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Lipopolysaccharide induced synthesis of CD14 protein and its gene expression in hepatocytes during endotoxemia

World Journal of Gastroenterology 2002年第1期8卷 124-127页

作者： sheng-weili Jian-PingGong 等 DepartmentofGeneralSurgery TheSecondCollegeofClinicalMedicine&TheSecondAffiliatedHospitalofChongqingUniversityofMedicalSciencesChongqing400010China DepartmentofGeneralSurgery TheSecondCollegeofClini

AIM:To observe synthesis of CD14 protein and expressionof CD14 mRNA in hepatic tissue and hepatocytes of ratsduring ***:The endotoxemia model of Wistar rat wasestablished by injection of a dose of lipopolysaccharide(LPS)(5mg·kg^(-1),Escherichia coil O111:B4)via the tailvein,then the rats were sacrificed after 3,6,12 and 24 h *** were isolated from normal and LPS-injected rats by in situ collagenase perfusion technique andwere collected to measure the expression of CD14 mRNAand synthesis of CD14 protein by reverse transcription-polymerase chain reaction(RT-PCR)or Western *** binding of fluorescein isothiecyanate(FITC)-CD14 polyclonal antibody to isolated hepatocytes was alsoassessed by flow cytometric analysis(FCM).RESULTS:In the rats with endotoxemia,the expressions ofCD14 mRNA in hepatic tissue and isolated hepatocytes werestronger at 3,6,and 12 h than that in control rats(3.48±0.15,5.89±0.62,4.33±0.18,vs 1.35±0.14 in hepatictissue,P<0.01;4.12±0.17,6.24±0.64,4.35±0.18,vs1.87±0.15 in hepatecytss,P<0.01).The synthesis of CD14protein in hepatic tissue and isolated hepatoeytes increasesalso obviously in 6 and 12 h when compared to that incontrol rats(13.27±1.27,17.32±1.35,11.42±1.20,vs 7.34±0.72 in hepatic tissue,P<0.01;14.68±_+1.30,17.95±1.34,11.65±1.19,vs 7.91±0.70 in hepatocytoes,P<0.01).FCM showed that mean fluorescence intensity(MFI)andnumbers of FITC-CD14 positive cells in the rats withendotoxemia increased obviously at 3,6,12 and 24h whencompared with normal control group(43.4%,70.2%,91.4%,32.6% vs4.5%,P<0.01).CONCLUSION:LPS can markedly promote the synthesis ofCD]4 protein and up-regulate the expression of CD14 mRNAin isolated hepatocytes and hepatic *** might be amain source for soluble CD14 production duringendotoxemia.

关键词：内毒素血症脂多糖 CD14蛋白诱导合成基因表达肝细胞动物实验

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Role of NF-kB in multiple organ dysfunction during acute obstructive cholangitis

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World Journal of Gastroenterology 2003年第1期9卷 179-183页

作者： BinTu Jian-PingGong Hu-YiFeng Chuan-XinWu Yu-JunShi Xu-HongLi YongPeng Chang-AnLiu sheng-weili DepartmentofGeneralSurgery theSecondCollegeofClinicalMedicine&theSecondAffiliatedHospitalofChongqingUniversity'ofMedicalScience74LinjiangRoadChongqing400010China DepartmentofGeneralSurgery theSecondCollegeofClinicalMedicine&theSecondAffiliatedHospitalofChongqingUniversity'ofMedicalScience74LinjiangRoadChongqing400010China

AIM:To elucidate the role of NF-kB activation in the development of multiple organ dysfunction(MOD)during acute obstructive cholangitis(AOC)in rats. METHODS:Forty-two Wistar rats were divided into three groups:the AOC group,the group of bile duct ligation(BDL group),and the sham operation group(SO group).All the animals in the three groups were killed in the 6th and 48th hour after *** changes of vital organs were observed under light and electron ***-κB activation was determined with Electrophoretic Mobility Shift Assay(EMSA).Arterial blood gas analyses and the serum levels of lactate dehydrogenase(LDH),alanine aminotransferase(ALT),blood urea nitrogen(BUN)and creatinine were *** concentrations of TNF-α and IL-6 in plasma were also measured. RESULTS:The significant changes of histology and ultrastructure of vital organs were observed in AOC group. By contrast,in BDL group,all the features of organs damage were greatly *** of NF-κB activation in various tissues increased in AOC group when compared to other two *** 6 h,the arterial pH in three groups was 7.52±0.01,7.46±0.02,and 7.45±0.02,and the blood pCO_2 was 33.9±0.95 mmHg,38.1±0.89 mmHg,38.9±0.94 mmHg,there was difference in three groups(P<0.05).At 48 h,the blood pH values in three groups was 7.33±0.07, 7.67±0.04,and 7.46±0.03,and blood HCO_3^- was 20.1±1.29 mmol·L^(-1),26.7±1.45 mmol·L^(-1)and 27.4±0.35 mmol·L^(-1),there was also difference in three groups(P<0.05).In AOC group, Levels of LDH,ALT,BUN and creatinine were 16359.9±2278.8 nkat·L^(-1),5796.2±941.9 nkat·L^(-1),55.7±15.3 mg/dl,and 0.72± 0.06 mg/dl,which were higher than in SO group(3739.1± 570.1 nkat·L^(-1),288.4±71.7 nkat·L^(-1),12.5±2.14 mg/dl,and 0.47±0.03 mg/dl)(P<0.05).Levels of plasma TNF-α and IL-6 in AOC at 48 h were 429±56.62 ng·L^(-1)and 562±57 ng·L^(-1), which increased greatly when compared to BDL group (139±16 ng·L^(-1),227±43 ng·L^(-1))and SO group(74±10 ng·L^(-1), 113±19 ng·L^(-

关键词：核因子-κB 急性阻塞性胆管炎多器官功能失调病理机制动物模型

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Synthesis of endotoxin receptor CD14 protein in Kupffer cells and its role in alcohol-induced liver disease

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World Journal of Gastroenterology 2003年第3期9卷 622-626页

作者： Li-LiDai Jian-PingGong Guo-QingZuo Chuan-XinWu Yu-JunShi Xu-HongLi YongPeng WuDeng sheng-weili chang-AnLiu DepartmentofDigestiveDisease theSecondCollegeofClinicalMedicine&theSecondAffiliatedHospitalofChongqingUniversityofMedicalScience74LinjiangRoadChongqing400010China DepartmentofGeneralSurgery theSecondCollegeofClinicalMedicine&theSecondAffiliatedHospitalofChongqingUniversityofMedicalScience74LinjiangRoadChongqing400010China

AIM: To observe the synthesis of endotoxin receptor CD14 protein and its mRNA expression in Kupffer cells (KCs), and evaluate the role of CD14 in the pathogenesis of liver injury in rats with alcohol-induced liver disease (ALD).METHODS: Twenty-eight Wistar rats were divided into two groups: ethanol-fed group and control group. Ethanol-fed group dextrose instead of ethanol. Two groups were sacrificed at 4 wk and 8 wk, respectively. KCs were isolated and the synthesis of CD14 protein and its mRNA expression in KCs were determined by flow cytometric analysis (FCM)or the reverse transcription polymerase chain reaction (RTPCR) analysis. The levels of plasma endotoxin and alanine transaminase (ALT) were measured by Limulus Amebocyte Lysate assay and standard enzymatic procedures respectively, and the levels of plasma tumor necosis factor (TNF)-α and interleukin (IL)-6 were both determined by ELISA. The liver pathology change was observed under light and electric ***: In ethanol-fed group, the percentages of FITCCD14 positive cells were 76.23 % and 89.42 % at 4 wk and 8 wk, respectively. Compared with control group (4.45 %and 5.38 %), the difference was significant (P＜0.05). The expressions of CD14 mRNA were 7.56±1.02 and 8.74±1.37 at 4 wk and 8 wk, respectively, which were significantly higher compared with the control group (1.77±0.21 and 1.98±0.23)(P＜0.05). Plasma endotoxin levels at 4 wk and 8 wk increased dramatically in ethanol-fed rats (112±15 IU/L and 147±22 IU/L) than those in the control animals (31±12 IU/L and 33±9 IU/L) (P＜0.05). In ethanol-fed rats, the levels of wk, respectively which were significantly higher than those fed rats, there were marked pathological changes including steatosis, cell infiltration and necrosis. No marked pathological changes were seen in control ***: Ethanol administration led to a significantsynthesis of endotoxin receptor CD14 protein and its gene expression in KCs, which maybe result in the pathologica

关键词：内毒素受体 CD14蛋白 Kupffer细胞酒精性肝病肝功能发病机制

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教学策略在小学数学游戏课中的应用

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现代基础教育研究 2015年第2期18卷 172-177页

作者：盛玮莉上海市长宁区绿苑小学

数学游戏课不是将知识直接呈现给学生,而是让学生通过整堂课玩游戏来领悟知识、锻炼学生的思维能力,是以数学游戏为载体、以学生的感悟为基本学习手段的一种课堂教学模式。数学游戏课能使学生获得不同层次的感悟,在游戏过程中有所体验... 详细信息

数学游戏课不是将知识直接呈现给学生,而是让学生通过整堂课玩游戏来领悟知识、锻炼学生的思维能力,是以数学游戏为载体、以学生的感悟为基本学习手段的一种课堂教学模式。数学游戏课能使学生获得不同层次的感悟,在游戏过程中有所体验从而促进数学教学。经过实践研究、不断提升教法方法,教师摸索出数学游戏课教学的教学策略四步骤——激发兴趣→体验感悟→合作学习→交流分享,从促进数学游戏课的有效教学。

关键词：数学游戏课教学策略应用

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矮塔斜拉桥拉索设计及施工关键技术研究

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广东建材 2024年第2期40卷 124-127页

作者：盛维礼中铁十二局集团第一工程有限公司

斜拉索是斜拉桥的重要组成部分和重点关注对象,其设计及施工质量关系到桥梁后期服役的安全及寿命。以安徽淮-宿-蚌城际铁路淮河特大桥(124+248+124)m矮塔斜拉桥为工程背景,相继阐述了斜拉索的构造、HDPE管焊接及安装、单根拉索安装及张... 详细信息

斜拉索是斜拉桥的重要组成部分和重点关注对象,其设计及施工质量关系到桥梁后期服役的安全及寿命。以安徽淮-宿-蚌城际铁路淮河特大桥(124+248+124)m矮塔斜拉桥为工程背景,相继阐述了斜拉索的构造、HDPE管焊接及安装、单根拉索安装及张拉、索力调整和斜拉索防护等内容,并对斜拉索设计和施工过程中的关键技术进行了探讨与分析,可为类似工况斜拉桥的设计和施工提供借鉴和参考。

关键词：斜拉桥拉索设计单根张拉关键技术

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探究租赁公寓围护结构热工性能及未来政策建议

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绿色建筑 2022年第2期14卷 52-55页

作者：盛未力英格卡购物中心(中国)管理有限公司上海200235

为更清楚租赁公寓围护结构的保温隔热措施的性质,选取某实际公寓进行研究。通过对其进行多种方法计算,并验证了租赁公寓围护结构的实际热工性能,得出其热工性能符合相应国家标准。随着未来可能的气候变化,需继续探索相关政策的指导方向。

关键词：租赁公寓节能减排建筑能耗保温隔热围护结构热工性能

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The chemical and size distribution characteristics during one new particle formation process in Beijing

The chemical and size distribution characteristics during on...

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第十届全国气溶胶会议暨第六届海峡两岸气溶胶技术研讨会

作者： Zhang Yangmei Sun Junying Lin weili sheng Xiaojing Key Laboratory for Atmospheric Chemistry,Centre for Atmosphere Watch and Services,Chinese Academy of Meteorological Sciences,China Meteorological Administration

The aerosol mass spectrometers（AMS） and Scanning Mobility particle size（SMPS） were deployed to research the new particle formation events in *** whole campaign covers from June 5 to June 30 in ***,two representative new particle formation events were observed throughout the experiment *** features of new particle formation（NPF） process are concluded from this *** the beginning of NPF,it is shown that the new particle formation event always occur when the wind direction shift from north-west to south,and the mass concentration of aerosol is very ***,Number concentration of ultra-fine particles（10-25nm） increasing ***,increasing concentration of the reactive gases SO2,NOx were monitored just before the new particle *** O3 concentration increases coincide with the NPF *** gas-particle transformation process,sulfate,ammonium and organics particles *** variation of aerosol chemical components and size distribution during the new particle formation process shows that sulfate,ammonium and organics is the main components *** organics mass fragment analysis result proves again that it is the secondary organics not primary organics aerosol involves into the new particle formation events.

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滋阴清火汤联合超声雾化吸入治疗慢性喉炎疗效及对肺通气功能和气道炎症指标的影响

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四川中医 2022年第10期40卷 185-187页

作者：朱丽盛伟利何宁北京市大兴区人民医院呼吸科北京102627

目的:观察滋阴清火汤联合超声雾化吸入治疗慢性喉炎疗效及对肺通气功能、气道炎症指标的影响。方法:选取2019年2月~2020年11月间本院收治的92例慢性喉炎患者为研究对象,随机分为观察组和对照组,每组46例。对照组给予超神雾化吸入治疗,... 详细信息

目的:观察滋阴清火汤联合超声雾化吸入治疗慢性喉炎疗效及对肺通气功能、气道炎症指标的影响。方法:选取2019年2月~2020年11月间本院收治的92例慢性喉炎患者为研究对象,随机分为观察组和对照组,每组46例。对照组给予超神雾化吸入治疗,观察组在此基础上联合滋阴清火汤治疗。比较两组疗效、治疗前后中医证候积分、通气功能、气道炎症指标[白细胞介素-2(IL-2)、白细胞介素-6(IL-6)、C反应蛋白(CRP)、肿瘤坏死因子α(TNF-α)]和治疗期间不良反应发生率。结果:治疗后,观察组治疗有效率高于对照组(P0.05)。结论:化滋阴清火汤联合超声雾化吸入治疗CL疗效显著,有效改善CL患者临床症状,肺部通气功能得到提升,改善气道炎症情况,安全性较高。

关键词：滋阴清火汤超声雾化慢性喉炎肺通气功能气道炎症

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宏基因组二代测序辅助诊断鹦鹉热衣原体肺炎临床特征分析

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国际呼吸杂志 2022年第18期42卷 1392-1397页

作者：薛甜盛伟利王玉玲首都医科大学大兴教学医院呼吸与危重症医学科北京102600

目的分析鹦鹉热衣原体肺炎的临床表现及辅助检查特征,旨在提高对该病的认识及诊疗水平。方法收集首都医科大学大兴教学医院2021年5月至2022年3月基于宏基因组二代测序(mNGS)诊断的鹦鹉热衣原体肺炎病例8例,分析其临床表现、实验室检查... 详细信息

目的分析鹦鹉热衣原体肺炎的临床表现及辅助检查特征,旨在提高对该病的认识及诊疗水平。方法收集首都医科大学大兴教学医院2021年5月至2022年3月基于宏基因组二代测序(mNGS)诊断的鹦鹉热衣原体肺炎病例8例,分析其临床表现、实验室检查、影像学表现及治疗经过。结果8例患者中,男3例,女5例,中位年龄55岁,年龄范围为42~76岁。7例有禽类接触史,主要表现为高热(8/8)、咳嗽(7/8)、头痛(5/8)、呼吸困难(4/8)。白细胞计数大多正常(6/8),C反应蛋白升高(8/8),肝酶、心肌酶升高(5/8),血钠降低(4/8),凝血功能异常(8/8)。8例患者胸部CT均表现为大片斑片实变影,单肺病变(7/8),右侧为主,2例伴胸腔积液。诊断后给予四环素类、大环内酯类或喹诺酮类药物治疗,7例好转出院,1例死亡。结论鹦鹉热衣原体肺炎通常表现为高热、咳嗽伴肺部斑片实变影,对临床可疑病例应加强询问流行病学史,尽早行mNGS检测明确病原体,及时针对性抗感染治疗。

关键词：肺炎鹦鹉热衣原体宏基因组二代测序

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Differential distribution of PINK1 and Parkin in the primate brain implies distinct roles

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Neural Regeneration Research 2025年第4期20卷 1124-1134页

作者： Yanting Liu Wei Huang Jiayi Wen Xin Xiong Ting Xu Qi Wang Xiusheng Chen Xianxian Zhao Shihua Li Xiaojiang Li weili Yang Guangdong Key Laboratory of Non-human Primate Research Key Laboratory of CNS Regeneration(Ministry of Education)Guangdong-Hongkong-Macao CNS Regeneration Institute of Jinan UniversityJinan UniversityGuangzhouGuangdong ProvinceChina

The vast majority of in vitro studies have demonstrated that PINK1 phosphorylates Parkin to work together in mitophagy to protect against neuronal ***,it remains largely unclear how PINK1 and Parkin are expressed in mammalian *** has been difficult to address because of the intrinsically low levels of PINK1 and undetectable levels of phosphorylated Parkin in small *** this issue is critical for elucidating the in vivo roles of PINK1 and ***,we showed that the PINK1 kinase is selectively expressed as a truncated form(PINK1–55)in the primate *** the present study,we used multiple antibodies,including our recently developed monoclonal anti-PINK1,to validate the selective expression of PINK1 in the primate *** found that PINK1 was stably expressed in the monkey brain at postnatal and adulthood stages,which is consistent with the findings that depleting PINK1 can cause neuronal loss in developing and adult monkey ***1 was enriched in the membrane-bound fractionations,whereas Parkin was soluble with a distinguishable *** double staining experiments showed that PINK1 and Parkin did not colocalize under physiological conditions in cultured monkey astrocytes,though they did colocalize on mitochondria when the cells were exposed to mitochondrial *** findings suggest that PINK1 and Parkin may have distinct roles beyond their well-known function in mitophagy during mitochondrial damage.

关键词： neurodegeneration Parkin Parkinson’s disease PINK1 subcellular distribution

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