检索结果-南通市图书馆

维普期刊数据库博看期刊

Effect of Exogenous MJA Treatment of Tea Plants on the Growth of Geometrid Larvae

在线全文

学校读者我要写书评

暂无评论

Agricultural Sciences in China 2005年第9期4卷 673-679页

作者： GUI Lian-you CHEN Zong-mao LIU shu-sheng Institute of Applied Entomology Zhejiang University Hangzhou 310029 P.R.China Key Laboratory of Tea Chemical Engineering Ministry of Agriculture/Tea Research Institute Chinese Academy of Agricultural Sciences Hangzhou 310008 P.R China Agricultural College Yangtze University Hubei 434025 P.R. China

The effect of tea plant Camellia sinensis induced by exogenous methyl jasmonate （MJA） on lipoxygenase （LOX）, polyphenol oxidase （PPO） and proteinase inhibitor （PI） activity in the leaves of tea plants, as well as the growth and midgut proteinase activity of the geometrid Ectropis obliqua larvae were studied. MJA significantly induced LOX, PPO and PI activity in leaves of tea plants. When geometrid larvae have fed on leaves of tea plants treated with MJA, the activities of the high alkaline trypsin-like enzyme and chymotrypsin-like enzyme in their midgut were significantly inhibited, but the activities of the low alkaline trypsin-like enzyme in their midgut were unaffected, leading to imbalance between different types of proteinase activity in the midgut of the larvae and in turn, the growth were inhibited. These chains of response may be an important mechanism of the direct resistance induced by MJA-treatment of tea plant on geometrid larvae.

关键词： Methyl jasmonate Proteinase inhibitors Midgut proteinase Ectropis obliqua Camellia sinensis

Boundedness for Multilinear Commutators of Bochner-Riesz Operator

在线全文

学校读者我要写书评

暂无评论

Journal of Mathematical Research and Exposition 2011年第6期31卷 1074-1080页

作者： Ru Long XIE Li sheng shu Qian Xiang ZHANG Department of Mathematics Chaohu UniversityAnhui 238000P.R.China Department of Mathematics Anhui Normal UniversityAnhui 241000P.R.China

In this paper,the boundedness for the multilinear commutators of Bochner-Riesz operator is *** prove that the multilinear commutators generated by Bochner-Riesz operator and Lipschitz function are bounded from Lp（Rn... 详细信息

关键词： multilinear commutators Bochner-Riesz operator Lipschitz function BMO space.

Functional CT for assessment of early vascular physiology in liver tumors

在线全文

学校读者我要写书评

暂无评论

Hepatobiliary & Pancreatic Diseases International 2008年第5期7卷 497-502页

作者： Jiang, Hui-Jie Zhang, Zai-Ren Shen, Bao-Zhong Wan, Yong Guo, Hong shu, sheng-Jie Harbin Med Coll Affiliated Hosp 4 Dept Med Imaging Harbin 150001 Peoples R China Harbin Med Coll Affiliated Hosp 2 CT Room Harbin 150086 Peoples R China

BACKGROUND: CT perfusion has been reported to have great advantages in detecting hepatic diseases. However, currently there are no studies showing the potential value of multi-slice CT perfusion with the deconvolution model method in diagnosing early hemodynamic changes caused by liver tumors. This study was undertaken to determine if early hemodynamic changes caused by liver tumors can be depicted with the perfusion imaging of multi-slice CT. METHODS: Ten New Zealand white rabbits before and after VX2 liver tumor inoculation served as the experimental animals. Ten normal rabbits served as controls. All underwent multi-slice CT perfusion for the measurement of hepatic blood flow (HBF), hepatic blood volume (HBV), mean transit time (MTT), permeability of capillary vessel surface (PS) and hepatic artery index (HAI). RESULTS: With the exception of MTT, which decreased significantly at the tumor periphery, HBF, HBV,. PS and HAI increased significantly compared with the surrounding normal tissue. All these changes occurred at days 5-9 after tumor inoculation. Statistically significant changes in these values were detected with tumor growth. CONCLUSIONS: The hemodynamic changes in the liver caused by rabbit VX2 liver tumor can be detected after tumor inoculation, and functional CT can evaluate the physiological characteristics of early tumor angiogenesis.

关键词： liver tumors computed tomography hemodynamics animal model

Viral infection of tobacco plants improves performance of Bemisia tabaci but more so for an invasive than for an indigenous biotype of the whitefly

在线全文

学校读者我要写书评

暂无评论

Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2010年第1期11卷 30-40页

作者： Jian LIU Meng LI Jun-min LI Chang-jun HUANG Xue-ping ZHOU Fang-cheng XU shu-sheng LIU Ministry of Agriculture Key Laboratory of Molecular Biology of Crop Pathogens and Insects Institute of Insect Sciences Zhejiang University Hangzhou 310029 China) Institute of Biotechnology Zhejiang University Hangzhou 310029 China Wenzhou Academy of Agricultural Sciences Wenzhou 325006 China

The ecological effects of plant-virus-vector interactions on invasion of alien plant viral vectors have been rarely investigated. We examined the transmission of Tomato yellow leaf cur/China virus （TYLCCNV） by the invasive Q biotype and the indigenous ZHJ2 biotype of the whitefly Bemisia tabaci, a plant viral vector, as well as the influence of TYLCCNV-infection of plants on the performance of the two whitefly biotypes. Both whitefly biotypes were able to acquire viruses from infected plants and retained them in their bodies, but were unable to transmit them to either tobacco or tomato plants. However, when the Q biotype fed on tobacco plants infected with TYLCCNV, its fecundity and longevity were increased by 7- and 1-fold, respectively, compared to those of the Q biotype fed on uninfected tobacco plants. When the ZHJ2 biotype fed on virus-infected plants, its fecundity and longevity were increased by only 2- and 0.5-fold, respectively. These data show that the Q biotype acquired higher beneficial effects from TYLCCNV-infection of tobacco plants than the ZHJ2 biotype. Thus, the Q biotype whitefly may have advantages in its invasion and displacement of the indigenous ZHJ2 biotype.

关键词： Bemisia tabaci Biotypes Begomovirus Vector-virus interaction Biological invasion

Effects of siRNA specific to the protein kinase CK2α on apoptosis of laryngeal carcinoma cells

在线全文

学校读者我要写书评

暂无评论

Chinese Medical Journal 2012年第9期125卷 1581-1585页

作者： WANG Jian-ting GONG shu-sheng Department of Otorhinolaryngology Head and Neck Surgery Beijing Chaoyang Hospital Capital Medical University Beijing 100020 China Department of Otorhinolaryngology Head and Neck Surgery Beijing Tongren Hospital Capital Medical University Beijing 100730 China

Background The relationship between apoptosis and tumors is a major focus in cancer research. RNA interference （RNAi） technology has emerged as a very potent tool to generate cellular knockdown phenotypes of a desired gene. The aim of this study was to explore the effect of siRNA specific to the protein casein kinase 2α （CK2α） on apoptosis of laryngeal carcinoma cells and to explore possible mechanisms.Methods An siRNA expression plasmid specific to CK2α, psiRNA-hH1neo-CK2α, and a non-specific siRNA expression plasmid, psiRNA-hH1neo-cont, were constructed and transfected into Hep-2 cells by a lipofectamine method. The mRNA and protein levels of CK2α in transfected cells were determined by reverse transcription-polymerase chain reaction （RT-PCR） and Western blotting analysis. Apoptosis was measured by Annexin V-fluorescein isothiocyanate （FITC）/ propidium iodide （PI） double-staining methods. The morphological changes to Hep-2 cells were observed under transmission electron microscopy （TEM）. The levels of Bcl-2 and Bax proteins were measured by Western blotting analysis.Results Levels of CK2α mRNA and protein were significantly decreased in the psiRNA-hH1neo-CK2α group compared to the other groups （P 〈0.05）. The apoptotic rate of the psiRNA-hH1neo-CK2α transfected group was significantly higher compared to that in the untransfected group and the siRNA-hH1neo-cont transfected group （25.66%±0.83%, 3.66%±0.43%, and 5.18%±0.22%）（P 〈0.05）. Compared with the untransfected group and the siRNA-hH1neo-cont transfected group, the psiRNA-hH1neo-CK2α transfected group presented with classical ultrastructural features of apoptosis, such as karyopyknosis, chromatic agglutination adjacent to the nuclear membrane, and apoptotic bodies. Compared with the other two groups, the level of Bcl-2 protein in the psiRNA-hH1neo-CK2α transfected group was decreased （0.20±0.09 vs. 0.72±0.16, 0.56±0.11, P 〈0.01）, while the Bax protein level was increased （0.81±0.17 vs. 0.26±0.12, 0.33±0.1

关键词： RNA interference small interfering RNA laryngeal neoplasms apoptosis casein kinase 2

Dual Reactive Oxygen Species Generator Independent of Light and Oxygen for Tumor Imaging and Catalytic Therapy

在线全文

学校读者我要写书评

暂无评论

CCS Chemistry 2022年第7期4卷 2321-2332页

作者： shu sheng Feng Liu Meng Meng Caina Xu Huayu Tian Xuesi Chen Key Laboratory of Polymer Ecomaterials Changchun Institute of Applied ChemistryChinese Academy of SciencesChangchun 130022 University of Chinese Academy of Sciences Beijing 100049 Jilin Biomedical Polymers Engineering Laboratory Changchun 130022 University of Science and Technology of China Hefei 230026

Currently,reactive oxygen species(ROS)generation primarily depends upon light and O2,which hampers its further biomedical ***,we report that amanganese(Ⅲ)salen-based complex(MnS)can continuously catalyze overexpressed hydrogen peroxide(H_(2)O_(2))in the tumor microenvironment to ^(1)O_(2),while the nanocarrier(MIL-100)as a Fenton reagent can convert H_(2)O_(2) to hydroxyl radicals(·OH)through the Fenton reaction,inducing noticeable intracellular DNA strand scission and lipid peroxidation to provoke tumor cell apoptosis without the involvement of light and O_(2).Moreover,MIL-100 depleted the antioxidant glutathione,further amplifying intracellular oxidative pressure,which in turn led to the self-degradation of MIL-100,suggesting the long-term biosafety of the *** to the excellent magnetic resonance imaging performance of MnS,the diagnosis and specific treatment of tumors were eventually *** work provides a novel approach for the realization of effective tumor catalytic therapy independent of light and O_(2) and a promising reference for the development of a wide range of catalytic therapeutic agents.

关键词： glutathione depletion reactive oxygen species biodegradable metal-organic framework magnetic resonance imaging catalytic therapy

The key cyclic electron flow protein PGR5 associates with cytochrome b_(6)f, and its function is partially influenced by the LHCⅡ state transition

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Horticulture Research 2021年第1期8卷 754-765页

作者： Xinyi Wu Jianqiang Wu Yu Wang Meiwen He Mingming He Weikang Liu sheng shu Jin Sun Shirong Guo College of Horticulture Nanjing Agricultural UniversityNanjing 210095China Nanjing Agricultural University(Suqian)Academy of Protected Horticulture JiangsuSuqian 223800China

In plants and algae,PGR5-dependent cyclic electron flow(CEF)is an important regulator of acclimation to fluctuating environments,but how PGR5 participates in CEF is *** this work,we analyzed two PGR5s in cucumber(Cucumis sativus L.)under different conditions and found that CsPGR5a played the dominant role in PGR5-dependent *** results of yeast two-hybrid,biomolecular fluorescence complementation(BiFC),blue native PAGE,and coimmunoprecipitation(CoIP)assays showed that PGR5a interacted with PetC,Lhcb3,and ***,the intensity of the interactions was dynamic during state transitions,and the abundance of PGR5 attached to cyt b_(6)f decreased during the transition from state 1 to state 2,which revealed that the function of PGR5a is related to the state *** proposed that PGR5 is a small mobile protein that functions when attached to protein complexes.

关键词： transition. state attached

Cloning of a putative extracellular Cu/Zn superoxide dismutase and functional differences of superoxide dismutases in invasive and indigenous whiteflies

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Insect Science 2015年第1期22卷 52-64页

作者： Xian-Long Gao Jun-Min Li Hong-Xing Xu Gen-Hong Yan Min Jiu shu-sheng Liu Xiao-Wei Wang Ministry of Agriculture Key Laboratory of Agricultural Entomology Institute of Insect Sciences Zhejiang University Hangzhou Instituteof Virology and Biotechnology Zhejiang Academy of Agricultural Sciences Hangzhou College of Food and Bioengineering HenanUniversity of Science and Technology Luoyang China

Superoxide dismutases （SODs） are a group of important antioxidant defense enzymes. In this study, a putative extracellular Cu/Zn superoxide dismutase （ecCuZnSOD） complementary DNA was cloned and characterized from the whitefly, Bemisia tabaci. Quantitative polymerase chain reaction analysis showed that the expression level of BtecCuZnSOD was more than 10-fold higher in the invasive Middle East Asia Minor 1 （MEAM1） than in the native Asia II 3 species of the B. tabaci species complex. After exposure to low temperature （4 ℃）, the expression of Bt-ecCuZnSOD gene was significantly up-regulated in MEAM1 but not in Asia II 3. Furthermore, the expression level ofB. tabaci intracellular CuZnSOD （Bt-icCuZnSOD）, Bt-ecCuZnSOD and mitochondrial MnSOD （Bt-mMnSOD） was compared after transferring MEAM1 and Asia II 3 whiteflies from favorable （cotton） to unfavorable host plants （tobacco）. On cotton, both CuZnSOD genes were expressed at a higher level in MEAM1 compared with Asia II 3. Interestingly, after transferring onto tobacco, the expression of Bt-ecCuZnSOD was significantly induced in Asia II 3 but not in MEAM1. On the other hand, while Bt-mMnSOD was expressed equally in both species on cotton, Bt-mMnSOD messenger RNA was up-regulated in MEAM 1 on tobacco. Consistently, enzymatic activity assays of CuZnSOD and MnSOD demonstrated that CuZnSOD might play an important protective role against oxidative stress in Asia II 3, whereas MnSOD activation was critical for MEAM1 whiteflies during host adaptation. Taken together, our results suggest that the successful invasion ofMEAM 1 is correlated with its constitutive high activity of CuZnSOD and inducible expression of MnSOD under stress conditions.

关键词： Bemisia tabaci extracellular Cu/Zn superoxide dismutase oxidative strees superoxide dismutase whitefly

Cochlear implant challenges encountered in tuberculous otitis media

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Asian Pacific Journal of Tropical Medicine 2012年第5期5卷 416-419页

作者： Cheng-Fang Chen Zhao-Hui Liu Jing Xie Xiao-Bo Ma Yi Li shu-sheng Gong Department of Otolaryngology Head and Neck SurgeryBeijing Tongren HospitalCapital Medical Unirersity Department of Radiology Beijing Tongren HospitalCapital Medical University Department of Otolaryngology Head and Neck SurgeryBeijing Tongren HospitalCapital Medical University

Tuberculous otitis media(TOM) is rare in ENT department,and is frequently misdiagnosed as otitis *** early systemic treatment is very important for *** reported a case report with TOM to highlight development of the disease and difficulties in clinical treatment in late stage of *** of ossified and eroded cochlea poses many unique challenges to both the surgeon and programming *** thorough preparation and complete knowledge about characters of specific issues,implantation would be performed successfully,and patients with ossified cochlear could benefit from cochlear implantation.

关键词： Tuberculous otitis media Cochlear implant Ossification Cochlear drill-out