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基于AHP方法的行星齿轮箱箱体阻尼敷设方案评价研究

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热能动力工程 2024年第3期39卷 200-206页

作者：魏博文常山栾圣罡张坤中国船舶集团有限公司第七〇三研究所黑龙江哈尔滨150078

针对行星齿轮箱箱体阻尼敷设方案减振效果评价问题,以某型行星变频调速齿轮箱为研究对象,基于层次分析法(AHP)建立阻尼敷设方案评价指标体系,以阻尼层厚度和阻尼材料损耗因子作为方案的定量指标,并结合方案的定性指标多方案对比分析,选... 详细信息

针对行星齿轮箱箱体阻尼敷设方案减振效果评价问题,以某型行星变频调速齿轮箱为研究对象,基于层次分析法(AHP)建立阻尼敷设方案评价指标体系,以阻尼层厚度和阻尼材料损耗因子作为方案的定量指标,并结合方案的定性指标多方案对比分析,选出最优的阻尼敷设方案。建立了5种箱体阻尼敷设方案的有限元模型,仿真分析得出模型机脚测点的振动加速度级。结果表明:阻尼层厚度和阻尼材料损耗因子对测点处机脚振动加速度级大小影响显著;选用SA-3为阻尼材料、阻尼厚度为14 mm的方案5,经指标体系评价确定为最优阻尼敷设方案,较原模型振动加速度级峰值平均下降了1.64 dB,优于其他4种阻尼敷设方案,验证了评价方法的正确性。

关键词：齿轮箱层次分析法综合评价阻尼减振

A SPX domain vacuolar transporter links phosphate sensing to homeostasis in Arabidopsis

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Molecular Plant 2022年第10期15卷 1590-1601页

作者： Mingda luan Fugeng Zhao Guangfang Sun Min Xu Aigen Fu Wenzhi Lan sheng luan Key Laboratory of Resource Biology and Biotechnology in Western China Ministry of EducationProvincial Key Laboratory of BiotechnologyCollege of Life SciencesNorthwest UniversityXi'an 710069China Institute of Future Agriculture Northwest Agriculture and Forestry UniversityYanglingShaanxi 712100China School of Life Sciences Nanjing UniversityNanjingJiangsu 210023China Department of Plant and Microbial Biology University of CaliforniaBerkeleyBerkeleyCA 94720USA

Excess phosphate(Pi)is stored into the vacuole through Pi transporters so that cytoplasmic Pi levels remain stable in plant *** hypothesized that the vacuolar Pi transporters may harbor a Pi-sensing mechanism so that they are activated to deliver Pi into the vacuole only when cytosolic Pi reaches a threshold high *** tested this hypothesis using Vacuolar Phosphate Transporter 1(VPT1),a SPX domain-containing vacuolar Pi transporter,as a *** studies have defined SPX as a Pi-sensing module that binds inositol polyphosphate signaling molecules(InsPs)produced at high cellular Pi *** showed here that Pi-deficient conditions or mutation of the SPX domain severely impaired the transport activity of *** further identified an auto-inhibitory domain in VPT1 that suppresses its transport *** together the results from detailed structure-function analyses,our study suggests that VPT1 is in the auto-inhibitory state when Pi status is low,whereas at high cellular Pi status InsPs are produced and bind SPX domain to switch on VPT1 activity to deliver Pi into the *** thus provides an auto-regulatory mechanism for VPT1-mediated Pi sensing and homeostasis in plant cells.

关键词： VPT1 Pi signaling autoinhibition transport activity inositol phosphates

From Receptor-Like Kinases to Calcium Spikes： What Are the Missing Links？

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Molecular Plant 2014年第10期7卷 1501-1504页

作者： Feng Yu[a],, Wang Tian[b], sheng luan[b],[c], a Hunan Province Key Laboratory of Plant Functional Genomics and Developmental Regulation Hunan University Changsha 410082 P.R. Chinab Department of Plant and Microbial Biology University of California Berkeley CA 94720 USAc NJU-NJFU Joint Institute for Plant Molecular Biology School of Life Sciences Nanjing University Nanjing 210093 P.R. China

As sessile organisms, plants have evolved with complex signaling networks to cope with the constantly changing environment. Receptor-like kinases （RLKs） and calcium both play important roles during the early stage of envi- ronmental responses in plants. RLKs are trans-membrane receptor kinases that sense extracellular signals, such as hormones, peptides, and pathogens, and trigger sig- nal transduction pathways leading to cellular responses （Osakabe et al., 2013）.

关键词： ARABIDOPSIS PERCEPTION ATPEPR1

零鱼粉饲料与高鱼粉饲料饲喂下凡纳对虾饲料利用效率相关性状的遗传评估

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渔业科学进展 2024年第5期45卷 174-182页

作者：朱相燕孟宪红代平孔杰栾生曹家旺谭建邢群浙江海洋大学水产学院浙江舟山316022 中国水产科学研究院黄海水产研究所、海水养殖生物育种与可持续产出全国重点实验室山东青岛266071 青岛海洋科技中心海洋渔业科学与食物产出过程功能实验室山东青岛266237 邦普种业科技有限公司山东潍坊261311

为探讨选育零鱼粉需求凡纳对虾(Penaeus vannamei)品种的可能性,解决目前鱼粉短缺、饲料成本居高不下的问题,本研究在零鱼粉饲料(蛋白含量占38%)和高鱼粉饲料(鱼粉含量占25%,蛋白含量占42%)饲喂条件下进行凡纳对虾饲料利用效率相关性状... 详细信息

为探讨选育零鱼粉需求凡纳对虾(Penaeus vannamei)品种的可能性,解决目前鱼粉短缺、饲料成本居高不下的问题,本研究在零鱼粉饲料(蛋白含量占38%)和高鱼粉饲料(鱼粉含量占25%,蛋白含量占42%)饲喂条件下进行凡纳对虾饲料利用效率相关性状的遗传评估。选择30个家系进行40 d的单尾饲养实验,结果显示,饲喂高鱼粉饲料对虾的平均增重率(62.00%)和平均饲料效率比(124.00%)明显高于饲喂零鱼粉饲料(23.50%和49.40%)。对虾摄食量和增重率在零鱼粉饲料和高鱼粉饲料饲喂下的遗传力在0.458±0.140到0.699±0.155之间,饲料效率比在零鱼粉饲料和高鱼粉饲料饲喂下的遗传力分别为0.186±0.098和0.341±0.110。由于没有剖分出共同环境效应,遗传力都存在一定程度高估,因此,零鱼粉饲料饲喂下的饲料效率比的遗传变异并不高。摄食量和增重率在2种饲料间的遗传相关分别为0.580±0.171和0.676±0.155,表示这2个性状存在中等的基因型与饲料互作效应。饲料效率比在2种饲料间的遗传相关仅为0.299±0.304,表示该性状存在严重的基因型与饲料互作效应。综上结果来看,使用零鱼粉饲料不利于凡纳对虾的养殖及选育工作的开展。

关键词：凡纳对虾饲料效率比遗传力遗传相关基因型与饲料互作

凡纳滨对虾在低氧环境下存活性状的遗传参数评估

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渔业科学进展 2024年第1期45卷 138-147页

作者：段毓佳谭建栾生罗坤王宏杰隋娟孟宪红孔杰湖州师范学院浙江省水生生物资源养护与开发技术研究重点实验室、中国水产科学研究院水生动物繁育与营养重点实验室浙江湖州313000 中国水产科学研究院黄海水产研究所农业农村部海洋渔业与可持续发展重点实验室山东青岛266071 海洋渔业科学与食物产出过程功能实验室山东青岛266071 邦普种业科技有限公司山东潍坊261000

在水产养殖中,尤其是高密度养殖情况下,缺氧会短期内造成个体的大量死亡,从而影响生产效益。本研究以凡纳滨对虾(Litopenaeus vannamei)高抗和快大2个品系作为研究对象,利用高致死溶解氧(DO)胁迫的方式,对2个品系各41个家系共计6560尾... 详细信息

在水产养殖中,尤其是高密度养殖情况下,缺氧会短期内造成个体的大量死亡,从而影响生产效益。本研究以凡纳滨对虾(Litopenaeus vannamei)高抗和快大2个品系作为研究对象,利用高致死溶解氧(DO)胁迫的方式,对2个品系各41个家系共计6560尾幼虾进行低氧耐受性测试,统计高致死溶解氧水平下幼虾的存活性状,分析不同品系间及同一品系不同家系间耐低氧性状的差异,评估其遗传参数;对耐受性有显著差异的家系个体的鳃、肌肉、肝胰腺组织进行组织学观察,并比较其细胞水平上的差异。结果显示,在0.3mg/L低氧条件下,高抗品系和快大品系的家系半致死存活率SS50均表现出显著差异(P<0.05),高抗品系SS50为49.30%,快大品系SS50为42.52%;家系间存活时间的变异系数分别为60%和45%。利用阈值性状动物模型,以半致死时的个体存活状态作为观测值(存活为1,死亡为0)估算的遗传参数为(0.345±0.031)~(0.378±0.029),经过转换后在连续变化的观测值尺度上的遗传力为(0.219±0.031)~(0.237±0.029),表现为中等遗传力水平。高抗品系中低氧耐受家系和低氧敏感家系的鳃、肌肉和肝胰腺组织均出现不同程度的损伤,但不同的家系间表现出不同程度的抗逆生理特征差异,这可能与高抗不同家系抗逆耐受力强弱有关。不同家系间在低氧胁迫下的存活时间、生理特性差异以及各品系遗传力估算结果表明,凡纳滨对虾对高致死溶解氧水平的耐受性状存在丰富的遗传变异,具有选育的可行性。

关键词：凡纳滨对虾低溶解氧遗传力组织损伤选育

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Estimating genetic parameters with molecular relatedness and pedigree reconstruction for growth traits in early mixed breeding of juvenile turbot

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Acta Oceanologica Sinica 2021年第9期40卷 66-73页

作者： Song Sun Weiji Wang Yulong Hu sheng luan Ding Lyu Jie Kong College of Fisheries and Life Science Shanghai Ocean UniversityShanghai 201306China Laboratory for Marine Fisheries Science and Food Production Processes Pilot National Laboratory for Marine Science and Technology(Qingdao)Qingdao 266071China Key Laboratory for Sustainable Development of Marine Fisheries Resources of Ministry of Agriculture Yellow Sea Fisheries Research InstituteChinese Academy of Fishery SciencesQingdao 266071China

An introduced turbot population was used to establish families and to estimate genetic parameters of the offspring. However, there is a lack of pedigree information, and common environmental effects can be introduced when each full-sib family is raised in a single tank. Therefore, in the genetic evaluation, SSRs(simple sequence repeats) were used to reconstruct the pedigree and to calculate molecular relatedness between individuals, and the early mixed-family culture model was used to remove the impact of the common environmental effects. After100 d of early mixed culture, twenty SSRs were used to cluster 20 families and to calculate paired molecular relationships(n=880). Additive genetic matrices were constructed using molecular relatedness(MR) and pedigree reconstruction(PR) and were then applied to the same animal model to estimate genetic parameters. Based on PR, the heritabilities for body weight and body length were 0.214±0.124 and 0.117±0.141, and based on MR they were 0.101±0.031 and 0.102±0.034, respectively. Cross validation showed that the accuracies of the estimated breeding values based on MR(body weight and body length of 0.717±0.045 and 0.629±0.141, respectively) were higher than those of PR(body weight and body length of 0.692±0.052 and 0.615±0.060, respectively). The MR method ensure availability of all genotyped selection candidates, thereby improving the accuracy of the breeding value estimation.

关键词： turbot SSR genetic parameter mixed breeding

Genetic Parameters of Body Length and Response to Selection for Growth Across Four Generations of Artemia sinica

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Journal of Ocean University of China 2019年第1期18卷 203-209页

作者： KONG Zhangwei KONG Jie luan sheng ZHANG Zhiwei YU Chifang LUO Kun College of Marine Life Sciences Ocean University of China Key Laboratory for Sustainable Utilization of Marine Fisheries Resources Ministry of Agriculture Yellow Sea Fisheries Research Institute Chinese Academy of Fishery Sciences Laboratory for Marine Fisheries Sciences and Food Production Processes Qingdao National Laboratory for Marine Science and Technology

To investigate the genetic components of growth in the brine shrimp Artemia sinica, we estimated the genetic parameters of body length and the response to selection using a fully pedigreed population of A. sinica. The base population was generated from four wild founder populations. We tested 4160 offspring in 360 families over four generations for growth and survival performance. Across four generations, we produced full-and half-sib families with nested mating, where two dams were mated to the same sire. Individual body length was measured for each nauplius at day 20 post-hatching. Heritability of body length was estimated across four generations with the restricted maximum likelihood method. The heritability of body length in A. sinica was low(0.14 ± 0.05), and the common environmental effect was 0.14 ± 0.02. We estimated the response to selection for body length by calculating the difference in the mean breeding values between different generations. The accumulated genetic gain in body length was 278.94 μm after three generations of selection. This low response to selection was probably caused by the low heritability of body length, small sample size, and the low selection intensity(50%). The results suggest that A. sinica selective breeding programs must be changed to generate any substantial, sustainable genetic increases in body length. We suggest that optimal genetic gains could be achieved by introducing wild strains into the nuclear breeding population to increase genetic variation, and by increasing the size of the breeding population to allow for increased selection intensity.

关键词： Artemia sinica body length heritability selection responses

A DTX/MATE-Type Transporter Facilitates Abscisic Acid Efflux and Modulates ABA Sensitivity and Drought Tolerance in Arabidopsis

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Molecular Plant 2014年第10期7卷 1522-1532页

作者： Haiwen Zhang Huifen Zhu Yajun Pan Yuexuan Yu sheng luan Legong Li College of Life Sciences Capital Normal University Beijing 100048 China Department of Plant and Microbial Biology University of California Berkeley CA 94720 USA Institute of Plant Molecular Biology School of Life Sciences Nanjing University Nanjing 210093 China

Abscisic acid （ABA） regulates numerous physiological and developmental processes in plants. Recent studies identify intracellular ABA receptors, implicating the transport of ABA across cell membranes as crucial for ABA sensing and response. Here, we report that a DTX/Multidrug and Toxic Compound Extrusion （MATE） family member in Arabidopsis thaliana, AtDTX50, functions as an ABA efflux transporter. When expressed heterologously in both an Escherichia coli strain and Xenopus oocyte cells, AtDTX50 was found to facilitate ABA efflux. Furthermore, dtx50 mutant mesophyll cells preloaded with ABA released less ABA compared with the wild-type （WT）. The AtDTX50 gene was expressed mainly in the vascular tissues and guard ceils and its expression was strongly up-regulated by exogenous ABA. The AtDTX50：：GFP fusion protein was localized predominantly to the plasma membrane. The dtx50 mutant plants were observed to be more sensitive to ABA in growth inhibition. In addition, compared with the WT, dtx50 mutant plants were more tolerant to drought with lower stomatal conductance, consistent with its function as an ABA efflux carrier in guard cells.

关键词： hormone biology molecular transport Arabidopsis.

ABA Signaling in Guard Cells Entails a Dynamic Protein-Protein Interaction Relay from the PYL-RCAR Family Receptors to Ion Channels

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Molecular Plant 2013年第2期6卷 528-538页

作者： Sung Chul Lee Chae Woo Lim Wenzhi Lan Kai He sheng luan Department of Plant and Microbial Biology University of California Berkeley CA 94720 USA School of Biological Sciences (BK21 Program) Chung-Ang University Seoul 156-756 Korea Plant Molecular Biology Institute and College of Life Sciences Nanjing University China WCU Program Chonnam National University Gwangju Korea

Plant hormone abscisic acid （ABA） serves as an integrator of environmental stresses such as drought to trig-ger stomatal closure by regulating specific ion channels in guard cells. We previously reported that SLAC1, an outward anion channel required for stomatal closure, was regulated via reversible protein phosphorylation events involving ABA signaling components, including protein phosphatase 2C members and a SnRK2-type kinase （OST1）. In this study, we reconstituted the ABA signaling pathway as a protein-protein interaction relay from the PYL/RCAR-type receptors, to the PP2C-SnRK2 phosphatase-kinase pairs, to the ion channel SLAC1. The ABA receptors interacted with and inhibited PP2C phosphatase activity against the SnRK2-type kinase, releasing active SnRK2 kinase to phosphorylate, and activate the SLAC1 channel, leading to reduced guard cell turgor and stomatal closure. Both yeast two-hybrid and bimolecular fluorescence complementation assays were used to verify the interactions among the components in the pathway. These biochemical assays demonstrated activity modifications of phosphatases and kinases by their interaction partners. The SLAC1 channel activity was used as an endpoint readout for the strength of the signaling pathway, depending on the presence of different combinations of signaling components. Further study using transgenic plants overexpressing one of the ABA receptors demonstrated that changing the relative level of interacting partners would change ABA sensitivity.

关键词： abscisic acid ABA receptor protein kinase protein phosphatase SLAC1.