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Universal peroxidase-like strategy for sensitive glucose detection in complex matrix

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Nano Research 2023年第1期16卷 1141-1148页

作者： Guohua Jiang Zhaoyan Yang Kai Zhu shenfei zong Lei Wu Zhuyuan Wang Yiping Cui Advanced Photonics Center Southeast UniversityNanjing 210096China

Glucose detection in complex matrix such as physiological fluids and drinks can provide useful information guide for people.However,traditional detection methods toward complex matrix suffer from the impurity interference or complex pretreatments.So,it is important to exploit a universal and sensitive glucose detection strategy in complex matrix.In this work,a cascade catalytic scheme based on peroxidase-like MBs@MIL-100(Fe)@Ag(MMA)is developed for sensitive glucose detection in complex solution.Using 3,3’,5,5’-tetramethylbenzidine(TMB)as an indicator,MMA can trigger catalytic cascade reactions for specific glucose sensing.In particular,the peroxidase-like MIL-100(Fe)serves as both the catalysis unit and enrichment unit.Oxidation state of TMB(oxTMB)can be effectively and specifically enriched by MIL-100(Fe)to exclude the interference of undesired impurities and macromolecules,which is suitable for complex sample matrix including colored soda and saliva.In addition,utilizing the peroxidase-like activity of MIL-100(Fe)for self-clean,the residual indicator molecules can be degraded,resulting in the recyclable use of MMA.

关键词： surface enhanced Raman scattering complex matrix MIL-100(Fe) glucose detection recyclable

In situ probing of cell-cell communications with surface- enhanced Raman scattering （SERS） nanoprobes and microfluidic networks for screening of immunotherapeutic drugs

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Nano Research 2017年第2期10卷 584-594页

作者： Lei Wu Zhuyuan Wang Yizhi Zhang Jiayuan Fei Hui Chen shenfei zong Yiping Cui Advanced Photonics Center School of Electronic Science and Engineering Southeast University Sipailou 2# Nanjing 210096 China

Discovering novel drugs for cancer immunotherapy requires a robust in vitro drug screening platform that allows for straightforward probing of cell-ceil communications. Here, we combined surface-enhanced Raman scattering （SERS） nanoprobes with microfluidic networks to monitor in situ the cancer-immune system intercellular communications. The microfluidic platform links up immune cells with cancer cells, where the cancer-cell secretions act as signaling mediators. First, gold@silver core--shell nanorods were employed to fabricate SERS immunoprobes for analysis of the signaling molecules. Multiple cancer secretions in a tumor microenvironment were quantitatively analyzed by a SERS-assisted three-dimensional （3D） barcode immunoassay with high sensitivity （1 ng/mL）. Second, in an on-chip cell proliferation assay, multiple immunosuppressive proteins secreted by cancer cells were found to inhibit activation of immune cells, indicating that the platform simulates the physiological process of cancer-immune system communications. Furthermore, potential drug candidates were tested on this platform. A quantitative SERS immunoassay was performed to evaluate drug efficacy at regulating the secretion behavior of cancer cells and the activity of immune cells. This assay showed the suitability of this platform for in vitro drug screening. It is expected that the fully integrated and highly automated SERS-microfluidic platform will become a powerful analytical tool for probing intercellular communications and should accelerate the discovery and clinical validation of novel druKs.

关键词： cell-cell communication,microfluidic chip,surface-enhanced Raman scattering （SERS）,barcode immunoassay,drug discovery

Growth performance, serum parameters, inflammatory responses,intestinal morphology and microbiota of weaned piglets fed 18% crude protein diets with different ratios of standardized ileal digestible isoleucine to lysine

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Animal Nutrition 2024年第1期16卷 313-325页

作者： Jian Wang Sujie Liu Jiayu Ma Xiaoli Dong shenfei Long Xiangshu Piao State Key Laboratory of Animal Nutrition College of Animal Science and Technology China Agricultural University CJ International Trading Co. Ltd Beijing Jingwa Agricultural Science and Technology Innovation Center

The present study was to explore the Ile requirement of piglets fed 18% crude protein(CP) diets. Two hundred and fifty 28-day-old Duroc × Landrace × Yorkshire piglets(8.37 ± 1.92 kg) were randomly divided into 5 dietary treatments(10 piglets per replicate, 5 barrows and 5 gilts per replicate) with 45%,50%, 55%, 60%, 65% standardized ileal digestible(SID) Ile-to-Lys ratios, and the SID Lys was formulated to1.19%. The experimental design consisted of two phases(d 1 to 14 and d 15 to 28). Results showed that average daily gain(ADG) had a tendency to quadratically increase as the SID Ile-to-Lys ratio increased(P = 0.09), and the optimum SID Ile-to-Lys ratios required to maximize ADG were 48.33% and 54.63% for broken-line linear model and quadratic polynomial model, respectively. Different SID Ile-to-Lys ratios had no significant effects on average daily feed intake and gain-to-feed ratio. Dry matter(P < 0.01), CP(P = 0.01), ether extract(P = 0.04), gross energy(P < 0.01) and organic matter(P < 0.01) digestibility increased quadratically. Serum total cholesterol levels decreased linearly(P = 0.01) and quadratically(P < 0.01); aspartate aminotransferase(P < 0.01), interleukin-1β(P = 0.01), and tumor necrosis factor-a(P < 0.01) levels decreased quadratically; immunoglobulin G(P = 0.03) and immunoglobulin M(P = 0.01)concentrations increased quadratically. Serum Ser levels decreased linearly(P < 0.01) and quadratically(P = 0.01); Glu(P = 0.02), Arg(P = 0.05), and Thr(P = 0.03) levels decreased quadratically; Gly(P < 0.01)and Leu(P = 0.01) levels decreased linearly; Ile(P < 0.01) concentration increased linearly. Duodenal villus height(P < 0.01) and villus height to crypt depth ratio(P < 0.01) increased quadratically. The deficiency or excess of Ile decreased short chain fatty acid-producing bacteria abundance and increased pathogenic bacteria abundance. Overall, taking ADG as the effect index, the optimum SID Ile-to-Lys ratios of piglets offered 18% CP diets were 48.33% and 54.63% based on two different statistical models

关键词： Weaned piglet Growth performance Intestinal health Isoleucine Reduced crude protein diet

Biomass-derived porous carbon with single-atomic cobalt toward high-performance aqueous zinc-sulfur batteries at room temperature

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Journal of Energy Chemistry 2024年第8期95卷 325-335页

作者： shenfei Zhao Xiaoshuai Wu Jiliang Zhang Chunjie Li Zixiang Cui Weihua Hu Ruguang Ma Changming Li Institute for Clean Energy & Advanced Materials School of Materials & Energy Southwest University Institute for Materials Science and Devices School of Materials Science and Engineering Suzhou University of Science and Technology School of Materials Science and Engineering Dalian Jiaotong University

Aqueous zinc-sulfur batteries at room temperature hold great potential for next-generation energy storage technology due to their low cost, safety and high energy density. However, slow reaction kinetics and high activation energy at the sulfur cathode pose great challenges for the practical applications. Herein,biomass-derived carbon with single-atomic cobalt sites（MMPC-Co） is synthesized as the cathode in Zn-S batteries. The catalysis of single-atom Co sites greatly promotes the transform of cathode electrolyte interface（CEI） on the cathode surface, while offering accelerated charge transfer rate for high conversion reversibility and large electrochemical surface area（ECSA） for high electrocatalytic current. Furthermore,the rich pore structure not only physically limits sulfur loss, but also accelerates the transport of zinc ions.In addition, the large pore volume of MMPC-Co is able to relieve the stress effect caused by the volume expansion of Zn S during charge/discharge cycles, thereby maintaining the stability of electrode structure.Consequently, the sulfur cathode maintains a high specific capacity of 729.96 m A h g-1after 500 cycles at4 A g-1, which is much better than most cathode materials reported in the literature. This work provides new insights into the design and development of room-temperature aqueous Zn-S batteries.

关键词： Aqueous zinc-sulfur batteries Biomass-derived carbon Rich pore structure Single-atom catalysts Sulfur cathode

基于功能化水凝胶的肿瘤源性外泌体高灵敏检测

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光学学报 2023年第21期43卷 237-245页

作者：杨朝雁赵书瑾王子烨刘娇宗慎飞王元李炳祥崔一平南京邮电大学电子与光学工程学院柔性电子(未来技术)学院江苏南京210023 东南大学电子科学与工程学院江苏南京210096

提出一种外泌体检测新方法,通过将表面增强拉曼散射(SERS)纳米探针固定在核酸适体(DNA)功能化水凝胶中,实现对肿瘤源性外泌体的高灵敏度光学检测。SERS纳米探针被用于识别肿瘤源性外泌体并产生指纹光学信号。SERS活性DNA功能化水凝胶(... 详细信息

提出一种外泌体检测新方法,通过将表面增强拉曼散射(SERS)纳米探针固定在核酸适体(DNA)功能化水凝胶中,实现对肿瘤源性外泌体的高灵敏度光学检测。SERS纳米探针被用于识别肿瘤源性外泌体并产生指纹光学信号。SERS活性DNA功能化水凝胶(简称“SD水凝胶”)作为传感器,不仅提供了用于生物识别的三维反应位点,而且可放大SERS纳米探针的光学信号。选择性地与靶外泌体结合后,SERS纳米探针脱离SD水凝胶,导致SERS信号减弱,从而实现光学检测。通过SERS信号变化,SD水凝胶可以定量、灵敏地检测肿瘤源性外泌体,浓度检测限(LOD)约为22μL^(-1)。该SD水凝胶将为临床癌症诊断提供一种新的技术手段。

关键词：生物光学表面增强拉曼散射光谱技术光学检测外泌体纳米探针水凝胶

Determination of the available energy,standardized ileal digestibility of amino acids of fermented corn germ meal replacing soybean meal in growing pig diets

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Animal Nutrition 2022年第2期9卷 259-268页

作者： Tengfei He Yuhui Zheng Xiangshu Piao shenfei Long State Key Laboratory of Animal Nutrition College of Animal Science and TechnologyChina Agricultural UniversityBeijing 100193China

Three experiments were conducted to compare the digestible(DE),metabolizable energy(ME),and standardized ileal digestibility(SID)of amino acids(AA)in fermented corn germ meal(FCGM)and soybean meal(SBM),and evaluate the effects of FCGM replacing SBM in growing pig diets.In Exp.1,18 barrows with initial body weight(BW)of 60.2±3.40 kg were randomly allotted to 3 treatments with 6 replicates per treatment.The control diet used corn as the only energy ingredient,and 2 test diets were made by replacing 25.8%of corn with FCGM or SBM.The DE and ME of FCGM were less(P<0.01)than those of SBM.In Exp.2,18 barrows(59.3±2.52 kg BW)with ileal T-cannulas were randomly allotted to 3 treatments with 6 replicates per treatment.The SID of arginine,tryptophan and proline were greater(P<0.05)and the tyrosine was less(P=0.01)in SBM compared with FCGM.In Exp.3,144 growing pigs(56.1±5.22 kg BW)were randomly allotted to 4 treatments with 6 pens per treatment(3 barrows and 3 gilts per pen).Four diets(FCGM0,FCGM10,FCGM20 and FCGM30)were formulated using FCGM to replace 0%,10%,20%and 30%of SBM,respectively.The ME and SID values of AA of SBM and FCGM were determined by Exp.1 and 2.Results showed that increasing FCGM inclusion quadratically(P<0.05)increased the average daily gain(ADG),average daily feed intake,and the levels of serum immunoglobulin G(IgG)and urea nitrogen,and linearly(P<0.05)increased the serum IgM,the propanoic acid,butyric acid,total volatile fatty acid(VFA)and the Shannon index of microbiota in feces.Besides,the relative abundance of genus Streptococcus in FCGM0,Lactobacillus in FCGM10 and Lachnospiraceae in FCGM30 were increased(P<0.05)compared with other treatments.In conclusion,we recommend replacing 11.80%of SBM with FCGM to obtain the optimal ADG of growing pigs.Moreover,as the ratio of FCGM replacing SBM increased in diet,the immunity,intestinal microbiota and total VFA composition of growing pigs were improved.

关键词： Available energy Amino acid digestibility Fermented corn germ meal Soybean meal Growing pig

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多脉冲加载下PBX装药的应力放大效应

爆炸与冲击 2024年第6期44卷 26-38页

作者：何杨胡秋实仲苏洋廖深飞李涛傅华中国工程物理研究院流体物理研究所四川绵阳621999

针对弹体侵彻过程中装药常常受到多脉冲载荷作用的问题,提出了一种装药多脉冲加载装置,研究了多脉冲加载下装药的应力放大效应。基于集中质量法建立了多脉冲加载装置的等效弹簧模型,对产生应力放大的条件进行了探讨。结果表明,多脉冲载... 详细信息

针对弹体侵彻过程中装药常常受到多脉冲载荷作用的问题,提出了一种装药多脉冲加载装置,研究了多脉冲加载下装药的应力放大效应。基于集中质量法建立了多脉冲加载装置的等效弹簧模型,对产生应力放大的条件进行了探讨。结果表明,多脉冲载荷频率与装药固有频率匹配时系统发生共振,装药产生响应放大,放大倍数随结构间隙宽度的增加而降低。装药多脉冲加载下存在一个时间区间,撞击加载的发生时刻落在该区间内时系统可产生放大效果。对高聚物黏结炸药(polymer bonded explosive,PBX)模拟材料,实现了实验室条件下应力幅值百兆帕、脉冲间隔毫秒级、脉冲次数3次且幅值逐渐放大的多脉冲载荷加载。

关键词：侵彻多脉冲应力放大集中质量法 PBX炸药

The interaction among gut microbes,the intestinal barrier and short chain fatty acids

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Animal Nutrition 2022年第2期9卷 159-174页

作者： Jiayu Ma Xiangshu Piao Shad Mahfuz shenfei Long Jian Wang State Key Laboratory of Animal Nutrition College of Animal Science and TechnologyChina Agricultural UniversityBeijing 100193China Department of Animal Nutrition Sylhet Agricultural UniversitySylhet 3100Bangladesh

The mammalian gut is inhabited by a massive and complicated microbial community, in which the hostachieves a stable symbiotic environment through the interdependence, coordination, reciprocal constraintsand participation in an immune response. The interaction between the host gut and themicrobiota is essential for maintaining and achieving the homeostasis of the organism. Consequently, guthomeostasis is pivotal in safeguarding the growth and development and potential productive performanceof the host. As metabolites of microorganisms, short chain fatty acids are not only the preferredenergy metabolic feedstock for host intestinal epithelial cells, but also exert vital effects on antioxidantsand the regulation of intestinal community homeostasis. Herein, we summarize the effects of intestinalmicroorganisms on the host gut and the mechanisms of action of short chain fatty acids on the fourintestinal barriers of the organism, which will shed light on the manipulation of the intestinal communityto achieve precise nutrition for specific individuals and provide a novel perspective for theprevention and treatment of diseases.

关键词： Short chain fatty acids Gut microbiota Intestinal barrier Intestinal epithelium cells Interaction mechanism

Microencapsulated essential oils combined with organic acids improves immune antioxidant capacity and intestinal barrier function as well as modulates the hindgut microbial community in piglets

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Journal of Animal Science and Biotechnology 2022年第4期13卷 1126-1142页

作者： Jiayu Ma shenfei Long Jian Wang Jie Gao Xiangshu Piao State Key Laboratory of Animal Nutrition College of Animal Science and TechnologyChina Agricultural UniversityBeijing 100193China Institute of Animal Sciences Chinese Academy of Agricultural SciencesBeijing 100193China.

Background:The objective of this experiment was to evaluate the effect of a combination of microencapsulated essential oils and organic acids(MOA)on growth performance,immuno-antioxidant status,intestinal barrier function and microbial structure of the hindgut in piglets.A total of 120 piglets(Duroc×[Landrace×Yorkshire];weighted 7.66±1.79 kg,weaned at d 28)were randomly selected and allocated to 3 treatments with 4 replicates per group and 10 piglets per replicate according to the initial body weight and gender.The dietary treatments were as follows:1)basal diet(Ctrl);2)Ctrl+chlortetracycline(75 mg/kg)(AGP);3)Ctrl+MOA(1500 mg/kg).The experiment period was lasted for 21 d.Results:Compared to the Ctrl group,dietary supplemented MOA alleviated(P<0.05)the diarrhea rate from d 12 to 21,enhanced(P<0.05)the concentration of serum interlukin-10 and glutathione peroxidase in piglets on d 11 after weaning and serum superoxide dismutase in 21-day piglets.The MOA group also improved(P<0.05)the apparent digestibility of dry matter(DM),organic matter(OM)and gross energy(GE),up-regulated(P<0.05)the mRNA expression level of occludin,claudin-1 and mucin-2 in ileum and increased(P<0.05)the contents of propionic and butyric acids in the cecum of piglets.The MOA group modulated the cecal and colonic microbial community structure and increased(P<0.05)the abundance of Faecalibacterium and Muribaculaceae in cecum and Streptococcus and Weissella in colon.Additionally,AGP group decreased(P<0.05)apparent digestibility of DM,OM and GE as well as down-regulated(P<0.05)relative gene expression level of claudin-1 in duodenum and jejunum,ZO-1 and mucin-1 in jejunum of piglets.Conclusion:In summary,dietary supplemented MOA alleviated diarrhea and improved nutrient apparent digestibility in piglets via enhancing immuno-antioxidant properties,increasing digestive enzyme activity,upregulating the expression of intestinal barrier-related genes,and modifying the microbial community structure of the cecum and colon.Therefore,dietary supplementation w

关键词： Essential oil Gut microbiota Intestinal barrier Mixed organic acid Piglets