sperm characteristics and pregnancy incidence were measured every month during 4 months medication. Results Tamoxifen citrate plus testosterone undecanoate treatment produced a satisfactory improvement of total sperm number, motility after 2 and 3 months whereas serum FSH, LH levels increased after 2 or 3 months. Conclusion The combination of tamoxifen citrate with testosterone undecanoate could improve significantly sperm count. The combination could be used in men with idiopathic azoospermia or serious oligozoospermia.

关键词： testosterone tamoxifen citrate oligozoospermia azoospermia

The Comparison Study of Renin and Angiotensin AⅡ Levels on Normal Tension Glaucoma Patients and Normal Individuals

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眼科学报 2005年第3期 64-67页

作者： Hongmin Yun, Pei Fu, Kexi Ding, Qi Yang Department of Ophthalmology, Peking University shenzhen Hospital, shenzhen 518036, China Department of Ophthalmology Peking University Shenzhen Hospital Shenzhen 518036 China

Purpose: To investigate the levels of renin-angiotension system (RAS) components in normal tension glaucoma patients and normal controls. Methods: Blood samples were obtained from 11 normal tension glaucoma(NTG)patients and 11 age and sex matched controls. The levels of renin and angiotensin AⅡof 11 NTG patients and normal controls were examined by radio-immunity test. Statistical analyses were performed by paired t test. Results:The levels of renin of NTG patients and normal controls are (769.085±183.217) pg/ml/n and (822.035 ±124.140) pg/ml/n, while the levels of angiotensin A Ⅱof NTG patients and normal controls are (37.347±10.669)pg/ml and (24.836±10.665)pg/ml respectively. No statistically significant differences were observed between the levels of renin and angiotensin among NTG patients and normal controls. Conclusion: There were not many abnormalities of the levels of circulating rennin and angiotensin AⅡof NTG patients in our study. Eye Science 2005 ;21 :192-195.

关键词： normal tension glaucoma renin angiotensin AⅡ renin-angiotension system

Investigation on Azoospermia Factor (AZF) Microdeletion and Sex-determining Region Y (SRY) of the Y Chromosome in Male Infertility

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Journal of Reproduction and Contraception 2013年第2期24卷 88-94页

作者： Li-hua LU Fei-xue HU Song GE Laboratory of Genetics Deptment of Laboratory Medicine Peking University Shenzhen Hospital

Objective To determine the incidence of azoospermia faetor （AZF） microdeletions of Y chromosome in male infertility and to investigate the mechanism of sex-determining region Y （SRY） in sex differentiation. Methods The mierodeletion of AZF was detected by multiplex polymerase chain reaction （PCR） using Y-chromosome specific sequence tagged sites （STSs）, and SRY was analyzed by PCR and sequencing. Results There were 100 cases with AZF microdeletion and the ratio of AZF microdeletion was 6.8% over all 1 474 cases. The ratios of AZF microdeletion of azoospermia group and severe oligozoospermia group were 9.0% and 7.1%, respectively, which was significantly different from oligozoospermia group （P〈 0. 05）. There were 67 cases with 5 STSs mierodeletion of sY152, sY239, sY243, sY254 and sY255. There were 20 cases with long fragment deletion more than 10 STSs, and the patterns of AZF microdeletion in other 13 cases were rare. In all 9 patients with disorders of sex differentiation, there were 6patients with SRY-absent and AZF-absent. There was no mutation of SRY gene by sequencing in other 3 patients with SRY-positive. Conclusion Deletions in AZF region of Y chromosome are specific with diagnoses with spermatogenesis disorder. Deletions of sY152, sY239, sY243, sY254 and sY255 occur the most frequently. SRY was an important candidate gene of testis-determining factor （TDF） gene.

关键词： sex-determining region Y (SRY) azoospermia factor (AZF) sex differentiation spermatogenesis

Different Pretreatments before Application of GnRH Antagonist Protocol in Controlled Ovarian Hyperstimulation

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Journal of Reproduction and Contraception 2011年第4期22卷 201-210页

作者： Ping-xiu HUANG Rong LI Min FU Juan-juan WANG Reproductive Medical Centre Shenzhen Hospital of Peking University Shenzhen 518036 China

Objective To analyze the clinical features and outcome of patients who used different pretreatments before application of gonadotropin-releasing hormone antagonist protocol during in vitro fertilization - embryo transfer （IVF-ET） cycles, and to explore how effective to use the antagonist protocol. Methods A retrospective analysis was performed. All the ET cycles were divided into three groups, group A （n=125） used short acting GnRH agonist before GnRH antagonist treatment, group B （n=113） used short-acting oral contraceptives before GnRH antagonist treatment, group C （n=81） was untreated before GnRH antagonist treatment. All the patients had no tubal fluid, endometrial polyps and no anatomical abnormalities of the uterus, from April 2010 to December 2010. The patient＇s age, dose and duration of gonadotropin （Gn） treatment, the serum LH and E2 levels on the day of hCG injection, the number of oocytes retrieved, the rates of good-quality embryos, the clinical pregnancy rates were compared. At the same time, 261 GnRH agonist long protocol cycles （group D） were selected at the same period as further comparison. Results The patients in group C （32.9 ~ 4.8 years） were significantly older than those in groups A and B （31.6 ___＋3.7 years, 31.2 ___%4.1 years）（P 〈0.05）. The dose and the duration of Gn in group C were significantly lower than those in groups A and B. The serum LH level on the day of hCG injection in group A and group B was significantly lower than that in group C （P 〈0.05）, especially in group A. The endometrium was the thinnest in group B. There were no significant differences in the fertilization rates and the good-quality embryosrates among them. The clinical pregnancy rate of group B decreased significantly compared with groups A and C （P〈0. 05）. The clinical pregnancy rate of group C was the highest among them. There was no significant difference of clinical pregnancy rates between group C and group D （37% vs 40.2%,P〉0.05）. However, the dose （19.8 ±6.6 am

关键词： in vitro fertilization-embryo transfer (IVF-ET) gonadotropin-releasing hormone antagonist short-acting oral contraceptives short-acting agonist

Induction of bone marrow stromal cells into neuron-like cells by vascular endothelial growth factor

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Induction of bone marrow stromal cells into neuron-like cell...

Proteomic analysis of nuclear matrix proteins during arsenic trioxide induced apoptosis in leukemia K562 cells

中国神经科学学会第四次会员代表大会暨第八届全国学术会议

作者： Qiao-Yun LIU Li YI Jin-Ling HAN Ye jING Fang-Ting ZHANG Guang-Hui CUI 1 Medical College of Shantou University Shantou 515041 China. 2 Department of Neurology Peking University shenzhen Hospital shenzhen 518036 China 3 Central Laboratory Peking University shenzhen Hospital shenzhen 518036 China 4 Laboratory of Male Reproductive Medicine Peking University shenzhen Hospital shenzhen 518036 China

＜正＞Objective To investigate the possibility of induction of bone marrow stromal cells（BMSCs） into neuron-like cells by vascular endothelial growth factor（VEGF） in vitro,so as to provide experimental basis for the treatment of brain injury by cell transplantation and *** Bone marrow stromal cells were harvested from young Sprague-Dawley rats and cultured in DMEM/F12 suppplemented with 10%fetal bovine *** BMSCs were passaged 3 times and characterized by flow *** BMSCs were pre-induced by basic fibroblast growth factor（b-FGF, 10ng/ml） for 24h,then they were treated with different concentrations of vascular endothelial growth factor（VEGF）,which was used to induce BMSCs into neuron-like *** experiment was separated into four groups:Group A（contains 5ng/ mlVEGF）,Group B（contains 10ng/miVEGF）,Group C（contains 20ng/mlVEGF） and Group D served as a control group never adding *** contrast microscope was used to observe the differentiation of BMSCs daily,and the total number of neuron-like cells in 20 random fields（×200） was count at 3 and 10 days after *** technique was used to identify the differentiated BMSCs with neuron specific enolase（NSE）.Result The cultured BMSCs were CD90 positive and CD45 negative on flow *** 3 days after induction,some BMSCs in groups A～C were observed to change the shapes:cell bodies appeared refractile,cone-like,or spherical with primary and secondary *** 10 days after induction,the number of the cells which assumed neuronal morphology increased in groups A～*** in morphology of a few of BMSCs in group D was *** were the most neuron-like cells in group B,P＜0.05 for group B versus other *** were the fewest neuron-like cells in group D,P＜0.05 for group D versus other *** the neuron-like cells at 10 days after induction was more than those at 3 days after induction in all the groups,P＜0.05 for the numbers of the neuron-like ce

关键词： vascular endothelial growth factor bone marrow stromal cells neuron-like cells cell differentiation

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中华医学杂志(英文版) 2005年第2期000卷 14-18页

作者： WANG Zi hui, YU Ding, CHEN Yan and HAO Jian zhong Department of Pathology and Department of Surgery , Beijing University, shenzhen Hospital, shenzhen 518036, China Department of Hematology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China Department of Pathology Beijing University Shenzhen Hospital Shenzhen 518036 China Department of Surgery Beijing University Shenzhen Hospital Shenzhen 518036 China Department of Hematology Union Hospital Tongji Medical College Huazhong University of Science and Technology Wuhan 430022 China

Background Arsenic trioxide (As2O3) has been identified as a very potent antiacute leukemic agent However its role in apoptosis needs to be elucidated As2O3 interferes with the proliferation and survival of tumor cells via a variety of mechanisms Drugtarget interactions at the level of nuclear matrix (NM) may be critical events in the induction of cell death by As2O3 This study dealt with As2O3-target interactions at the level of NM in chronic myelogenous leukemia cell line K562 by proteomics Methods K562 cells were cultured in MEM and treated with different concentrations of As2O3 The nuclear matrix proteins were analyzed by highresolution twodimensional gel electrophoresis and computerassisted image analysis Results As2O3 significantly inhibited the growth of chronic myelogenous leukemia cell line K562 at low concentrations While more than 200 protein spots were shared among the nuclear matrices, about 18 distinct spots in the nuclear matrices were found characteristic for As2O3 treated cells Conclusions: As2O3 induces apoptosis in K562 cells in a dose and timedependent manner Our results demonstrated that for the detection of the onset of apoptosis, the alteration in the composition of nuclear matrix proteins was a more sensitive indicator than nucleosomal DNA fragmentation test These results indicated that As2O3 might be clinically useful in the treatment of chronic myelogenous leukemia. The changes of nuclear matrix proteins in the treated cells can be used as a useful indicator for this treatment

关键词： Arsenic trioxide · nuclear matrix · chronic myelogenous leukemia · K562 cells · apoptosis · proteomics

Genes Associated Germ Cells from Mouse Embryonic Stem on Exogenous Extracellular Matrix

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Genes Associated Germ Cells from Mouse Embryonic Stem on Exo...

Expression and Bioinformatics Analysis of SPACA4 in Human and Mice

2008年全国生物化学与分子生物学学术大会

作者： Zheng-yu Qi Xin Guo Jie Qin Yang-ting Gui Zhi-ming Cai (The Key Laboratory of Male Reproductive and Genetic,shenzhen Hospital of Peking University,shenzhen 518036,China.)

Extracellular matrix (ECM) influence stem-cell behavior,although the exact effects elicited in embryonic stem (ES) cells are poorly *** previously developed a culture medium that contains leukemia inhibi- tory factor ...

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Journal of Reproduction and Contraception 2008年第1期19卷 9-15页

作者： Ai-fa TANG Zhen-dong YU Yao-ting GUI Xin GUO Xian-xin LI Wei-xiang LIU Hui ZHU Zhi-ming CAI shenzhen Key Lab of Male Reproduction and Genetics Shenzhen Hospital of Peking University Shenzhen 518036 China Research Center of Biomedical Engineering Life Science Division Graduate School at Shenzhen Tsinghua University Shenzhen 518055 China

Objective To analyze the expression of SPACA4 in human and mice. Methods Testes cRNA samples from Balb/c mice of different postnatal days were performed with mouse affymetrix chip to screen the expression of SPACA4 in mice. Sub-quantitative RT-PCR and bioinformatic tools were used here to describe the expression profile of SPACA4 in mice and human. Results The results of gene chip analysis indicated that the expression of mSPACA4 began after d 35 of postnatal testis in mice. Sub-quantitative RT-PCR assay showed that SPACA4 gene expressed exclusively in mouse and human testis, and mouse mSPACA4 gene expressed after d 35 of postnatal testis that was consistency with the results of gene chip analysis. By bioinformatics analysis, mSPACA4 is located in cell membrane （34.8%） or plasma membrane （34.8%）, the signal peptide cleavage site between position 19 and 20 amino acids, transmembrane region between 2-20 and 101-126 amino acids, respectively, on mSPACA4 protein. Conclusion mSPACA4 and hSPACA4 were testis-specific genes, and the expression of mSPACA4 begins after d 35 of postnatal testis in mice. SPACA4 is a candidate for targeting in a sperm-based contraceptive vaccine.

关键词： gene chips SPACA4 gene spermatogenesis male contraceptive vaccine