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Regulatory effect and mechanism of gastrin and its antagonists on colorectal carcinoma

World Journal of Gastroenterology 1999年第5期5卷 408-416页

作者： He SW shen kq He YJ Xie B Zhao YM Third Mil Med Univ Daping Hosp Dept Gen Surg Chongqing 400042 Peoples R China Third Mil Med Univ Inst Surg Res Chongqing 400042 Peoples R China

AIM:To explore the effect and mechanism of gastrin and its antagonists prog lumide and somatostatin on colorectal carcinoma and their clinical ***:A model of transplanted human colonic carcinoma was established from SW480 cell line in gymnomouse *** volume and weight of transplanted carcinoma was observed under the effect of pentagatrin (PG), proglumide (PGL) and octapeptide somotostatin (SMS201-995, SMS). The cAMP content of carcinoma cell was determined by radioimmunoassay and the DNA, protein content and cell cycle were determined by flow-cytometry. The amount of viable cells was determined by MTT colorimetric analysis,IP(3) content was determined by radioimmunoassay, Ca(2+) concentration in cell by fluorometry and PKC activity by isotopic enzymolysis. The expression of gastrin, c-myc, c-fos and rasP21 in 48 cases of colorectal carcinoma tissue was detected by the immuno-cytochemistry SP method. Argyrophilia nucleolar organizer regions was determined with argyrophilia ***:The volume,weight, cAMP, DNA and protein content in carcinoma cell, cell amount and proliferation index of S and G(2)M phase in PG group were all significantly higher than those of control group. When PG was at the concentration of 25mg/L, the amount of viable cells, IP(3) content and Ca(2+) concentration in cell and membrane PKC activity in PG group were significantly higher than those in control group; when PGL was at a concentration of 32mg/L, they dropped to the lowest level in PG (25mg/L)+PGL group, but without significant difference from the control group. The positive expression rate of gastrin, c-myc, c-fos and rasP21 in carcinoma tissue was 39.6%, 54.2%, 47.9% and 54.2% respectively and significantly higher than that in mucosa 3cm and 6cm adjacent to carcinoma tissue and normal colorectal mucosa. The positive expression rate of gastrin of highly differentiated adenocarcinoma group was significantly higher than that of poorly differentiated and mucinous ad

关键词： colorectal neoplasms gastrin proglumide somatostatin IP_3, Ca^(2+) protein kinase C oncogene AgNORs

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Suppressive effect of Armoracia rusticana and three main glucosinolates on heterocyclic amine generation in roasted fish patties and their potential mechanism analyzed by density functional theory

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Food Science and Human Wellness 2024年

作者： Zhuoming Huang Tiantian Huang Hang Su Zihao Yang Jie Luo Qingwu shen Chaoyi Xue Wei Quan College of Food Science and Technology， Hunan Agricultural University State Key Laboratory of Food Science and Resources， Jiangnan University

This study investigated the suppressive effects of Armoracia rusticana (AR) and its three main glucosinolates on both free and bound heterocyclic amines (HAs)， along with their mechanisms of free radical quenching using density functional theory. Fish patties were supplemented with varying concentrations of AR (0.5%～1.5%) and glucosinolates (0.005%～0.015%)， showing a dose-dependent inhibition of HAs and concurrent elimination of free radicals and HAs intermediates. Glucobrassicin demonstrated the highest reactivity， which was verified by frontier orbit analysis and conceptual density functional parameters， consistent with experimental findings. Furthermore， the O-H bond connected to the sulfur atom of glucobrassicin possessed the smallest bond dissociation enthalpy (BDE) value， which indicated that this particular hydrogen atom is most susceptible to react with free radicals. Overall， AR and its glucosinolates， especially glucobrassicin， show promise as natural additives for improving food safety and quality.

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Association of TRMT2B gene variants with juvenile amyotrophic lateral sclerosis

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Frontiers of Medicine 2024年第1期18卷 68-80页

作者： Yanling Liu Xi He Yanchun Yuan Bin Li Zhen Liu Wanzhen Li Kaixuan Li Shuo Tan Quan Zhu Zhengyan Tang Feng Han Ziqiang Wu Lu shen Hong Jiang Beisha Tang Jian Qiu Zhengmao Hu Junling Wang Department of Neurology Xiangya HospitalCentral South UniversityJiangxiNational Regional Center for Neurological DiseasesNanchang330038China Provincial Laboratory for Diagnosis and Treatment of Genitourinary System Disease Department of UrologyXiangya HospitalCentral South UniversityChangsha410078China Department of Orthopedics The First Affiliated HospitalZhejiang University School of MedicineHangzhou310002China Department of Neurology Xiangya HospitalCentral South UniversityChangsha410078China National Clinical Research Center for Geriatric Diseases Xiangya HospitalCentral South UniversityChangsha410008China Key Laboratory of Hunan Province in Neurodegenerative Disorders Central South UniversityChangsha410008China Center for Medical Genetics School of Life SciencesCentral South UniversityChangsha410008China Engineering Research Center of Hunan Province in Cognitive Impairment Disorders Central South UniversityChangsha410078China Hunan International Scientific and Technological Cooperation Base of Neurodegenerative and Neurogenetic Diseases Changsha410078China Hunan Key Laboratory of Molecular Precision Medicine Xiangya HospitalCentral South UniversityChangsha410078China

Amyotrophic lateral sclerosis(ALS)is a fatal neurodegenerative disease characterized by progressive degeneration of motor neurons,and it demonstrates high clinical heterogeneity and complex genetic architecture.A variation within TRMT2B(c.1356G>T;p.K452N)was identified to be associated with ALS in a family comprising two patients with juvenile ALS(JALS).Two missense variations and one splicing variation were identified in 10 patients with ALS in a cohort with 910 patients with ALS,and three more variants were identified in a public ALS database including 3317 patients with ALS.A decreased number of mitochondria,swollen mitochondria,lower expression of ND1,decreased mitochondrial complex I activities,lower mitochondrial aerobic respiration,and a high level of ROS were observed functionally in patient-originated lymphoblastoid cell lines and TRMT2B interfering HEK293 ***,TRMT2B variations overexpression cells also displayed decreased *** conclusion,a novel JALS-associated gene called TRMT2B was identified,thus broadening the clinical and genetic spectrum of ALS.

关键词： TRMT2B amyotrophic lateral sclerosis mitochondrial complex I tRNA methylation reactive oxygen species

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Effect of exogenous free N^(ε)-(carboxymethyl)lysine on diabetes-associated cognitive dysfunction:neuroinflammation,and metabolic disorders

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Food Science and Human Wellness 2024年第5期13卷 2970-2981页

作者： Huang Zhang Lan Mo Xi Chen Mei Li Maiquan Li Yang Xu Maomao Zeng Zhiyong He Qingwu shen Jie Chen Wei Quan College of Food Science and Technology Hunan Agricultural UniversityChangsha 410128China Henan University of Animal Husbandry and Economy Zhengzhou 450046China College of Food Science and Engineering Northwest A&F UniversityYangling 712100China State Key Laboratory of Food Science and Technology Jiangnan UniversityWuxi 214122China

Diabetes-associated cognitive dysfunction has already been attracted considerable *** glycation end products(AGEs)from daily diets are thought to be a vital contributor to the development of this ***,the effect of one of the best-characterized exogenous AGEs N^(ε)-(carboxymethyl)lysine(CML)on cognitive function is not fully *** the present study,diabetical Goto-Kakizaki(GK)rats were treated with free CML for *** was found that oral consumption of exogenous CML significantly aggravated diabetes-associated cognitive dysfunction in behavioral *** details,exogenous CML increased levels of oxidative stress,promoted the activation of glial cells in the brain,up-regulated the release of inflammatory cytokines interleukin-6,inhibited the protein expression of the brain-derived neurotrophic factor and thus led to ***,exogenous CML promoted the amyloidogenesis in the brain of GK rats,and inhibited the expression of ***,several tricarboxylic acid cycle and glutamate-glutamine/γ-aminobutyric acid cycle intermediates including pyruvate,succinic acid,glutamine,glutamate were significantly changed in brain of GK rats treated with exogenous free *** conclusion,exogenous free CML is a potentially noxious compounds led to aggravated diabetes-associated cognitive dysfunction which could be possibly explained by its effects on neuroinflammation,energy and neurotransmitter amino acid homeostasis.

关键词： Diabetes Cognitive dysfunction N^(ε)-(carboxymethyl)lysine Neuroinflammation Metabolomic

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冬小麦保险理赔的高精度气象数据查询研究——以河南省一次大风气象灾害为例

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保险职业学院学报 2024年第1期38卷 65-70页

作者：沈文颖秦乐河南省气象服务中心河南郑州450003 中国气象局河南省农业气象保障与应用技术重点实验室河南郑州450003

为解决河南省冬小麦保险理赔中气象凭证获取渠道不统一、查询效率低、理赔控制难、理赔不规范等问题,本文首先对2018—2021年河南冬小麦保险案例进行分析,发现暴雨、风灾、冻灾和旱灾是河南省主要的农业气象灾害保险理赔类型。基于风灾... 详细信息

为解决河南省冬小麦保险理赔中气象凭证获取渠道不统一、查询效率低、理赔控制难、理赔不规范等问题,本文首先对2018—2021年河南冬小麦保险案例进行分析,发现暴雨、风灾、冻灾和旱灾是河南省主要的农业气象灾害保险理赔类型。基于风灾持续时间短、成灾范围小、保险理赔获取气象凭证查询难度大,本研究以河南省冬小麦风灾为例,对气象站点数据、智能网格实况数据和基于站点数据克里金插值法三种方法进行对比,研究河南冬小麦风灾保险理赔优化查询方法。结果表明:基于站点数据,克里金插值法优于站点数据和智能网格实况数据;当极大风速大于10 m/s时,克里金插值法可以作为未覆盖站点数据的有效补充,在保险理赔查询气象凭证中具有较好的适用性,可提高保险理赔中获取气象凭证的有效性和准确性,为冬小麦农业保险风灾理赔的气象查询提供参考依据。

关键词：保险理赔气象灾害智能网格克里金插值法

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显热回收通风系统在华北肉种鸡舍应用效果研究

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家畜生态学报 2024年第9期45卷 63-68页

作者：申李琰牛晋国张希瑶李惠龙张洁山西农业大学动物科学学院山西太原030032

本试验旨在研究显热回收通风系统(sensible heat recovery ventilation,SHRV)在寒冷地区肉种鸡舍的运行效果,选择两栋同类型的鸡舍,对照舍采用锅炉供热,试验舍关闭7/10的供热管并加装1套SHRV,进行SHRV应用效果和热回收性能评价。结果表... 详细信息

本试验旨在研究显热回收通风系统(sensible heat recovery ventilation,SHRV)在寒冷地区肉种鸡舍的运行效果,选择两栋同类型的鸡舍,对照舍采用锅炉供热,试验舍关闭7/10的供热管并加装1套SHRV,进行SHRV应用效果和热回收性能评价。结果表明,与对照舍相比,试验舍内平均温度降低约0.5℃,差异不显著(P>0.05),相对湿度、CO_(2)浓度、NH_(3)浓度略有升高,差异不显著(P>0.05);两栋鸡舍内不同位置温度和NH 3浓度存在极显著差异(P<0.01);SHRV的显热回收效率为64.96%~78.95%,能效比平均为4.1,运行SHRV每月降低成本1.05万元。研究表明,在该地区运行SHRV能够回收鸡舍排出废热的64%以上的热量,降低养殖成本。

关键词：热回收通风肉种鸡舍环境参数

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外立面形式对高层建筑表面风压影响研究

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应用力学学报 2024年第4期41卷 928-937页

作者：王培杰沈炼韩艳杨瑛王胜德汪阔长沙理工大学土木工程学院长沙410076 长沙学院土木工程学院长沙410022 中国建筑第五工程局有限公司长沙410083

针对当前高层建筑典型外立面形式,以长沙市同有大厦为研究对象,基于SST k-ω湍流模型对增设阳台、不同凹凸外形及不同格栅类型等9种外立面高层建筑表面风压进行了详细分析,明确了不同外立面形式下建筑表面风压分布特性,并结合高层建筑... 详细信息

针对当前高层建筑典型外立面形式,以长沙市同有大厦为研究对象,基于SST k-ω湍流模型对增设阳台、不同凹凸外形及不同格栅类型等9种外立面高层建筑表面风压进行了详细分析,明确了不同外立面形式下建筑表面风压分布特性,并结合高层建筑周围流场信息,揭示了外立面形式对其表面风压的影响机理。结果表明:外立面增设阳台会导致迎风面风压系数最大值增加,背风面风压系数负值最大值减小;同时,阳台会使来流在建筑顶层阳台顶端出现流动分离,在高层建筑迎风面顶部形成局部负压;相比光滑立面,凹、凸立面会导致高层建筑迎风面和背风面表面风压差增加,建筑物靠近顶部区域会出现压力突变现象,且在墙角处会出现较大负值;增设竖向格栅同样会增大高层建筑迎风面和背风面压差,而横向和矩形格栅对结构风荷载影响相对较小。

关键词：高层建筑数值模拟 SST k-ω湍流模型外立面风压系数

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MMPs/pH协同响应的血筒素-普鲁士蓝纳米递药系统联合光热抑制RAFLS细胞增殖和迁移的研究

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中国中药杂志 2024年第11期49卷 2906-2919页

作者：邓雅思谌鑫阳龙梦婷郑豪李斌王炜余黄合湖南中医药大学药学院创新药物研究所中药与民族药创新与发展国际实验室湖南长沙410208

类风湿关节炎(rheumatoid arthritis,RA)关节处于弱酸性环境,关节中RA成纤维样滑膜细胞(RA fibroblast-like synoviocyte,RAFLS)被异常激活,分泌大量基质金属蛋白酶(matrix metalloproteinases,MMPs),细胞膜上CD44受体蛋白被特异性上调。血筒素(xuetongsu,XTS)是土家族药物血筒中抑制RAFLS增殖的主要活性成分,因靶向性差限制了其开发利用。该研究构建了一种可靶向CD44的仿生XTS-普鲁士蓝纳米(Prussian blue nanoparticles,PB NPs)载药递送系统THMPX NPs。THMPX NPs表面有透明质酸(hyaluronic acid,HA)和三聚甘油单硬脂酸酯(triglycerol monostearate,TGMS)-3-氨基苯硼酸(3-aminobenzeneboronic acid,PBA)长链(PBA-TGMS)修饰。炎性RAFLS中过表达的MMPs和H+可协同切割纳米粒表面的PBATGMS,从而暴露HA与CD44相互作用,使THMPX NPs在RAFLS中高度富集,近红外光照射后产热并释放XTS,抑制RAFLS细胞增殖和迁移。表征发现THMPX NPs为形态均一的立方形,粒径为(190.3±4.7)nm,平均电位在(-15.3±2.3)mV,近红外光照射5 min温度可达41.5℃,释药呈现MMPs和H+敏感性;生物安全性考察发现THMPX NPs溶血率小于4%,对正常的RAW264.7和HFLS无细胞毒性;体外摄取实验表明THMPX NPs有明显的RAFLS靶向性;自由基清除实验发现THMPX NPs有优异的自由基清除能力,可清除RAFLS中的活性氧;cell counting kit-8和划痕实验表明THMPX NPs显著抑制RAFLS活力和迁移能力。该研究为开发抗RA中药民族药创新纳米靶向药物提供了思路。

关键词：类风湿关节炎类风湿关节炎成纤维样滑膜细胞血筒素普鲁士蓝透明质酸 MMPs/pH协同响应

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基于HPLC指纹图谱结合化学模式识别以及多指标成分定量评价前胡药材质量

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天然产物研究与开发 2024年第3期36卷 433-443页

作者：谢景秦优唐雪阳沈冰冰王勇庆陈林张水寒湖南省中医药研究院中药资源研究所长沙410013 湖南中医药大学药学院长沙410208

基于HPLC指纹图谱和多指标成分含量测定,并结合化学模式识别,评价不同产地前胡药材质量。采用Agilent SB-C_(18)(4.6 mm×250 mm,5μm)色谱柱,以甲醇-0.5%甲酸水为流动相进行梯度洗脱,流速为0.5 mL/min,检测波长321 nm,建立指纹图谱并对... 详细信息

基于HPLC指纹图谱和多指标成分含量测定,并结合化学模式识别,评价不同产地前胡药材质量。采用Agilent SB-C_(18)(4.6 mm×250 mm,5μm)色谱柱,以甲醇-0.5%甲酸水为流动相进行梯度洗脱,流速为0.5 mL/min,检测波长321 nm,建立指纹图谱并对4个香豆素类成分进行定量,采用聚类分析(hierarchical clustering analysis,HCA)、主成分分析(principal component analysis,PCA)、偏最小二乘法-判别分析(partial least squares discriminant analysis,PLS-DA)对不同产地前胡药材进行质量评价。结果显示16批前胡药材HPLC指纹图谱相似度为0.966~0.999,确定了21个共有峰,共指认了9个成分。通过化学模式识别分析,将16批样品聚类为三类:以安徽宁国、亳州等为主的道地产区前胡,以湖南、贵州、重庆等为主的前胡,以及高海拔云南产区的前胡,并确立8个差异性质量标志物。多指标含量测定不同产地前胡的佛手柑内酯、白花前胡甲素、白花前胡乙素、白花前胡素E含量分别为0.0167~0.1319 mg/g、6.0696~16.2255 mg/g、0.4265~2.0100 mg/g、1.3407~3.1155 mg/g。本研究所建立的指纹图谱和含量测定方法专属性强,稳定性高,分离度良好,结合化学模式可用于前胡药材的质量评价。

关键词：前胡香豆素指纹图谱含量测定化学模式识别

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An explanation to the high efficiency of m-THPC (tempor-fin) used in photodynamic therapy

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Chinese Science Bulletin 2001年第10期46卷 823-827页

作者： Yanwen Chen Lei Li Manhua Zhang Tao shen Chinese Acad Sci Inst Chem Ctr Mol Sci Beijing 100080 Peoples R China

Meso-tetrahydroxylphenyl chlorin (m-THPC) is one of the most efficient prospective sensitizers used in photodynamic therapy (PDT). ESR spectroscopy, fluorescence quenching experiments and cyclic voltammogram measurement were used to study its redox properties. The results showed that the ability of m-THPC generating su-peroxide radical anions was very strong, and the rate constant of m-THPC fluorescence quenching by oxygen kq (O2)=1.46×l010 mol-1 . s-1. The values of fluorescence quenching rate constant of m-THPC by some other electron acceptors, such as methyl viologen (MV2+) and anthraquinone (An), were also measured. And they were kq, (MV2+)=5.51×l09 mol-1 . s-1, kq (An)=7.81×l09 mol-1 .s-1. The oxidation potential of m-THPC was examined to be +0.62 V (vs. NHE) in acetonitrile. All these suggested that m-THPC should be a much stronger electron donor than photofrin, the currently used in clinical photodrug, and may react easily through electron transfer with biological matter to yield various

关键词： meso-tetrahydroxylphenyl chlorin photodynamic therapy redox properties superoxide anion radical fluorescence quenching.

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