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DNA methylation affects photoperiodic tuberization in potato (Solanum tuberosum L.) by mediating the expression of genes related to the photoperiod and GA pathways

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Horticulture Research 2021年第1期8卷 2708-2720页

作者： Yanjun Ai shenglin jing Zhengnan Cheng Botao Song Conghua Xie Jun Liu Jun Zhou Key Laboratory of Potato Biology and Biotechnology Ministry of Agriculture and Rural AffairsHuazhong Agricultural UniversityWuhanHubei 430070China Key Laboratory of Horticultural Plant Biology(HZAU) Ministry of EducationWuhanHubei 430070China College of Life Sciences and Technology Huazhong Agricultural UniversityWuhanHubei 430070China College of Horticulture and Forestry Sciences Huazhong Agricultural UniversityWuhanHubei 430070China Hubei Vocational College of Bio-Technology WuhanHubei 430070China

Overcoming short-day-dependent tuberization to adapt to long-day conditions is critical for the widespread geographical success of *** genetic pathways of photoperiodic tuberization are similar to those of photoperiodic *** methylation plays an important role in photoperiodic ***,little is known about how DNA methylation affects photoperiodic tuberization in ***,we verified the effect of a DNA methylation inhibitor on photoperiodic tuberization and compared the DNA methylation levels and differentially methylated genes(DMGs)in the photoperiodic tuberization process between photoperiod-sensitive and photoperiod-insensitive genotypes,aiming to dissect the role of DNA methylation in the photoperiodic tuberization of *** found that a DNA methylation inhibitor could promote tuber initiation in strict short-day ***-genome DNA methylation sequencing showed that the photoperiod-sensitive and photoperiod-insensitive genotypes had distinct DNA methylation modes in which few differentially methylated genes were *** analysis confirmed that the DNA methylation inhibitor regulated the expression of the key genes involved in the photoperiod and GA pathways to promote tuber initiation in the photoperiod-sensitive *** of the DNA methylation levels and transcriptome levels identified 52 candidate genes regulated by DNA methylation that were predicted to be involved in photoperiodic *** findings provide a new perspective for understanding the relationship between photoperiod-dependent and GA-regulated *** the epigenomic signatures of these pathways will greatly enhance potato breeding for adaptation to a wide range of environments.

关键词： breeding media signature

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Potato tonoplast sugar transporter 1 controls tuber sugar accumulation during postharvest cold storage

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Horticulture Research 2023年第4期10卷 200-210页

作者： Tengfei Liu Md Abu Kawochar Shahnewaz Begum Enshuang Wang Tingting Zhou shenglin jing Tiantian Liu Liu Yu Bihua Nie Botao Song Key Laboratory of Horticultural Plant Biology Ministry of EducationKey Laboratory of Potato Biology and BiotechnologyMinistry of Agriculture and Rural AffairsCollege of Horticulture and Forestry SciencesHuazhong Agricultural UniversityWuhan 430070 China Bangladesh Agricultural Research Institute JoydebpurGazipur 1701Bangladesh

Cold-induced sweetening(CIS),the undesirable sugar accumulation in cold-stored potato(Solanum tuberosum L.)tubers,is a severe postharvest issue in the potato processing *** the process of sucrose hydrolysis by vacuolar invertase during potato CIS is well understood,there is limited knowledge about the transportation of sucrose from the cytosol to the vacuole during postharvest cold ***,we report that among the three potato tonoplast sugar transporters(TSTs),StTST1 exhibits the highest expression in tubers during postharvest cold *** localization analysis demonstrates that StTST1 is a tonoplast-localized ***1 knockdown decreases reducing sugar accumulation in tubers during low-temperature *** to wild-type,potato chips produced from StTST1-silenced tubers displayed significantly lower acrylamide levels and lighter color after cold *** analysis manifests that suppression of StTST1 promotes starch synthesis and inhibits starch degradation in cold-stored *** further establish that the increased sucrose content in the StTST1-silenced tubers might cause a decrease in the ABA content,thereby inhibiting the ABA-signaling *** demonstrate that the down-regulation ofβ-amylase StBAM1 in StTST1-silenced tubers might be directly controlled by ABA-responsive element-binding proteins(AREBs).Altogether,we have shown that StTST1 plays a critical role in sugar accumulation and starchmetabolism regulation during postharvest cold ***,our findings provide a newstrategy to improve the frying quality of cold-stored tubers and reduce the acrylamide content in potato chips.

关键词： sugar acrylamide hydrolysis

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Long-distance control of potato storage organ formation by SELF PRUNING 3D and FLOWERING LOCUS T-like 1

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Plant Communications 2023年第3期4卷 258-273页

作者： SaloméPratand Botao Song shenglin jing Peng Jiang Xiaomeng Sun Liu Yu Enshuang Wang Jun Qin Fei Zhang SaloméPrat Botao Song National Key Laboratory for Germplasm Innovation&Utilization of Horticultural Crops Huazhong Agricultural UniversityWuhanHubei 430070China Key Laboratory of Potato Biology and Biotechnology(HZAU) Ministry of Agriculture and Rural AffairsWuhanHubei 430070China College of Horticulture and Forestry Science Huazhong Agricultural UniversityWuhanHubei 430070China Centro de Investigación en Agrigenomica(CRAG) CSIC-IRTA-UAB-UBCerdanyola08193 BarcelonaSpain

Plants program their meristem-associated developmental switches for timely adaptation to a changing ***(Solanum tuberosum L.)tubers differentiate from specialized belowground branches or stolons through radial expansion of their terminal *** this process,the stolon apex and closest axillary buds enter a dormancy state that leads to tuber eyes,which are reactivated the following spring and generate a clonally identical *** potato FLOWERING LOCUS T homolog SELF-PRUNING 6A(StSP6A)was previously identified as the major tuber-inducing signal that integrates day-length cues to control the storage ***,whether some other long-range signals also act as tuber organogenesis stimuli remains ***,we show that the florigen SELF PRUNING 3D(StSP3D)and FLOWERING LOCUS T-like 1(StFTL1)genes are activated by short days,analogously to *** of StSP3D or StFTL1 promotes tuber formation under non-inductive long days,and the tuber-inducing activity of these proteins is graft *** the non-tuber-bearing wild species Solanum etuberosum,a natural SP6A null mutant,we show that leaf-expressed SP6A is dispensable for StSP3D long-range ***3D and StFTL1 mediate secondary activation of StSP6A in stolon tips,leading to amplification of this tuberigen ***3D and StFTL1 were observed to bind the same protein partners as StSP6A,suggesting that they can also form transcriptionally active ***,our findings show that additional mobile tuber-inducing signals are regulated by the photoperiodic pathway.

关键词： potato Solanum tuberosum L. FLOWERING LOCUS T StSP3D tuberization tuber-inducing signal long-distance signaling

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海藻酸钠/羽毛角蛋白复合纤维的制备与性能

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复合材料学报 2016年第11期33卷 2634-2640页

作者：李圣林郭静杨利军黄学淋牟思阳张森宫玉梅大连工业大学纺织与材料工程学院大连116034 辽宁省功能纤维及其复合材料工程技术中心大连116034

采用酸碱法在鸭毛中提取羽毛角蛋白(FK),通过正交实验研究了FK提取的最佳条件。将FK与海藻酸钠(SA)进行共混,制备SA/FK复合溶液,测试了复合溶液的流变性。通过湿法纺丝制备SA/FK复合纤维,研究了复合纤维的基本性能并表征了复合纤维分子... 详细信息

采用酸碱法在鸭毛中提取羽毛角蛋白(FK),通过正交实验研究了FK提取的最佳条件。将FK与海藻酸钠(SA)进行共混,制备SA/FK复合溶液,测试了复合溶液的流变性。通过湿法纺丝制备SA/FK复合纤维,研究了复合纤维的基本性能并表征了复合纤维分子间氢键作用。结果表明:在最佳提取条件下(提取温度为60.0℃,提取时间为120.00min,碱浓度为2.00wt%),FK产率最高为45.75%。SA/FK复合溶液的表观黏度随剪切速率的增加而减小,随FK含量的增加呈现先增后减的趋势。FK的加入使分子间氢键作用增强。SA/FK复合纤维的力学断裂强度能够达到1.96cN/dtex。SA与FK的复合破坏了原有分子的晶体结构,SA/FK复合纤维分子结构是以非晶态存在。SA/FK复合纤维的表面具有均匀的沟槽结构。

关键词：羽毛角蛋白提取正交实验海藻酸钠复合纤维氢键

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基于热处理CRISPR/Cas9马铃薯植株的基因编辑体系优化

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园艺学报 2023年第12期50卷 2591-2600页

作者：熊文汶刘慧敏陈凯园杜鹃宋波涛景晟林华中农业大学园艺林学学院园艺植物生物学教育部重点试验室农业农村部马铃薯生物学与生物技术重点试验室湖北武汉430070 华中农业大学植物科学技术学院作物遗传改良全国重点实验室湖北武汉430070

以二倍体马铃薯栽培种AC142和四倍体栽培种E3为试验材料,构建并优化了马铃薯内源启动子驱动的CRISPR/Cas9基因编辑体系。以控制白化表型的八氢番茄红素脱氢酶基因(StPDS)为靶标基因,通过农杆菌介导的遗传转化侵染试管薯,根据转化效率和... 详细信息

以二倍体马铃薯栽培种AC142和四倍体栽培种E3为试验材料,构建并优化了马铃薯内源启动子驱动的CRISPR/Cas9基因编辑体系。以控制白化表型的八氢番茄红素脱氢酶基因(StPDS)为靶标基因,通过农杆菌介导的遗传转化侵染试管薯,根据转化效率和表型突变频率的分析,筛选出马铃薯内源启动子StU6和StUBI10驱动的HM4载体为最优CRISPR载体。以常温处理为对照,对HM4获得的AC142的37个全绿株系和白绿嵌合株系、E3的4个全绿株系的侧芽进行循环8次的短期热处理(37℃24h+22℃24h)共15d,表型分析和测序检测表明,对敲除株系进行短期热处理能显著提高CRISPR/Cas9的编辑效率。常温下,AC142和E3敲除株系的表型突变频率分别为16.2%(6/37)和0(0/4),循环8次短期热处理之后,表型突变频率分别提高到54.1%(20/37)和75%(3/4)。其中,AC142敲除株系的侧芽能得到10.8%(4/37)白化株系,而E3未能得到白化株系,只能得到75%(3/4)的白绿嵌合株系。4次连续15 d的短期热处理后,2.1%(3/144)的E3敲除株系的侧芽可在常温下保持白化。对短期热处理后的白化植株进行TA克隆,测序结果显示二倍体敲除株系CR-AC142-StPDS-17/21和四倍体敲除株系CR-E3-StPDS-3/4的8~12个单克隆都不存在野生型,说明本研究的敲除体系可在马铃薯中实现对靶标序列的完全诱导突变。该体系可广泛应用于马铃薯突变体的创制,为马铃薯基因功能组学研究和目标性状精准改良提供技术基础。

关键词：马铃薯基因编辑 CRISPR/Cas9 StPDS 热处理

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糖信号调控马铃薯块茎发育的研究进展

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华中农业大学学报 2021年第4期40卷 27-35页

作者：单建伟柳俊索海翠王丽安康刘计涛景晟林李成晨宋波涛李小波广东省农业科学院作物研究所/广东省农作物遗传改良重点实验室广州510640 华中农业大学生命科学与技术学院武汉430070 华中农业大学园艺林学学院武汉430070

马铃薯是全球最大的非谷类粮食作物,在全球经济和粮食安全中发挥重要作用。块茎是马铃薯的经济器官和繁殖器官,存储了大量淀粉,其发育过程受糖信号调控。糖信号调控马铃薯块茎形态建成及库源关系。本文概述了植物糖信号途径,糖信号调控... 详细信息

马铃薯是全球最大的非谷类粮食作物,在全球经济和粮食安全中发挥重要作用。块茎是马铃薯的经济器官和繁殖器官,存储了大量淀粉,其发育过程受糖信号调控。糖信号调控马铃薯块茎形态建成及库源关系。本文概述了植物糖信号途径,糖信号调控马铃薯块茎形态建成、淀粉积累及其与光周期、植物激素等信号途径的关系等方面的研究进展,并在此基础上提出了糖信号调控马铃薯块茎发育的模式,旨在为进一步阐明糖信号调控马铃薯块茎发育的机制提供借鉴。

关键词：马铃薯块茎发育块茎形态建成库源关系光周期植物激素糖信号

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Review of demand-side energy sharing and collective self-consumption schemes in future power systems

iEnergy

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iEnergy 2023年第2期2卷 119-132页

作者： Jizhong Zhu shenglin Li Alberto Borghetti jing Lan Hong Li Taiheng Guo School of Electric Power Engineering South China University of TechnologyGuangzhou 510641China Department of Electrical Electronic and Information EngineeringUniversity of BolognaBologna 40136Italy

The widespread use of distributed energy sources provides exciting potential for demand-side energy sharing and collective self-consumption ***-side energy sharing and collective self-consumption systems are committed to coordinating the operation of distributed generation,energy storage,and load ***,with the development of Internet technology,sharing economy is rapidly penetrating various *** application of sharing economy in the energy sector enables more and more end-users to participate in energy ***,the deployment of energy sharing technologies poses many *** paper comprehensively reviews recent developments in demand-side energy sharing and collective self-consumption *** definition and classification of sharing economy are presented,with a focus on the applications in the energy sector:virtual power plants,peer-to-peer energy trading,shared energy storage,and microgrid energy sharing *** and future research directions are thoroughly discussed.

关键词： Energy sharing future power systems demand-side energy management distributed energy resources sharing economy

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A channel-confined strategy for synthesizing CoN-CoO_(x)/C as efficient oxygen reduction electrocatalyst for advanced zinc-air batteries

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Nano Research 2022年第3期15卷 2092-2103页

作者： Xingmei Guo Wei Zhang jing Shi Mengting Duan Shanjing Liu Junhao Zhang Yuanjun Liu shenglin Xiong Qinghong Kong School of Environmental and Chemical Engineering Jiangsu University of Science and TechnologyZhenjiang 212003China Key Laboratory of Colloid and Interface Chemistry Ministry of Educationand School of Chemistry and Chemical EngineeringShandong UniversityJinan 250100China School of the Environment and Safety Engineering Jiangsu UniversityZhenjiang 212013China

Designing hybrid transition metal compounds with optimized electronic structure and firmly dispersing them on a matrix to avoid aggregation and shedding is of great significance for achieving high electrocatalytic ***,an adsorption-complexation-calcination strategy based on channel confining effect is explored to obtain CoN-CoO_(x)hybrid nanoparticles uniformly dispersed in mesoporous *** CoN-CoO_(x)/C composite exhibits excellent electrocatalytic behavior for oxygen reduction reaction(ORR).The half-wave potential and durability are comparable or superior to those of Pt/*** applying as cathode catalyst for a primary zinc-air battery,the open-circuit voltage and peak power density reach up to 1.394 V and 109.8 mW·cm^(−2),respectively.A high gravimetric energy density of 950.3 Wh·kgZn^(−1) is delivered at 10 mA·cm^(−2) with good rate capability and *** functional theory(DFT)calculation demonstrates the favorable ORR intermediate adsorbability and metallic characteristics of CoN grains with oxide hybridization to optimize the electronic *** work provides a facile adjustable approach for obtaining highly dispersed nanoparticles with controllable hybrid composition on a substrate,which is important for future design and optimization of high-performance electrocatalysts.

关键词： channel-confined complexation CoN-CoO_(x) mesoporous carbon oxygen reduction reaction zinc-air battery

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Log Anomaly Detection Through GPT-2 for Large Scale Systems

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ZTE Communications 2023年第3期21卷 70-76页

作者： JI Yuhe HAN jing ZHAO Yongxin ZHANG shenglin GONG Zican Nankai University Tianjin 300071China ZTE Corporation Shenzhen 518057China

As the scale of software systems expands,maintaining their stable operation has become an extraordinary *** logs are semi-structured text generated by the recording function in the source code and have important research significance in software service anomaly *** log anomaly detection methods mainly focus on the statistical characteristics of logs,making it difficult to distinguish the semantic differences between normal and abnormal logs,and performing poorly on real-world industrial log *** this paper,we propose an unsupervised framework for log anomaly detection based on generative pre-training-2(GPT-2).We apply our approach to two industrial *** experimental results on two datasets show that our approach outperforms state-of-the-art approaches for log anomaly detection.

关键词： hybrid beamforming hybrid architecture weighted mean square error manifold optimization dynamic subarrays

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Cold sintering process for fabrication of a superhydrophobic ZnO-polytetrafluoroethylene(PTFE)ceramic composite

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Journal of Advanced Ceramics 2023年第9期12卷 1758-1766页

作者： Xuetong Zhao Yang Yang Li Cheng jing Guo shenglin Kang Yuchen Li Xilin Wang Lijun Yang Ruijin Liao State Key Laboratory of Power Transmission Equipment&System Security and New Technology Chongqing UniversityChongqing 400044China State Key Laboratory for Mechanical Behavior of Materials Xi’an Jiaotong UniversityXi’an 710049China Engineering Laboratory of Power Equipment Reliability in Complicated Coastal Environments Tsinghua Shenzhen International Graduate SchoolTsinghua UniversityShenzhen 518055China

Composite coatings or films with polytetrafluoroethylene(PTFE)are typically utilized to offer superhydrophobic ***,the superhydrophobic surfaces usually have limited durability and require complicated fabrication ***,we report the successful integration of PTFE with ZnO ceramics to achieve superhydrophobicity via a one-step sintering method,cold sintering process(CSP),at 300℃.(1–x)ZnO–x PTFE ceramic composites with x ranging from 0 to 70 vol%are densified with relative density of over 97%.Micro/nano-scale PTFE polymer is dispersed among ZnO grains forming polymer grain boundary phases,which modulate surface morphology and surface energy of the ZnO–PTFE ceramic *** the 60 vol%ZnO–40 vol%PTFE ceramic composite,superhydrophobic properties are optimized with static water contact angles(WCAs)and sliding angles(SAs)of 162°and 7°,*** abrading into various thicknesses(2.52,2.26,and 1.99 mm)and contaminating with graphite powders on the surface,WCA and SA are still maintained with a high level of 157°–160°and 7°–9.3°,*** work indicates that CSP provides a promising pathway to integrate polymers with ceramics to realize stable superhydrophobicity.

关键词： cold sintering process(CSP) ZnO-polytetrafluoroethylene(PTFE)ceramic composites contact angles sliding angles(SAs) superhydrophobicity

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