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Tryptophan synthase of Phaeophyceae originated from the secondary host nucleus

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Acta Oceanologica Sinica 2014年第2期33卷 63-72页

作者： ZHANG Yalan CHI Shan WU Shuangxiu LIU Cui yu Jun WANG Xumin CHEN shengping LIU Tao College of Marine Life Science Ocean University of China CAS Key Laboratory of Genome Sciences and Information Beijing Key Laboratory if Genome andprecision Medicine TechnologiesBeijing Institute of GenomicsChinese Academy of Sciences Beijing Key Laboratory of Functional Genomics for Dao-di Herbs Beijing Institute of GenomicsChinese Academy of Sciences Guangdong Province Key Laboratory of Functional Molecules in Oceanic Microorganism Zhong Shan School of MedicineSun Yat-Sen University

Tryptophan synthase （TS, EC 4.***.1.20） catalyzes the last two steps of L-tryptophan biosynthesis. In pro-karyotes, tryptophan synthase is a multi-enzyme complex, and it consists ofαandβsubunit which forms anα-ββ-αcomplex. In fungi and diatoms, TS is a bifunctional enzyme. Because of the limited genomic and transcriptomic data of algae, there are few studies on TS evolution of algae. Here we analyzed the data of the 1000 Plants Project （1KP）, and focused on red algae and brown algae. We found out that the TS of Phaeophy-ceae were fusion genes, which probably originated from the secondary host nucleus, and that the TS of Rho-dophyta contained two genes, TSA and TSB, which both display a possible cyanobacterial origin at the time of primary endosymbiosis. In addition, there were two types of TSB genes （TSB1 and TSB2）. Through the multiple sequence alignment of TSB proteins, we found several residues conserved in TSB1 but variable in TSB2 which connect withαsubunit. The phenomenon may suggest that the TSB2 sequences of Rhodophyta cannot form stable complex with TSA.

关键词： tryptophan synthase Rhodophyta Phaeophyceae phylogenetic analysis

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恶性脑肿瘤的药物投递策略

中华脑科疾病与康复杂志（电子版） 2020年第4期10卷 225-229页

作者：李涛张辰林雨于胜平明浩朗任炳成马海文张恺杨学军天津医科大学总医院神经外科天津300052 天津中医药大学第一附属医院外科天津300193

恶性胶质瘤是人类最致命的肿瘤之一,尽管目前手术、放射治疗、化学治疗等治疗方式都取得了进展,但是预后不佳。一些恶性胶质瘤的新型化学药物在实验研究研究中效果明显,但临床疗效却大打折扣,因此,恶性脑肿瘤的药物投递方式也需同步发... 详细信息

恶性胶质瘤是人类最致命的肿瘤之一,尽管目前手术、放射治疗、化学治疗等治疗方式都取得了进展,但是预后不佳。一些恶性胶质瘤的新型化学药物在实验研究研究中效果明显,但临床疗效却大打折扣,因此,恶性脑肿瘤的药物投递方式也需同步发展。本文围绕恶性脑肿瘤的药物投递限制和策略作一综述。

关键词：脑肿瘤药物投递血-脑屏障

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环氧树脂改性水性聚氨酯的性能研究

化学推进剂与高分子材料 2015年第3期13卷 77-80页

作者：张兵余声平韩曦曦鲍俊杰黄毅萍许戈文安徽大学化学化工学院安徽省绿色高分子材料重点实验室安徽合肥230601

以环氧树脂E–44、二苯基甲烷二异氰酸酯(MDI)以及三羟甲基丙烷(TMP)为原料,合成一种端双羟基的扩链剂MET。以异佛尔酮二异氰酸酯(IPDI)、聚己二酸1,4–丁二醇酯(PBA)、1,4–丁二醇(BDO)、二羟甲基丙酸(DMPA)以及MET为主要原料,合成了... 详细信息

以环氧树脂E–44、二苯基甲烷二异氰酸酯(MDI)以及三羟甲基丙烷(TMP)为原料,合成一种端双羟基的扩链剂MET。以异佛尔酮二异氰酸酯(IPDI)、聚己二酸1,4–丁二醇酯(PBA)、1,4–丁二醇(BDO)、二羟甲基丙酸(DMPA)以及MET为主要原料,合成了一系列环氧树脂改性的水性聚氨酯(WPU)乳液。探讨了MET的加入量对WPU性能的影响,通过傅里叶红外光谱(FT–IR)、差示扫描量热(DSC)、热失重(TG)研究了树脂胶膜结构、结晶性和耐热性,并通过拉伸测试研究了改性树脂的力学性能。结果表明:MET引入到聚氨酯分子链上,当w(MET):w(WPU)为5.0:50时,胶膜的最大拉伸强度为42.1 MPa,断裂伸长率为411.8%;MET的加入能明显提高聚氨酯胶膜的热稳定性。

关键词：环氧树脂水性聚氨酯扩链剂改性

Analysis of Saccharina japonica transcriptome using the high-throughput DNA sequencing technique and its vanadium-dependent haloperoxidase gene

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Acta Oceanologica Sinica 2014年第2期33卷 27-36页

作者： LIANG Xiayuan WANG Xumin CHI Shan WU Shuangxiu SUN Jing LIU Cui CHEN shengping yu Jun LIU Tao College of Marine Life Science Ocean University of China CAS Key Laboratory of Genome Sciences and Information Beijing Key Laboratory of Genome andPrecision Medicine TechnologiesBeijing Institute of GenomicsChinese Academy of Sciences Beijing Key Laboratory of Functional Genomics for Dao-di Herbs Beijing Institute of GenomicsChineseAcademy of Sciences Guangdong Province Key Laboratory of Functional Molecules in Oceanic Microorganism Zhong ShanSchool of MedicineSun Yat-Sen University

Saccharina is one of the most important cold-water living marine brown algal genera. In this study we ana-lyzed the transcriptome of S. japonica, which belongs to the 1 000 Plants （OneKP） Project, by using a next-generation high-throughput DNA sequencing technique. About 5.16 GB of raw data were generated, and 65 536 scaffolds with an average length of 454 bp were assembled with SOAP de novo assembly method. In total, 19 040 unigenes were identified by BLAST;25 734 scaffolds were clustered into 37 Gene ontology functional groups;6 760 scaffolds were classified into 25 COG categories, as well as 2 665 scaffolds that were assigned to 306 KEGG pathways. Majority of the unigenes exhibited more similarities to algae including brown algae and diatom than other cyanobacteria, marine diatom, and plant. Saccharina japonica has the outstanding capability to accumulate halogen such as Br and I via halogenation processes from seawater. We acquired 42 different vanadium-dependent haloperoxidases （vHPO） in S. japonica transcriptome data, including 5 segments of vanadium-dependent iodoperoxidase （vIPO） and 37 segments of vanadium-de-pendent bromoperoxidase （vBPO）. Complicated analyses of identified fulllength S. japonica vBPO1 and S. japonica vBPO2 revealed the importance of vBPO among species of brown algae and the strong relationship between marine algal vBPOs and vIPOs. This study will enhance our understanding of the biological charac-teristics and economic values of S. japonica species.

关键词： Saccharina japonica transcriptomic sequencing vanadium-dependent haloperoxidase phylogenic analysis

关于中职学校学生就业能力培养方式与方法的实践与研究

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价值工程 2012年第12期31卷 227-227页

作者：余升平刘淑艳石家庄工程技术学校石家庄050061

本文拟从实践调查与研究的角度分析探索中职学生就业能力培养的理论主张和可行性建议,并以此基础构建基于职业岗位能力的中职学生就业能力理论模型及柱状图测评分析体系,形成较为可行的中职学生就业能力培养模式。

关键词：中职学生就业能力培养模式

Transcriptome characterization of Ishige okamurae(Phaeophyceae)shows strong environmental acclimation

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Acta Oceanologica Sinica 2014年第2期33卷 20-26页

作者： QU Jieqiong WANG Xumin CHI Shan WU Shuangxiu SUN Jing LIU Cui CHEN shengping yu Jun LIU Tao College of Marine Life Science Ocean University of China CAS Key Laboratory of Genome Sciences and Information Beijing Key Laboratory of Genome and Precision Medicine TechnologiesBeijing Institute of GenomicsChinese Academy of Sciences Beijing Key Laboratory of Functional Genomics for Dao-di Herbs Beijing Institute of GenomicsChineseAcademy of Sciences Guangdong Province Key Laboratory of Functional Molecules in Oceanic Microorganism Zhong ShanSchool of MedicineSun Yat-Sen University

Ishige okamurae, with leathery branched narrow fronds consisting of cylindrical hairs, is the typical species of the genus Ishige, which is considered as one of the most basal genera in the phylogeny of the Phaeophy-ceae. Apart from great public interest from the evolutionary respect, more attention has been brought on the abundant bioactive compounds in I. okamurae for therapeutic or economic considerations, such as di-phlorethohydroxycarmalol and ishigoside. Yet little is known about related key genes or metabolic pathways involved in I. okamurae, which calls upon us to carry out global analyses of transcriptome by next generation sequencing. Altogether, we obtained 78 583 assembled scaffolds with N50 of 1 709 nucleotides, and 25 357 unigenes with significant BLAST matches （E-value cutoff of 10-5）. In terms of characterization of the tran-scriptome of I. okamurae, we focused on anti-stress metabolic pathways and synthetic routes of bioactive compounds in an attempt to obtain a better understanding of the interactive organism-environment regula-tory networks. Pathway-based analysis helped us to deepen our comprehension of the interaction between I. okamurae and its surroundings, with MAPK signal pathway as an example. Furthermore, we discovered a wide range of novel putative functional proteins that could be of wide application, such as Rab family, using sequence-based transcriptome. In conclusion, transcriptome characterization of I. okamurae （Phaeophy-ceae） shows strong environmental acclimation.

关键词： Ishige okamurae transcriptome environmental acclimation Rab

Distribution of soil aggregates and organic carbon in deep soil under long-term conservation tillage with residual retention in dryland

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Journal of Arid Land 2019年第2期11卷 241-254页

作者： WANG Bisheng GAO Lili yu Weishui WEI Xueqin LI Jing LI shengping SONG Xiaojun LIANG Guopeng CAI Dianxiong WU Xueping Institute of Agricultural Resources and Regional Planning Chinese Academy of Agricultural Sciences

To ascertain the effects of long-term conservation tillage and residue retention on soil organic carbon(SOC) content and aggregate distribution in a deep soil(>20-cm depth) in a dryland environment,this paper analyzed the SOC and aggregate distribution in soil, and the aggregate-associated organic carbon(OC) and SOC physical fractions. Conservation tillage(reduced tillage with residue incorporated(RT) and no-tillage with residue mulch(NT)) significantly increased SOC sequestration and soil aggregation in deep soil compared with conventional tillage with residue removal(CT). Compared with CT, RT significantly increased the proportion of small macroaggregates by 23%–81% in the 10–80 cm layer, and the OC content in small macroaggregates by 1%–58% in the 0–80 cm layer. RT significantly increased(by 24%–90%) the OC content in mineral-SOC within small macroaggregates in the 0–60 cm layer, while there was a 23%–80% increase in the 0–40 cm layer with NT. These results indicated that:(1) conservation tillage treatments are beneficial for soil aggregation and SOC sequestration in a deep soil in a dryland environment; and(2)the SOC in mineral-associated OC plays important roles in soil aggregation and SOC sequestration. In conclusion, RT with NT is recommended as an agricultural management tool in dryland soils because of its role in improving soil aggregation and SOC sequestration.

关键词： long-term tillage residue retention soil aggregates SOC deep soil dryland

Central role of myeloid MCPIP1 in protecting against LPSinduced inflammation and lung injury

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Signal Transduction and Targeted Therapy 2017年第1期2卷 18-32页

作者： Yong Li Xuan Huang shengping Huang Hui He Tianhua Lei Fatma Saaoud Xiao-Qiang yu Ari Melnick Anil Kumar Christopher J Papasian Daping Fan Mingui Fu Department of Biomedical Science and Shock/Trauma Research Center School of MedicineUniversity of Missouri-Kansas CityKansas CityMissouriMOUSA Institute of Translational Medicine Nanchang UniversityNanchangJiangxiP.R.China Department of Cell Biology and Anatomy University of South Carolina School of MedicineColumbiaSCUSA Division of Molecular Biology and Biochemistry School of Biological SciencesUniversity of Missouri-Kansas CityKansas CityMissouriMOUSA Division of Hematology and Medical Oncology Weill Cornell Medical CollegeNew YorkNYUSA Division of Pharmacology and Toxicology School of PharmacyUniversity of Missouri-Kansas CityKansas CityMissouriMOUSA

Although systemic inflammatory responses attributable to infection may lead to significant lung injury,the precise molecular mechanisms leading to lung damage are poorly understood and therapeutic options remain ***,we show that myeloid monocyte chemotactic protein-inducible protein 1(MCPIP1)plays a central role in protecting against LPS-induced inflammation and lung ***-specific MCPIP1 knockout mice developed spontaneous inflammatory syndromes,but at a late age compared to global MCPIP1 knockout ***,mice with a myeloid-specific deletion of MCPIP1 were extremely sensitive to LPS-induced lung injury due to overproduction of proinflammatory cytokines and *** identified C/EBPβand C/EBPδ,two critical transcriptional factors that drive cytokine production and lung injury,as targets of MCPIP1 *** administration caused MCPIP1 protein degradation in the *** inhibition of MALT1,a paracaspase that cleaves MCPIP1,by MI-2 selectively increased the MCPIP1 protein levels in macrophages and in the ***,administration of MI-2 protected mice from LPS-induced inflammation,lung injury and ***,these results indicate that myeloid MCPIP1 is central in controlling LPS-induced inflammation and lung *** inhibition of MALT1 protease activity may be a good strategy to treat inflammatory diseases by enhancing MCPIP1 expression in myeloid cells.

关键词： inflammation myeloid lung

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含氢原子缺陷晶界的剪切行为

力学学报 2017年第3期49卷 605-615页

作者：赵东伟郁汶山申胜平西安交通大学航天航空学院机械结构强度与振动国家重点实验室/陕西省航天结构振动控制工程实验室西安710049

针对4个α-Fe对称倾斜晶界,采用分子静力学考察了4个晶界中H原子偏析能的分布特征,并采用分子动力学方法研究了晶界内植入不同数量H原子对其在室温条件下剪切行为的影响.H原子通过随机方式植入界面内,利用植入H原子数量与晶界面积的比... 详细信息

针对4个α-Fe对称倾斜晶界,采用分子静力学考察了4个晶界中H原子偏析能的分布特征,并采用分子动力学方法研究了晶界内植入不同数量H原子对其在室温条件下剪切行为的影响.H原子通过随机方式植入界面内,利用植入H原子数量与晶界面积的比值来定义H原子面密度ρ.在含H原子晶界剪切行为分析过程中,重点考察了在不同H原子密度ρ下,4个晶界的初始塑性临界应力和晶界迁移位移的变化趋势以及4个晶界在加载过程中的微观变形机理.研究表明:晶界内的H原子偏析能明显偏低,4个晶界附近的H原子会自发向晶界内偏析;随着植入H原子数量的逐渐增多,晶界的初始塑性临界应力和后续变形阶段应力均会降低.晶界内植入H原子会从本质上改变晶界的微观变形机理,进而影响晶界在外载荷条件下的迁移属性.与不含H原子晶界的变形机理对比发现,加载过程中晶界的微结构会发生剧烈的演化,H原子的扩散和团簇化效应会导致晶界内出现纳米孔缺陷.

关键词：晶界氢原子偏析能剪切行为