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A clinicopathological analysis in unsuspected gallbladder carcinoma: A report of 23 cases

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World Journal of Gastroenterology 2007年第12期13卷 1857-1861页

作者： Li-Ning Xu sheng-quan zou Department of Hepatobiliary Surgery of PLA General Hospital Beijing 100853 China Department of General Surgery of Tongji Hospital Tongji Medical College of Huazhong University of Science and Technology Wuhan 430030 Hubei Province China

AIM： To study the clinicopathological characteristics of unsuspected gallbladder carcinoma （UGC）. METHODS： We retrospectively studied 23 cases of UGC in Tongji Hospital, and compared their clinicopathological characteristics with 33 cases of preoperatively diagnosed gallbladder carcinoma （PDGC）. RESULTS： The proportion of UGC coexisting with cholecystolithiasis was significantly higher than that of PDGC （x^2 = 13.53, P 〈 0.01）. The infection rate of hepatitis B virus was 21.74% （5/23） in UGC and 30.30% （10/33） in PDGC. Nine （39.13%） of 23 patients with UGC and 8/33 （24.24） PDGC had contact with schistosome pestilent water. The rate of multiple pregnancies was 56.52% （13/23） in the patients with UGC and 42.42% （14/33） in PDGC. The primary location of the UGC was mostly in the neck and body of the gallbladder, and that of the PDGC was often in the body and bottom. The incidence of Nevin stage I and 11 UGC was significantly higher than that of PDGC （x^2 = 4.44, P 〈 0.05 and 2 = 4.96, P 〈 0.05） while that of Nevin stage V UGC was significantly lower than that of PDGC （x^2 = 7.59, P 〈 0.01）. According to the grading of carcinoma, the incidence of well-differentiated UGC was significantly higher than that of PDGC （2 = 4.16, P 〈 0.05）, and that of poorlydifferentiated UGC was significantly lower than that of PDGC （x^2 = 4.48, P 〈 0.05）.CONCLUSION： There are different characteristics between UGC and PDGC, such as in primary location, malignant degree and incidence of coexistence with cholecystolithiasis. Cholecystolithiasis, hepatitis B, schistosome and multiple pregnancies were high risk factors for gallbladder carcinoma.

关键词： Gallbladder neoplasms Clinical pathology Diagnostic techniques and procedures Diagnostic errors Surgery

Hepatic injury in rats with obstructive jaundice: roles of the protein kinase C signal pathway and cytoprotection of fructose

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Hepatobiliary & Pancreatic Diseases International 2005年第4期4卷 577-581页

作者： Jian-Ming Wang Hui Wang Li-Ning Xu sheng-quan zou Department of General Surgery Tongji HospitalTongji Medical College Huazhong University of Science and Technology Wuhan 430030 China. wangjm18@***

BACKGROUND: Fructose is cytoprotective during bile salt-induced apoptosis of hepatocytes by regulating protein kinase C (PKC). This study was undertaken to explore the regulating mechanism of hepatic injury in rats with obstructive jaundice, and to detect the PKC signal pathway. METHODS: Rat hepatocytes were isolated by in situ colla-genase perfusion and primary culture, and pretreated with various concentrations of PKC agonist phorbol myristate acetale (PMA) and inhibitor chelerythrine for 20 minutes. After pretreatment, 50 μmol/L glycochenodeoxycholate (GCDC) was added for additional 24 hours. Subsequently, the cells were detected by FCM and TUNEL. After adding with different concentrations of fructose and 100 μmol GCDC , the hepatocytes were evaluated by FCM and TUNEL. Experimental obstructive jaundice was induced with fructose and without fructose via double ligation of the bile duct for 3, 7, 14, and 21 days. Apoptotic status in the liver of all rats was detected with TUNEL, and PKC protein in the liver of obstructive jaundice ( OJ) with the immunohisto-chemistry method. RESULTS: PMA increased GCDC-induced apoptosis and chelerythrine decreased GCDC-induced apoptosis in a concentration-dependent manner. Adding with different concentration of fructose and 100 μmol GCDC, the decreased apoptotic rate was related to the concentration of fructose. The apoptotic rate of the liver was related to times of OJ. PKC and apoptosis index (AI) were the highest after a 14-day ligation of the bile duct without use of fructose. AI and PKC were decreasing from a 14-day ligation of the bile duct with fructose. CONCLUSIONS: PKC takes part in the regulation, occurrence , and progression of hepatic injury in OJ. Fructose is cytoprotective during bile salt-induced apoptosis of hepato-cytes by regulating PKC.

关键词： cholestasis hepatic injury protein kinase C fructose

Upreguiation of human telomerase reverse transcriptase mRNA expression by in vitro transfection of hepatitis B virus X gene into human hepatocarcinoma and cholangiocarcinoma cells

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World Journal of Gastroenterology 2005年第36期11卷 5627-5632页

作者： Zhen-Liang Qu sheng-quan zou Nai-Qiang Cui Xian-Zhong Wu Ming-Fang Qin Di Kong Zhen-Li Zhou Department of surgery Tianjin Nankai Hospital Tianjin 300100 China Department of Surgery Tongji Hospital Wuhan430030 Hubei Province China

AIM： To study the changes of human telomerase reverse transcriptase （hTERT） mRNA expression in human hepatocarcinoma cell lines （HepG2） and cholangiocarcinoma cell lines （QBC939） after HBx gene transfection and to illustrate the significance of transcriptional regulation of hTERT gene by HBx gene in the carcinogenesis. METHODS： HepG2 and QBC939 cell lines were cultured and co-transfected with eukaryotic expression vector containing the HBx coding region and cloning vector containing enhanced green fluorescent protein （EGFP） coding sequence using lipid-mediated gene transduction technique. Thirty-six hours after transfection, EGFP expression in cells was used as the indicator of successful transfection. Flow cytometry was performed to determine the transfection efficiency. Cells were harvested and total RNA was extracted using TRIzol reagent. The expression of hTERT mRNA in HepG2 and QBC939 cell lines was assayed by reverse transcriptionpolymerase chain reaction. The expression of HBx protein in both cell lines was detected by immunocytochemical staining and Western blotting. RESULTS： Flow cytometry showed that the transfection efficiency was 46.4% in HepG2 cells and 29.6% in QBC939 cells for both HBx gene expression vector and blank vector. The expression of hTERT mRNA was meaningfully increased in HepG2 and QBC939 cell lines when transfected with HBx gene expression vector compared to those transfected with OPTI-MEM medium and blank vector. Immunocytochemical staining and Western blotting revealed HBx protein expression in HepG2 and QBC939 cells only when transfected with HBx gene. CONCLUSION： HBx gene transfection can upregulate the transcriptional expression of hTERT mRNA. The transactivation of hTERT gene by HBx gene is a newfound mechanism for pathogenesis of hepatocarcinomas and cholangiocarcinomas after HBV infection. 2005 The WJG Press and Elsevier Inc. All rights reserved

关键词： Hepatocholangiocarcinoma Human telomerasereverse transcriptase Gene expression Hepatitis B virus X protein

Effect of histone deacetylase inhibitor on proliferation of biliary tract cancer cell lines

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World Journal of Gastroenterology 2008年第16期14卷 2578-2581页

作者： Li-Ning Xu Xin Wang sheng-quan zou Institute of Hepatobiliary Surgery of PLA GeneralHospital Beijing 100853 China Department of General Surgery of Tongji Hospital Tongji Medical College Huazhong University of Science and Technology Wuhan 430030 Hubei Province China

AIM: To explore the effect of histone deacetylase inhibitor, trichostatin A (TSA) on the growth of biliary tract cancer cell lines (gallbladder carcinoma cell line and cholangiocarcinoma cell line) in vivo and in vitro, and to investigate the perspective of histone deacetylase inhibitor in its clinical application. METHODS: The survival rates of gallbladder carcinoma cell line (Mz-ChA-l cell line) and cholangiocarcinoma cell lines (QBC939, KMBC and OZ cell lines) treated with various doses of TSA were detected by methylthiazoy tetrazolium (MTT) assay. A nude mouse model of transplanted gallbladder carcinoma (Mz-ChA-l cell line) was successfully established, and changes in the growth of transplanted tumor after treated with TSA were measured. RESULTS: TSA could inhibit the proliferation of gallbladder carcinoma cell line (Mz-ChA-l cell line) and cholangiocarcinoma cell lines (QBC939, KMBC and OZ cell lines) in a dose-dependent manner. After the nude mouse model of transplanted gallbladder carcinoma (Mz- ChA-l cell line) was successfully established, the growth of cancer was inhibited in the model after treated with TSA. CONCLUSION: TSA can inhibit the growth of cholangiocarcinoma and gallbladder carcinoma cell lines in vitro and in vivo.

关键词： Biliary tract cancer Gallbladder carcinoma Cholangiocarcinoma Proliferation Trichostatin A

Model-driven full system dynamics estimation of PMSM-driven chain shell magazine

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DEFENCE TECHNOLOGY 2024年第9期39卷 147-156页

作者： Wei, Kai Chen, Longmiao zou, quan NanJing Univ Sci & Technol Sch Mech Engn Nanjing 210094 Peoples R China

Based on the system dynamic model, a full system dynamics estimation method is proposed for a chain shell magazine driven by a permanent magnet synchronous motor (PMSM). An adaptive extended state observer (AESO) is proposed to estimate the unmeasured states and disturbance, in which the model parameters are adjusted in real time. Theoretical analysis shows that the estimation errors of the disturbances and unmeasured states converge exponentially to zero, and the parameter estimation error can be obtained from the extended state. Then, based on the extended state of the AESO, a novel parameter estimation law is designed. Due to the convergence of AESO, the novel parameter estimation law is insensitive to controllers and excitation signal. Under persistent excitation (PE) condition, the estimated parameters will converge to a compact set around the actual parameter value. Without PE signal, the estimated parameters will converge to zero for the extended state. Simulation and experimental results show that the proposed method can accurately estimate the unmeasured states and disturbance of the chain shell magazine, and the estimated parameters will converge to the actual value without strictly continuous PE signals. (c) 2024 China Ordnance Society. Publishing services by Elsevier B.V. on behalf of KeAi Communications Co. Ltd. This is an open access article under the CC BY-NC-ND license (http://***/ licenses/by-nc-nd/4.0/).

关键词： Chain shell magazine Full system dynamics estimation Disturbance estimation Parameter estimation Adaptive extended state observer PARAMETER-ESTIMATION NONLINEAR-SYSTEMS STATE

Identification of methylation profile of HOX genes in extrahepatic cholangiocarcinoma

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World Journal of Gastroenterology 2011年第29期17卷 3407-3419页

作者： Yi Shu Bing Wang Ji Wang Jian-Ming Wang sheng-quan zou General Surgery Department of Tongji Hospital Tongji Medical College of Huazhong University of Science and Technology1095 Jiefang AvenueWuhan 430030Hubei ProvinceChina

AIM:To identify methylation profile and novel tumor marker of extrahepatic cholangiocarcinoma(CCA)with high throughout ***:Differential methylation profile was compared between normal bile duct epithelial cell lines and CCA cell lines by methyl-DNA immunoprecipitation (MeDIP)***-polymerase chain reaction (BSP)was performed to identify the methylated allels of target *** of target genes was investigated before and after the treatment with DNA demethylating *** of candidate genes was also evaluated by immunofluorescence in 30 specimens of CCA tissues and 9 normal bile duct ***:Methylation profile of CCA was identified with MeDIP microarray in the respects of different gene functions and signaling ***,97 genes with hypermethylated CpG islands in the promoter region were homeobox *** top 5 hypermethylated homeobox genes validated by BSP were HOXA2(94.29%),HOXA5(95.38%),HOXA11 (91.67%),HOXB4(90.56%)and HOXD13(94.38%).Expression of these genes was reactivated with 5’-aza2’-*** expression differences were found between normal bile duct and extrahepatic CCA tissues(66.67%-100%vs 3.33%-10%).CONCLUSION:HOXA2,HOXA5,HOXA11,HOXB4 and HOXD13 may work as differential epigenetic biomarkers between malignant and benign biliary tissues.

关键词： DNA methylation Epigenetic Promoter microarray Cholangiocarcinoma

Narrowband blue emitter based on fused nitrogen/carbonyl combination with external quantum efficiency approaching 30%

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Science China Chemistry 2024年第1期67卷 351-359页

作者： Long Chen Jia-Heng Cai You-Jun Yu Yang-Kun Qu sheng-Yi Yang sheng-Nan zou Rui-Hong Liu Dong-Ying Zhou Liang-sheng Liao Zuo-quan Jiang Institute of Functional Nano&Soft Materials(FUNSOM) Jiangsu Key Laboratory for Carbon-Based Functional Materials&DevicesSoochow UniversitySuzhou 215123China Macao Institute of Materials Science and Engineering Macao University of Science and TechnologyMacao 999078China

Multi-resonance thermally activated delayed fluorescence(MR-TADF)emitters can enable narrowband emission with high color purity and electroluminescence ***/carbonyl(N/C=O)system is receiving increasing attention while the nitrogen/boron(N/B)system has been widely *** decoration is an effective approach for N/B type MR-TADF system but always leads to broadening and red-shifting of the emission band in N/C=O MR-TADF *** attribute these unfavorable phenomena to the formation of intramolecular charge transfer between the MR-core and peripheral *** address this issue,we have developed a new strategy by decorating DMQAO(a fused N/C=O MR-core)with a triazine acceptor and a neutral terphenyl group to construct MTDMQAO and MBDMQAO,*** introduction of the triazine acceptor not only realizes efficient narrowband emission in MTDMQAO,but also accelerates the reverse intersystem crossing process through enhanced spin-orbital *** a result,MTDMQAO exhibits a significantly higher external quantum efficiency of 29.4%compared to the referent emitters,validating the rationality of our derivation *** study highlights the potential of the N/C=O system for MR-TADF emitters and provides important insights for understanding the difference between N/B and N/C=O systems.

关键词： carbonyl group narrowband acceptor decoration thermally activated delayed fluorescence OLEDs

Pathogenesis of cholangiocarcinoma in the porta hepatis and infection of hepatitis virus

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Hepatobiliary & Pancreatic Diseases International 2003年第2期2卷 601-605页

作者： Xiao-Fang Liu sheng-quan zou Fa-Zu Qiu the Department of Hepatobiliary Surgery of Affiliated Yantai Yuhuangding Hospital, Qingdao University Medical College, Yantai 264000, China Department of General Surgery of Tongji Medical Hospital, Wuhan 430030, China Department of Hepatobiliary Surgery of Affiliated Yantai Yuhuangding Hospital Qingdao University Medical College Yantai 264000 China. liu634@***

OBJECTIVE: To study the correlation between human cholangiocarcinoma in the porta hepatis and the infection of hepatitis virus. METHODS: Immunohistochemistry was used to detect HBxAg and HCV-C protein in formalin-fixed and paraffin-embedded samples taken from 68 patients with cholangiocarcinoma in the porta hepatis. The findings were reviewed against the clinical records of the patients. RESULTS: Six patients (8.8%) were positive for HBxAg and 24 (35%) for HCV-C protein, respectively. One patient was positive for both HBxAg and HCV-C protein. There were statistically differences in the extent of differentiation, invasion, lymph-node metastasis, and treatment between the patients with cholangiocarcinomas in the porta hepatis with HB(C) V infection and those without infection. CONCLUSIONS: HB(C) V infection is correlated to the development of cholangiocarcinoma in the porta hepatis. The tumor with HB(C) V infection may have a higher malignancy biologically and poorer prognosis. HBxAg and HCV-C protein may play an important role in the pathogenesis of cholangiocarcinoma in the porta hepatis.

关键词： cholangiocarcinoma porta hepatis hepatitis B virus hepatitis C-like virus

Prognostic significance of grade of malignancy based on histopathological differentiation and Ki-67 in pancreatic ductal adenocarcinoma

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Cancer Biology & Medicine 2024年第5期21卷 416-432页

作者： Yuexiang Liang Guannan sheng Yu Guo Yiping zou Hanhan Guo Zhifei Li Shaofei Chang quan Man Song Gao Jihui Hao Department of Pancreatic Cancer Tianjin Medical University Cancer Institute&HospitalNational Clinical Research Center for CancerKey Laboratory of Cancer Prevention and TherapyTianjinTianjin’s Clinical Research Center for CancerTianjin 300060China Department of Gastrointestinal Oncology The First Affiliated Hospital of Hainan Medical UniversityHaikou 570102China Department of Gastrointestinal Pancreatic Surgery Shanxi Provincial People’s HospitalTaiyuan 030012China

Objective:Tumor cell malignancy is indicated by histopathological differentiation and cell ***-67,an indicator of cellular proliferation,has been used for tumor grading and classification in breast cancer and neuroendocrine ***,its prognostic significance in pancreatic ductal adenocarcinoma(PDAC)remains ***:Patients who underwent radical pancreatectomy for PDAC were retrospectively enrolled,and relevant prognostic factors were *** of malignancy(GOM),a novel index based on histopathological differentiation and Ki-67,is proposed,and its clinical significance was ***:The optimal threshold for Ki-67 was determined to be 30%.Patients with a Ki-67 expression level>30%rather than≤30%had significantly shorter 5-year overall survival(OS)and recurrence-free survival(RFS).In multivariate analysis,both histopathological differentiation and Ki-67 were identified as independent prognostic factors for OS and *** GOM was used to independently stratify OS and RFS into 3 tiers,regardless of TNM stage and other established prognostic *** tumor-nodemetastasis-GOM stage was used to stratify survival into 5 distinct tiers,and surpassed the predictive performance of TNM stage for OS and ***:Ki-67 is a valuable prognostic indicator for *** of the GOM in the TNM staging system may potentially enhance prognostic accuracy for PDAC.

关键词： Pancreatic ductal adenocarcinoma prognosis Ki-67 differentiation TNM stage