检索结果-南通市图书馆

Coimmunization with IL-15 plasmid enhances the longevity of CD8 T cells induced by DNA encoding hepatitis B virus core antigen

引用

World Journal of Gastroenterology 2006年第29期12卷 4727-4735页

作者： Wei Zhang sheng-fu dong Shu-Hui Sun Yuan Wang Guang-Di Li Di Qu Institute of Molecular Medicine and Genetics Medical College of Georgia Augusta Georgia 30912 United States Key laboratory of Medical Molecular Virology Shanghai Medical College of Fudan University Shanghai 200032 China State Key Laboratory of Molecular Biology Institute of Biochemistry and Cell Biology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Shanghai 200031 China

AIM： To test the feasibility of delivering a plasmid encoding IL-15 as a DNA vaccine adjuvant for improving the immune responses induced by hepatitis B virus core gene DNA vaccine. METHODS： We used RT-PCR based strategies to develop IL-15 expression constructs. We first confirmed that the gene could be expressed in Escherichia coli due to the poor expression of IL-15. Then the bioactivity of IL-15 plasmid expression product was identified by CTLL-2 proliferation assay. One hundred micrograms of DNA from each of the IL-15 eukaryotic expressed plasmid and the recombinant plasmid harboring DNA encoding the 144 amino acids of the N-terminus of HBV core gene （abbreviated pHBc144） was used to co-immunize C57 BL/6 mice. The titer of anti-HBcIgG was detected by ELISA and the antigen-specific CD8^＋T cells （CD8^＋IFN-γ^＋ T cells） were detected by intracellular cytokine staining at different time points. RESULTS： After co-immunization by pIL-15 and pHBc144 DNA vaccine the antigen-specific CD8^＋ cells of mice increased gradually, the first peak of immune response appeared 14 d later, then the number of antigen-specific CD8^＋Ts cells decreased gradually and maintained at a steady level in 3 mo. After boosting, the number of antigen-specific CD8^＋T cells reached the second peak 10 d later with a double of the 1st peak, then the number of antigen-specific CD8^＋T cells decreased slowly. IL-15 as a gene adjuvant had no significant effect on humoral immune responses induced by hepatitis B virus core gene DNA vaccine, but increased the memory antigen-specific CD8^＋T cells induced by hepatitis B virus core gene DNA vaccine. CONCLUSION： DNA vaccine constructed by HBc Ag 1-144 amino acid induces effective cell immunity, and cytokine plasmid-delivered IL-15 enhances the longevity of CD8^＋ T cells.

关键词： Vaccine DNA vaccine Hepatitis B virus coreantigen

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Determination of the Number of SSR Alleles Necessary for the Analysis of Genetic Relationships Between Maize Inbred Lines

引用

Agricultural Sciences in China 2010年第12期9卷 1713-1725页

作者： WU Cheng-lai, LI sheng-fu, dong Bing-xue, ZHANG Qian-qian and ZHANG Chun-qing State Key Laboratory of Crop Biology/College of Agriculture, Shandong Agricultural University, Tai’an 271018, P.R.China State Key Laboratory of Crop Biology/College of Agriculture Shandong Agricultural University Tai'an 271018 P.R.China

The amount of molecular marker information has considerable impact on the results of studies of crop germplasm genetic relationships in crop. The number of alleles required to reveal genetic relationship in maize inbred lines is a theoretical issue that needs to be addressed. In this study, 112 pairs of SSR （simple sequence repeat） primers and 97 maize inbred lines were selected to study the relationship between the number of inbred lines and the number of SSR primers and alleles required for a stable cluster. The results showed that the number of SSR primers is not tightly associated with the stability of the cluster analysis results, while an increase in the number of alleles can significantly improve the stability of cluster analysis results. The number of inbred lines （X） is significantly associated with the number of alleles required for stable cluster analysis （Y）, and the regression equation is Y- 600.8xe（-15.9/x）. This equation can be used to calculate the number of SSR alleles required for a genetic relationship study of maize inbred lines. These results provide a reference for determining of SSR alleles number in genetic relationship analysis of maize inbred line and other crop germplasm.

关键词： maize inbred line SSR number of alleles genetic relationship

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Positive effect of Mo on mechanical properties of a Ni-42W-10Co-x Mo medium heavy alloy

引用

China Foundry 2025年

作者： Guo-xin Cao Jian-xin dong Yong-sheng He He Jiang Fang Hao Zhi-hua Nie Teng-fei Ma Bao-quan fu School of Materials Science and Engineering University of Science and Technology Beijing Xi'an Superalloy Technology Co. Ltd. School of Materials Science and Engineering Beijing Institute of Technology Key Laboratory of Air-driven Equipment Technology of Zhejiang Province Quzhou University

In this study,a novel Ni-W-Co-Mo medium heavy alloy (MHA) was designed to improve its mechanical strength via Mo *** the Ni-42W-10Co-xM o alloy series,where x represents the weight percent of Mo and varies between 0,1,2,5,and 10,the microstructure transitions from a dendritic structure to a hypoeutectic structure as the Mo content increases from 0 to 5wt.%.Moreover,as the Mo content increases from 0to 10wt.%,the distribution of theμ-phase shifts from being individually dispersed to forming aggregates,and its volume fraction rises from 0.5%to 7.9%.Notably,theμ-phase evolves into an eutectic microstructure,which helps in minimizing the segregation of *** change is accompanied by a substantial enhancement in mechanical properties;specifically,the compressive yield strength at room temperature increases from 350 MPa to 646 MPa,indicating a significant 85%***,the microhardness increases from 230 HV to 304 *** dynamics simulations further reveal that the strengthening mechanism of Ni-42W-10Co-xM o alloys is Mo-induced solid solution strengthening and precipitation strengthening.

关键词：

来源：

同方期刊数据库评论

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Evaluation on Genetic Diversity of Hybrid Parents Developed in Brassic napus

引用

Agricultural Sciences in China 2003年第9期2卷 1054-1060页

作者： LIU Ping wu, ZHOU Guo ling, YANG Guang sheng and fu Ting dong (National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University/ Research Center of National Rapeseed Improvement, Wuhan 430070, P.R.China) National Key Laboratory of Crop Genetic Improvement Huazhong Agricultural University/Research Center of National Rapeseed Improvement Wuhan 430070 P.R.China

Genetic diversity of 50 Brassic napus varieties including 21 sterile lines, 16 restorer lines and 13 check cultivars was estimated by SSR and ISSR. The UPGMA results showed that 50 Brassica napus varieties were divided into 4 groups. The restorer lines tested were classified into groupsⅠⅢ respectively. The sterile lines tested were classified into groupⅣ. In addition, the sterile lines could be further divided into two subgroups: except 1471AB, all genic male sterile lines were in subgroupⅠ, 1471AB and all cytoplasmic male sterile lines were in the subgroupⅡ. The UPGMA results also indicated that the restorer lines had more genetic diversity than the sterile lines.

关键词： Brassic napus Hybrid parents Genetic diversity SSR ISSR

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

我国西部地区个人现金卫生支出致贫影响研究——基于少数民族边远地区的调查

引用

中国卫生政策研究 2017年第6期10卷 68-72页

作者：陈鸣声钱东福南京医科大学医政学院江苏南京211166

目的:分析2011年和2014年我国西部少数民族边远地区农村居民现金卫生支出(out-of-pocket,ap)对贫困的影响。方法:利用2011年和2014年家庭入户调查数据,比较国家贫困标准下当地居民OOP发生前后的贫困发生率和贫困差距等指标。结果:2011年... 详细信息

目的:分析2011年和2014年我国西部少数民族边远地区农村居民现金卫生支出(out-of-pocket,ap)对贫困的影响。方法:利用2011年和2014年家庭入户调查数据,比较国家贫困标准下当地居民OOP发生前后的贫困发生率和贫困差距等指标。结果:2011年,OOP前后贫困发生率为31.07%和51.70%,致贫影响为20.63%,平均贫困差距增加284.24元,相对贫困差距增加183.73元;2014年,OOP前后贫困发生率为29.40%和44.66%,致贫影响为15.26%,平均贫困差距增加242.68元,相对贫困差距增加240.41元。结论:当地居民贫困发生率和平均贫困差距有所下降,但相对贫困差距上升;需要进一步调整新型农村合作医疗补偿方案,提高居民受益水平;重点关注低收入人群,减少因病致贫、因病返贫的现象。

关键词：居民现金卫生支出贫困发生率贫困差距致贫影响

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Discovery of a new family member of arsenic–vanadium polyoxometalates with high catalytic performance for one-pot coupling of CO2, epoxides and amines

引用

Tungsten 2025年第1期7卷 80-87页

作者： Fang-Qian Wang Yin Zhang Wei-Wei Wang Mei-Qian fu Hong-Bing Mo Wei-dong Yu Chao Liu Jun Yan College of Chemistry and Chemical Engineering Central South University Junior Education Department Changsha Normal University School of Science Hunan University of Technology and Business

Arsenic-vanadium polyoxometalates（POMs） represent a family of tremendous potential catalysts owing to their peculiar physical and chemical *** in CO2conversion,using POMs to catalyze the conversion of CO2and epoxide into chemical intermediates is the research ***,A new family number of arsenic-vanadium POMs,[N（CH3）4]4[As8V12O38（H2O）]·2H2O({As8V12O38}),was reported and characterized by single-crystal X-ray diffraction,fourier transform infrared spectroscopy,X-ray photoelectron spectroscopy,*** from other allotropicity,{As8V12O38}shows a different structure pattern with an I-43d *** obtained {As8V12O38} was employed as a catalyst for the *** initial catalytic reaction confirmed that {As8V12O38} can catalyze the formation of epoxy carbonate by using 1.01 kPa CO2and epoxide at 70℃.furtherly,when added amine in the catalytic system,oxazolidinone could be obtained by high yields,which provides an efficient way to convert CO2into profitable ***,a possible catalytic reaction mechanism was proposed by using different characterization analyses.{As8V12O38} first catalyzes the epoxide and CO2to form an epoxy carbonate and then catalyzes epoxy carbonate and benzylamine into oxazolidinone.

关键词： Arsenic CO2 conversion Oxazolidinone Polyoxometalates Vanadium

来源：

同方期刊数据库评论

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Human neural stem cell-derived extracellular vesicles protect against ischemic stroke by activating the PI3K/AKT/mTOR pathway

引用

Neural Regeneration Research 2025年第11期20卷 3245-3258页

作者： Jiayi Wang Mengke Zhao dong fu Meina Wang Chao Han Zhongyue Lv Liang Wang Jing Liu Stem Cell Clinical Research Center The First Affiliated Hospital of Dalian Medical UniversityDalianLiaoning ProvinceChina Dalian Innovation Institute of Stem Cell and Precision Medicine DalianLiaoning ProvinceChina Institute(College)of Integrative Medicine Dalian Medical UniversityDalianLiaoning ProvinceChina

Human neural stem cell-derived extracellular vesicles exhibit analogous functions to their parental cells,and can thus be used as substitutes for stem cells in stem cell therapy,thereby mitigating the risks of stem cell therapy and advancing the frontiers of stem cell-derived *** lays a foundation for the development of potentially potent new treatment modalities for ischemic ***,the precise mechanisms underlying the efficacy and safety of human neural stem cell-derived extracellular vesicles remain unclear,presenting challenges for clinical *** promote the translation of therapy based on human neural stem cell-derived extracellular vesicles from the bench to the bedside,we conducted a comprehensive preclinical study to evaluate the efficacy and safety of human neural stem cell-derived extracellular vesicles in the treatment of ischemic *** found that administration of human neural stem cell-derived extracellular vesicles to an ischemic stroke rat model reduced the volume of cerebral infarction and promoted functional recovery by alleviating neuronal *** human neural stem cell-derived extracellular vesicles reduced neuronal apoptosis by enhancing phosphorylation of phosphoinositide 3-kinase,mammalian target of rapamycin,and protein kinase B,and these effects were reversed by treatment with a phosphoinositide 3-kinase *** findings suggest that human neural stem cell-derived extracellular vesicles play a neuroprotective role in ischemic stroke through activation of phosphoinositide 3-kinase/protein kinase B/mammalian target of rapamycin signaling ***,we showed that human neural stem cell-derived extracellular vesicles have a good in vivo safety ***,human neural stem cell-derived extracellular vesicles are a promising potential agent for the treatment of ischemic stroke.

关键词： behavior exosome extracellular vesicles ischemic stroke mammalian target of rapamycin(mTOR) middle cerebral artery occlusion neural stem cells neuronal apoptosis phosphoinositide 3-kinase(PI3K) protein kinase B(AKT)

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Single-cell omics: experimental workflow, data analyses and applications

引用

Science China Life Sciences 2025年第1期68卷 5-102页

作者： Fengying Sun Haoyan Li dongqing Sun Shaliu fu Lei Gu Xin Shao Qinqin Wang Xin dong Bin Duan Feiyang Xing Jun Wu Minmin Xiao Fangqing Zhao Jing-dong J.Han Qi Liu Xiaohui Fan Chen Li Chenfei Wang Tieliu Shi Department of Clinical Laboratory the Affiliated Wuhu Hospital of East China Normal University (The Second People's Hospital of Wuhu City) Pharmaceutical Informatics Institute College of Pharmaceutical SciencesZhejiang University Key Laboratory of Spine and Spinal Cord Injury Repair and Regeneration (Tongji University) Ministry of EducationOrthopaedic DepartmentTongji HospitalBioinformatics DepartmentSchool of Life Sciences and TechnologyTongji University Frontier Science Center for Stem Cells School of Life Sciences and TechnologyTongji University Translational Medical Center for Stem Cell Therapy and Institute for Regenerative Medicine Shanghai East HospitalBioinformatics DepartmentSchool of Life Sciences and TechnologyTongji University Research Institute of Intelligent Computing Zhejiang Lab Shanghai Research Institute for Intelligent Autonomous Systems Center for Single-cell Omics School of Public HealthShanghai Jiao Tong University School of Medicine National Key Laboratory of Chinese Medicine Modernization Innovation Center of Yangtze River DeltaZhejiang University Center for Bioinformatics and Computational Biology Shanghai Key Laboratory of Regulatory Biologythe Institute of Biomedical Sciences and School of Life SciencesEast China Normal University Beijing Institutes of Life Science Chinese Academy of Sciences Peking-Tsinghua Center for Life Sciences Academy for Advanced Interdisciplinary StudiesCenter for Quantitative Biology (CQB)Peking University Zhejiang Key Laboratory of Precision Diagnosis and Therapy for Major Gynecological Diseases Women's HospitalZhejiang University School of Medicine Key Laboratory of Advanced Theory and Application in Statistics and Data Science-MOE School of StatisticsEast China Normal University

Cells are the fundamental units of biological systems and exhibit unique development trajectories and molecular features. Our exploration of how the genomes orchestrate the formation and maintenance of each cell, and control the cellular phenotypes of various organismsis, is both captivating and intricate. Since the inception of the first single-cell RNA technology, technologies related to single-cell sequencing have experienced rapid advancements in recent years. These technologies have expanded horizontally to include single-cell genome, epigenome,proteome, and metabolome, while vertically, they have progressed to integrate multiple omics data and incorporate additional information such as spatial sc RNA-seq and CRISPR screening. Single-cell omics represent a groundbreaking advancement in the biomedical field,offering profound insights into the understanding of complex diseases, including cancers. Here, we comprehensively summarize recent advances in single-cell omics technologies, with a specific focus on the methodology section. This overview aims to guide researchers in selecting appropriate methods for single-cell sequencing and related data analysis.

关键词： single-cell sequencing genome epigenome proteomics metabolomics multimodal spatial transcriptomics CRISPR screening

来源：

同方期刊数据库评论

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

The curative effect oligonucleotide on carcinoma of survivin antisense salivary adenoid cystic

引用

中国口腔颌面外科杂志 2008年第B5期6卷 150-150页

作者： Wei Cui fu sheng dong Jie Wang Xu Wang Hong Shi Department of Oral Pathology College of Stomatology Hebei Medical University. Shijiazhuang 050017 Department of Oral and Maxillofacial Surgery College of Stomatology Hebei Medical University. Shijiazhuang 050017 China

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

基于精益六西格玛的制药工艺设备故障预防研究

引用

今日自动化 2020年第4期 97-100页

作者：谢圣尧王富东苏州大学机电学院江苏苏州215123

目前某生物制药公司新建厂房刚投入运行,而工艺设备由于故障导致的停机较多,极有可能延误生产;提供研究工艺设备的故障情况并制定预防措施,保障生产正常开展,为生物制药公司工艺设备的维护提供参考。方法:该生物制药公司有一套工艺设备... 详细信息

目前某生物制药公司新建厂房刚投入运行,而工艺设备由于故障导致的停机较多,极有可能延误生产;提供研究工艺设备的故障情况并制定预防措施,保障生产正常开展,为生物制药公司工艺设备的维护提供参考。方法:该生物制药公司有一套工艺设备维护维修工单系统Maximo系统,在系统检索2018年全年工艺设备的维修工单信息,对每条工单进行数据分析。结果:2018年全年维修工单信息总计460条,非关键工艺设备故障75条,占比16.30%;间接影响设备故障177条,占比38.49%;关键影响设备202条,占比43.91%。最后经过一年的改善。2019年开始,参与改善的两类工艺设备月均故障数由0.71和0.38次每台每月,下降到0.41和0.06次每台每月,分别减少了42.25%和84.21%,P值分别为0.02和0.03,均小于0.05,显示出了良好的效果,达到了预期目的。结论:通过对历史故障工单数据的分析,不断了解工艺设备的状态和故障发生的原因,改善维护人员对设备的维护和加强维护人员的培训提供参考,保障生物制药企业正常生产。

关键词：工艺设备故障预防精益六西格玛

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：