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Nuclear PLD1 combined with NPM1 induces gemcitabine resistance through tumorigenic IL7R in pancreatic adenocarcinoma

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癌症生物学与医学:英文版 2023年第8期20卷 599-626页

作者： Danqi Fu Jingrui Yan Zhaoyu Zhang Yang liu Xiaoqing Ma Jinsheng Ding shengyu Yang Ran Zhao Antao Chang Chuntao Gao Jing liu Tiansuo Zhao Xiuchao Wang Chongbiao Huang Song Gao Ying Ma Bo Tang Yukuan Feng Hongwei Wang Jihui Hao Department of Pancreatic Cancer Tianjin Medical University Cancer Institute and HospitalNational Clinical Research Centre for CancerKey Laboratory of Cancer Prevention and TherapyTianjin 300060China Department of General Surgery Changzheng HospitalNaval Medical University(Second Military Medical University)Shanghai 200003China Department of Cellular and Molecular Physiology Penn State College of MedicineHersheyPennsylvania 17033USA

Objective:Pancreatic ductal adenocarcinoma(PDAC)is a highly malignant gastrointestinal cancer with a 5-year survival rate of only 9%.Of PDAC patients,15%-20%are eligible for radical surgery.Gemcitabine is an important chemotherapeutic agent for patients with PDAC;however,the efficacy of gemcitabine is limited due to resistance.Therefore,reducing gemcitabine resistance is essential for improving survival of patients with PDAC.Identifying the key target that determines gemcitabine resistance in PDAC and reversing gemcitabine resistance using target inhibitors in combination with gemcitabine are crucial steps in the quest to improve survival prognosis in patients with PDAC.Methods:We constructed a human genome-wide CRISPRa/dCas 9 overexpression library in PDAC cell lines to screen key targets of drug resistance based on sgRNA abundance and enrichment.Then,co-IP,ChIP,ChIP-seq,transcriptome sequencing,and qPCR were used to determine the specific mechanism by which phospholipase D1(PLD1)confers resistance to gemcitabine.Results:PLD1 combines with nucleophosmin 1(NPM1)and triggers NPM1 nuclear translocation,where NPM1 acts as a transcription factor to upregulate interleukin 7 receptor(IL7R)expression.Upon interleukin 7(IL-7)binding,IL7R activates the JAK1/STAT5 signaling pathway to increase the expression of the anti-apoptotic protein,BCL-2,and induce gemcitabine resistance.The PLD1 inhibitor,Vu0155069,targets PLD1 to induce apoptosis in gemcitabine-resistant PDAC cells.Conclusions:PLD1 is an enzyme that has a critical role in PDAC-associated gemcitabine resistance through a non-enzymatic interaction with NPM1,further promoting the downstream JAK1/STAT5/Bcl-2 pathway.Inhibiting any of the participants of this pathway can increase gemcitabine sensitivity.

关键词： Gemcitabine resistance pancreatic ductal adenocarcinoma phospholipase D1 nucleophosmin 1 CRISPRa library

Preparation of lignin/TiO2 nanocomposites and their application in aqueous polyurethane coatings

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Frontiers of Chemical Science and Engineering 2019年第1期13卷 59-69,F0004页

作者： Dongjie Yang shengyu Wang Ruisheng Zhong Weifeng liu Xueqing Qiu School of Chemistry and Chemical Engineering South China University of Technology Guangzhou 510640 China State Key Laboratory of Pulp and Paper Engineering South China University of Technology Guangzhou 510640 China

A simple method using a water soluble lignin quaternary ammonium salt (LQAS) and TiO2 has been developed for the preparation of lignin/TiO2 nanocomposites in an aqueous medium under mild conditions. The LQAS/TiO2 nanocomposites contain well-dispersed small particles with excellent ultraviolet (UV) shielding abilities and good compatibilities with waterborne polyurethane (WPU). When the LQAS/TiO2 nanocomposites were blended with WPU, the UV absorbance and the tensile ductility of the WPU increased significantly. The composite WPU hybrid film containing 6 wt-% LQAS/TiO2 nanocomposite had the highest visible light transmittance and had excellent ultraviolet aging properties. After 192 h of UV light irradiation, the tensile strength of the composite film was above 8 MPa and the elongation at break was 800%. This work highlights new possibilities for the utilization of alkali lignin.

关键词： lignin TiO2 nanocomposite particle polyurethane film

Microstructure, mechanical and tribological properties of TiAl-based composites reinforced with high volume fraction of nearly network Ti_2AlC particulates

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Journal of Materials Science & Technology 2018年第4期34卷 670-678页

作者： Jun Cheng shengyu Zhu Yuan Yu Jun Yang Weimin liu State Key Laboratory of Solid Lubrication Lanzhou Institute of Chemical PhysicsChinese Academy of Sciencestanzhou 730000China

TiAl-based composites reinforced with different high volume fractions of nearly network TizAIC phase have been successfully prepared by mechanical alloying and hot-pressing method. Their microstructure. mechanical and tribological properties have been investigated. Ti2AIC network becomes continuous but the network wall grows thicker with increasing the Ti2AIC content. The continuity and wall size of the network Ti2AIC phase exert a significant influence on the mechanical properties. The bending strength of the composites first increases and then decreases with the Ti2A1C content. The compressive strength of the composite decreases slightly compared to the TiAI alloy, but the hardness is enhanced. Due to the high hardness and load-carrying capacity of the network structure, these composites have the better wear resistance. And this enhancement is more notable at low applied loads and high Ti2A1C content. The mechanisms simulating the role of network Ti2AIC phase on the wear behavior and the wear process of TiAl/Ti2AIC composites at different applied loads have been proposed.

关键词： TiAl/Ti2AIC composites Network structure Wear resistance

Dietary fiber during gestation improves lactational feed intake of sows by modulating gut microbiota

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畜牧与生物技术杂志:英文版 2023年第5期14卷 2012-2032页

作者： Shuang Li Jie Zheng Jiaqi He Hao liu Yingyan Huang Liansu Huang Ke Wang Xilun Zhao Bin Feng Lianqiang Che Zhengfeng Fang Jian Li shengyu Xu Yan Lin Xuemei Jiang Lun Hua Yong Zhuo De Wu Key Laboratory for Animal Disease‑Resistance Nutrition of China Ministry of Education Institute of Animal NutritionSichuan Agricultural UniversityNo.211Huimin RoadWenjiang DistrictChengdu 611130SichuanPeople’s Republic of China

Background The feed intake of sows during lactation is often lower than their needs.High-fiber feed is usually used during gestation to increase the voluntary feed intake of sows during lactation.However,the mechanism underlying the effect of bulky diets on the appetites of sows during lactation have not been fully clarified.The current study was conducted to determine whether a high-fiber diet during gestation improves lactational feed intake(LFI)of sows by modulating gut microbiota.Methods We selected an appropriate high-fiber diet during gestation and utilized the fecal microbial transplanta-tion(FMT)method to conduct research on the role of the gut microbiota in feed intake regulation of sows during lactation,as follows:high-fiber(HF)diet during gestation(n=23),low-fiber(LF)diet during gestation(n=23),and low-fiber diet+HF-FMT(LFM)during gestation(n=23).Results Compared with the LF,sows in the HF and LFM groups had a higher LFI,while the sows also had higher peptide tyrosine tyrosine and glucagon-like peptide 1 on d 110 of gestation(G110 d).The litter weight gain of piglets during lactation and weaning weight of piglets from LFM group were higher than LF group.Sows given a HF diet had lower Proteobacteria,especially Escherichia-Shigella,on G110 d and higher Lactobacillus,especially Lactobacil-lus_mucosae_LM1 and Lactobacillus_amylovorus,on d 7 of lactation(L7 d).The abundance of Escherichia-Shigella was reduced by HF-FMT in numerically compared with the LF.In addition,HF and HF-FMT both decreased the perinatal concentrations of proinflammatory factors,such as endotoxin(ET),lipocalin-2(LCN-2),tumor necrosis factor-α(TNF-α),and interleukin-1β(IL-1β).The concentration of ET and LCN-2 and the abundance of Proteobacteria and Escheri-chia-Shigella were negatively correlated with the LFI of sows.Conclusion The high abundance of Proteobacteria,especially Escherichia-Shigella of LF sows in late gestation,led to increased endotoxin levels,which result in inflammatory responses and adverse effects on the LFI of sows.Add

关键词： Dietary fiber Escherichia-Shigella Feed intake Lactobacillus Sow

危重烧伤老年患者早期临床特征的多中心回顾分析及预后的危险因素分析

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中华烧伤与创面修复杂志 2024年第3期40卷 249-257页

作者：马琪敏汤文彬李孝建常菲尹希陈昭宏吴国华夏成德李晓亮王德运储志刚张逸王磊吴抽浪童亚林崔培郭光华朱志豪黄圣宇常刘刘锐刘永计王玉松刘晓彬沈拓朱峰海军军医大学第一附属医院烧伤外科上海200433 同济大学附属东方医院重症医学科上海200120 暨南大学附属广州市红十字会医院烧伤整形科广州510220 张家港市第一人民医院烧伤整形科张家港215600 福建医科大学附属协和医院烧伤科福州350001 郑州市第一人民医院烧伤科郑州450004 武汉大学同仁医院暨武汉市第三医院烧伤科武汉430060 南通大学附属医院烧伤整形科南通226001 浙江省台州医院烧伤科临海317000 解放军第924医院烧伤整形科桂林541002 南昌大学第一附属医院烧伤整形与创面修复医学中心南昌330006 大连市第四人民医院烧伤整形科大连116031 黑龙江省医院烧伤科哈尔滨150036

目的探讨危重烧伤老年患者早期临床特征及预后的危险因素。方法该研究为回顾性病例系列研究。收集2015年1月—2020年12月12家医院收治的符合入选标准的124例危重烧伤老年患者的临床资料,其中大连市第四人民医院4例、福建医科大学附属协... 详细信息

目的探讨危重烧伤老年患者早期临床特征及预后的危险因素。方法该研究为回顾性病例系列研究。收集2015年1月—2020年12月12家医院收治的符合入选标准的124例危重烧伤老年患者的临床资料,其中大连市第四人民医院4例、福建医科大学附属协和医院5例、暨南大学附属广州市红十字会医院22例、黑龙江省医院5例、海军军医大学第一附属医院27例、南昌大学第一附属医院9例、南通大学附属医院10例、武汉大学同仁医院暨武汉市第三医院9例、解放军第924医院12例、张家港市第一人民医院6例、浙江省台州医院4例、郑州市第一人民医院11例。统计患者的总体临床特征,包括性别、年龄、体重指数、烧伤总面积、Ⅲ度烧伤面积、吸入性损伤情况、致伤因素、是否合并基础疾病以及伤后入院时间。根据伤后28 d内的存活结局,将患者分为存活组(89例)与死亡组(35例),比较2组患者基本资料与伤情(同前总体临床特征);伤后第1个24 h凝血指标,包括凝血酶原时间(PT)、活化部分凝血活酶时间(APTT)、凝血酶时间、D-二聚体、纤维蛋白降解产物(FDP)、国际标准化比值(INR)、纤维蛋白原;伤后第1个24 h血常规指标,包括白细胞计数、血小板计数、中性粒细胞与淋巴细胞比值、单核细胞计数、红细胞计数、血红蛋白、血细胞比容;伤后第1个24 h脏器功能指标,包括直接胆红素、总胆红素、尿素、血肌酐、天冬氨酸转氨酶、丙氨酸转氨酶、总蛋白、白蛋白、球蛋白、血糖、甘油三酯、总胆固醇、碱性磷酸酶、肌酸激酶、电解质指标(血钾、血钠、血氯、血钙、血镁及血磷)、尿酸、肌红蛋白、脑钠肽;伤后第1个24 h感染及血气指标,包括降钙素原、C反应蛋白、pH值、氧合指数、碱剩余、乳酸;治疗情况,包括是否行机械通气、是否行连续性肾脏替代治疗、是否行抗凝治疗、是否应用血管活性药及液体复苏情况。筛选影响危重烧伤老年患者伤后28 d死亡的独立危险因素。结果124例患者中男82例、女42例,年龄60~97岁,体重指数23.44(21.09,25.95)kg/m^(2),烧伤总面积54.00%(42.00%,75.00%)体表总面积(TBSA),Ⅲ度烧伤面积25.00%(10.00%,40.00%)TBSA,主要为中重度吸入性损伤,以火焰烧伤者为主,合并基础疾病者43例,以伤后入院时间≤8 h者为主。2组患者年龄,烧伤总面积,Ⅲ度烧伤面积,吸入性损伤情况,伤后第1个24 h PT、APTT、D-二聚体、FDP、INR、白细胞计数、血小板计数、尿素、血肌酐、血糖、血钠、尿酸、肌红蛋白、尿量比较,差异均有统计学意义(Z值分别为2.37、5.49、5.26、5.97、2.18、1.95、2.68、2.68、2.51、2.82、2.14、3.40、5.31、3.41、2.35、3.81、2.16、-3.82,P<0.05);是否行机械通气、是否应用血管活性药比较,差异均有统计学意义(χ^(2)值分别为9.44、28.50,P<0.05)。年龄、烧伤总面积、Ⅲ度烧伤面积、伤后第1个24 h血肌酐、伤后第1个24 h APTT均为危重烧伤老年患者伤后28 d死亡的独立危险因素(比值比分别为1.17、1.10、1.10、1.09、1.27,95%置信区间分别为1.03~1.40、1.04~1.21、1.05~1.19、1.05~1.17、1.07~1.69,P<0.05)。结论危重烧伤老年患者多为火焰烧伤,常伴随中重度吸入性损伤,早期常伴有炎症反应增强、血糖升高、纤溶激活、脏器功能损害等,这与其预后相关。年龄、烧伤总面积、Ⅲ度烧伤面积、伤后第1个24 h血肌酐和APTT是该群体伤后28 d死亡的独立危险因素。

关键词：烧伤老年人预后危险因素回顾性研究

Fe(Ⅲ)-XG配合物选择性絮凝微细粒赤铁矿与石英及其机理研究

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有色金属（选矿部分） 2024年第1期 116-125页

作者：韩英棋杨志超滕青张郭阳方淑华张素红刘生玉太原理工大学矿业工程学院太原030024

基于金属离子配位理论,将FeCl_(3)·6H_(2)O与黄原胶(XG)按一定比例混合制备Fe(Ⅲ)-XG配合物,用于改善微细粒赤铁矿难沉降、回收效果差的问题。采用沉降实验研究了不同条件下Fe(Ⅲ)-XG对微细粒赤铁矿和石英的选择性絮凝行为,结合动电位... 详细信息

基于金属离子配位理论,将FeCl_(3)·6H_(2)O与黄原胶(XG)按一定比例混合制备Fe(Ⅲ)-XG配合物,用于改善微细粒赤铁矿难沉降、回收效果差的问题。采用沉降实验研究了不同条件下Fe(Ⅲ)-XG对微细粒赤铁矿和石英的选择性絮凝行为,结合动电位、红外光谱和显微镜分析,揭示Fe(Ⅲ)-XG对赤铁矿的絮凝作用机理。矿物絮凝沉降试验结果表明:以黄原胶和Fe(Ⅲ)为絮凝剂,无法实现赤铁矿与石英的选择性絮凝,而黄原胶与FeCl_(3)·6H_(2)O质量比为1︰9时生成的Fe(Ⅲ)-XG配位絮凝剂,对赤铁矿和石英表现出较强的选择性絮凝作用,pH值为6时两者沉降率差异最大,分别为91.50%和39.96%。显微镜观察结果证实,Fe(Ⅲ)-XG作用下,赤铁矿颗粒形成块状絮体,且絮体密实程度更大,而石英颗粒间未发生团聚作用,处于相对分散的状态。Zeta电位、溶液化学计算、吸附量实验与红外光谱分析结果表明:黄原胶主要通过羧酸基团的羰基C=O与Fe^(3+)发生配位作用形成Fe(Ⅲ)-XG配合物,Fe(Ⅲ)-XG在石英和赤铁矿表面吸附方式不同,因而在两种矿物表面吸附量不同。Fe(Ⅲ)-XG中的羟基氧和赤铁矿表面的铁元素发生化学键合特异性吸附在赤铁矿表面,而在石英表面只有微弱的氢键吸附。通过FeCl_(3)·6H_(2)O与黄原胶配位组装可显著提升微细粒赤铁矿选择性絮凝分离效果,为赤铁矿选择性絮凝分选提供新的策略和理论指导。

关键词： Fe(Ⅲ)-XG配合物赤铁矿石英絮凝剂选择性絮凝微细粒

Research progress of functional near-infrared spectroscopy in patients with psychiatric disorders

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Forensic Sciences Research 2021年第2期6卷 141-147页

作者： Fan Chang Haozhe Li shengyu Zhang Chen Chen Chao liu Weixiong Cai Shanghai Key Lab of Forensic Medicine Key Lab of Forensic ScienceMinistry of JusticeShanghai Forensic Service PlatformAcademy of Forensic ScienceShanghaiChina School of Mental Health Wenzhou Medical UniversityWenzhouChina

Functional near-infrared spectroscopy(fNIRS)is a technique of detecting cerebral cortical function by using near-infrared light,which is a multifunctional neuroimaging technique and provides a convenient and efficient detection method in neuroscience.In consideration of acceptability,safety,high spatial and temporal resolutions compared with electroencephalogram(EEG)and functional magnetic resonance imaging(fMRI),fNIRS is widely used to study different psychiatric disorders,most prominently affective disorders,schizophrenic illnesses,brain organic mental disorders and neurodevelopmental disorders,etc.The article focuses on the latest research progress and practical application of fNIRS in psychiatric disorders,especially traumatic brain,including studies on the characterization of phenomenology,treatment effects and descriptions of neuroimaging data.

关键词： Forensic sciences psychiatric disorder functional near-infrared spectroscopy traumatic brain injury

A novel Cu-doped high entropy alloy with excellent comprehensive performances for marine application

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Journal of Materials Science & Technology 2021年第10期69卷 48-59页

作者： Yuan Yu Nannan Xu shengyu Zhu Zhuhui Qjao Jianbin Zhang Jun Yang Weimin liu State Key Laboratory of Solid Lubrication Lanzhou Institute of Chemical PhysicsChinese Academy of SciencesLanzhou 730000China Center of Materials Science and Optoelectronics Engineering University of Chinese Academy of SciencesBeijing 100049China Qingdao Center of Resource Chemistry and New Materials Qingdao 266000China School of Public Health Qingdao UniversityQingdao266000China

High entropy alloy(HEA)has attracted great interests as one of the promising multifunctional materials in marine applications.However,Cu as an effective biocide tends to form segregation in HEA,which could deteriorate corrosion and induce brittle fracture.Herein,we report a strategy to tailor the existing form of Cu in HEA from undesired large-scale segregation to uniform distribution with dispersed nanoscale precipitation,while retaining the unique structure characteristics of HEA.Eliminating Cu segregation improves toughness and avoids serious corrosion in the grain boundary.Uniform distribution with dispersed nanoscale precipitation of Cu further enhances the antifouling and lubricating abilities of Cu-doped HEA.Tailored AlCoCrFeNiCu_(0.5)HEA in this work has excellent comprehensive properties combining good mechanical properties,outstanding antifouling abilities,superior resistance to corrosion and wear.Furthermore,the corresponded mechanisms are discussed in terms of Cu-segregation-eliminated,nanoscale-Cu-precipitate-forming and comprehensive properties.

关键词： High entropy alloys Copper Nanoscale precipitate Comprehensive properties Ocean

Investigation of tribological characteristics of nickel alloybased solid-lubricating composites at elevated temperatures under vacuum

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Friction 2021年第5期9卷 990-1001页

作者： Jinming ZHEN Jun CHENG Hui TAN Qichun SUN shengyu ZHU Jun YANG Weimin liu College of Materials Science and Engineering Liaocheng UniversityLiaocheng 252059China State Key Laboratory of Solid Lubrication Lanzhou Institute of Chemical PhysicsChinese Academy of SciencesLanzhou 730000China

The development of a high‐performance solid‐lubricating composite with excellent self‐lubricity over a broad temperature range in vacuum is significant to solve the frictional problems of spatial mechanisms.In this study,the vacuum tribological behaviors of nickel‐matrix/Ag/(Ca,Ba)F2/graphite(0–2 wt%)composites were studied from 25 to 800°C.The results show that the synergistic effects of solid lubricants can significantly improve the tribological properties of the composites in vacuum,with the graphite content contributing considerably.For 2 wt%graphite,a low friction coefficient(0.14–0.25)and the lowest wear rate((0.12–4.78)×10^(-5) mm^(3)∙N^(-1)∙m^(-1))were observed in vacuum over the entire testing temperature range.Moreover,the wear mechanisms were clarified via analysis of the chemical composition and morphologies of the sliding surfaces.

关键词： metal matrix composite graphite solid lubrication vacuum high temperature