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Identification Cold Tolerance of Pineapple Germplasms at Seedling Stage

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American Journal of Plant Sciences 2021年第12期12卷 1768-1779页

作者： Haiyan Shu Keming Li Yanfei Ou Rulin Zhan shenghe chang Haikou Experimental Station Chinese Academy of Tropical Agricultural Sciences Haikou China

Pineapple plant was always injured due to low temperature. Breeding varieties with high cold tolerance was the supreme way to resolve this problem and identifying cold tolerance of germplasms collected was necessary. In this study, SSR locus around homologous sequences of cold-tolerant genes was searched and screened using MD-2 (cold-tolerant variety) and Tainong 17 (cold-sensitive variety). Seventy three pairs of primers of which PCR results were different between these two varieties were gotten. PCRs were performed using these as primers and genomic DNA of germplasms collected as templates. Six pairs of primers were found that their PCR results were in good consistent with cold tolerance. Using GLM (General linear model) association mapping analysis, two SSR markers linked to cold tolerance were found. Their efficiency was verified further using pineapple germplasms with high cold tolerance which had been validated in field by cold wave. They will be used in cold-tolerant breeding of pineapple in the future.

关键词： Pineapple Cold Tolerance Seedling Stage Simple Sequence Repeat (SSR) Breeding

The Situation and Challenges of Pineapple Industry in China

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Agricultural Sciences 2019年第5期10卷 683-688页

作者： Haiyan Shu Wei Sun Guiying Xu Rulin Zhan shenghe chang Haikou Experimental Station Chinese Academy of Tropical Agricultural Sciences Haikou China

Pineapple is the third largest tropical fruit in China. Pineapple industry played important roles in developing economies in tropical- and subtropical-areas in China. However, the structure of varieties is simple. The harvesting period is centralized. The price of pineapple fruits is often low. These challenges hindered the further development of pineapple industry. The people engaged in pineapple industry should select and cultivate new varieties with higher qualities and that can be harvested in a longer period. More work should be done in the technology on inducing inflorescence of new varieties. Precision fertilization for new varieties should be researched and developed. The technology for cultivating tissue-culture seedlings fast and at low cost should also be studied. The diseases in fruits should be prevented and controlled before harvesting.

关键词： Pineapple Situation Challenge Variety Disease

A Simple Method for Pineapple Seedling Propagation

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Agricultural Sciences 2019年第12期10卷 1534-1541页

作者： Haiyan Shu Keming Li Wei Sun Guiying Xu Rulin Zhan shenghe chang Haikou Experimental Station Chinese Academy of Tropical Agricultural Science Haikou China

Pineapple is nutrient and planted in many tropical and subtropical countries. Plenty of elite seedlings were always needed in short time. The common methods for propagating pineapple seedlings have low-multiplying index or take long time. A new method was introduced in this paper. Shoots were induced using stem sections. And then, the shoot bases were cut and cultured on callus-induced medium. The meristematic granules induced were cultured on shoot-induced medium. Shoots can be induced from the meristematic granules. Seedlings can be induced from these shoots in short time. This method overcomes the defects of the common ways. Enough seedlings can be gotten in short time. The operation is simple and easy to learn.

关键词： Pineapple Seedling Caespitose Pathway Callus Pathway

The Responses of Pineapple Varieties to Forced-Flower Treatment at Seedling Stage

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American Journal of Plant Sciences 2019年第12期10卷 2151-2158页

作者： Haiyan Shu Keming Li Wei Sun Guiying Xu Rulin Zhan shenghe chang Haikou Experimental Station Chinese Academy of Tropical Agricultural Science Haikou China

Forcing flower is an important step for planting pineapple. Although many papers have been reported about forcing flower for pineapple plants on the aim of harvesting fruits, no reports have been found about forcing flower at seedling phase for preparing bonsai. In this paper, Tainong 4, Tainong 16 and Tainong 17 plants with 20 leaves were watered on stem apex with 40 ml of 0.13% of ethephron. Results showed that most of Tainong 4 plants flowered after being treated 30 days. None plant of Tainong 16 flowered after being treated 60 days. In the same variety, for example, Tainong 4, some plants flowered earlier, some plants flowered late. Tainong 4 was the most sensitive to ethephron at early-growth phase. Tainong 16 was the most insensitive variety. The low flower rate in this research might be due to that the pineapple plants were in early-vegetative-growth stage. They had not appropriate physiological conditions for forcing flowers. For making pineapple bonsai at early-vegetative-growth phase, higher doses of forcing-flower agent should be used than those of forcing-flower agent for harvesting fruits.

关键词： Pineapple Flower Ethephron Vegetative-Growth Phase

The Cause for Water-Heart Fruit of Pineapple and Protective Measurements

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American Journal of Plant Sciences 2019年第6期10卷 885-892页

作者： Haiyan Shu Wei Sun Keming Li Guiying Xu Rulin Zhan shenghe chang Haikou Experimental Station Chinese Academy of Tropical Agricultural Sciences Haikou China

Pineapple industry has played important roles in China’s economics in tropical- and subtropical-areas. Pineapple industry in China has been damaged by water-heart fruit. The objective of this research is to clarify the mechanism underlying water-heart fruit of pineapple and find ways for preventing water-heart fruit. We found that water logging can cause water-heart pineapple. The longer the pineapple plants were water logged, the more fruits were found water heart. With the development of fruits, the fruits’ sensitivity to water logging increased. Spraying potassium sulfate onto the fruits after the plants are water logged can decrease the rate of pineapple water-heart. But this effect became less with the fruit development. When sucrose and its precursors in intracellular space were transferred into sink cells, hydrogen ions were required. Because of lacking hydrogen ions, sucrose and its precursors were stopped in intracellular space and seized water from cells. Water-heart fruit formed. If potassium can be gotten in intracellular space, the roles of hydrogen ions might be replaced with potassium ions. With the help of potassium ions, sucrose and its precursors were transported into sink cells and the water-heart symptoms alleviated.

关键词： Water-Heart Fruit Pineapple Water Logging Potassium Hydrogen Ions

A Method for Prolonging Culturing Time of Banana Seedling in Nursery Shed

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Advances in Bioscience and Biotechnology 2019年第5期10卷 119-131页

作者： shenghe chang Zhengjing Wu Qi Zeng Jingyi Zhang Wei Sun Lan Qiao Haiyan Shu Haikou Experimental Station Chinese Academy of Tropical Agricultural Sciences Haikou China The Key Lab of Hainan Banana Genetics and Breeding Haikou China School of Forestry Henan University of Science and Technology Luoyang China

Banana industry plays important roles in the economics of southern China, where is always blown by typhoon in summer and autumn. After typhoon passed through, fresh banana seedlings are often required. That is a problem for companies on culturing banana seedling, since culturing banana seedlings takes about four months and typhoon cannot be predicted so early. If the growth of banana seedlings can be slowed down and the seedlings can be cultured in nursery shed for more time, this problem will be solved. In this research, seven treatments (paclobutrazol, diniconazole, paclobutrazol + brassins, paclobutrazol + diethyl aminoethyl hexanoate, diniconazole + brassins, diniconazole + diethyl aminoethyl hexanoate, chlormequat chloride) were performed on banana seedlings and the growth was measured. Results showed that in all of the treatments, the values of plant height, leaf length, pseudostem diameters, root length, root weight, root volume, fresh weight of above-ground tissues and fresh weight of seedlings were significantly less than those corresponding values of control. The reducing powers of roots of seedlings sprayed with paclobutrazol and paclobutrazol + diethyl aminoethyl hexanoate were remarkably higher than those of seedlings treated with water. Total chlorophyll content in leaves of seedlings sprayed with paclobutrazol was remarkably higher than that of seedlings treated with water. Total chlorophyll contents in leaves of seedlings treated with paclobutrazol + diethyl aminoethyl hexanoate and chlormequat chloride were lower than those of control. For delaying the growth of banana seedlings, chlormequat chloride was the best among the seven treatments. The mechanism might be that the enzyme CPP-synthase in seedlings was inhibited by chlormequat chloride.

关键词： Banana Tissue-Culturing Seedling Plant Retardant Gibberellin

Accumulation of Sugars and Liquid in Apoplast of Fruit Flesh Result in Pineapple Translucency

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American Journal of Plant Sciences 2022年第5期13卷 576-587页

作者： Haiyan Shu You Wang Keming Li Luqiong He Lifen Ding Rulin Zhan shenghe chang Haikou Experimental Station Chinese Academy of Tropical Agricultural Sciences Haikou China College of Tropical Crops Yunnan Agricultural University Puer China

Translucency is a recurring problem for pineapple industry. Translucent fruit contained more sucrose, glucose and fructose in apoplast than those in apoplast of normal fruit. There were more liquid in intercellular space of translucent fruit than that of normal flesh. The contents of alcohol and ethylene in translucent fruit were higher than those in normal fruit. Translucent fruit contained less calcium than normal fruit. Electrolyte leakage of translucent flesh was more than that of normal flesh. There were 205 proteins of which the expressions in translucent flesh were higher than those in normal flesh. Calcium-ions-binding protein EF-hand domain-containing protein, ethylene-synthesizing enzyme 1-aminpcyclopropane-1-carboxylate oxidase, ROS-producing protein universal stress protein A-like protein were the top three proteins of which the expressions in translucent flesh were higher than those in normal fruit. When much sugar was transferred into fruit pulp and accumulated in intercellular space, water will be absorbed from cells around and translucence formed. The accumulation of sugar and liquid in apoplast were due to that cell wall and membrane were degraded, which was from being attacked by ROS. There might be more and larger pores in cell wall and membranes of translucent flesh. These data played foundations for researching methods for controlling pineapple translucency.

关键词： Pineapple Translucency Accumulation Sugars Apoplast

The Effects for Delaying Banana Seedling Growth through Spraying Growing Retardants on Stem Apex

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American Journal of Plant Sciences 2019年第5期10卷 813-825页

Replanting is always required for banana farmers. Selecting growth retardants is important for banana seedling companies. Stem apex contained many gibberellins. Seven treatments were performed onto stem apex of banana seedlings in this research. Results showed that heights of seedlings sprayed with paclobutrazol (200 mg/L), dniconazole (200 mg/L), and dniconazole + brassins (200 mg/L + 0.1 mg/L) were significantly lower than those of seedlings sprayed with water. Paclobutrazol was the ideal retardant for delaying the growth of leaf if stem apex was selected as a treated candidate. Fresh weights of seedlings of all seven treatments were significantly less than that of control. For delaying the growth of fresh weight of banana seedlings, chlormequat chloride (200 mg/L) was the best choice. Reducing power of roots of seedlings treated with 100 ml of paclobutrazol (200 mg/L) was significantly higher than that of seedling treated with water. Chlorophyll contents of seedlings treated with growth retardants were significantly higher than that of seedlings treated with water.

关键词： Banana Seedlings Growth Retardants Selecting Gibberellins

A Method to Predict Banana Pseudostem’s Strength at Seedling Stage

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Advances in Bioscience and Biotechnology 2018年第9期9卷 391-396页

作者： shenghe chang Zhengjing Wu Wei Sun Lan Qiao Qi Zeng Jingyi Zhang Haiyan Shu Haikou Experimental Station Chinese Academy of Tropical Agricultural Sciences Haikou China The Key Laboratory of Hainan Banana Genetics and Breeding Haikou China School of Forestry Henan University of Science and Technology Luoyang China

Banana industry played important roles in Chinese economics. Banana production was always affected heavily by typhoon. Selecting new cultivars with high strength was the basic way to resolve this problem. If the selecting work was performed at mature stage, it will need large area and much labor. If this work can be done at seedling stage, this process will be simplified significantly. We found that banana plants that had high strength at seedling stage always had high strength at mature stage. The physical strength of seedling pseudostem was closely related with pseudostem diameter, lignin content and the expression of 4-coumarate:CoA ligase gene Mu4CL15. Those seedlings whose pseudostem had bigger diameter, more lignin content and higher expression of Mu4CL15 always had higher strength to resist lodging. Through screening plants whose pseudostem had bigger diameter, more lignin content and higher expression of Mu4CL15, plants with high-strength pseudostem can be selected. Using this method, thousands of seedlings can be identified in short time. This process does not need large area and much labor. If this method was applied, the process of breeding new cultivars of banana with high resistance to lodging will be accelerated.

关键词： Banana Lodging Identifying Seedling Stage