检索结果-南通市图书馆

男性不育患者精子DNA中8-羟基脱氧鸟苷水平及与精液参数的相关性

在线全文

学校读者我要写书评

暂无评论

中国男科学杂志 2001年第3期15卷 157-159页

作者：逄兵金泰廙蒋学之 Vivian NG shen hm Ong CN 上海医科大学劳动卫生学教研室上海200032 National University of Singapore shigapore119074

本文应用高压液相电化学 (HPLC EC)技术对 5 2名男性不育患者精子DNA中 8 羟基脱氧鸟苷 (8 OHdG)进行测定 ,同时检测精液量、精子密度、精子活率、正常形态精子率 ,及头部精子畸形等精液参数 ,并进行相关性分析。结果显示 ,当精子DNA中... 详细信息

本文应用高压液相电化学 (HPLC EC)技术对 5 2名男性不育患者精子DNA中 8 羟基脱氧鸟苷 (8 OHdG)进行测定 ,同时检测精液量、精子密度、精子活率、正常形态精子率 ,及头部精子畸形等精液参数 ,并进行相关性分析。结果显示 ,当精子DNA中 8 OHdG超过 10 .5 0时 ,其头部畸形率明显增加。精子DNA中 8 OHdG与精液量 (r =- 0 .4 7,P <0 .0 0 1)、正常形态精子率 (r=- 0 .36 ,P <0 .0 1)成一定的负相关 ,且与头部畸形精子率成正相关 (r =0 .6 9,P <0 .0 0 1)。结果提示 ,精子DNA中 8 OHdG与精液质量存在一定的相关性 ,表明精液质量的下降与精子DNA的损伤存在一定联系 ,而内源及外源性活性氧是可能的原因之一 ,其可能的机制尚需进一步研究。

关键词： 8-羟基脱氧鸟苷精子DNA 精液参数男性不育症

氯化镉影响肾上腺皮质细胞分泌功能的实验研究

在线全文

学校读者我要写书评

暂无评论

中国职业医学 1999年第6期26卷 1-4页

作者：杨杏芬庄志雄魏青杜柳涛赵敏陈铁江 hm shen CN Ong 中山医科大学公共卫生学院广州市510080 深圳市卫生防疫站毒理科新加坡国立大学社区职业与家庭医学系

目的　探讨ＣｄＣｌ２对肾上腺皮质细胞分泌功能、细胞［Ｃａ２＋］ｉ调控和能量代谢的毒作用，为深入了解金属内分泌毒性机制提供依据。方法　体外分离培养原代豚鼠肾上腺皮质细胞，采用Ｆｌｕｏ－３／ＡＭ和Ｆｕｒａ－ｒｅｄ... 详细信息

目的　探讨ＣｄＣｌ２对肾上腺皮质细胞分泌功能、细胞［Ｃａ２＋］ｉ调控和能量代谢的毒作用，为深入了解金属内分泌毒性机制提供依据。方法　体外分离培养原代豚鼠肾上腺皮质细胞，采用Ｆｌｕｏ－３／ＡＭ和Ｆｕｒａ－ｒｅｄ／ＡＭ联合标记、流式细胞术测定细胞［Ｃａ２＋］ｉ变化，化学发光法测定ＡＴＰ水平，ＲＩＡ法测定培养细胞皮质醇分泌水平。结果　①ＣｄＣｌ２可浓度依赖性地抑制肾上腺皮质细胞分泌皮质醇的功能，且随时间延长抑制效应愈趋明显，呈现明显的剂量效应和时间效应关系，剂量和时间对抑制效应存在交互作用；②细胞［Ｃａ２＋］ｉ随ＣｄＣｌ２浓度增加而持续上升，表现出剂量效应关系。③细胞ＡＴＰ水平随ＣｄＣｌ２浓度增加而降低，有明显的剂量效应和时间效应关系，而且存在交互作用。结论　一定浓度ＣｄＣｌ２对肾上腺皮质细胞［Ｃａ２＋］ｉ调控、能量代谢和肾上腺皮质激素合成分泌具有直接毒作用。

关键词：氯化镉肾上腺皮质激素细胞游离钙水平

Natural killer cell membrane doped supramolecular nanoplatform with immuno-modulatory functions for immuno-enhanced tumor phototherapy

在线全文

学校读者我要写书评

暂无评论

Chinese Chemical Letters 2024年第10期35卷 205-212页

作者： Ying Gao Rong Zhou Qiwen Wang Shaolong Qi Yuanyuan Lv Shuang Liu Jie shen Guocan Yu Institute of Pharmaceutics College of Pharmaceutical SciencesZhejiang UniversityHangzhou 310058China Key Laboratory of Novel Targets and Drug Study for Neural Repair of Zhejiang Province School of MedicineHangzhou City UniversityHangzhou 310015 China Department of Cardiology The First Afiliated HospitalZhejiang University School of MedicineHangzhou 310003China MEKey Laboratoryof Bioorganic Phosphorus Chemistry Chemical Bology epartmentof ChemtryTsinghua UniversityBeijing04Chin Emergency Department State Key Laboratory of Complex Severe and Rare DiseasesPeking Union Medical College HospitalChinese Academy of Medical Science and Peking Union Medical CollegeBeijing 100730China

Photodynamic therapy(PDT)has garnered significant attention as a promising approach to cancer therapy,harnessing the combined benefits of localized light treatment and the accompanying host immune *** this study,we engineered an immuno-enhanced PDT nanoplatform,denoted as hm@p-MOF(hybrid membrane@porphyrin-metal organic framework).The core porphyrin-MOF was cloaked with a hybrid membrane derived from B16F10 cancer cells and NK cells,resulting in enhanced *** both in vitro and in vivo experiments,our finding demonstrated that the hybrid membrane conferred dual targeting capabilities to the nanoplatform,leveraging the unique properties of the B16F10 membrane and NK membrane to augment immunogenic cell death(ICD)induced by photodynamic ***,in conjunction with the immunomodulatory functions of the NK cell membrane,we observed an expansion of in situ immune infiltration leading to a systemic immune *** hm@p-MOF nanoplatform exhibited the capacity to not only inhibit the growth of mouse melanoma but also suppress *** innovative hm@p-MOF nanoplatform present a viable strategy to enhance phototherapeutic efficacy for both localized and metastatic *** provides a direction for the fabrication of biomimetic nanomedicines possessing immuno-modulatory function.

关键词： Biomimetic Cell membrane Metal-organic framework Phototherapy Tumor targeting

Intravitreal injection practice patterns among Chinese ophthalmologists

在线全文

学校读者我要写书评

暂无评论

International Journal of Ophthalmology(English edition) 2024年第9期17卷 1717-1722页

作者： Li Su Yin-Chen shen Hong Li Bo Mou Kun Liu Xun Xu Department of Ophthalmology Shanghai General HospitalSchool of MedicineShanghai Jiao Tong UniversityShanghai 200080China National Clinical Research Center for Eye Diseases Shanghai 200080China Shanghai Key Laboratory of Ocular Fundus Diseases Shanghai 200080China Shanghai MicroCall Information Technology Co. Ltd.Shanghai 200436China

AIM:To describe the practice patterns of intravitreal injections(IVIs)among ophthalmologists in ***:This was a cross-sectional online *** who had performed accumulated more than 100 injections were contacted by the Brightness Center,a hospital-based national network,to complete an anonymous,24-question,internet-based *** were surveyed on practices in injection techniques,pre-,and post-injections ***:A total of 333 ophthalmologists from 28 provinces/municipalities/autonomous regions responded to the survey(50.68%response rate).The 91.29%of the respondents evaluated systemic risk factors by medical history,electrocardiogram(ECG)and blood *** the respondents used pre-injection prophylactic *** checked intraocular pressure(IOP,99.1%)and blood pressure(96.1%)before injections.A majority of the respondents performed injections in the operating room(98.8%),wore masks(99.7%),gloves(99.4%)and sterile surgical clothing(96.1%),performed topical anesthetics(97.9%),and applied povidone-iodine(95.8%)*** 61.26%of the respondents dilated *** half of the respondents(51.05%)performed bilateral injections in the same *** temporal quadrant(40.54%)was the most frequent site of *** three quarters used 30-gauge *** respondents(97.9%)measured the site of injection from *** than half(53.45%)performed conjunctiva displacement prior to *** 32.43%of the respondents checked IOP post-injection and 87.99%physicians checked hand motion(hm)or counting fingers(CF)after injection,while 36.94%observed optic nerve *** participants used topical antibiotics *** physicians(91.89%)reviewed patients on the following ***:This study provides a description of the real-world practice patterns in IVIs in China and offers critical information regarding education and training of ophthalmologists and amendment of local society guideli

关键词： intravitreal injections practice pattern topical antibiotics Chinese ophthalmologists

靶器官毒理学和生物学标志——铅对肾上腺皮质细胞线粒体的氧化损伤

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

毒理学杂志 2001年第2期 111-111页

作者：杨杏芬庄志雄魏青凌莉范瑞泉 shen hm Ong CN 中山医科大学公共卫生学院卫生毒理学教研室!广州510089

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

铅对肾上腺皮质细胞线粒体的氧化损伤

中山医科大学学报 2001年第1期22卷 14-18页

作者：杨杏芬庄志雄魏青凌莉范瑞泉 shen H M Ong C N 中山医科大学公共卫生学院卫生毒理学教研室广东广州510089

【目的】研究铅对肾上腺皮质细胞氧化应激和线粒体功能的影响 ,为了解其肾上腺皮质毒作用机制提供依据。【方法】原代分离培养豚鼠肾上腺皮质细胞 ,以 0、6 2 5、12 5、2 5、5 0、10 0 μmol/L醋酸铅 (PbAc)处理细胞 ,观察PbAc诱导肾... 详细信息

【目的】研究铅对肾上腺皮质细胞氧化应激和线粒体功能的影响 ,为了解其肾上腺皮质毒作用机制提供依据。【方法】原代分离培养豚鼠肾上腺皮质细胞 ,以 0、6 2 5、12 5、2 5、5 0、10 0 μmol/L醋酸铅 (PbAc)处理细胞 ,观察PbAc诱导肾上腺皮质细胞活性氧 (ROS)产生和线粒体损伤作用。ROS检测采用荧光分光光度法 ,线粒体膜电位 (MMP)和细胞存活状态采用Rh12 3和PI双标记、流式细胞术检测 ,细胞ATP水平采用化学发光法测定。【结果】PbAc染毒后肾上腺皮质细胞ROS形成水平随剂量增加而增加 ,具有剂量效应关系 [^y=4 16 +10 2 1× 1g(x+1) ,P <0 0 1,R2 =0 6 41];线粒体膜电位呈剂量依赖性降低 ,Rh12 3的平均荧光强度 (MFI)在 6 2 5～ 10 0 μmol/L各剂量组依次为 1 0 1,0 94,0 96 ,0 95和 0 91,与对照组(1 35 )比较具有显著性差异 (P <0 0 1) ;染毒后细胞死亡率轻度增加 ,与对照组 (1 0 2 % )比较 ,5 0 μmol/L和 10 0 μmol/L的PbAc组分别为 3 16 %和 3 40 % ,差异具有显著性 (P <0 0 5 ) ;ATP水平降低与PbAc剂量之间存在剂量效应关系 [^y =2 12 96 5 7- 5 15 92 5× 1g(x +1) ,P <0 0 1,R2 =0 5 6 8],剂量和时间对ATP水平的影响呈协同抑制作用 (P <0 0 1)。【结论】线粒体氧化应激介导肾?

关键词：铅肾上腺皮质细胞氧化应激线粒体 ATP 细胞毒性氧化损伤

A Model for Liquid-Diffusion Catalytically Grown Fibrous Carbon Material

在线全文

学校读者我要写书评

暂无评论

Journal of Materials Science & Technology 1999年第1期15卷 1-4页

作者： Zhang, WG Cheng, hm Su, G Fan, YY Li, F shen, ZH Zhou, BL Chinese Acad Sci Met Res Inst Shenyang 110015 Peoples R China

A mechanism for the catalytically growth of vapor grown fibrous carbon (VGFC) preparing from the floating catalyst method has been proposed, in which liquid diffusion-determined diffusion of carbon atoms through catalyst particles is described mathematically, and the physical and chemical properties of the catalyst particles during the growth process are taken into account. From the model the growth rate and grown length of VGFC are calculated theoretically which coincides with the experimental data; and the necessity of reducing gas such as hydrogen in preparing VGFC is explained as to reduce Fe3C, which acts as intermediate catalyst shown by the model. Fe3C formed on the surface of gamma-Fe will stop the growth of carbon fiber if they can not be reduced at once. Additionally, the growth temperature and diameter of VGFC are also discussed in the paper.

关键词： FILAMENTOUS CARBON NICKEL FIBERS IRON DECOMPOSITION CATALYSTS

在线全文

学校读者我要写书评

暂无评论

红色无角菱新品种‘南湖红菱’

园艺学报 2024年第2期51卷 455-456页

作者：姚祥坦沈亚强袁晔王瑞森沈盟权新华嘉兴市农业科学研究院浙江嘉兴314016

‘南湖红菱’是以地方品种‘水红菱’为母本,‘南湖菱’为父本,通过有性杂交经系谱法选育获得的红色无角菱新品种。果实元宝形,果皮浅紫红色,光泽度好,四角退化,果肉乳白色,生食爽脆,熟食粉糯,果形、口感与‘南湖菱’类似。其产量与‘... 详细信息

‘南湖红菱’是以地方品种‘水红菱’为母本,‘南湖菱’为父本,通过有性杂交经系谱法选育获得的红色无角菱新品种。果实元宝形,果皮浅紫红色,光泽度好,四角退化,果肉乳白色,生食爽脆,熟食粉糯,果形、口感与‘南湖菱’类似。其产量与‘南湖菱’相当,平均12 t·hm^(-2)左右。

关键词：菱角无角菱选育红皮品种

浙江省新垦耕地土壤培肥和覆膜节水栽培对土壤肥力和水稻产量的影响

在线全文

学校读者我要写书评

暂无评论

中国稻米 2024年第4期30卷 81-85页

作者：沈菁张莉赵洪阳沈量周政法王未英绍兴市粮油作物技术推广中心浙江绍兴312000 上海市农业生物基因中心上海201203 嵊州市农业技术推广中心浙江嵊州312400 嵊州市三界镇事业综合服务中心浙江嵊州312400

新垦耕地存在土壤肥力差、生产力低等突出问题。本研究通过多年多点田间试验,探究了适宜浙江省新垦耕地改造的水稻高产栽培技术。结果表明,受新垦农田肥力影响,参加水稻品种筛选试验的8个品种中,旱优73较适合在新垦耕地种植,产量较高;... 详细信息

新垦耕地存在土壤肥力差、生产力低等突出问题。本研究通过多年多点田间试验,探究了适宜浙江省新垦耕地改造的水稻高产栽培技术。结果表明,受新垦农田肥力影响,参加水稻品种筛选试验的8个品种中,旱优73较适合在新垦耕地种植,产量较高;在新垦耕地施用有机物料可以有效提升土壤地力水平,增加水稻产量;在新垦农田采用覆膜节水栽培并施用有机物料明显提升水稻产量,其中,覆膜+施用巴贝蚕沙(11.25 t/hm2)处理下,旱优73的产量比不施肥+不覆膜处理显著增加36.76%。可见,在新垦耕地上种植水稻,通过施用有机物料培肥和覆膜节水栽培,能达到提高土壤肥力和水稻产量的目的。

关键词：水稻新垦耕地有机肥覆膜节水栽培技术产量