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Biofiltration and disinfection codetermine the bacterial antibiotic resistome in drinking water:A review and meta-analysis

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Frontiers of Environmental Science & Engineering 2020年第1期14卷 23-34页

作者： Kun Wan Wenfang Lin Shuai Zhu shenghua zhang Xin Yu Key Laboratory of Urban Environment and Health.Institute of Urban Environment Chinese Academy of SciencesXiamen 361021China University of Chinese Academy of Sciences Beijing 100049China Beijing Guohuan Tsinghua Environmental Engineering Design&Research Institute Co. Ltd.Beijing 100084China

The bacterial antibiotic resistome(BAR)is one of the most serious contemporary medical *** BAR problem in drinking water is receiving growing *** this study,we focused on the distribution,changes,and health risks of the BAR throughout the drinking water treatment *** extracted the antibiotic resistance gene(ARG)data from recent publications and analyzed ARG profiles based on diversity,absolute abundance,and relative *** absolute abundance of ARG was found to decrease with water treatment processes and was positively correlated with the abundance of 16S rRNA(r=0.963,/?<0.001),indicating that the reduction of ARG concentration was accompanied by decreasing *** treatment processes,biofiltration and chlorination were discovered to play important roles in shaping the bacterial antibiotic *** exhibited positive effects in controlling the diversity of ARG,while biofiltration,especially granular activated carbon filtration,increased the diversity of *** biofiltration and chlorination altered the structure of the resistome by affecting relative ARG *** addition,we analyzed the mechanism behind the impact of biofiltration and chlorination on the bacterial antibiotic *** intercepting influent ARG-carrying bacteria,biofilters can enrich various ARGs and maintain ARGs in *** further selects bacteria co-resistant to chlorine and ***,we proposed the BAR health risks caused by biofiltration and chlorination in water *** reduce potential BAR risk in drinking water,membrane filtration technology and water boiling are recommended at the point of use.

关键词： Drinking water treatment Antibiotic resistance gene Biofiltration Chlorination

Phase Transformation During Intercritical Tempering with High Heating Rate in a Fe-13%Cr-4%Ni-Mo Stainless Steel

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Acta Metallurgica Sinica(English Letters) 2013年第6期26卷 669-674页

作者： Pei WANG shenghua zhang Shanping LU Dianzhong LI Yiyi LI Shenyang National Laboratory for Materials Science Institute of Metal ResearchChinese Academy of Sciences

The phase transformation from martensite to austenite during intercritical tempering with high heating rate in a low carbon martensitic stainless steel Fe-13%Cr-4%Ni-Mo has been investigated to clarify the microstructure evolution in some regions of the weld joint heat affected zone （HAZ）. The experimental results indicate that the start and finish temperatures of the martensite to austenite transformation keep constant when the heating rate is higher than 10 K/s, and the transformation is much faster than nickel diffusion. The mechanism of the martensite to austenite transformation changes from diffusion to diffusionless during the intercritical tempering when the heating rate is higher than 10 K/s. The diffusionless transformation and higher As temperature render it difficult for any austenite to remain at room temperature during the intercritical tempering with high heating rate that occurs in the HAZ. Adding a proper intercritical tempering with low heating rate can induce some reversed austenite in the rapid heated sample.

关键词： Martensitic stainless steel Phase transformation Intercritical tempering Heating rate Microstructure

Lidamycin inhibits tumor growth and pulmonary metastasis in murine breast carcinoma and shows synergy with paclitaxel

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Chinese Science Bulletin 2013年第23期58卷 2805-2811页

作者： HU Lei zhang shenghua SHAO RongGuang ZHEN YongSu Institute of Medicinal Biotechnology Chinese Academy of Medical Sciences & Peking Union Medical College

The main goal of this study was to investigate whether lidamycin (LDM) could enhance the efficacy of paclitaxel (TAX) against breast cancer. In the MTT assay, LDM showed much more potent cytotoxicity than paclitaxel, and there was a synergy on the 4T1/luc breast cancer cells treated with a combination of paclitaxel and LDM. Western blot analysis showed that paclitaxel and LDM synergistically downregulated MMP9, MMP2, VEGF, and upregulated the cleaved PARP proteins. By wound closure cell migration assay, paclitaxel combined LDM obviously inhibited the migration of 4T1/luc cells. At therapeutic dosage level, LDM, paclitaxel, and the combination suppressed the pulmonary metastases by 70.2%, 53.8%, and 88.7%, respectively, and the CDI value was 0.82, indicating synergism. The results show that LDM enhances the antitumor effect of paclitaxel on 4T1/luc breast cancer, in particular, the antimetastatic effects on pulmonary metastasis.

关键词：血管内皮生长因子乳腺癌细胞协同作用力达霉素紫杉醇抑制肿瘤肺小鼠

Antitumor effects of an engineered and energized fusion protein consisting of an anti-CD20 scFv fragment and lidamycin

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Science China(Life Sciences) 2011年第3期54卷 255-262页

作者： FANG Hong MIAO QingFang zhang shenghua CHENG Xin XIONG DongSheng ZHEN YongSu Institute of Medicinal Biotechnology Chinese Academy of Medical Sciences and Peking Union Medical College Beijing 100050 China Institute of Hematology & Hospital of Blood Diseases Chinese Academy of Medical Sciences and Peking Union Medical College Tianjin 300020 China Cancer Institute & Hospital Chinese Academy of Medical Sciences and Peking Union Medical College Beijing 100021 China

Antibody-based fusion proteins are the next generation of antibody therapies for cancer and other ***20 antigen,which is overexpressed on cell membranes in nearly 95% of cases of B-cell Non-Hodgkin's Lymphoma,is an attractive target for the therapy of B-lymphoid *** (LDM) is a potent enediyne-containing antitumor antibiotic that now has entered phase II clinical *** this study,we prepared an engineered fusion protein,scFv-LDP,consisting of an anti-CD20 scFv fragment and the apoprotein LDP of LDM using DNA *** purification and refolding,scFv-LDP was found to bind specifically to CD20-positive lymphoma cells using ELISA and indirect immunofluorescent cytochemical staining *** energized fusion protein scFv-LDP-AE was obtained using molecular reconstitution of the active chromophore AE of LDM and *** assay revealed potent cytotoxicity of scFv-LDP-AE to CD20-positive Raji and Daudi cells,with IC 50 values of 1.21×10-11 and 6.24×10-11 mol L-1,*** in vivo subcutaneous xenograft model of CD20-positive B cell lymphoma in BALB/c (nu/nu) mice was also *** were given intravenously on day 14 and 21 after tumor *** terms of maximal tolerated doses,scFv-LDP-AE at 0.3 mg kg-1 suppressed tumor growth by 79.3%,and LDM at 0.05 mg kg-1 by 68.6% (P<0.05).Results suggested scFv-LDP-AE could be a potential candidate for tumor-targeting therapy.

关键词： lymphoma CD20 lidamycin scFv energized fusion protein

Responses of bacterial strains isolated from drinking water environments to N-acyI-L-homoserine lactones and their analogs during biofilm formation

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Frontiers of Environmental Science & Engineering 2014年第2期8卷 205-214页

作者： Zhuoying WU1 Qing WANG, Feng GUO shenghua zhang Qipei JIANG Xin YU Key Laboratory of Urban Environment and Health Institute of Urban Environment Chinese Academy of Sciences Xiamen 361021 China

Often as a result ofbiofilm formation, drinking water distribution systems （DWDS） are regularly faced with the problem of microbial contamination. Quorum sensing （QS） systems play a marked role in the regulation of microbial biofilm formation; thus, inhibition of QS systems may provide a promising approach to biofilm formation control in DWDS. In the present study, 22 bacterial strains were isolated from drinking water-related environments. The following properties of the strains were investigated： bacterial biofilm formation capacity, QS signal molecule N-acyl-L-homoserine lactones （AHLs） production ability, and responses to AHLs and AHL analogs, 3-chloro-4-（dichloromethyl）-5-hydroxy-2（5H）- furanone （MX） and 2（5H）-furanone. Four AHLs were added to developed biofilms at dosages ranging from 0.1 nmol.L J to 100nmol.L1. As a result, the biofilm growth of more than 1/4 of the isolates, which included AHL producers and non-producers, were significantly promoted. Further, the biofilm biomasses were closely associated with respective AHLs concentrations. These results provided evidence to support the idea that AHLs play a definitive role in biofilm formation in many of the studied bacteria. Meanwhile, two AHLs analogs demon- strated unexpectedly minimal negative effects on biofilm formation. This suggested that, in order to find an applicable QS inhibition approach for biofilm control in DWDS, the testing and analysis of more analogs is needed.

关键词： drinking water distribution systems (DWDS) biofilm quorum sensing (QS) N-acyl-L-homoserine lac- tones (AHLs) (dichloromethyl)-5-hydroxy-2(5H)-furanone (MX) 2(5H)-furanone

Characterization of extracellular polymeric substances in the biofilms of typical bacteria by the sulfur K-edge XANES spectroscopy

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Journal of Environmental Sciences 2014年第8期26卷 1763-1768页

作者： Huirong Lin Chengsong Ye Lu Lv Clark Renjun Zheng shenghua zhang Lei Zheng Yidong Zhao Xin Yu Institute of Urban Environment Chinese Academy of Science Beijing No.4 High School Institute of High Energy Physics Chinese Academy of ScienceBeijing Synchrotron Radiation Facility

A combined approach of physicochemical extraction and sulfur K-edge X-ray absorption near-edge structure（XANES） spectroscopy was applied to characterize the extracellular polymeric substances（EPS） of typical bacterial biofilms in this study. Physicochemical analysis showed variation of the contents of DNA,polysaccharide and protein in different fractions of EPS in different mediums. The sulfur K-edge XANES analysis yielded a variety of *** fitting of the XANES spectra utilizing a large set of model compounds showed that there was more reduced sulfur in both LB-EPS（loosely bound EPS） and TB-EPS（tightly bound EPS） of all the biofilms in LB medium than in R2 A medium. More oxidized sulfur was identified in LB-EPS than that in TB-EPS,suggesting different niches and physiological heterogeneity in the biofilms. Our results suggested that the sulfur K-edge XANES can be a useful tool to analyze the sulfur speciation in EPS of biofilms.

关键词： Biofilms Characterization EPS Sulfur XANES

RasGAP-derived peptide GAP159 enhances cisplatin-induced cytotoxicity and apoptosis in HCT116 cells

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Acta Pharmaceutica Sinica B 2014年第2期4卷 128-134页

作者： Hao zhang shenghua zhang Hongwei He Caixia zhang Yi Chen Dongke Yu Jianhua Chen Rongguang Shao Institute of Medicinal Biotechnology Chinese Academy of Medical Sciences&Peking Union Medical CollegeBeijing 100050China Wuhan KatyGen Pharmaceuticals Inc.Wuhan 430074China

To increase the efficacy of currently used anti-cancer genotoxins,one of the current efforts is to find agents that can sensitize cancer cells to genotoxins so that the efficacious doses of genotoxins can be lowered to reduce deleterious *** this study,we reported that a synthetic RasGAP-derived peptide GAP159 could enhance the effect of chemotherapeutic agent cisplatin(CDDP)in human colon carcinoma HCT116 *** results showed that GAP159 significantly increased the CDDP-induced cytotoxicity and apoptosis in HCT116 *** synergistic effect was associated with the inhibitions of phospho-AKT,phospho-ERK and NF-κ*** mouse colon tumor CT26 animal models,GAP159 combined with CDDP significantly suppressed CT26 tumor growth,and GAP159 alone showed slight inhibitory *** data suggests that co-treatment of GAP159 and chemotherapeutics will become a potential therapeutic strategy for colon cancers.

关键词： GAP159 CDDP Chemosensitization Colon cancer Apoptosis

Phytosterol of Potamogeton maackianus and Its Change under the Stress of Microcystis aeruginosa

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Wuhan University Journal of Natural Sciences 2008年第1期13卷 113-117页

作者： zhang shenghua WU Zhenbin CHENG Shuiping LIANG Wei WU Xiaohui Institute of Hydrobiology Chinese Academy of Sciences / State Key Laboratory of Freshwater Ecology and Biotechnology Wuhan 430072 Hubei China Graduate University of Chinese Academy of Sciences Beijing 100049 China

The phytosterol in Potamogeton maackianus was identified and quantified. From the hexane extracts analysis of unsaponifiable fraction ofP. maackianus by gas chromatography - mass spectrometry（GC-MS）, campesterol, stigmasterol and β-sitosterol were the main phytosterols in P. maackianus with the contents of 0.368, 1.17 and 0.824 mg·g^1, respectively. After 7 days＇ exposure under M. aeruginosa, the contents of campesterol, stigmasterol and β-sitosterol in *** increased by 23.1%, 40.4% and 40.8%, respectively. Both the contents of 24-ethyl sterols （sitosterol and stigmasterol） and 24-methyl sterol （campesterol） in macrophytes increased, but the percentage of total sterol composition did not change significantly. This result indicated that the content of 4-demethyl sterols and the second alkylation of the phytosterol side-chain at C-24 in P. maackianus were affected by M, aeruginosa.

关键词： Potamogeton maackianus Microcystis aeruginosa phytosterol enviromental stress

Lidamycin Induces Apoptosis of B-Cell Lymphoma Cells and Inhibits Xenograft Growth in Nude Mice

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Clinical oncology and cancer researeh 2009年第3期6卷 203-207页

作者： Hong Fang shenghua zhang Qingfang Miao Dongsheng Xiong Yongsu Zhen Institute of Medicinal Biotechnology Chinese Academy of Medical Sciences and Peking Union Medical College (CAMS & PUMC) Beijing 100050 China Institute of Hematology CAMS & PUMC Tianjin 300020 China Cancer Institute & Hospital CAMS & PUMC Beijing 100021 China

OBJECTIVE To study the cytotoxicity of Lidamycin （LDM） and its induction of apoptosis in Raji and Daudi cells of B-cell lymphoma, and the inhibition of growth of the lymphoma Raji xenograft in nude mice. METHODS MTT assay was used to observe the inhibition by LDM on the proliferation of the Raji and Daudi ceils. Annexin V-FITC/PI double-stain, in combination with flow cytometry （FCM）, was used to determine the induction of apoptosis by LDM in Raji cells. The B-cell lymphoma Raji xenograft model in nude mice was set up to detect the in vivo antitumor activity of LDM. RESULTS LDM markedly inhibited the proliferation of the Raji and Daudi cells in vitro, with IC50 values of 7.13×10^-11 mol/L and 2.91×10^-10 mol/L, respectively. The apoptotic rates of Raji cells were respectively 77.98% and 67.63% at 0.5 nmol/L and 0.25 nmol/L of LDM, indicating an obvious induction of apoptosis in Raji cells. LDM inhibited the formation and growth of human B-cell lymphoma Raji xenograft in nude mice. The inhibition rates of tumor growth were respectively 74.9% and 65.2% in LDM at dosage group of 0.05 mg/kg and 0.025 mg/kg, suggesting an apparent prolongation of survival time in the nude mouse bearing lymphoma. CONCLUSION LDM can effectively induce apoptosis of the B-cell lymphoma cells and inhibit the xenograft growth in nude mice.

关键词： lidamycin (LDM) lymphoma B-cell apoptosis.