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小檗胺通过抑制JAK2/STAT3信号通路改善小鼠溃疡性结肠炎

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上海中医药大学学报 2023年第2期37卷 14-21页

作者：王鸿卿张丽君林川黄诚范圣洁安叡上海中医药大学中药学院上海201203

目的:观察小檗胺(BBM)对葡聚糖硫酸钠(DSS)诱导的溃疡性结肠炎(UC)小鼠的干预作用及其可能机制。方法:(1)采用RAW264.7巨噬细胞炎症模型,考察BBM对其细胞活力、一氧化氮(NO)释放、肿瘤坏死因子-α(TNF-α)、白介素-1β(IL-1β)、IL-6和... 详细信息

目的:观察小檗胺(BBM)对葡聚糖硫酸钠(DSS)诱导的溃疡性结肠炎(UC)小鼠的干预作用及其可能机制。方法:(1)采用RAW264.7巨噬细胞炎症模型,考察BBM对其细胞活力、一氧化氮(NO)释放、肿瘤坏死因子-α(TNF-α)、白介素-1β(IL-1β)、IL-6和诱导型NO合酶(iNOS)炎症基因表达的影响。(2)雄性C57BL/6小鼠随机分为正常组、模型组、BBM组(50 mg/kg)和柳氮磺胺吡啶(SASP)阳性药组(250 mg/kg),每组8只。建立DSS诱导的UC小鼠模型,连续9 d灌胃给予相应药物。记录小鼠的体质量、脾脏系数、结肠长度及表观,评估疾病活动指数,HE染色观察结肠病理损伤;RT-qPCR检测小鼠结肠中炎症基因TNF-α、IL-1β、IL-6、iNOS的表达;Western blot检测小鼠结肠中紧密连接蛋白Claudin-4、Occludin及JAK2/STAT3通路蛋白的表达。结果:(1)BBM对RAW264.7细胞活力没有明显影响(P>0.05),但能呈剂量依赖性地减少细胞中NO的释放量(P<0.05,P<0.01)及炎症基因TNF-α、IL-1β、IL-6、iNOS的表达(P<0.05,P<0.01);(2)BBM能改善UC小鼠的体质量下降和脾脏系数升高(P<0.05)、抑制结肠的缩短(P<0.05)、降低疾病活动指数评分和改善结肠病理损伤(P<0.05);(3)BBM能减少小鼠结肠中炎症基因TNF-α、IL-1β、IL-6、iNOS的表达(P<0.05,P<0.01);(4)BBM能增加小鼠结肠中Claudin-4、Occludin的表达以及抑制JAK2和STAT3的磷酸化(P<0.05,P<0.01)。结论:BBM能改善DSS诱导小鼠的UC,其作用机制可能为通过抑制JAK2/STAT3信号通路、减少炎症基因的表达,从而增强肠黏膜屏障功能。

关键词：小檗胺溃疡性结肠炎 JAK2/STAT3信号通路

VGWO: Variant Grey Wolf Optimizer with High Accuracy and Low Time Complexity

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Computers, Materials & Continua 2023年第11期77卷 1617-1644页

作者： Junqiang Jiang Zhifang Sun Xiong Jiang shengjie Jin Yinli Jiang Bo fan School of Information Science and Engineering Hunan Institute of Science and TechnologyYueyang414006China Department of Computer and Information Science Linköping UniversityLinköping58183Sweden Department of Safety and Quality Hunan Construction Investment GroupChangsha410004China

The grey wolf optimizer(GWO)is a swarm-based intelligence optimization algorithm by simulating the steps of searching,encircling,and attacking prey in the process of wolf *** with its advantages of simple principle and few parameters setting,GWO bears drawbacks such as low solution accuracy and slow convergence speed.A few recent advanced GWOs are proposed to try to overcome these ***,they are either difficult to apply to large-scale problems due to high time complexity or easily lead to early *** solve the abovementioned issues,a high-accuracy variable grey wolf optimizer(VGWO)with low time complexity is proposed in this *** first uses the symmetrical wolf strategy to generate an initial population of individuals to lay the foundation for the global seek of the algorithm,and then inspired by the simulated annealing algorithm and the differential evolution algorithm,a mutation operation for generating a new mutant individual is performed on three wolves which are randomly selected in the current wolf individuals while after each iteration.A vectorized Manhattan distance calculation method is specifically designed to evaluate the probability of selecting the mutant individual based on its status in the current wolf population for the purpose of dynamically balancing global search and fast convergence capability of VGWO.A series of experiments are conducted on 19 benchmark functions from CEC2014 and CEC2020 and three real-world engineering *** 19 benchmark functions,VGWO’s optimization results place first in 80%of comparisons to the state-of-art GWOs and the CEC2020 competition winner.A further evaluation based on the Friedman test,VGWO also outperforms all other algorithms statistically in terms of robustness with a better average ranking value.

关键词： Intelligence optimization algorithm grey wolf optimizer(GWO) manhattan distance symmetric coordinates

Research advances in pathogenic mechanisms underlying air pollution-induced ocular surface diseases

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Advances in Ophthalmology Practice and Research 2021年第1期1卷 1-4页

作者： fan Song shengjie Hao Yuzhou Gu Ke Yao Qiuli Fu Eye Center of the 2nd Affiliated Hospital School of MedicineZhejiang UniversityHangzhouZhejiang ProvinceChina Zhejiang Provincial Key Lab of Ophthalmology HangzhouZhejiang ProvinceChina

Background:The harmful effect of aerial fine particulate matter(PM_(2.5))has been a serious public health issue and has attracted worldwide attention,especially in developing *** Text:Numerous previous clinical and experimental studies have demonstrated that PM_(2.5) has a clear pathogenic effect on diseases related to the respiratory and cardiovascular *** researches have pointed out that PM_(2.5) plays a pivotal role in the occurrence and progression of ocular surface *** current studies have shown that PM_(2.5) may promote the appearance of conjunctivitis,keratitis,blepharitis,dry eye,meibomian gland dysfunction(MGD)and other ocular surface diseases through regulating a series of mechanisms such as inflammation,immune reaction,oxidative stress,autophagy,cell migration,and ***:This review aims to summarize the current research progress on the pathogenic mechanism of PM_(2.5)-related ocular surface diseases.

关键词： PM_(2.5) Mechanism Ocular surface diseases Air pollutuion

Contribution of Mountain River Materials to the Continental Shelf off Southeastern Hainan Island Since the Mid-Holocene

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Journal of Ocean University of China 2019年第5期18卷 1123-1129页

作者： TIAN Xu JIANG Zuzhou GAO Wei LIU Zhaoqing LIU Xiling fan Ying QIN shengjie JING Chunlei XU Yue LIU Baohua XU fangjian National Deep Sea Center Qingdao 266237China School of Geosciences China University of PetroleumQingdao 266580China Laboratory for Marine Mineral Resources Qingdao National Laboratory for Marine Science and TechnologyQingdao 266071China China Three Gorges New Energy Co. Ltd.Beijing 100053China China National Offshore Oil Corporation International LimitedBeijing 100027China

The concentrations of rare earth elements(REEs)in the bulk sediment of Core X2,which was collected from southeastern Hainan Island,were analyzed to investigate the relative contributions of various provenance regions since *** results show that sediments in Core X2 were primarily derived from Hainan Island with lesser amounts from Taiwan and limited input from the Pearl *** on the application of quantitative inversion to model the REE data,the average contributions of river materials from southeastern Hainan Island and southwestern Taiwan to the study area were 68%and 32%,***,starting at 4.0 kyr BP,the transport of fluvial sediments from Taiwan to the study region increased due to enhanced hydrodynamics in South China Sea(SCS).These results indicate that the contributions of mountain river materials from Hainan Island and Taiwan to the continental shelf of northern SCS are ***,these results demonstrate that mountain rivers can play an important role in the material cycle of continental margins and may feature a greater impact than large river systems in specific continental shelf areas.

关键词： continental shelf South China Sea mid-Holocene rare earth elements provenance

Wx^lv、the Ancestral Allele of Rice Waxy Gene

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Molecular Plant 2019年第8期12卷 1157-1166页

作者： Changquan Zhang Jihui Zhu shengjie Chen Xiaolei fan Qianfeng Li Yan Lu Min Wang Hengxiu Yu Chuandeng Yi Shuzhu Tang Minghong Gu Qiaoquan Liu Key Laboratory of Plant Functional Genomics of the Ministry of Education/Jiangsu Key Laboratory of Crop Genomics and Molecular Breeding College of AgricultureYangzhou UniversityYangzhou 225009China Co-lnnovation Center for Modern Production Technology of Grain Crops of Jiangsu Province/Key Laboratory of Crop Genetics and Physiology of Jiangsu Province Yangzhou UniversityYangzhou 225009China

In rice grains,the Waxy (Wx) gene is responsible for the synthesis of amylose,the most important determinant for eating and cooking *** effects of several Wx alleles on amylose content and the taste of cooked rice have been ***,the relationship between artificial selection and the evolution of various Wx alleles as well as their distribution remain *** we report the identification of an ancestral allele,Wx^lv,which dramatically affects the mouthfeel of rice grains by modulating the size of amylose *** demonstrated that WF originated directly from wild rice,and the three major Wx alleles in cultivated rice (Wx^b,Wx^a,and Wx^in) differentiated after the substitution of one base pair at the functional *** data indicate that the Wx^lv allele played an important role in artificial selection and *** findings also shed light on the evolution of various Wx alleles,which have greatly contributed to improving the eating and cooking quality of rice.

关键词： Oryza sativa Waxy eating and cooking quality allelic variation artificial selection

A rare Waxy allele coordinately improves rice eating and cooking quality and grain transparency

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Journal of Integrative Plant Biology 2021年第5期63卷 889-901页

作者： Changquan Zhang Yong Yang shengjie Chen Xueju Liu Jihui Zhu Lihui Zhou Yan Lu Qianfeng Li Xiaolei fan Shuzhu Tang Minghong Gu Qiaoquan Liu Key Laboratory of Crop Genomics and Molecular Breeding of Jiangsu Province State Key Laboratory of Hybrid RiceYangzhou UniversityYangzhou 225009China Key Laboratory of Plant Functional Genomics of the Ministry of Education/Jiangsu Key Laboratory of Crop Genetics and Physiology/Co‐Innovation Center for Modern Production Technology of Grain Crops of Jiangsu Province Yangzhou UniversityYangzhou 225009China State Key Laboratory of Agrobiotechnology School of Life SciencesThe Chinese University of Hong KongHong KongChina

In rice(Oryza sativa), amylose content(AC) is the major factor that determines eating and cooking quality(ECQ). The diversity in AC is largely attributed to natural allelic variation at the Waxy(Wx)locus. Here we identified a rare Wx allele, Wx^(mw) ,which combines a favorable AC, improved ECQ and grain transparency. Based on a phylogenetic analysis of Wx genomic sequences from 370 rice accessions, we speculated that Wx^(mw) may have derived from recombination between two important natural Wx alleles, Wx^(in) and Wx^(b). We validated the effects of Wx^(mw) on rice grain quality using both transgenic lines and near-isogenic lines(NILs). When introgressed into the japonica Nipponbare(NIP) background, Wx^(mw) resulted in a moderate AC that was intermediate between that of NILs carrying the Wx^(b)allele and NILs with the Wx^(mp) allele. Notably, mature grains of NILs fixed for Wx^(mw) had an improved transparent endosperm relative to soft rice. Further, we introduced Wx^(mw) into a high-yielding japonica cultivar via molecular marker-assisted selection: the introgressed lines exhibited clear improvements in ECQ and endosperm transparency. Our results suggest that Wx^(mw) is a promising allele to improve grain quality, especially ECQ and grain transparency of high-yielding japonica cultivars, in rice breeding programs.

关键词： allelic variation amylose content(AC) eating and cooking quality(ECQ) grain appearance Oryza sativa L. Waxy

Chitosan biopolymer-derived self-powered triboelectric sensor with optimized performance through molecular surface engineering and data-driven learning

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InfoMat 2019年第1期1卷 116-125页

作者： Chenxiang Ma shengjie Gao Xinqi Gao Min Wu Ruoxing Wang Yixiu Wang Zhiyuan Tang Fengru fan Wenxuan Wu Hong Wan Wenzhuo Wu School of Industrial Engineering Purdue UniversityWest LafayetteIndiana Department of Applied Chemistry School of Chemical Engineering and TechnologyTianjin UniversityTianjinChina Flex Laboratory Purdue UniversityWest LafayetteIndiana Department of Chemistry and Biochemistry University of CaliforniaSanta BarbaraCalifornia Department of Research&Development Shenzhen Broadthink Advanced Materials Technologies Co.Ltd.ShenzhenGuangdongChina Edward P.Fitts Department of Industrial&Systems Engineering North Carolina State UniversityRaleighNorth Carolina Birck Nanotechnology Center Purdue UniversityWest LafayetteIndiana Regenstrief Center for Healthcare Engineering Purdue UniversityWest LafayetteIndiana

The state-of-the-art triboelectric nanogenerators(TENGs)are constructed with synthetic polymers,curtailing their application prospects and relevance in sustainable *** economically viable transformation and engineering of naturally abundant materials into efficient TENGs for mechanical energy harvesting is meaningful not only for fundamental scientific exploration,but also for addressing societal *** an abundant natural biopolymer,chitosan enables exciting opportunity for low-cost,biodegradable TENG ***,the electrical outputs of chitosan-based TENGs are low compared with the devices built with synthetic ***,we explore the facile molecular surface engineering in chitosan to significantly boost the performance of chitosan-based TENG for enabling the practical applications,for example,self-powered car speed *** molecular surface engineering offers a potentially promising scheme for designing and implementing high-performance biopolymer-based TENGs for selfpowered nanosystems in sustainable *** further explore for the first time the feasibility of data mining approaches to analyze and learn the acquired triboelectric signals from the car speed sensors and predict the relationship between the triboelectric signals and car speed values.

关键词： performance polymer surface

SNP Detection of High Density Lipoprotein Binding Protein Gene (HBP) and Its Correlations with Growth Traits in Largemouth Bass(Micropterus salmoides)

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Agricultural Biotechnology 2015年第3期4卷 43-46,50页

作者： Chunlong ZHOU Junjie BAI shengjie LI Jiajia fan Dongmei MA Key Lab of Tropical & Subtropical Fishery Resource Application & Cultivation Ministry of Agriculture/Pearl River Fisheries Research InstituteChinese Academy of Fishery Sciences College of Fisheries and Life Science Shanghai Ocean University

High density lipoprotein binding protein （HBP） plays an important role in lipid metabolism of animals. Lipids are indispensable energy materials for fi- shes, especially for carnivorous fishes with low utilization efficiency of carbohydrates. The single nucleotide polymorphism （SNP） of HBP gene may affect the fat metabolism, thereby exerting an effect on the growth traits of largemouth bass （Micropterus salmoides）. Investigating the correlations between SNP and growth traits can provide candidate markers for molecular marker-assisted selection. In this study, partial genomic fraganents of HBP gene （ GenBank accession number： KF652241 ） were amplified based on the sequences of an available contig in the EST-SNP database of largemouth bass. Three SNP mutation loci were identified in the 3＇ non-ceding region of HBP gene by direct sequencing, including H1 （G ＋ 2782T）, 142 （T ＋ 2817C） and H3 （G ＋ 2857A）. Three SNP loci of 165 randomly selected largemouth bass individuals were detected and genotyped by SnaPshot assay. Genetic structure was analyzed by POPGENE32 software. By using spssl7.0 software, a general linear model （GLM） was established for correlation analysis between different genotypes at SNP loci of HBP gene and various growth traits. The results showed that three SNP loci were in Hardy-Weinberg equilibrium state. To be specific, loci H1 and H2 formed two haplotypes （ A and B）, and three geno- types （AA, AB, and BB） were observed; loci H1, H2 and H3 formed six diplotypes （DI, I）2, D3, D4, D5 and D6）. According to the correlations between dif- ferent genotypes and various growth traits, the body weight and total length of largemouth bass individuals with genotype BB were significantly higher than those of individuals with genotype AB （ P 〈 0.05 ） ; the body weight and total length of largemouth bass individuals with diplotype D6 were significantly higher than those of individuals with diplotype D4 （P 〈0.05）. In this study, SNP markers correlated with growth trait

关键词： Largemouth bass (Micropterus sa/mo/des) Single nucleotide polymorphism (SNP) High density lipoprotein binding protein gene (HBP) Haplo- type Diplotype

Diagnostic approaches for monkeypox virus

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iLABMED 2024年第1期2卷 6-13页

作者： Guohao fan Jiahua Kuang shengjie Zhang Yang Yang Yingxia Liu Hongzhou Lu Shenzhen Key Laboratory of Pathogen and Immunity Shenzhen Clinical Research Center for Infectious DiseaseState Key Discipline of Infectious DiseaseShenzhen Third People's HospitalSecond Hospital Affiliated to Southern University of Science and TechnologyShenzhenChina National Clinical Research Center for Infectious Disease ShenzhenChina

Mpox(formerly Monkeypox)is a zoonotic infection caused by Monkeypox virus(MPXV).Since 2022,Mpox epidemics have occurred in many nonendemic countries and regions,leading the World Health Organization to declare a public health emergency of international *** the persistent transmission and evolution of MPXV,symptoms of Mpox have become milder,with some infections being *** addition,MPXV has become more ***,rapid and accurate diagnosis and screening of MPXV is vital to prevent and control MPXV ***,we review and summarize the technical details,application scenarios,and the advantages and disadvantages of MPXV‐specific diagnostic methods.

关键词： antigen diagnosis laboratory testing monkeypox virus specimens