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A practical guide for fast implementation of SNARE-mediated liposome fusion

Biophysics Reports 2024年第1期10卷 31-40页

作者： shen wang Cong Ma Key Laboratory of Molecular Biophysics of the Ministry of Education College of Life Science and TechnologyHuazhong University of Science and Technology

Soluble N-ethylmaleimide-sensitive factor attachment protein receptor(SNAER) family proteins are the engines of most intra-cellular and exocytotic membrane fusion pathways(Jahn and Scheller 2006).Over the past two decades, in-vitro liposome fusion has been proven to be a powerful tool to reconstruct physiological SNARE-mediated membrane fusion processes(Liu et al. 2017). The reconstitution of the membrane fusion process not only provides direct evidence of the capability of the cognate SNARE complex in driving membrane fusion but also allows researchers to study the functional mechanisms of regulatory proteins in related pathways(Wickner and Rizo 2017). Heretofore, a variety of delicate methods for in-vitro SNARE-mediated liposome fusion have been established(Bao et al. 2018;Diao et al. 2012; Duzgunes 2003; Gong et al. 2015; Heo et al. 2021; Kiessling et al. 2015; Kreye et al.2008; Kyoung et al. 2013; Liu et al. 2017; Scott et al. 2003). Although technological advances have made reconstitution more physiologically relevant, increasingly elaborate experimental procedures, instruments, and data processing algorithms nevertheless hinder the non-experts from setting up basic SNARE-mediated liposome fusion assays. Here, we describe a low-cost, timesaving, and easy-to-handle protocol to set up a foundational in-vitro SNARE-mediated liposome fusion assay based on our previous publications(Liu et al. 2023; wang and Ma 2022). The protocol can be readily adapted to assess various types of SNARE-mediated membrane fusion and the actions of fusion regulators by using appropriate alternative additives(e.g., proteins, macromolecules, chemicals, etc.). The total time required for one round of the assay is typically two days and could be extremely compressed into one day.

关键词： Liposome fusion SNARE In-vitro reconstitution Protocol

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A"messenger zone hypothesis"based on the visual three-dimensional spatial distribution of motoneurons innervating deep limb muscles

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Neural Regeneration Research 2024年第7期19卷 1559-1567页

作者： Chen Huang shen wang Jin Deng Xinyi Gu Shuhang Guo Xiaofeng Yin MoE Key Laboratory for Trauma Treatment and Nerve Regeneration Peking UniversityBeijingChina Department of Orthopedics and Trauma Peking University People’s HospitalBeijingChina

Coordinated contraction of skeletal muscles relies on selective connections between the muscles and multiple classes of the spinal motoneuro *** r,current research on the spatial location of the spinal motoneurons innervating differe nt muscles is *** this study,we investigated the spatial distribution and relative position of different motoneurons that control the deep muscles of the mouse hindlimbs,which were innervated by the obturator nerve,femoral nerve,inferior gluteal nerve,deep pe roneal nerve,and tibial *** were visualized by combining a multiplex retrograde tracking technique compatible with three-dimensional imaging of solvent-cleared o rgans(3DISCO)and 3-D imaging technology based on lightsheet fluorescence microscopy(LSFM).Additionally,we propose the hypothesis that"messenger zones"exist as interlaced areas between the motoneuron pools that dominate the synergistic or antagonist muscle *** hypothesize that these interlaced neurons may participate in muscle coordination as messenger *** revealed the precise mutual positional relationships among the many motoneurons that innervate different deep muscles of the *** only do these findings update and supplement our knowledge regarding the overall spatial layout of spinal motoneurons that control mouse limb muscles,but they also provide insights into the mechanisms through which muscle activity is coordinated and the architecture of motor circuits.

关键词： 3-D imaging motoneurons multiple retrograde tracing muscle coordination skeletal muscle spatial distribution optical tissue clearing

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Evolution and Application of Sealing Ability of Gypsum Caprocks under Temperature-Pressure Coupling:An Example of the ZS5 Well in the Tazhong Area of the Tarim Basin

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Acta Geologica Sinica(English Edition) 2024年第1期98卷 168-184页

作者： LIU Hua ZHAO Shan YANG Xianzhang ZHU Yongfeng wang shen ZHANG Ke National Key Laboratory of Deep Oil and Gas China University of Petroleum(East China)QingdaoShandong 266580China School of Geosciences China University of Petroleum(East China)QingdaoShandong 266580China Laboratory for Marine Mineral Resources National Laboratory for Marine Science and TechnologyQingdaoShandong 266071China PetroChina Tarim Oil Field Company KorlaXinjiang 841001China

Gypsum caprocks'sealing ability is affected by temperature-pressure *** to the limitations of experimental conditions,there is still a lack of triaxial stress-strain experiments that simultaneously consider changes in temperature and pressure conditions,which limits the accuracy of the comprehensive evaluation of the brittle plastic evolution and sealing ability of gypsum rocks using temperature pressure *** stress-strain tests were utilized to investigate the differences in the evolution of the confinement capacity of gypsum rocks under coupled temperaturepressure action and isothermal-variable pressure action on the basis of sample feasibility *** to research,the gypsum rock's peak and residual strengths decrease under simultaneous increases in temperature and pressure over isothermal pressurization experimental conditions,and it becomes more *** reduces the amount of time it takes for the rock to transition from brittle to *** temperature is taken into account,both the brittle–plastic transformation's depth limit and the lithological transformation of gypsum rocks become shallower,and the evolution of gypsum rocks under variable temperature and pressure conditions is more complicated than that under isothermal *** sealing ability under the temperature-pressure coupling is more in line with the actual geological context when the application results of the Well#ZS5 are *** provides a theoretical basis for precisely determining the process of hydrocarbon accumulation and explains why the early hydrocarbon were not well preserved.

关键词： temperature-pressure coupling gypsum caprocks brittle-plastic evolution sealing capacity Tarim Basin

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Ultrastable graphene isolated Au Ag nanoalloy for SERS biosensing and photothermal therapy of bacterial infection

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Chinese Chemical Letters 2024年第3期35卷 198-203页

作者： shengkai Li Zhiyang Li Qing Hao shen wang Yanxia Yang Jieqiong Xu Zhiwei Yin Liang Zhang Zhuo Chen Molecular Science and Biomedicine Laboratory(MBL) State Key Laboratory of Chemo/Biosensing and ChemometricsCollege of Chemistry and Chemical EngineeringCollege of BiologyAptamer Engineering Center of Hunan ProvinceHunan UniversityChangsha 410082China School of Chemistry and Chemical Engineering/Guangzhou Key Laboratory for Clean Energy and Materials/Key Laboratory for Water Quality and Conservation of the Pearl River Delta Ministry of EducationGuangzhou UniversityGuangzhou 510006China

Plasmonic metal nanomaterials with intrinsic surface–enhanced Raman scattering(SERS)and photothermal properties,especially AuAg nanoalloys with both the outstanding merits of Au and Ag nanocrystals,show huge application prospects in bacterial ***,the direct exposure of AuAg nanoalloys in external conditions probably cause undesirable reactions and poisonous metal ion leakage during SERS detection and photothermal antibacterial therapy process,which severely hinder bacterial theranostics ***,we report an ultrastable graphene–isolated AuAg nanoalloy(GAA)with AuAg core confined in few–layer graphitic shell as a versatile platform for bacterial detection and *** encapsulation of graphene ensures the good stability of AuAg core,that its superior SERS and photothermal properties are therefore further *** is used for SERS detection of two vital bacterial biomarkers(including corrosive cyanide and pyocyanin),exhibiting good SERS quantitative and multiplexing *** is further used for photothermal antibacterial therapy application,and ultrahigh antibacterial efficacies for both Gram–negative Escherichia coli and Gram–positive Staphylococcus aureus are achieved under 808 nm laser *** work proposes a valuable method to develop robust bacterial theranostic platform.

关键词： Localized surface plasmon resonance Graphitic shell isolated AuAg nanoalloy(GAA) Surface–enhanced Raman scattering(SERS) Bacterial biomarker detection Photothermal antibacterial therapy

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Sulfonated poly(aryl ether ketone sulfone)modified by polyoxometalates LaW_(10) clusters for proton exchange membranes with high proton conduction performance

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Tungsten 2024年第2期6卷 454-464页

作者： Meng-Long Liu Xu Han Wen-Wen He Feng-Yu Jiang Fang Ji wang-wang shen Tao Zhou Jing-Mei Xu Ya-Qian Lan School of Chemistry and Life Science Changchun University of TechnologyChangchun 130012China Advanced Institute of Materials Science Changchun University of TechnologyChangchun 130012China School of Chemistry South China Normal UniversityGuangzhou 510006China

Polyoxometalates(POMs)are classified as solid superacids which can exhibit notable proton conductivity,making them a promising functional inorganic filler for enhancing the proton conductivity of proton exchange membranes(PEMs).In this study,a series of hybrid membranes were obtained by molecular-level hybridization of Weakley-type POM Na_(7)H_(2)LaW_(10)O_(36)(LaW_(10))clusters into sulfonated poly(aryl ether ketone sulfone)(SPAEKS).All hybrid membranes exhibited greater proton conductivity than the pristine membrane in the 30–80℃temperature *** the doping amount of LaW 10 reached 7 wt.%,the proton conductivity of M-LaW 10^(-7)achieved 64 mS·cm^(−1)at 80℃.Lanthanide ions'high coordination number property and variable coordination environment can aid to attract more water molecules from the *** 10 and these bound water can construct denser hydrogen bonds with–SO_(3)H of *** intensive hydrogen bonds will facilitate the constitution of more continuous proton transport channels,and improve the proton conductivity of the hybrid *** work off ers a fresh approach to using POMs containing rare-earth components in PEMs.

关键词： Polyoxometalates Rare-earth elements Sulfonated poly(arylene ether ketone sulfone) Proton exchange membranes

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全地下雨水泵站及初雨调蓄池工程设计

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中国给水排水 2024年第2期40卷 82-86页

作者：王珅上海市城市建设设计研究总院<集团>有限公司上海200125

为保障区域防汛安全和内涝防治,上海市桃浦科技智慧城在周边环境复杂的建设条件下设计建设了全地下式合建的泵站及调蓄池,雨水泵站设计流量为19.17 m^(3)/s,调蓄池设计规模为10000 m^(3)。该项目在实现提标和控污功能的前提下,将传统的... 详细信息

为保障区域防汛安全和内涝防治,上海市桃浦科技智慧城在周边环境复杂的建设条件下设计建设了全地下式合建的泵站及调蓄池,雨水泵站设计流量为19.17 m^(3)/s,调蓄池设计规模为10000 m^(3)。该项目在实现提标和控污功能的前提下,将传统的市政设施有机融入景观绿地,采用多系统、多功能的集成布置和创新的进、出水控制方式,并利用模型软件进行辅助设计。雨水泵站建成后将区域排水能力从14.00 m^(3)/s提升至33.17 m^(3)/s,暴雨重现期由1年一遇提升至5年一遇;初雨调蓄池建成后预计年削减COD约100 t、TN约5 t,能有效控制排入河道的污染物总量。

关键词：全地下雨水泵站初雨调蓄池合建

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基于灰色关联和系统聚类的大连市环境质量影响因素分析

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中国环境监测 2024年第2期40卷 152-157页

作者：苏静王甡辽宁省大连生态环境监测中心辽宁大连116023

采用灰色关联度和系统聚类分析方法,定量分析大连市社会、经济、自然、人口、能源资源和污染排放等有代表性的指标与生态环境质量的关联程度。结果表明,大连市社会经济发展与环境质量关联度为中等,经济指标与环境质量指标关联度较强,社... 详细信息

采用灰色关联度和系统聚类分析方法,定量分析大连市社会、经济、自然、人口、能源资源和污染排放等有代表性的指标与生态环境质量的关联程度。结果表明,大连市社会经济发展与环境质量关联度为中等,经济指标与环境质量指标关联度较强,社会、人口、污染排放指标关联度中等,能源和自然指标关联度较弱。金普新区、甘井子区和长兴岛是潜在环境风险区域,需要重点关注环境质量变化趋势。普兰店区、瓦房店市和庄河市虽然属于低排放区域,但环境质量风险相对较大,应进一步加强环境风险管控。

关键词：灰色关联度系统聚类分析环境质量

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数字经济经营者集中审查的挑战及风险应对:一个DSCP范式的分析

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社会科学研究 2024年第2期 75-86页

作者：王申易芳中国政法大学数据法治研究院北京100088 北京工商大学经济学院北京100048

经营者集中审查是重要的事前竞争监管制度,具有预防垄断风险的功能。数字经济的发展为企业赋能的同时,也为经营者集中审查带来巨大挑战。数字经济的经营者集中审查的制度风险是系统性的,需要全面梳理和系统建构,而数据要素作用是系统性... 详细信息

经营者集中审查是重要的事前竞争监管制度,具有预防垄断风险的功能。数字经济的发展为企业赋能的同时,也为经营者集中审查带来巨大挑战。数字经济的经营者集中审查的制度风险是系统性的,需要全面梳理和系统建构,而数据要素作用是系统性制度风险的源头。数字经济经营者集中审查的“数据—结构—行为—政策”(DSCP)分析范式,以数据要素为核心,提炼出数据要素性、结构复杂性和行为不确定性三组风险点。数字经济经营者集中审查制度应当抓住数据这一关键要素,针对数字经济领域的新特征和新问题,围绕审查范围、竞争分析和救济设计方面的制度缺口,进行顶层设计和政策应对。

关键词：经营者集中数据要素审查制度风险应对 DSCP分析范式

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中小学校内部治理的“碎片化”表现及其改进

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教育科学研究 2024年第3期 43-50页

作者：王申张爽首都师范大学教师教育学院 100048 首都师范大学教育学院 100048

中小学校内部治理“碎片化”现象制约学校高质量发展。“碎片化”具体表现为领导决策系统分散、隔离、对立,贯彻执行系统各自为政,民主监督系统缺少权力实现机制,各系统之间沟通交流不顺畅、信息共享不对称。“碎片化”的原因在于个体... 详细信息

中小学校内部治理“碎片化”现象制约学校高质量发展。“碎片化”具体表现为领导决策系统分散、隔离、对立,贯彻执行系统各自为政,民主监督系统缺少权力实现机制,各系统之间沟通交流不顺畅、信息共享不对称。“碎片化”的原因在于个体层面采取缺乏规约的“逐利”行动,组织层面过度集权化和部门间互联互通不够,个体与组织交互层面的制度机制缺乏协调与整合,以及信息资源受到控制和垄断。基于整体性治理理念,应当从三个方面应对“碎片化”现象:一是明确治理目标,树立整体价值观念;二是凝聚多元力量,实现协同共治;三是建构有机融合的正式与非正式制度,促进信息资源高效整合与开放共享。

关键词：中小学校内部治理碎片化整体性治理

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全麦粉加工及其应用研究进展

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粮食与油脂 2024年第7期37卷 1-4,16页

作者：沈汪洋武汉轻工大学食品科学与工程学院湖北武汉430023 大宗粮油精深加工省部共建教育部重点实验室湖北武汉430023

全麦粉和全麦食品的口感、加工品质及储存稳定性等方面存在一些问题,一定程度限制了其应用。基于此,从营养特性、加工和应用研究方面,对目前全麦粉的研究现状进行综述,以期为全麦粉和全麦食品的研究开发提供参考。

关键词：全麦粉营养工艺应用

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