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Effects of Different Preservation Methods on Activity of Recombinant E. coli

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Animal Husbandry and Feed Science 2009年第6期1卷 16-18页

作者： ZHOU Zhi-hui GAO bo WANG sheng-zhi cen Ning College of Animal Science and Technology Yangzhou University Yangzhou 225009 China

[ Objective] To explore different preservation methods of recombinant E. coli and find out the optimal conditions for preservation. [ Method] The recombinant E. coli DH5cx transformed pcDNA.3 were respectively preserved at 4℃ and -70 ℃, and the activity was determined after dif- ferent time. [ Result] The number of living E. coll with high dilutions preserved at 4 ℃ was gradually increased within the first 7 d, peaked on Day 7, and then gradually decreased. The number of living E. coli, which were preserved in 8% glycerol at -70℃ when OD800 at 0.8, were significantly higher than that of other groups after different preservation time. [ Conclusion] The optimal storage time was 7 d for recombinant E. coli at 4 ℃. For preservation at -70 ℃, the bacteria should be in logarithmic growth phase and preserved in 8% glycerol.

关键词： Recombinant E. coli Preservation methods Living bacteria

Prolonged and recurrent hypoglycemia induced by trimethoprim-sulfamethoxazole in a Hodgkin lymphoma patient with Pneumocystis carinii pneumonia

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Chinese Medical Journal 2021年第10期134卷 1230-1232页

作者： Bing-Jie Wang Zhi-Hao Liu Qing-Yun Wang Wei Liu bo Tang Zhi-Xiang Qiu Wen-sheng Wang Mang-Ju Wang Jin-Ping Ou Han-Yun Ren Xi-Nan cen Department of Hematology Peking University First HospitalBeijing 100034China Department of Cardiology Peking University First HospitalBeijing 100034China

To the Editor:A 64-year-old Chinese man(65 kg)with fever and lymphadenopathy was admitted to the hematology department of the Peking University First *** admission,he had had intermittent fever for the past 3 months with a body temperature high of 38.5°*** had no accompanying discomfort and his temperature sometimes dropped to normal without *** month before admission,he found several palpable enlarged cervical lymph *** admission,a lymph node biopsy was conducted and the pathological diagnosis showed a nodular sclerosis,a subtype of classical Hodgkin lymphoma.

关键词： admitted lymphoma diagnosis

Phase transition of a diblock copolymer and homopolymer hybrid system induced by different properties of nanorods

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Chinese Physics B 2018年第5期27卷 526-533页

作者： Xiao-bo Geng Jun-xing Pan Jin-jun Zhang Min-na Sun Jian-yong cen School of Physics and Information Engineering Shanxi Normal UniversityLinfen 041004China School of Chemistry and Materials Science Shanxi Normal UniversityLinfen 041004China

We investigated phase transitions in a diblock copolymer–homopolymer hybrid system blended with nanorods(NRs)by using the time-dependent Ginzburg–Landau *** systematically studied the effects of the number,length and infiltration properties of the NRs on the self-assembly of the composites and the phase transitions occurring in the *** analysis of the phase diagram was carried out to obtain the formation conditions of sea island structure nanorodbased aggregate,sea island structure nanorod-based dispersion,lamellar structure nanorod-based multilayer arrangement and nanowire *** analysis of the evolution of the domain sizes and the distribution of the nanorod angle microphase structure was *** simulation provides theoretical guidance for the preparation of ordered nanowire structures and a reference to improve the function of a polymer nanocomposite material.

关键词： self-assembly block copolymer nanowires

Blood typing and transfusion therapy in a patient with A2 subtype acute myeloid leukemia M2:A case report

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World Journal of Clinical Cases 2023年第16期11卷 3813-3821页

作者： Xiao-Chuan Kuang Shi-Hua Zhang Yi-Jing cen Jian-bo Zhang Yu-Song Liu Department of Clinical Laboratory Sichuan Provincial People's HospitalSichuan Academy of Medical SciencesChengdu 610000Sichuan ProvinceChina Department of Gastroenterology Pidu District People's HospitalChengdu 610000Sichuan ProvinceChina

BACKGROUND Acute myeloid leukemia(AML)is one of the most common types of leukemia in ***,AML is relatively rare in the population overall,accounting for only about 1 percent of all *** for AML can be very effective for some patients,yet it leaves others with serious and even life-threatening side *** is still the primary treatment for most AML,but over time,leukemia cells become resistant to chemotherapy *** addition,stem cell transplantation,targeted therapy,and immunotherapy are currently *** the same time,with the progression of the disease,the patient may have corresponding complications,such as coagulation dysfunction,anemia,granulocytopenia,and repeated infection,so transfusion supportive therapy will be involved in the overall treatment *** date,few articles have reported on blood transfusion treatment options for patients with Abo subtypes *** transfusion therapy is an important supportive treatment for AML-M2,and accurate determination of patients'blood type is one of the most important steps in the treatment *** this study,we explored blood typing and supportive treatment strategies for a patient with A2 subtype AML-M2 to provide the basis for treatment for all *** SUMMARY In order to determine the blood type of the patient,serological and molecular biological methods were used for reference tests,and the genetic background was studied to determine the patient's final blood type and select the appropriate blood products for infusion *** to the results obtained by serological and molecular biological methods,the blood type of the patient was A2 subtype;the genotype was A02/001;the irregular antibody screening was negative,and anti-A1 was found in the *** to the overall treatment plan,active anti-infection,elevated cells,component blood transfusion support,and other rescue and supportive treatments were given,and the patient successfully passe

关键词： Abo blood-group system A2 subtypes Blood grouping and crossmatching Blood transfusion Acute myeloid leukemia Atypical blood transfusion

Clinical characteristics and outcomes of primary intracranial alveolar soft-part sarcoma: A case report

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World Journal of Clinical Cases 2022年第1期10卷 296-303页

作者： Jun-Yu Chen bo cen Fei Hu Yong Qiu Guo-Min Xiao Jun-Ge Zhou Fang-Cheng Zhang Department of Neurosurgery General Hospital of the Yangtze River ShippingWuhan 430010Hubei ProvinceChina Department of Neurosurgery Wuhan Union HospitalWuhan 430010Hubei ProvinceChina

BACKGROUND Primary intracranial alveolar soft-part sarcoma(PIASPS)is a rare *** aimed to investigate the clinical profiles and outcomes for *** SUMMARY We firstly reported five consecutive cases from our ***,the cases from previous studies were pooled and analyzed to delineate the characteristics of this *** cohort included two males and three *** median age was 21-years-old(range:8-54-years-old).All the patients received surgical *** total resection(GTR),radiotherapy,and chemotherapy were administered in 3 patients,4 patients,and 1 patient,*** a median follow-up of 36 mo,tumor progression was noticed in 4 patients;and 3 patients died of the *** data(n=14)contained 5 males and 9 females with a median age of 19 *** log-rank tests showed that GTR(P=0.011)could prolong progression-free survival,and radiotherapy(P<0.001)resulted in longer overall *** Patients with PIASPS suffer from poor *** treatment is the first choice,and GTR should be achieved when the tumor is *** with PIASPS benefit from radiotherapy,which should be considered as a part of treatment therapies.

关键词： Primary intracranial alveolar soft-part sarcoma Surgery Radiotherapy Progression-free survival Overall survival Case report

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带式输送系统节能优化调度研究

制造业自动化 2024年第5期46卷 114-119页

作者：岑梁尹小明黄海棠胡文博张世荣国网浙江长兴县供电有限公司浙江湖州313100 国网电力科学研究院武汉能效测评有限公司湖北武汉430074 武汉大学电气与自动化学院湖北武汉430072

带式输送系统具有运输量大、单位耗能低等优点,在水泥熟料输送中获得了应用。以带速和运输量控制为手段,研究水泥熟料带式输送系统的优化调度策略,旨在提升系统能源效率并抑制带速波动;还深入研究了优化时域宽度对调度策略性能的影响。... 详细信息

带式输送系统具有运输量大、单位耗能低等优点,在水泥熟料输送中获得了应用。以带速和运输量控制为手段,研究水泥熟料带式输送系统的优化调度策略,旨在提升系统能源效率并抑制带速波动;还深入研究了优化时域宽度对调度策略性能的影响。选取某水泥熟料带式输送系统为例,分别在入库量波动、满负荷及低负荷3种工况下对优化调度策略进行了研究,结果表明:优化调度策略在3种工况下均取得了可观的节费率和节能率;当预测宽度大于等于4h,提出的调度策略即可获得较大收益,有效缩短了预测宽度。

关键词：分时电价优化调度带式输送机能耗模型水泥熟料

Structure and Luminescent Property of a Tetrahedral Silver(Ⅰ) Cluster Complex Based on Phosphor and Sulphur Mixed-ligand System

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Chinese Journal of Structural Chemistry 2019年第7期38卷 1216-1222页

作者： WANG Yue SHI Yong-Fang LI Xiao-bo ZOU Xiao-Chuan HE Yu-cen WANG Xia College of Biological and Chemical Engineering Chongqing University of Education Chongqing 400067 China Fujian Institute of Research on the Structure of Matter State Key Laboratory of Structural Chemistry Chinese Academy of Sciences Fuzhou 350002 China

A tetrahedral silver(I) cluster complex {Ag4[HC(PPh2)3](S2PPh2)3}ClO4(1,HC(PPh2)3 = 1,1,1-tris(triphenylphosphino)methane, S2PPh2 = diphenyldithiophosphinate) was synthesized by assembling silver ion with phosphor and sulphur mixed-ligand system. The complex was characterized by single-crystal X-ray crystallography, high-resolution mass spectroscopy and thermal analysis. In the crystal structure, an inverted tetrahedron was constructed from Ag4 metal skeleton. The Ag4 cage was stabilized by three diphenyldithiophosphinate ligands and one 1,1,1-tris(triphenylphosphino)methane ligand. 1 exhibits luminescent properties in solid state. A bright yellow-green emission centered at 533 nm has been observed in solid state. Large Stokes' shifts with long range of emissive lifetime indicate that the emission likely originates from a triplet state parentage.

关键词： silver cluster crystal structure luminescent property mixed-ligand

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堆石坝混凝土面板施工期裂缝成因及预防方法

应用基础与工程科学学报 2023年第4期31卷 811-827页

作者：袁敏强晟岑威钧胡勇陈波河海大学水利水电学院江苏南京210098 浙江省水利水电勘测设计院浙江杭州310002

针对堆石坝混凝土面板施工期普遍存在开裂的问题,在前人以温度应力为主要研究对象的基础上,考虑了干缩应力,编制了温湿耦合程序并进行验证.从混凝土面板长度,面板两侧横缝约束强度,环境相对湿度和保温保湿措施方面对某堆石坝混凝土面板... 详细信息

针对堆石坝混凝土面板施工期普遍存在开裂的问题,在前人以温度应力为主要研究对象的基础上,考虑了干缩应力,编制了温湿耦合程序并进行验证.从混凝土面板长度,面板两侧横缝约束强度,环境相对湿度和保温保湿措施方面对某堆石坝混凝土面板进行了温度应力、干缩应力以及温湿耦合应力仿真计算,并对计算结果的温度、湿度、应力进行了综合的对比与分析.结果表明,当环境相对湿度为0.3,温湿耦合应力最大值是温度应力最大值的近2.46倍.当环境相对湿度为0.6,相对湿度下降相同的幅度5%,第60天拆模拉应力涨幅为第5天拆模拉应力涨幅的近2.28倍.此外,横缝约束作用将导致面板最大拉应力最大涨幅约为75%.解释了该堆石坝混凝土面板施工期裂缝成因,得出了“半定量”的关系,并提出相关的预防方法,可为类似工程提供参考.

关键词：堆石坝混凝土面板开裂温度应力干缩应力温湿耦合横缝约束

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常见干字型输电塔地震易损性分析

华北地震科学 2024年第3期42卷 11-16页

作者：陈刚王郁李茂峰刘波陈强唐勇岑关李小军荣棉水中国南方电网有限责任公司超高压输电公司百色局广西百色533000 北京工业大学城市与工程安全减灾教育部重点实验室北京100124

为了研究输电塔-线体系中输电塔破坏的问题,根据某实际输电塔进行有限元建模,基于分析结果确定并定义损伤状态极限,选择20条地震动记录进行调幅,使用增量动力分析方法,以输电塔主材和斜材的杆件节间相对位移角为地震需求参数,地震动峰... 详细信息

为了研究输电塔-线体系中输电塔破坏的问题,根据某实际输电塔进行有限元建模,基于分析结果确定并定义损伤状态极限,选择20条地震动记录进行调幅,使用增量动力分析方法,以输电塔主材和斜材的杆件节间相对位移角为地震需求参数,地震动峰值加速度为强度参数,通过IDA分析方法得到输电塔的易损性曲线,对输电塔进行震害分析。通过体系中输电塔的易损性曲线可以与输电塔单塔的易损性曲线进行对比,得到塔-线体系耦连对输电塔的影响。

关键词：实际输电塔震害增量动力分析地震易损性