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Specialized Pro-resolving Mediators Regulate Alveolar Fluid Clearance during Acute Respiratory Distress Syndrome

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Chinese Medical Journal 2018年第8期131卷 982-989页

作者： Qian Wang Song-Fan Yan Yu Hao sheng-wei jin Department of Anesthesia and Critical Care Second Affiliated Hospital of Wenzhou Medical University Wenzhou Zhejiang 325027 China

Objective：Acute respiratory distress syndrome （ARDS） is an acute and lethal clinical syndrome that is characterized by the injury of alveolar epithelium, which impairs active fluid transport in the lung, and impedes the reabsorption of edema fluid from the alveolar space. This review aimed to discuss the role of pro-resolving mediators on the regulation of alveolar fluid clearance （AFC） in *** Sources：Articles published up to September 2017 were selected from the PubMed, with the keywords of ＂alveolar fluid clearance＂ or ＂lung edema＂ or ＂acute lung injury＂ or ＂acute respiratory distress syndrome＂ , and ＂specialized pro-resolving mediators＂ or ＂lipoxin＂ or ＂resolvin＂ or ＂protectin＂ or ＂maresin＂ or ＂alveolar epithelial cells＂ or ＂aspirin-triggered lipid mediators＂ or ＂carbon monoxide and heme oxygenase＂ or ＂annexin A1＂ .Study Selection：We included all relevant articles published up to September 2017, with no limitation of study ***：Specialized pro-resolving mediators （SPMs）, as the proinflammatory mediators, not only upregulated epithelial sodium channel, Na,K-ATPase, cystic fibrosis transmembrane conductance regulator （CFTR）, and aquaporins levels, but also improved Na,K-ATPase activity to promote AFC in ARDS. In addition to the direct effects on ion channels and pumps of the alveolar epithelium, the SPMs also inhibited the inflammatory cytokine expression and improved the alveolar epithelial cell repair to enhance the AFC in ***：The present review discusses a novel mechanism for pulmonary edema fluid reabsorption. SPMs might provide new opportunities to design ＂reabsorption-targeted＂ therapies with high degrees of precision in controlling ALI/ARDS.

关键词： Acute Lung Injury Acute Respiratory Distress Syndronle Alveolar Fluid Clearance Specialized Pro-resolving Mediator

Sepsis-induced immunosuppression:mechanisms,diagnosis and current treatment options

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Military Medical Research 2023年第2期10卷 242-261页

作者： Di Liu Si-Yuan Huang Jian-Hui Sun Hua-Cai Zhang Qing-Li Cai Chu Gao Li Li Ju Cao Fang Xu Yong Zhou Cha-Xiang Guan sheng-wei jin jin Deng Xiang-Ming Fang Jian-Xin Jiang Ling Zeng Department of Trauma Medical Center Daping HospitalState Key Laboratory of TraumaBurns and Combined InjuryArmy Medical UniversityChongqing 400042China Department of Respiratory Disease Daping HospitalArmy Medical UniversityChongqing 400042China Department of Laboratory Medicine the First Affiliated Hospital of Chongqing Medical UniversityChongqing 400016China Department of Critical Care Medicine the First Affiliated Hospital of Chongqing Medical University400016 ChongqingChina Department of Physiology School of Basic Medicine ScienceCentral South UniversityChangsha 410078China Department of Anesthesia and Critical Care the Second Affiliated Hospital and Yuying Children’s Hospital of Wenzhou Medical UniversityZhejiang 325027 WenzhouChina Department of Emergency the Affiliated Hospital of Guizhou Medical UniversityGuizhou Medical University550001 GuiyangChina Department of Anesthesiology the First Affiliated HospitalZhejiang University School of MedicineHangzhou 310052China.

Sepsis is a common complication of combat injuries and trauma,and is defined as a life-threatening organ dysfunction caused by a dysregulated host response to *** is also one of the significant causes of death and increased health care costs in modern intensive care *** use of antibiotics,fluid resuscitation,and organ support therapy have limited prognostic impact in patients with *** its pathophysiology remains elusive,immunosuppression is now recognized as one of the major causes of septic ***-induced immunosuppression is resulted from disruption of immune *** is characterized by the release of antiinflammatory cytokines,abnormal death of immune effector cells,hyperproliferation of immune suppressor cells,and expression of immune *** targeting immunosuppression,especially with immune checkpoint inhibitors,preclinical studies have demonstrated the reversal of immunocyte dysfunctions and established host ***,we comprehensively discuss recent findings on the mechanisms,regulation and biomarkers of sepsis-induced immunosuppression and highlight their implications for developing effective strategies to treat patients with septic shock.

关键词： Sepsis Immunosuppression Immune monitoring Immunomodulatory therapy

CRYSTAL AND MOLECULAR STRUCTURE OF 1-PHENYL-3-METHYL-4-( 3' -PHENYL-1', 3' -ACEDION)-PYRAZOLONE-5

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Chinese Journal of Structural Chemistry 1991年第3期 245-246页

作者： Kong Bi-Xia Yang Yan-sheng Li Yuan-Ying Chemistry Department,Zhongshan University,Guangzhou,516275 Liu Yong-sheng wei Ge-Cheng jin Zhong-sheng Changchun Institute of Applied Chemistry Aeademia Sinica,Changchun, 130022

A new 4-acetyl pyrazolone chelating agent CHNOMr=320. 348 has been synthesized. The molecular composition is different from β-Diketon and "Double β-Diketon". There are conjugated metiple bonds composed of three carbonyl groups in the molecule. A single crystal of approximate dimensions 0.1 × 0.2 × 0.5mm was mounted on an NICOLET R3M/E four-circle diffractomcter with graphite monochromated MoKa

关键词： PYRAZOLONE-5 CRYSTAL AND MOLECULAR STRUCTURE OF 1-PHENYL-3-METHYL-4 PHENYL-1 ACEDION

温度干扰下的葡萄糖水溶液近红外光谱修正方法与比较

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光谱学与光谱分析 2017年第11期37卷 3391-3398页

作者：孙翠迎韩同帅郭超盛伟楠刘瑾天津大学精密测试技术及仪器国家重点实验室天津300072

水的近红外吸收光谱受温度影响较大,采用近红外光谱法对水溶液样本进行成分测量时,温度的影响不能忽视。特别对于葡萄糖这种弱吸收成分,温度的微小变化会对其测量精度造成较大影响。实验发现葡萄糖水溶液在30℃附近时,温度每变化1摄氏度... 详细信息

水的近红外吸收光谱受温度影响较大,采用近红外光谱法对水溶液样本进行成分测量时,温度的影响不能忽视。特别对于葡萄糖这种弱吸收成分,温度的微小变化会对其测量精度造成较大影响。实验发现葡萄糖水溶液在30℃附近时,温度每变化1摄氏度,在1 160nm处溶液的吸光度变异系数约0.344 7%,这个变化约相当于500~600mg·dL^(-1)葡萄糖浓度引起的溶液吸光度变化量。将近年来发展的几种化学计量学方法用于温度扰动下葡萄糖水溶液的光谱修正,以提高葡萄糖测量精度,具体包括广义最小二乘加权法(GLSW)和外部参数正交化方法(EPO)。对不同温度的葡萄糖水溶液样品在900~1 350nm的光谱进行采集,测试了两种方法对温度变化后光谱的修正效果,考察了修正后光谱的葡萄糖预测误差,并与传统的考虑温度变量的多变量回归方法—偏最小二乘(PLS)回归法进行了比较。结果表明,GLSW和EPO方法对不同温度下的溶液光谱有好的校正效果,不同温度下溶液光谱的变异系数得到显著改善,同时两种方法在模型复杂性、温度修正效果等方面都优于传统方法。研究可推广到其他水份含量较大的样品测试场合,也对人体组织中的葡萄糖测量有借鉴价值。

关键词：近红外光谱法(NIR spectroscopy) 外部参数正交化(EPO) 广义最小二乘加权(GLSW) 葡萄糖测量温度修正

表皮生长因子受体和CD13双靶点抗体药物偶联物抑制血管生成和肿瘤细胞的迁移侵袭

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中国药学杂志 2018年第17期53卷 1457-1462页

作者：盛唯瑾弓建华江敏中国医学科学院医药生物技术研究所北京100050

目的研究表皮生长因子受体(EGFR)/CD13双靶点抗体药物偶联物Fv-LDM-NGR抑制肿瘤生长的作用机制。方法选用人乳腺癌细胞MCF-7、人肺腺癌细胞A549和人微血管内皮细胞(HMEC)-1作为研究对象。采用四甲基偶氮唑蓝(MTT)实验测定Fv-LDM-NGR对... 详细信息

目的研究表皮生长因子受体(EGFR)/CD13双靶点抗体药物偶联物Fv-LDM-NGR抑制肿瘤生长的作用机制。方法选用人乳腺癌细胞MCF-7、人肺腺癌细胞A549和人微血管内皮细胞(HMEC)-1作为研究对象。采用四甲基偶氮唑蓝(MTT)实验测定Fv-LDM-NGR对肿瘤细胞和内皮细胞增殖的影响。小管形成(tube formation)实验观察HMEC-1细胞形成的管腔结构。Transwell实验测定Fv-LDM-NGR对肿瘤细胞迁移和侵袭的影响。Western blot分析其对细胞内EGFR细胞信号通路、细胞周期信号通路以及凋亡通路的影响。利用流式细胞术、Hoechst染色和AnnexinⅤ-FITC/PI双染法观察细胞周期的变化以及细胞凋亡。结果 Fv-LDM-NGR能够显著抑制肿瘤细胞的体外增殖,其对肿瘤细胞MCF-7和A549以及人微血管内皮细胞HMEC-1的半数抑制浓度(IC50)分别为3. 27×10^(-14)、4. 72×10^(-13)和1. 17×10^(-12)mol·L^(-1)。Fv-LDM-NGR能够干扰血管内皮细胞形成管腔结构,抑制肿瘤细胞的迁移和侵袭。对细胞信号通路具有调节作用;引起细胞周期G2/M期和S期阻滞;诱导肿瘤细胞发生凋亡。结论 Fv-LDM-NGR通过干扰CD13活性,削弱肿瘤细胞的侵袭能力,阻碍血管内皮细胞形成管腔结构;通过下调EGFR的表达及磷酸化,干扰细胞信号通路,阻滞细胞周期循环和诱导细胞凋亡,抑制肿瘤细胞的增殖和迁移。

关键词：表皮生长因子受体 CD13 力达霉素抗体药物偶联物抗肿瘤

Boosting Oxygen Evolution Reaction Performance on NiFe‑Based Catalysts Through d‑Orbital Hybridization

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Nano-Micro Letters 2025年第1期17卷 281-292页

作者： Xing Wang wei Pi sheng Hu Haifeng Bao Na Yao wei Luo State Key Laboratory of New Textile Materials and Advanced Processing Technology Key Laboratory of New Textile Materials and Applications of Hubei ProvinceSchool of Materials Science and EngineeringWuhan Textile UniversityWuhan 430200People’s Republic of China College of Chemistry and Molecular Sciences Wuhan UniversityWuhan 430072People’s Republic of China

Anion-exchange membrane water electrolyzers(AEMWEs)for green hydrogen production have received intensive attention due to their feasibility of using earth-abundant NiFe-based *** introducing a third metal into NiFe-based catalysts to construct asymmetrical M-NiFe units,the d-orbital and electronic structures can be adjusted,which is an important strategy to achieve sufficient oxygen evolution reaction(OER)performance in ***,the ternary NiFeM(M:La,Mo)catalysts featured with distinct M-NiFe units and varying d-orbitals are reported in this *** and theoretical calculation results reveal that the doping of La leads to optimized hybridization between d orbital in NiFeM and 2p in oxygen,resulting in enhanced adsorption strength of oxygen intermediates,and reduced rate-determining step energy barrier,which is responsible for the enhanced OER *** critically,the obtained NiFeLa catalyst only requires 1.58 V to reach 1 A cm^(−2) in an anion exchange membrane electrolyzer and demonstrates excellent long-term stability of up to 600 h.

关键词： NiFe-based catalysts d-orbital coupling Oxygen evolution reaction Anion exchange membrane electrolyzer

The Association of Chalazal Cell Ultrastructure in Caryopsis and Grain Filling in Wheat

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Agricultural Sciences in China 2003年第5期2卷 507-512,T001,T002页

作者： ZHOU Zhu-qing, LAN sheng-yin, WANG wei-jin, ZHU Xu-tong, XU Zhen-xiu and YANG Ze-minHuazhong Agricultural University, Wuhan 430070, P. R. China Xiaogan University, Xiaogan 432100 , P. R. China Huazhong Agricultural University Wuhan 430070 P. R. China Xiaogan University Xiaogan 432100 P. R. China Huazhong Agricultural University Wuhan 430070 P. R. China

Using three wheat cultivars (Triticum aestivum L. ) , Een1, Huamai8 and 95A-10, which exhibit different grain filling characters. the relationship of ultrastructure of the chalazal cells in wheat caryop-sis and grain filling was studied. The results indicate that the lipoid and polysaccharide deposited in vacuoles of the chalazal cells have no obvious inhibitory actions to the grain filling. It appears that a direct relationship exists between the proceeding and end time of grain filling and the amount and appearing time of the lipoid, within lignin and suberin deposited on the inner wall of chalazal cells, and between grain filling rate and cultivars characteristic. The appearing time of the lipoid, within lignin and suberin deposited on the inner wall of chalazal cells in cultivar with large grain (Eenl and 95A-10) was later than that in cultivar with small grain (Huamai8), and the amount of deposits in the former was less than that in the latter. The late development of caryopsis and low rate of grain filling account for the wrinkled grain of 95A-10. The transport of assimilates in chalazal cells coexists with symplastic and apoplastic pathways, but symplastic pathway plays a major role after 24 days of flowering.

关键词： Wheat (Triticum aestivum L. ) Caryopsis Chalazal cell Grain filling ultrastructure

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碱性单细胞凝胶电泳铺胶技术的改进

复旦学报（医学版） 2004年第3期31卷 311-312页

作者：高建军丰盛梅金慰芳复旦大学放射医学研究所骨代谢研究室上海200032

目的　聚苯乙烯孔槽单层铺胶技术在碱性单细胞凝胶电泳中的可行性研究。方法　自制聚苯乙烯孔槽的载胶片 ,将 1%低熔点凝胶与淋巴细胞混合后单层铺胶 ,经 6Gyγ射线照射后进行碱性单细胞凝胶电泳 ,观察胶面和淋巴细胞的DNA损伤情况。结... 详细信息

目的　聚苯乙烯孔槽单层铺胶技术在碱性单细胞凝胶电泳中的可行性研究。方法　自制聚苯乙烯孔槽的载胶片 ,将 1%低熔点凝胶与淋巴细胞混合后单层铺胶 ,经 6Gyγ射线照射后进行碱性单细胞凝胶电泳 ,观察胶面和淋巴细胞的DNA损伤情况。结果　载胶片孔槽单层灌胶后胶面平整 ,操作过程无脱落 ;淋巴细胞DNA电泳图像清晰 ,6Gy照射产生的“彗星”形态特征明显。结论　采用聚苯乙烯孔槽进行单层铺胶的技术。

关键词：碱性单细胞凝胶电泳铺胶技术聚苯乙烯孔槽琼脂糖 γ射线照射

整合NGR肽抗EGFR单链抗体制备及其抗肿瘤活性研究

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中华肿瘤防治杂志 2014年第13期21卷 969-973页

作者：盛唯瑾商悦甄永苏中国医学科学院.北京协和医学院医药生物技术研究所北京100050

目的:利用大肠埃希菌表达基于抗表皮生长因子受体(epidermal growth factor receptor,EGFR)单链抗体和靶向CD13环状NGR肽的双靶点重组融合蛋白ER(Fv)-NGR,研究其对肿瘤细胞的亲和能力和体内外抑制作用。方法:用基因工程的方法构建重组质... 详细信息

目的:利用大肠埃希菌表达基于抗表皮生长因子受体(epidermal growth factor receptor,EGFR)单链抗体和靶向CD13环状NGR肽的双靶点重组融合蛋白ER(Fv)-NGR,研究其对肿瘤细胞的亲和能力和体内外抑制作用。方法:用基因工程的方法构建重组质粒pET-scfv-ngr,转入大肠埃希菌BL21,IPTG诱导表达带有His-tag标签肽的重组蛋白ER(Fv)-NGR,用Ni离子亲和柱进行纯化。运用细胞免疫荧光检测ER(Fv)-NGR与肿瘤细胞的结合;流式细胞术分析重组蛋白与肿瘤细胞的亲和力;克隆形成实验测定重组蛋白对肿瘤细胞体外增殖的抑制作用;利用人乳腺癌MCF-7裸鼠移植瘤模型对ER(Fv)-NGR的体内抗肿瘤活性进行研究。结果:重组蛋白ER(Fv)-NGR由抗EGFR单链抗体和3个连续的环状NGR肽构成,相对分子质量约为27×104,以包涵体形式在大肠埃希菌内表达,表达量约为5mg/L。细胞免疫荧光结果表明,重组蛋白与EGFR和CD13皆高表达MCF-7细胞有较强的结合能力,而与正常HEK293细胞结合较弱。ER(Fv)-NGR与MCF-7细胞结合的解离常数为8.4×10-7 nmol/L。ER(Fv)-NGR在体外对肿瘤细胞的增殖有抑制作用,作用于MCF-7和A549细胞的IC50值分别为1.2×10-6和4.7×10-6 mol/L。在体内对人乳腺癌MCF-7裸鼠移植瘤有生长抑制作用,在10mg/kg药物剂量下28d对照组与实验组瘤体积分别为(1.06±0.17)和(0.63±0.11)cm3,F=18.7,P=0.003;瘤质量分别为(0.95±0.12)和(0.52±0.07)g,F=42.7,P<0.001;抑瘤率为45.4%。结论:成功制备了基于单链抗体和靶向肽的双靶点融合蛋白ER(Fv)-NGR,其对肿瘤细胞具有良好的亲和力和体外增殖抑制作用,能够有效抑制裸鼠移植瘤的生长,为研制新型双靶点抗肿瘤药物提供依据。

关键词：肿瘤单链抗体靶向肽表皮生长因子受体 CD13 流式细胞术